##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	525021
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39350616451532	33.0	31.0	34.0	31.0	34.0
2	32.52439807169618	34.0	31.0	34.0	31.0	34.0
3	32.52225339557846	34.0	31.0	34.0	31.0	34.0
4	36.0232295470086	37.0	35.0	37.0	35.0	37.0
5	36.115564901213475	37.0	35.0	37.0	35.0	37.0
6	36.14262477119963	37.0	36.0	37.0	35.0	37.0
7	36.27610705095606	37.0	37.0	37.0	35.0	37.0
8	36.20226429038077	37.0	37.0	37.0	35.0	37.0
9	38.07722738709499	39.0	39.0	39.0	37.0	39.0
10	37.68940861413162	39.0	37.0	39.0	35.0	39.0
11	37.7102839695936	39.0	37.0	39.0	35.0	39.0
12	37.67450825776493	39.0	37.0	39.0	35.0	39.0
13	37.67080935810186	39.0	37.0	39.0	35.0	39.0
14	39.01016911704484	40.0	38.0	41.0	35.0	41.0
15	39.074846529948324	40.0	38.0	41.0	35.0	41.0
16	39.08744221659705	40.0	38.0	41.0	35.0	41.0
17	39.03234537285175	40.0	38.0	41.0	35.0	41.0
18	38.94843634826036	40.0	38.0	41.0	35.0	41.0
19	38.85231066947798	40.0	38.0	41.0	35.0	41.0
20	38.75589357378086	40.0	38.0	41.0	35.0	41.0
21	38.66232779260258	40.0	38.0	41.0	35.0	41.0
22	38.67512537593734	40.0	38.0	41.0	35.0	41.0
23	38.63015193677967	40.0	38.0	41.0	35.0	41.0
24	38.55470352614467	40.0	38.0	41.0	35.0	41.0
25	38.45008104437727	40.0	37.0	41.0	35.0	41.0
26	38.47577906407553	40.0	37.0	41.0	35.0	41.0
27	38.485167259976265	40.0	38.0	41.0	35.0	41.0
28	38.42658865073968	40.0	37.0	41.0	35.0	41.0
29	38.387561640391525	40.0	37.0	41.0	35.0	41.0
30	38.23432967443207	40.0	37.0	41.0	34.0	41.0
31	38.17466539433661	40.0	37.0	41.0	34.0	41.0
32	38.04358873264117	40.0	37.0	41.0	34.0	41.0
33	37.90416573813238	40.0	37.0	41.0	34.0	41.0
34	37.73994373558391	40.0	37.0	41.0	33.0	41.0
35	37.66168781820156	40.0	37.0	41.0	33.0	41.0
36	37.57888351132621	40.0	37.0	41.0	33.0	41.0
37	37.5398393588066	40.0	36.0	41.0	33.0	41.0
38	37.40727894693736	40.0	36.0	41.0	33.0	41.0
39	37.37698872997461	40.0	36.0	41.0	33.0	41.0
40	37.31259321055729	40.0	36.0	41.0	33.0	41.0
41	37.24759390576758	40.0	36.0	41.0	33.0	41.0
42	37.18673729241306	40.0	35.0	41.0	33.0	41.0
43	37.18240603709185	40.0	35.0	41.0	33.0	41.0
44	37.12381790442668	40.0	35.0	41.0	33.0	41.0
45	36.99968001279949	40.0	35.0	41.0	32.0	41.0
46	36.95301711741054	39.0	35.0	41.0	32.0	41.0
47	36.91225493837389	39.0	35.0	41.0	32.0	41.0
48	36.86853287773251	39.0	35.0	41.0	32.0	41.0
49	36.83474375310702	39.0	35.0	41.0	32.0	41.0
50	36.757518270697744	39.0	35.0	41.0	32.0	41.0
51	35.98979278924081	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	9.0
10	10.0
11	12.0
12	11.0
13	8.0
14	8.0
15	21.0
16	42.0
17	64.0
18	83.0
19	149.0
20	250.0
21	419.0
22	614.0
23	1015.0
24	1584.0
25	2601.0
26	4046.0
27	5163.0
28	5186.0
29	5249.0
30	5463.0
31	6378.0
32	8098.0
33	11534.0
34	23147.0
35	33869.0
36	32873.0
37	49181.0
38	99953.0
39	227702.0
40	274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.14002868456691	20.10300540359338	24.630443353694425	13.126522558145293
2	34.726801404134314	22.669759876271616	24.70205953666615	17.901379182927922
