##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551527_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	402557
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.276904885519315	33.0	31.0	34.0	30.0	34.0
2	32.37991141627148	34.0	31.0	34.0	30.0	34.0
3	32.40918428942982	34.0	31.0	34.0	30.0	34.0
4	35.93989671027954	37.0	35.0	37.0	35.0	37.0
5	36.03253700718159	37.0	35.0	37.0	35.0	37.0
6	36.05260373065181	37.0	35.0	37.0	35.0	37.0
7	36.18632144019356	37.0	36.0	37.0	35.0	37.0
8	36.08795524608937	37.0	36.0	37.0	35.0	37.0
9	37.925826653119934	39.0	38.0	39.0	35.0	39.0
10	37.60941680308627	39.0	37.0	39.0	35.0	39.0
11	37.607583522333485	39.0	37.0	39.0	35.0	39.0
12	37.52256699051315	39.0	37.0	39.0	35.0	39.0
13	37.511959796997694	39.0	37.0	39.0	35.0	39.0
14	38.78504907379576	40.0	38.0	41.0	35.0	41.0
15	38.84429782614636	40.0	38.0	41.0	35.0	41.0
16	38.852803453920814	40.0	38.0	41.0	35.0	41.0
17	38.81846297542957	40.0	38.0	41.0	35.0	41.0
18	38.757708846200664	40.0	38.0	41.0	35.0	41.0
19	38.68949490382728	40.0	38.0	41.0	35.0	41.0
20	38.64452487473824	40.0	38.0	41.0	35.0	41.0
21	38.52163296129492	40.0	38.0	41.0	35.0	41.0
22	38.52097218530543	40.0	38.0	41.0	35.0	41.0
23	38.47649152790785	40.0	37.0	41.0	35.0	41.0
24	38.40387324031131	40.0	37.0	41.0	34.0	41.0
25	38.273727695705205	40.0	37.0	41.0	34.0	41.0
26	38.29007569114436	40.0	37.0	41.0	34.0	41.0
27	38.30858983945131	40.0	37.0	41.0	34.0	41.0
28	38.26195296566697	40.0	37.0	41.0	34.0	41.0
29	38.22504887506614	40.0	37.0	41.0	34.0	41.0
30	38.072727588888036	40.0	37.0	41.0	34.0	41.0
31	38.037467985900136	40.0	37.0	41.0	34.0	41.0
32	37.964688230486615	40.0	37.0	41.0	34.0	41.0
33	37.90644306272155	40.0	37.0	41.0	34.0	41.0
34	37.781062060776485	40.0	37.0	41.0	33.0	41.0
35	37.71634327561066	40.0	36.0	41.0	33.0	41.0
36	37.64195877850838	40.0	36.0	41.0	33.0	41.0
37	37.566525982655875	40.0	36.0	41.0	33.0	41.0
38	37.42586515698398	40.0	36.0	41.0	33.0	41.0
39	37.4091793211893	40.0	35.0	41.0	33.0	41.0
40	37.32172586739269	40.0	35.0	41.0	33.0	41.0
41	37.25230961081288	40.0	35.0	41.0	33.0	41.0
42	37.20804258775776	40.0	35.0	41.0	33.0	41.0
43	37.20128578064721	39.0	35.0	41.0	33.0	41.0
44	37.14357966697884	39.0	35.0	41.0	33.0	41.0
45	37.01621633706531	39.0	35.0	41.0	32.0	41.0
46	36.96022426637718	39.0	35.0	41.0	32.0	41.0
47	36.90085379213379	39.0	35.0	41.0	32.0	41.0
48	36.86388759852642	39.0	35.0	41.0	32.0	41.0
49	36.815265912653366	39.0	35.0	41.0	32.0	41.0
50	36.74346241650251	39.0	35.0	41.0	32.0	41.0
51	35.90092831574162	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	8.0
11	12.0
12	5.0
13	10.0
14	17.0
15	14.0
16	19.0
17	47.0
18	82.0
19	128.0
20	223.0
21	314.0
22	458.0
23	678.0
24	1122.0
25	1772.0
26	2454.0
27	3141.0
28	3464.0
29	3811.0
30	4607.0
31	5360.0
32	7144.0
33	10241.0
34	20448.0
35	28913.0
36	26753.0
37	41846.0
38	80292.0
39	159013.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.545137707206685	20.424436787833773	23.316946420010083	12.71347908494946
