FastQCFastQC Report
Sat 18 Jun 2016
SRR3551536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643429
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70801.1003545068686678No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC12750.1981570616182982No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC10710.1664519317593705No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC8850.13754431335858347No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG7660.11904965427420897No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7550.11734006393867855No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT7410.11516422169345801TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG6610.10273083743505498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG207.030968E-445.0000041
CGGTCTA302.1640062E-645.00000431
GATTCGT207.030968E-445.00000411
CGTTTAT207.030968E-445.00000439
GTTAGCG253.888884E-545.01
GCGTAAG502.1827873E-1145.01
CGTTTTT28000.041.7857131
TCGGCGT1450.040.3448264
GCTAGCG451.925946E-840.01
TAGTGCG403.4550612E-739.3750041
CGTTTGG403.4550612E-739.3750042
AATGGGC1550.039.1935464
CGACGGT356.2440013E-638.5714328
CACGACG356.2440013E-638.5714326
AAATGCG356.2440013E-638.571431
ACGGTCT356.2440013E-638.5714330
CGTAAGG1000.038.252
ATGGGCG1550.037.7419365
CGTTCAT601.546141E-1037.50000417
TACGAAT601.546141E-1037.50000412