##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223717
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40876196265818	33.0	31.0	34.0	31.0	34.0
2	32.53448329809536	34.0	31.0	34.0	31.0	34.0
3	32.54404001484018	34.0	31.0	34.0	31.0	34.0
4	35.9918826016798	37.0	35.0	37.0	35.0	37.0
5	36.11118064340216	37.0	35.0	37.0	35.0	37.0
6	36.14014580921432	37.0	36.0	37.0	35.0	37.0
7	36.271637828148954	37.0	37.0	37.0	35.0	37.0
8	36.21300124711131	37.0	37.0	37.0	35.0	37.0
9	38.09905371518481	39.0	39.0	39.0	37.0	39.0
10	37.73633206238239	39.0	37.0	39.0	35.0	39.0
11	37.68499488192672	39.0	37.0	39.0	35.0	39.0
12	37.60140266497405	39.0	37.0	39.0	35.0	39.0
13	37.53105932942065	39.0	37.0	39.0	35.0	39.0
14	38.834737637282814	40.0	38.0	41.0	35.0	41.0
15	38.91328776981633	40.0	38.0	41.0	35.0	41.0
16	38.97331450001565	40.0	38.0	41.0	35.0	41.0
17	38.92964772458061	40.0	38.0	41.0	35.0	41.0
18	38.85812432671634	40.0	38.0	41.0	35.0	41.0
19	38.78895211360782	40.0	38.0	41.0	35.0	41.0
20	38.70803738651958	40.0	38.0	41.0	35.0	41.0
21	38.62276447476052	40.0	38.0	41.0	35.0	41.0
22	38.60441986974615	40.0	38.0	41.0	35.0	41.0
23	38.569089519348104	40.0	38.0	41.0	35.0	41.0
24	38.50056097659096	40.0	37.0	41.0	35.0	41.0
25	38.405311174385496	40.0	37.0	41.0	35.0	41.0
26	38.41607030310615	40.0	37.0	41.0	35.0	41.0
27	38.41150203158455	40.0	37.0	41.0	35.0	41.0
28	38.34240133740395	40.0	37.0	41.0	35.0	41.0
29	38.32612631136659	40.0	37.0	41.0	35.0	41.0
30	38.21718063446229	40.0	37.0	41.0	34.0	41.0
31	38.16216916908416	40.0	37.0	41.0	34.0	41.0
32	38.049772703907166	40.0	37.0	41.0	34.0	41.0
33	37.9374656373901	40.0	37.0	41.0	34.0	41.0
34	37.806313333363136	40.0	36.0	41.0	34.0	41.0
35	37.732429810877136	40.0	36.0	41.0	34.0	41.0
36	37.633478904151225	40.0	36.0	41.0	33.0	41.0
37	37.59579736899744	40.0	36.0	41.0	33.0	41.0
38	37.48431724008457	40.0	36.0	41.0	33.0	41.0
39	37.4769016212447	40.0	36.0	41.0	33.0	41.0
40	37.43068251406911	40.0	35.0	41.0	33.0	41.0
41	37.337162575932986	40.0	35.0	41.0	33.0	41.0
42	37.18404949109813	40.0	35.0	41.0	33.0	41.0
43	37.1880322013973	40.0	35.0	41.0	33.0	41.0
44	37.161646186923655	40.0	35.0	41.0	33.0	41.0
45	37.085871882780474	40.0	35.0	41.0	33.0	41.0
46	37.04874461931816	39.0	35.0	41.0	33.0	41.0
47	36.99911048333386	39.0	35.0	41.0	33.0	41.0
48	36.94628034525763	39.0	35.0	41.0	33.0	41.0
49	36.9036461243446	39.0	35.0	41.0	33.0	41.0
50	36.814153595837595	39.0	35.0	41.0	32.0	41.0
51	35.95615442724514	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	5.0
11	3.0
12	4.0
13	4.0
14	7.0
15	8.0
16	8.0
17	16.0
18	51.0
19	65.0
20	108.0
21	199.0
22	303.0
23	430.0
24	672.0
25	1080.0
26	1477.0
27	1846.0
28	1901.0
29	2040.0
30	2305.0
31	2765.0
32	3597.0
33	5270.0
34	10679.0
35	16885.0
36	13996.0
37	20595.0
38	40469.0
39	96777.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61008327485171	20.33149023096144	24.526969340729583	12.531457153457271
2	32.92239749326157	24.315094516733193	25.06827822650938	17.694229763495844
3	32.60458525726699	23.494414818721868	27.10701466585016	16.79398525816098
4	27.928588350460625	25.51169557968326	27.794490360589496	18.765225709266616
5	25.025813863050196	29.8131121014496	25.832636768774837	19.32843726672537
6	24.63514171922563	35.138143279232246	25.319935454167542	14.906779547374585
7	78.33378777652123	6.223934703218799	10.637993536476888	4.804283983783083
8	79.31180911598135	5.995521127138304	9.162021661295297	5.530648095585048
9	73.09681427875397	8.02442371388853	11.919523326345338	6.959238681012171
10	38.937139332281404	26.365452781862803	20.07670405020629	14.620703835649504
11	30.452759513134897	23.971356669363526	25.373574650115994	20.202309167385582
