##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643429
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.419872278060204	33.0	31.0	34.0	31.0	34.0
2	32.54759888037375	34.0	31.0	34.0	31.0	34.0
3	32.536578239401706	34.0	31.0	34.0	31.0	34.0
4	36.032485324721144	37.0	35.0	37.0	35.0	37.0
5	36.12977655654315	37.0	35.0	37.0	35.0	37.0
6	36.148064510614226	37.0	36.0	37.0	35.0	37.0
7	36.26486216816463	37.0	37.0	37.0	35.0	37.0
8	36.19129694185372	37.0	37.0	37.0	35.0	37.0
9	38.0530625756688	39.0	39.0	39.0	35.0	39.0
10	37.71782434425554	39.0	37.0	39.0	35.0	39.0
11	37.72432078753056	39.0	37.0	39.0	35.0	39.0
12	37.68447956184754	39.0	37.0	39.0	35.0	39.0
13	37.6754125163771	39.0	37.0	39.0	35.0	39.0
14	39.024992656532426	40.0	39.0	41.0	35.0	41.0
15	39.09196974335941	40.0	39.0	41.0	35.0	41.0
16	39.105344956475385	40.0	39.0	41.0	35.0	41.0
17	39.065247292242034	40.0	38.0	41.0	35.0	41.0
18	38.95447516353786	40.0	38.0	41.0	35.0	41.0
19	38.848956139682855	40.0	38.0	41.0	35.0	41.0
20	38.74799084281249	40.0	38.0	41.0	35.0	41.0
21	38.65216830450601	40.0	38.0	41.0	35.0	41.0
22	38.651723811018776	40.0	38.0	41.0	35.0	41.0
23	38.617897234970755	40.0	38.0	41.0	35.0	41.0
24	38.54476562293586	40.0	38.0	41.0	35.0	41.0
25	38.422742835650865	40.0	37.0	41.0	35.0	41.0
26	38.446936647244684	40.0	37.0	41.0	35.0	41.0
27	38.439970843713915	40.0	37.0	41.0	35.0	41.0
28	38.36092715746415	40.0	37.0	41.0	35.0	41.0
29	38.32318406537473	40.0	37.0	41.0	35.0	41.0
30	38.14860380865643	40.0	37.0	41.0	34.0	41.0
31	38.07138627571962	40.0	37.0	41.0	34.0	41.0
32	37.93097140477038	40.0	37.0	41.0	34.0	41.0
33	37.7906606634143	40.0	37.0	41.0	33.0	41.0
34	37.634542428146695	40.0	37.0	41.0	33.0	41.0
35	37.52974298640565	40.0	36.0	41.0	33.0	41.0
36	37.446714400501065	40.0	36.0	41.0	33.0	41.0
37	37.38154170856458	40.0	36.0	41.0	33.0	41.0
38	37.258309774660454	40.0	36.0	41.0	33.0	41.0
39	37.27300292650782	40.0	36.0	41.0	33.0	41.0
40	37.19526629977822	40.0	35.0	41.0	33.0	41.0
41	37.11857252315329	40.0	35.0	41.0	32.0	41.0
42	37.040876304922534	40.0	35.0	41.0	32.0	41.0
43	37.00603951640352	40.0	35.0	41.0	32.0	41.0
44	36.94530554264728	40.0	35.0	41.0	32.0	41.0
45	36.85087865172381	39.0	35.0	41.0	32.0	41.0
46	36.798423757710644	39.0	35.0	41.0	31.0	41.0
47	36.736872288939416	39.0	35.0	41.0	31.0	41.0
48	36.66895648160092	39.0	35.0	41.0	31.0	41.0
49	36.60750447990377	39.0	35.0	41.0	31.0	41.0
50	36.527355776628035	39.0	35.0	41.0	31.0	41.0
51	35.7143196840677	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	9.0
11	23.0
12	10.0
13	15.0
14	20.0
15	28.0
16	45.0
17	58.0
18	128.0
19	211.0
20	290.0
21	543.0
22	873.0
23	1244.0
24	2134.0
25	3448.0
26	5291.0
27	6451.0
28	6585.0
29	6760.0
30	7344.0
31	8455.0
32	10251.0
33	14764.0
34	28161.0
35	41937.0
36	41160.0
37	62088.0
38	121878.0
39	272823.0
40	385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.16507027193366	20.531558260507378	24.116102942204968	14.187268525354002
2	35.034323911418355	24.17251942327747	23.59607664559726	17.197080019706913
