FastQCFastQC Report
Sat 18 Jun 2016
SRR3551525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223717
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13190.5895841621334096No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC8970.4009529897146842No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT4220.18863117241872543Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC3660.16359954764278084No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG3620.16181157444449906No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC3430.1533187017526607No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC3330.14884876875695632No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC3250.14527282236039282No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC2500.11174832489260986No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA2410.1077253851964759No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAG207.0210965E-445.01
GAGTATC253.880704E-545.027
TATGCGG207.0210965E-445.02
GCGTTGA207.0210965E-445.017
GAAAGGT207.0210965E-445.09
GTCCCGA207.0210965E-445.020
GCGTGAC207.0210965E-445.035
AGGGCGT207.0210965E-445.06
TGCGTAG253.880704E-545.01
ACGGGTA302.1576416E-644.9999965
TAGATGG603.6379788E-1241.2499962
ACATACG556.002665E-1140.90909217
CGTTTTT6450.040.8139531
GCGGGAT1050.040.7142875
ATAGCGG501.0732037E-940.52
TACGAAA501.0732037E-940.520
CATACGA501.0732037E-940.518
ATACGAA501.0732037E-940.519
TGACCCA451.9163053E-840.011
ATTAGTC403.4416007E-739.37531