3	32.370895640364864	23.10002837981719	27.973928661901144	16.555147317916806
4	27.85736189600035	24.45692648484537	28.392769051142718	19.292942568011565
5	24.155224267219786	29.272733852550658	26.65817176836736	19.91387011186219
6	25.16604097740852	33.18248222452054	26.525415173869238	15.126061624201698
7	77.31290748370066	6.092137266890277	11.4700173897806	5.1249378596284725
8	77.78546001017102	5.478447528765516	11.373068886768339	5.3630235742951236
9	71.60761188600075	7.471510663382988	13.715641850516455	7.205235600099806
10	35.210972513480414	24.068561067081127	23.57448559200489	17.14598082743357
11	28.516573622769375	25.183754554579725	26.16504863614979	20.13462318650111
12	25.14299428022879	22.65280817338735	31.23798857569507	20.96620897068879
13	23.16897800278465	23.863235946752607	32.50974722915846	20.458038821304292
14	20.963542410684525	25.83134769847301	31.455884621757985	21.749225269084473
15	20.081482454987515	25.015189868595733	33.91483388283516	20.98849379358159
16	22.08959260677192	24.651775833728557	32.14804741143688	21.11058414806265
17	22.443864150195896	24.015229866995796	29.959944459364486	23.580961523443825
18	23.001365659659328	24.222269204469917	30.981808346713752	21.794556789157006
19	22.98193786534253	25.253846988977585	29.85671049348502	21.907504652194866
20	23.857521889600605	25.5060273779525	29.44415556711065	21.192295165336244
21	23.003460813948394	26.374183127912982	30.07841591098261	20.543940147156018
22	22.385771235817234	24.37235843899577	29.18588018384027	24.05599014134673
23	21.482378800086092	25.99572207587887	30.151174905384735	22.370724218650302
24	21.899504972182065	24.850815491189877	30.619537123276974	22.630142413351084
25	21.63094428603808	26.025435173069265	29.408918881339986	22.934701659552665
26	20.854213450509597	26.00657878446767	29.921088870730884	23.218118894291848
27	22.182350801206045	25.014047057165335	29.750809967601295	23.052792174027324
28	19.922822134733657	26.088099333169527	30.476876163048715	23.512202369048097
29	21.261054319731972	25.669449412499688	30.004704573721813	23.064791694046523
30	22.025023760954323	25.164898165978123	29.718620778978366	23.09145729408919
31	21.783700080568206	28.00230847908941	27.441949941049977	22.77204149929241
32	21.77950977199007	26.503511288119903	28.518478308486706	23.198500631403316
33	21.53323390873889	26.662933482660694	27.217006557832924	24.586826050767492
34	20.700314844549077	27.307098192262785	29.075979817950138	22.916607145238
35	21.271911028320773	25.666020978208493	28.1291605478638	24.93290744560694
36	20.500894249944288	28.259631519501127	27.008253003213206	24.231221227341383
37	20.63346037587068	28.46343289125578	27.34747752947025	23.555629203403292
38	20.960113976393323	28.208014536561393	26.85759236297215	23.974279124073135
39	22.198159692659914	26.697789231287892	27.23910091215399	23.864950163898204
40	21.559137634494622	25.783921024111418	29.253115589662126	23.403825751731837