3	32.35368626530666	23.05491359575027	28.378888735198444	16.212511403744625
4	28.92284929650358	25.05591137483701	27.48135380904498	18.53988551961444
5	24.776315646326168	30.16976232031817	25.546408383831004	19.507513649524654
6	25.44647505785409	34.674377437137586	25.29618652563064	14.58296097937768
7	76.98129863590233	6.769822311397217	11.395352090129602	4.853526962570851
8	78.06378015289954	6.236740961318187	10.918687221123076	4.780791664659193
9	71.16542773173109	8.410718198899957	13.426345408739737	6.997508660629223
10	36.09722284820858	25.66996513989889	22.428271029126755	15.804540982765774
11	28.791677092577423	24.393989080380273	26.409440668667404	20.404893158374897
12	25.047052588552894	21.964661213591555	32.22500073823219	20.76328545962336
13	24.297474935074423	22.23104647132784	33.50532848224124	19.966150111356498
14	21.760909129056973	25.30815365797936	31.543651280871703	21.387285932091963
15	20.916526920608177	24.827292521785623	33.63867652841262	20.617504029193586
16	22.996787524342235	23.740148485691506	31.605507367557262	21.657556622409
17	23.081023702692917	24.055490193945253	29.812924192102003	23.05056191125983
18	22.608399683570372	24.634575065780375	31.39320733134503	21.36381791930423
19	23.490548296704063	25.271008922507377	29.54871477661094	21.689728004177617
20	23.227582219638844	25.541124195521185	29.976423195100004	21.25487038973997
21	23.75817689286619	25.617589508710363	29.96119229938346	20.66304129903999
22	22.487795856263862	24.502314940731612	29.360193587792903	23.649695615211623
23	22.07469666427842	25.15646637002684	30.446249702764405	22.322587262930334
24	21.922387707112982	25.386639396110528	30.372581901033367	22.31839099574312
25	22.537373975994242	25.92904578438336	29.179132429529908	22.35444781009249
26	21.832867340452484	25.96976511782963	29.058528602223397	23.13883893949449
27	23.156711929365944	25.48750522590682	29.979531541164604	21.37625130356263
28	21.153538308033987	25.51625742700438	30.325956710064357	23.00424755489728
29	22.760708640735807	25.581066442451306	28.636259789347385	23.021965127465503
30	22.65284903229416	26.20009356121654	29.115100500599134	22.03195690589016
31	23.203337120334954	26.728201557592214	26.994586815328496	23.073874506744335
32	22.49276920996722	27.716500188832022	27.208130189966568	22.582600411234186
33	21.90886640173197	26.810883562910593	27.721784377141844	23.558465658215592
34	21.200008081699767	26.01545780497926	28.387592104179326	24.396942009141647
35	22.505047176922396	26.59454267681438	27.466122913328434	23.434287232934793
36	22.345433606505146	27.717898944561092	26.976092156244125	22.960575292689636
37	21.515971459166437	27.94993697828354	27.115812311847925	23.4182792507021
38	20.525807820287863	28.89922586641261	27.231131950844617	23.34383436245491
39	21.731379841443268	25.795542320908755	28.23745898925911	24.235618848388864
40	21.515660624559974	25.5893035595225	29.876645286426317	23.01839052949121
41	20.75007498884881	26.50688731779264	28.123538105991493	24.61949958736706
42	21.08733053685799	27.138036986209823	27.77866711012404	23.995965366808147
