SRR3551531_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525021
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4463	0.8500612356458123	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1763	0.3357960919658452	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	1073	0.20437277746985358	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	963	0.18342123457918824	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	854	0.16266016026025626	Illumina PCR Primer Index 8 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	561	0.10685286874239316	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAC	20	7.0297817E-4	45.0	33
AATACGG	20	7.0297817E-4	45.0	2
TCGAAGG	35	1.2102464E-7	45.0	2
GTACGAG	40	6.8030204E-9	45.0	1
CGTTTTT	2125	0.0	43.411762	1
CGTAAGG	85	0.0	42.35294	2
CCGGGTA	40	3.4534423E-7	39.375	5
ACGTTAG	35	6.2418057E-6	38.571426	1
AGCGACT	165	0.0	38.181816	19
TAGGGTA	250	0.0	37.8	5
AAGTACG	30	1.13907714E-4	37.500004	1
TAGCGAC	30	1.13907714E-4	37.500004	30
CATACGA	60	1.546141E-10	37.500004	18
TATAGCG	30	1.13907714E-4	37.500004	1
TGCGTAG	60	1.546141E-10	37.500004	1
CGAATAT	55	2.743036E-9	36.81818	14
GGTACTA	25	0.0021060687	36.0	8
ACCGGGT	50	4.870344E-8	36.0	4
CGTATGG	50	4.870344E-8	36.0	2
TAGACGG	50	4.870344E-8	36.0	2