2	33.22411484584792	24.21073289000067	24.410704571029694	18.15444769312172
3	33.20349664767971	23.294092513606767	26.935812816570078	16.56659802214345
4	30.152996966889162	24.554286722128793	25.628668735110804	19.664047575871244
5	24.910012743536942	29.392110930874388	25.103525711886764	20.594350613701913
6	25.616496545830774	34.16062818433166	24.753513167079443	15.469362102758119
7	77.13938647197787	6.920013811708653	10.432808273114118	5.507791443199348
8	76.20386678159863	6.371271646002927	11.035207436462414	6.389654135936029
9	68.99892437592689	8.96668049493612	13.62167345245518	8.412721676681812
10	37.59393079737776	23.714902485859145	22.10941556102614	16.581751155736953
11	29.660395919087236	24.142916406869087	25.014345794508603	21.182341879535073
12	26.05668265612075	21.6652548583182	31.018464465901722	21.25959801965933
13	23.781973732912356	23.254346589427087	31.90827634347434	21.055403334186217
14	20.612236279582767	26.84290671880007	30.31794255223484	22.226914449382324
15	20.255268198044003	24.94801978353376	33.45811897445579	21.338593043966444
16	22.035140365215362	23.935243953030255	31.343138984044494	22.68647669770989
17	22.03911495763333	24.1322346897458	28.196503849144346	25.632146503476527
18	22.201576422717775	24.51603127010585	30.772536560039942	22.50985574713643
19	23.485121361695363	24.88343265674178	28.726615112890848	22.904830868672015
20	24.397041909592925	25.24685945095974	28.342570120504675	22.01352851894266
21	23.895746440876696	25.370071815916752	28.90721065588227	21.826971087324278
22	21.956145340908247	24.4474695508959	27.59584356997891	26.000541538216947
23	21.891309802090138	25.260770524422625	28.857776662683793	23.99014301080344
24	23.58150522783109	24.328480190382976	28.574586952903562	23.515427628882367
25	22.530970769356887	25.386218597614747	27.408540902282162	24.674269730746204
26	21.920125597120407	25.5541451272739	27.731724948268194	24.794004327337497
27	23.43941354888873	25.206119878675565	27.85866349361705	23.495803078818653
28	21.148806256008466	24.067399150927695	29.175247232069	25.608547360994837
29	23.4433881413067	24.018958805833705	27.8214016896986	24.716251363160993
30	24.3287286024091	24.26116053130364	26.995183290813472	24.414927575473783
31	24.03311829132272	24.69588157701891	25.552654655117163	25.71834547654121
32	23.686335102855	24.892127077656085	27.68874966774891	23.732788151740003
33	23.33855826628279	24.058456317987268	27.1228670722407	25.480118343489245
34	22.17549315997486	22.898372155992817	29.670829224184402	25.25530545984792
35	23.08045817114098	22.2018248347439	29.81093360691778	24.90678338719734
36	23.918103523227767	23.878854423100332	28.771080865566862	23.43196118810504
37	21.786231515040104	24.39306731717496	30.35992418464963	23.460776983135307
38	21.86969795581744	25.442856539570798	28.366914499064727	24.320531005547043
39	23.784706265199713	22.347642694078107	30.753408834028473	23.11424220669371
40	23.55765767332328	22.1079250888694	32.1032797839809	22.231137453826413
