FastQCFastQC Report
Sat 18 Jun 2016
SRR3551533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631962
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76631.2125729078647134No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC14420.22817827654194398RNA PCR Primer, Index 48 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT9370.14826840854355167TruSeq Adapter, Index 4 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC9190.1454201360208367RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC8760.1386159294387954No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC7980.12627341517369714No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC7790.12326690528860912No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC7100.1123485272848684RNA PCR Primer, Index 4 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6900.10918378003740731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGAA207.030873E-445.09
ATAACGC207.030873E-445.011
TTCGCTA207.030873E-445.014
CGTTTTT29650.041.964591
CGGGTAT700.041.7857136
CGAATAT603.6379788E-1241.24999614
GCTACGA603.6379788E-1241.24999610
ATTAGTC1150.041.08695631
TCACGAC556.002665E-1140.90909225
TACGAAA900.040.020
CATACGA900.040.018
AGTACGG451.925946E-840.02
TCGATAG356.2438266E-638.5714261
AACGAGC700.038.57142615
TAGTACG356.2438266E-638.5714261
CTACGAA659.094947E-1238.0769211
TAGGTCG301.1393406E-437.4999961
CACGACG601.546141E-1037.49999626
TCGTAAG301.1393406E-437.4999961
GATTACG301.1393406E-437.4999961
Produced by FastQC (version 0.11.5)