41	19.506267368352884	26.366754853615383	28.347627999641915	25.779349778389815
42	20.626793975860014	25.691734235392488	28.369912822534715	25.311558966212782
43	21.617992423160217	25.29898804047838	28.211062033709126	24.871957502652275
44	21.874363120713266	25.75935057835782	28.03221204485154	24.334074256077376
45	22.19568360122738	25.028522668617065	27.811839907356084	24.96395382279947
46	21.845983303525003	25.728875606880486	28.416387154037647	24.008753935556864
47	20.417659484096827	26.047148590246866	29.410442629913852	24.124749295742458
48	20.259951506701636	25.262418074705582	29.439774789960783	25.037855628632
49	21.601612125991153	24.33026488464271	29.353682995537323	24.71443999382882
50	20.439753838417893	24.056371078490194	30.12908055106367	25.374794532028243
51	20.22433388378751	24.15065302149819	28.25925058235766	27.36576251235665
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	655.5
2	1017.0
3	4110.5
4	7204.0
5	4683.0
6	2162.0
7	2095.0
8	2028.0
9	1996.5
10	1965.0
11	1877.5
12	1790.0
13	1780.0
14	1770.0
15	1764.0
16	1758.0
17	1729.0
18	1700.0
19	1688.5
20	1677.0
21	1840.5
22	2004.0
23	2111.0
24	2218.0
25	2474.5
26	3536.5
27	4342.0
28	4853.0
29	5364.0
30	6518.0
31	7672.0
32	8803.5
33	9935.0
34	11148.5
35	12362.0
36	13124.5
37	13887.0
38	15701.5
39	17516.0
40	20424.0
41	23332.0
42	24882.0
43	26432.0
44	29832.5
45	33233.0
46	38789.0
47	44345.0
48	49164.5
49	53984.0
50	53287.5
51	52591.0
52	46690.0
53	40789.0
54	36245.0
55	31701.0
56	29104.5
57	26508.0
58	24275.5
59	22043.0
60	20234.5
61	18426.0
62	17122.0
63	15818.0
64	13594.5
65	11371.0
66	9725.5
67	8080.0
68	6503.5
69	4927.0
70	4259.0
71	3591.0
72	3083.0
73	2575.0
74	2162.5
75	1391.5
76	1033.0
77	810.5
78	588.0
79	429.5
80	271.0
81	197.0
82	123.0
83	105.5
84	88.0
85	50.5
86	13.0
87	8.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	525021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.14307965783613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.3900289704275	26.163985835112264
2	9.36494608448662	6.76955984645881
3	3.4743235489221846	3.7671825835737125
4	2.13992878090398	3.093744655612348
5	1.584039999904917	2.862604194888108
6	1.2867892628147395	2.790511609725682
7	1.0986302042178326	2.779551528988493
8	0.9449851541785363	2.7323738962357917
9	0.8592021983279938	2.7948792146720938
>10	6.752752171656892	40.40185795442306
>50	0.06516728818035576	1.6184868711204294
>100	0.03602744387206007	2.3721977472121543
>500	0.0015894460531791206	0.45536698763888456
>1k	0.0015894460531791206	1.3976970743381911
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4463	0.8500612356458123	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1763	0.3357960919658452	RNA PCR Primer, Index 40 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	1073	0.20437277746985358	Illumina PCR Primer Index 8 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCC	963	0.18342123457918824	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	854	0.16266016026025626	Illumina PCR Primer Index 8 (95% over 23bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	561	0.10685286874239316	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.809371434666423E-4	0.0	0.0	0.2106582403370532	0.0