41	20.544419796451187	23.658016131876977	30.04593138363014	25.751632688041692
42	21.696057949557453	23.368616121443672	30.074747178660417	24.860578750338462
43	22.89613644775771	22.14916148520582	29.98134425683816	24.97335781019831
44	22.969417995464	22.57692699418964	28.242708486003227	26.210946524343136
45	23.383520843011052	22.04656731841702	28.099623158956373	26.470288679615557
46	24.053984901517055	22.87477301351113	29.40155058786706	23.669691497104758
47	21.54676232185753	22.721005969340986	31.96839205379611	23.763839655005377
48	20.508896876715596	22.684489401500905	31.022687470345815	25.783926251437684
49	21.72984198511018	21.794429111902165	31.930633425825413	24.545095477162242
50	21.483914079248404	20.92101242805367	31.754260887278075	25.840812605419856
51	21.554959918719586	21.573839232704934	28.745991250928437	28.125209597647043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	479.0
2	814.0
3	1918.0
4	3022.0
5	2034.5
6	1047.0
7	1005.0
8	963.0
9	992.5
10	1022.0
11	1025.0
12	1028.0
13	975.5
14	923.0
15	933.0
16	943.0
17	932.0
18	921.0
19	982.5
20	1044.0
21	1049.5
22	1055.0
23	1151.0
24	1247.0
25	1573.5
26	2487.0
27	3074.0
28	3685.5
29	4297.0
30	4942.5
31	5588.0
32	6367.0
33	7146.0
34	7553.0
35	7960.0
36	8269.5
37	8579.0
38	9736.5
39	10894.0
40	13149.0
41	15404.0
42	17420.5
43	19437.0
44	21785.0
45	24133.0
46	28398.0
47	32663.0
48	38125.0
49	43587.0
50	43131.5
51	42676.0
52	36946.5
53	31217.0
54	27811.0
55	24405.0
56	22450.5
57	20496.0
58	19368.5
59	18241.0
60	17745.0
61	17249.0
62	16033.5
63	14818.0
64	13020.5
65	11223.0
66	9555.0
67	7887.0
68	6791.5
69	5696.0
70	4714.0
71	3732.0
72	3193.0
73	2654.0
74	2106.0
75	1228.0
76	898.0
77	739.0
78	580.0
79	415.5
80	251.0
81	168.5
82	86.0
83	61.5
84	37.0
85	22.5
86	8.0
87	6.0
88	4.0
89	2.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	402557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.543429939502104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.34797002871953	25.71495003980991
2	9.690314441467848	6.888540248841159
3	3.5820502360382944	3.8195505481321232
4	1.998281447977606	2.8410310658239544
5	1.5365917290634377	2.7307870233792353
6	1.1710427776027357	2.4973726153129663
7	0.9882714570859708	2.4588590107301402
8	0.8844262552507677	2.51484341121295
9	0.7881581468003739	2.5212459484842213
>10	6.8805104500373515	40.86168856425385
>50	0.07985008156082225	1.9493580223183693
>100	0.047629873211718535	3.1819597552044803
>500	0.002101317935811112	0.44439387865112256
>1k	0.0028017572477481495	1.5754198678455484
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1933	0.4801804464957758	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1885	0.46825666924187137	No Hit
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	1260	0.3129991529149909	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1250	0.31051503265376085	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	703	0.1746336543644751	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCC	563	0.13985597070725386	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	519	0.12892584155784148	No Hit