43	21.439816980583718	25.380267286678095	28.9436752151364	24.236240517601786
44	21.770855836463696	25.335662520651077	28.676046618974276	24.21743502391095
45	22.080136269891472	25.029179598681438	28.16689953359267	24.723784597834413
46	21.970567071114296	26.00457859375316	28.38153082935336	23.643323505779193
47	21.432356950028677	25.545631297314852	29.540322242236517	23.481689510419955
48	20.38981767996158	25.840147086935776	29.087591637927417	24.682443595175226
49	21.257357066591652	25.232776265912786	29.836081370283278	23.67378529721228
50	20.41950238487852	24.538060920474518	30.693363214900167	24.349073479746792
51	20.7898618184757	24.728447116931314	28.391322119456845	26.090368945136138
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	363.0
1	889.5
2	1416.0
3	5980.5
4	10545.0
5	6692.0
6	2839.0
7	2714.5
8	2590.0
9	2497.0
10	2404.0
11	2399.5
12	2395.0
13	2459.5
14	2524.0
15	2378.0
16	2232.0
17	2184.0
18	2136.0
19	2068.0
20	2000.0
21	2107.0
22	2214.0
23	2453.0
24	2692.0
25	3061.0
26	4597.5
27	5765.0
28	6822.5
29	7880.0
30	9350.0
31	10820.0
32	12225.5
33	13631.0
34	14540.5
35	15450.0
36	16492.0
37	17534.0
38	19643.0
39	21752.0
40	24762.0
41	27772.0
42	30293.0
43	32814.0
44	36199.0
45	39584.0
46	44673.0
47	49762.0
48	54330.0
49	58898.0
50	59041.0
51	59184.0
52	52725.0
53	46266.0
54	41834.0
55	37402.0
56	34904.0
57	32406.0
58	30679.0
59	28952.0
60	27280.0
61	25608.0
62	24115.0
63	22622.0
64	19946.5
65	17271.0
66	14268.0
67	11265.0
68	9531.5
69	7798.0
70	6520.5
71	5243.0
72	4298.0
73	3353.0
74	2698.0
75	1633.5
76	1224.0
77	930.5
78	637.0
79	506.5
80	376.0
81	287.5
82	199.0
83	142.0
84	85.0
85	56.0
86	27.0
87	18.5
88	10.0
89	13.0
90	16.0
91	8.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	643429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.79324270546256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36881186755267	23.73208011878614
2	8.875194002120924	5.820927819392342
3	3.435976317446944	3.3803041592477734
4	2.033911146268328	2.6679416744369133
5	1.4403890308134595	2.361751353887588
6	1.174221734086542	2.3103922989557457
7	1.0067323537997588	2.3109812892317962
8	0.8936450280941113	2.344441463905608
9	0.7945726055931764	2.345095107209593
>10	7.854033637400424	45.777868824313245
>50	0.0734120258022255	1.6357938911831533
>100	0.04385653489295694	2.9755065867502157
>500	0.003813611729822343	0.8633877733855964
>1k	9.534029324555857E-4	0.36674048820615773
>5k	4.7670146622779287E-4	1.1067871511080976
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7080	1.1003545068686678	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1275	0.1981570616182982	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1071	0.1664519317593705	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCC	885	0.13754431335858347	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG	766	0.11904965427420897	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	755	0.11734006393867855	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	741	0.11516422169345801	TruSeq Adapter, Index 13 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	661	0.10273083743505498	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1083460646007564E-4	0.0	0.0	0.11936048888066904	0.0