2	3.809371434666423E-4	0.0	0.0	0.8969165042922093	0.0
3	3.809371434666423E-4	0.0	0.0	1.2938530077844506	0.0
4	3.809371434666423E-4	0.0	0.0	1.77154818569162	0.0
5	3.809371434666423E-4	0.0	0.0	3.0892097649427357	0.0
6	3.809371434666423E-4	0.0	0.0	3.979650337796012	0.0
7	3.809371434666423E-4	0.0	0.0	4.805522064831693	0.0
8	3.809371434666423E-4	0.0	0.0	6.021473426777215	0.0
9	3.809371434666423E-4	0.0	0.0	6.5277388904443825	0.0
10	3.809371434666423E-4	0.0	0.0	7.613219280752579	0.0
11	3.809371434666423E-4	0.0	0.0	9.08135103167302	0.0
12	3.809371434666423E-4	0.0	0.0	10.151022530527351	0.0
13	3.809371434666423E-4	0.0	0.0	10.63328895415612	0.0
14	3.809371434666423E-4	0.0	0.0	10.825090805891573	0.0
15	3.809371434666423E-4	0.0	0.0	11.194028429339017	0.0
16	3.809371434666423E-4	0.0	0.0	11.929046647657904	0.0
17	3.809371434666423E-4	0.0	0.0	12.739680888954918	0.0
18	3.809371434666423E-4	0.0	0.0	13.601360707476463	0.0
19	3.809371434666423E-4	0.0	0.0	14.134101302614562	0.0
20	3.809371434666423E-4	0.0	0.0	14.661699246315862	0.0
21	3.809371434666423E-4	0.0	0.0	15.316149258791553	0.0
22	3.809371434666423E-4	0.0	0.0	15.978027545564844	0.0
23	3.809371434666423E-4	0.0	0.0	16.634763180901334	0.0
24	3.809371434666423E-4	0.0	0.0	17.1701703360437	0.0
25	3.809371434666423E-4	0.0	0.0	17.6646267482634	0.0
26	3.809371434666423E-4	0.0	0.0	18.12898912614924	0.0
27	3.809371434666423E-4	0.0	0.0	18.57411417829001	0.0
28	3.809371434666423E-4	0.0	0.0	19.05352357334278	0.0
29	3.809371434666423E-4	0.0	0.0	19.523028602665416	0.0
30	3.809371434666423E-4	0.0	0.0	20.056912009233915	0.0
31	3.809371434666423E-4	0.0	0.0	20.548130455734153	0.0
32	3.809371434666423E-4	0.0	0.0	21.02696844507172	0.0
33	3.809371434666423E-4	0.0	0.0	21.496283005822626	0.0
34	3.809371434666423E-4	0.0	0.0	21.95150289226526	0.0
35	3.809371434666423E-4	0.0	0.0	22.430531350174565	0.0
36	3.809371434666423E-4	0.0	0.0	22.9034648137884	0.0
37	3.809371434666423E-4	0.0	0.0	23.374493591684903	0.0
38	3.809371434666423E-4	0.0	0.0	23.87961624392167	0.0
39	3.809371434666423E-4	0.0	0.0	24.36988234756324	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAC	20	7.0297817E-4	45.0	33
AATACGG	20	7.0297817E-4	45.0	2
TCGAAGG	35	1.2102464E-7	45.0	2
GTACGAG	40	6.8030204E-9	45.0	1
CGTTTTT	2125	0.0	43.411762	1
CGTAAGG	85	0.0	42.35294	2
CCGGGTA	40	3.4534423E-7	39.375	5
ACGTTAG	35	6.2418057E-6	38.571426	1
AGCGACT	165	0.0	38.181816	19
TAGGGTA	250	0.0	37.8	5
AAGTACG	30	1.13907714E-4	37.500004	1
TAGCGAC	30	1.13907714E-4	37.500004	30
CATACGA	60	1.546141E-10	37.500004	18
TATAGCG	30	1.13907714E-4	37.500004	1
TGCGTAG	60	1.546141E-10	37.500004	1
CGAATAT	55	2.743036E-9	36.81818	14
GGTACTA	25	0.0021060687	36.0	8
ACCGGGT	50	4.870344E-8	36.0	4
CGTATGG	50	4.870344E-8	36.0	2
TAGACGG	50	4.870344E-8	36.0	2
>>END_MODULE