FastQCFastQC Report
Sat 18 Jun 2016
SRR3551527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402557
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC19330.4801804464957758TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.46825666924187137No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT12600.3129991529149909TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC12500.31051503265376085TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC7030.1746336543644751TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC5630.13985597070725386No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5190.12892584155784148No Hit
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC4720.11725047633006008TruSeq Adapter, Index 22 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG4470.11104017567698489No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGA351.2093005E-745.00000410
CGACACC207.027819E-445.035
GTACTAG207.027819E-445.01
GTTCCGA207.027819E-445.09
CTCGAGG253.8862745E-545.02
TCGACGG502.1827873E-1145.02
ATCATCG207.027819E-445.037
TCGAAGG207.027819E-445.02
TAGGGCG1150.041.0869565
TCGCGGG556.002665E-1140.9090923
TACGGGT501.0786607E-940.54
CATACGA501.0786607E-940.518
AACGAGC451.9230356E-840.015
CGTTTAC451.9230356E-840.033
TAGGGAT1650.039.5454525
AGGGTAC1200.039.3750046
CTACGAA403.4507684E-739.37511
AAGCTAC403.4507684E-739.3758
CGTTTTT10250.039.292681
TACACGG356.238177E-638.571432
Produced by FastQC (version 0.11.5)