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	472	0.11725047633006008	TruSeq Adapter, Index 22 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	447	0.11104017567698489	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32268722193378824	0.0
2	0.0	0.0	0.0	1.428617562233423	0.0
3	0.0	0.0	0.0	1.9559962936925703	0.0
4	0.0	0.0	0.0	2.5626184614849574	0.0
5	0.0	0.0	0.0	4.309451829181954	0.0
6	0.0	0.0	0.0	5.289685684263347	0.0
7	0.0	0.0	0.0	6.278117136206798	0.0
8	2.4841202612300864E-4	0.0	0.0	7.7574107517693145	0.0
9	2.4841202612300864E-4	0.0	0.0	8.345898841654723	0.0
10	2.4841202612300864E-4	0.0	0.0	9.60485099004613	0.0
11	2.4841202612300864E-4	0.0	0.0	11.229465640890608	0.0
12	2.4841202612300864E-4	0.0	0.0	12.446932980919472	0.0
13	2.4841202612300864E-4	0.0	0.0	12.968349823751668	0.0
14	2.4841202612300864E-4	0.0	0.0	13.17751274974724	0.0
15	2.4841202612300864E-4	0.0	0.0	13.605526670757184	0.0
16	2.4841202612300864E-4	0.0	0.0	14.460064040620335	0.0
17	2.4841202612300864E-4	0.0	0.0	15.41247574877595	0.0
18	2.4841202612300864E-4	0.0	0.0	16.45853879077994	0.0
19	2.4841202612300864E-4	0.0	0.0	17.051746709161684	0.0
20	2.4841202612300864E-4	0.0	0.0	17.611419004016824	0.0
21	2.4841202612300864E-4	0.0	0.0	18.34348924500133	0.0
22	2.4841202612300864E-4	0.0	0.0	19.06686506507153	0.0
23	2.4841202612300864E-4	0.0	0.0	19.748259252726942	0.0
24	2.4841202612300864E-4	0.0	0.0	20.33923146287358	0.0
25	2.4841202612300864E-4	0.0	0.0	20.861393541784146	0.0
26	2.4841202612300864E-4	0.0	0.0	21.34480334461952	0.0
27	2.4841202612300864E-4	0.0	0.0	21.83119409176837	0.0
28	2.4841202612300864E-4	0.0	0.0	22.323049903491928	0.0
29	2.4841202612300864E-4	0.0	0.0	22.819625543711823	0.0
30	2.4841202612300864E-4	0.0	0.0	23.33806144223054	0.0
31	2.4841202612300864E-4	0.0	0.0	23.8515291002268	0.0
32	2.4841202612300864E-4	0.0	0.0	24.34760791639445	0.0
33	2.4841202612300864E-4	0.0	0.0	24.82580106668124	0.0
34	2.4841202612300864E-4	0.0	0.0	25.335790956311776	0.0
35	2.4841202612300864E-4	0.0	0.0	25.839818957315362	0.0
36	2.4841202612300864E-4	0.0	0.0	26.31925416773277	0.0
37	2.4841202612300864E-4	0.0	0.0	26.808874271221217	0.0
38	2.4841202612300864E-4	0.0	0.0	27.279118236672073	0.0
39	2.4841202612300864E-4	0.0	0.0	27.74215825336536	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTACGA	35	1.2093005E-7	45.000004	10
CGACACC	20	7.027819E-4	45.0	35
GTACTAG	20	7.027819E-4	45.0	1
GTTCCGA	20	7.027819E-4	45.0	9
CTCGAGG	25	3.8862745E-5	45.0	2
TCGACGG	50	2.1827873E-11	45.0	2
ATCATCG	20	7.027819E-4	45.0	37
TCGAAGG	20	7.027819E-4	45.0	2
TAGGGCG	115	0.0	41.086956	5
TCGCGGG	55	6.002665E-11	40.909092	3
TACGGGT	50	1.0786607E-9	40.5	4
CATACGA	50	1.0786607E-9	40.5	18
AACGAGC	45	1.9230356E-8	40.0	15
CGTTTAC	45	1.9230356E-8	40.0	33
TAGGGAT	165	0.0	39.545452	5
AGGGTAC	120	0.0	39.375004	6
CTACGAA	40	3.4507684E-7	39.375	11
AAGCTAC	40	3.4507684E-7	39.375	8
CGTTTTT	1025	0.0	39.29268	1
TACACGG	35	6.238177E-6	38.57143	2
>>END_MODULE