2	4.6625190969011343E-4	0.0	0.0	0.5968024444033452	0.0
3	4.6625190969011343E-4	0.0	0.0	0.9051503740117403	0.0
4	4.6625190969011343E-4	0.0	0.0	1.2100791229490744	0.0
5	4.6625190969011343E-4	0.0	0.0	2.174443489491459	0.0
6	4.6625190969011343E-4	0.0	0.0	2.881592219188131	0.0
7	4.6625190969011343E-4	0.0	0.0	3.5355882311801303	0.0
8	4.6625190969011343E-4	0.0	0.0	4.496844251657914	0.0
9	4.6625190969011343E-4	0.0	0.0	4.895489634442961	0.0
10	4.6625190969011343E-4	0.0	0.0	5.685475786761243	0.0
11	4.6625190969011343E-4	0.0	0.0	6.78272194756531	0.0
12	4.6625190969011343E-4	0.0	0.0	7.619177873549374	0.0
13	4.6625190969011343E-4	0.0	0.0	7.967312632784658	0.0
14	4.6625190969011343E-4	0.0	0.0	8.114492818943503	0.0
15	4.6625190969011343E-4	0.0	0.0	8.395953555093103	0.0
16	4.6625190969011343E-4	0.0	0.0	8.936339518423944	0.0
17	4.6625190969011343E-4	0.0	0.0	9.556765392918255	0.0
18	4.6625190969011343E-4	0.0	0.0	10.226613969839718	0.0
19	4.6625190969011343E-4	0.0	0.0	10.611738047243751	0.0
20	4.6625190969011343E-4	0.0	0.0	11.006653414751279	0.0
21	4.6625190969011343E-4	0.0	0.0	11.496373337229127	0.0
22	4.6625190969011343E-4	0.0	0.0	12.019663397204665	0.0
23	4.6625190969011343E-4	0.0	0.0	12.565644383451787	0.0
24	4.6625190969011343E-4	0.0	0.0	13.010914957205845	0.0
25	4.6625190969011343E-4	0.0	0.0	13.429298337501107	0.0
26	4.6625190969011343E-4	0.0	0.0	13.807739470866249	0.0
27	4.6625190969011343E-4	0.0	0.0	14.187268525354002	0.0
28	4.6625190969011343E-4	0.0	0.0	14.580318885222768	0.0
29	4.6625190969011343E-4	0.0	0.0	14.979896771827194	0.0
30	4.6625190969011343E-4	0.0	0.0	15.417551897722982	0.0
31	6.216692129201513E-4	0.0	0.0	15.830029420495501	0.0
32	6.216692129201513E-4	0.0	0.0	16.23706733765497	0.0
33	6.216692129201513E-4	0.0	0.0	16.63835481459493	0.0
34	6.216692129201513E-4	0.0	0.0	17.033736434012145	0.0
35	6.216692129201513E-4	0.0	0.0	17.456160664191387	0.0
36	6.216692129201513E-4	0.0	0.0	17.8515422836086	0.0
37	6.216692129201513E-4	0.0	0.0	18.238375951348168	0.0
38	6.216692129201513E-4	0.0	0.0	18.644636781991487	0.0
39	6.216692129201513E-4	0.0	0.0	19.06861518520303	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	20	7.030968E-4	45.000004	1
CGGTCTA	30	2.1640062E-6	45.000004	31
GATTCGT	20	7.030968E-4	45.000004	11
CGTTTAT	20	7.030968E-4	45.000004	39
GTTAGCG	25	3.888884E-5	45.0	1
GCGTAAG	50	2.1827873E-11	45.0	1
CGTTTTT	2800	0.0	41.785713	1
TCGGCGT	145	0.0	40.344826	4
GCTAGCG	45	1.925946E-8	40.0	1
TAGTGCG	40	3.4550612E-7	39.375004	1
CGTTTGG	40	3.4550612E-7	39.375004	2
AATGGGC	155	0.0	39.193546	4
CGACGGT	35	6.2440013E-6	38.57143	28
CACGACG	35	6.2440013E-6	38.57143	26
AAATGCG	35	6.2440013E-6	38.57143	1
ACGGTCT	35	6.2440013E-6	38.57143	30
CGTAAGG	100	0.0	38.25	2
ATGGGCG	155	0.0	37.741936	5
CGTTCAT	60	1.546141E-10	37.500004	17
TACGAAT	60	1.546141E-10	37.500004	12
>>END_MODULE