12	27.07036121528538	21.352422927180324	30.85103054305216	20.726185314482134
13	24.859085362310417	24.70844862035518	30.878297134325955	19.554168883008444
14	21.82668281802456	26.959506876991913	29.55877291399402	21.65503739098951
15	20.48838487911066	25.61316305868575	33.62730592668416	20.271146135519427
16	22.687144919697655	23.505589651211128	31.536718264593215	22.270547164498
17	22.880246025112083	23.73310924069248	28.661210368456576	24.725434365738856
18	22.38676542238632	23.927551326005624	30.686537008810237	22.99914624279782
19	24.105007665935087	24.7307982853337	28.521748458990597	22.64244558974061
20	24.571221677387058	25.03207176924418	29.27403818216765	21.12266837120112
21	23.776914584050385	26.29572182712981	29.107756674727447	20.819606914092358
22	22.91734646897643	24.12825131751275	27.713137580067677	25.241264633443144
23	21.96837969398839	25.969863711742963	28.87844911204781	23.18330748222084
24	22.230764760836237	25.00435818467081	30.01694104605372	22.747936008439236
25	21.82847079122284	25.828166835779133	28.693393886025646	23.64996848697238
26	20.75211092585722	27.07706611477894	28.18739747091191	23.98342548845193
27	22.262948278405307	27.218762990742768	28.272773191129865	22.24551553972206
28	19.962720758815824	26.726623367915714	29.83412078652941	23.47653508673905
29	21.98223648627507	25.03251876254375	28.60980613900598	24.375438612175206
30	22.73318522955341	26.83658371961004	27.746215084235885	22.68401596660066
31	22.92941528806483	27.580827563394827	26.391825386537455	23.097931762002887
32	24.724093385840145	26.592972371344153	25.645346576254823	23.037587666560878
33	23.28611594112204	26.89379886195506	25.82369690278343	23.99638829413947
34	21.399357223635217	25.635512723664274	27.914731558173944	25.050398494526565
35	23.66159031276121	24.672689156389545	26.504020704729637	25.161699826119605
36	22.152987926710978	28.384968509322047	25.82637886258085	23.635664701386126
37	21.814167005636588	27.821756951863293	28.28037207722256	22.08370396527756
38	22.688485899596365	27.342580134723782	26.14285011867672	23.826083847003133
39	22.68312198000152	25.932763267878617	27.886123987001433	23.49799076511843
40	22.895890790597047	23.888215915643425	29.323207445120396	23.89268584863913
41	21.176307567149568	25.840235654867534	27.918307504570507	25.06514927341239
42	21.622406880120867	27.295645838268882	26.757912898885643	24.3240343827246
43	23.20118721420366	26.158047890862118	26.66091535287886	23.979849542055366
44	21.783324467966224	26.096362815521395	27.46505629880608	24.655256417706298
45	21.66800019667705	24.98066753979358	27.37074071259672	25.98059155093265
46	23.105083654796015	25.997130303016757	27.496345829776015	23.401440212411213
47	21.247379501781268	26.166987756853526	29.430038843717732	23.155593897647474
48	21.688114895157725	25.173768645208007	27.998766298493184	25.13935016114108
49	22.126168328736753	24.509536602046335	29.716561548742384	23.647733520474528
50	21.30057170443015	23.979402548755793	29.611518123343334	25.10850762347072
51	21.22905277649888	24.23955264910579	27.54238613963177	26.989008434763562
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	109.0
1	302.5
2	496.0
3	1279.5
4	2063.0
5	1361.0
6	659.0
7	629.5
8	600.0
9	634.0
10	668.0
11	666.5
12	665.0
13	644.0
14	623.0
15	600.0
16	577.0
17	571.0
18	565.0
19	593.5
20	622.0
21	627.5
22	633.0
23	766.5
24	900.0
25	1033.0
26	1661.5
27	2157.0
28	2035.5
29	1914.0
30	2553.0
31	3192.0
32	3686.0
33	4180.0
34	4339.0
35	4498.0
36	5274.5
37	6051.0
38	6525.0
39	6999.0
40	8341.5
41	9684.0
42	10922.0
43	12160.0
44	13352.5
45	14545.0
46	16677.5
47	18810.0
48	20460.0
49	22110.0
50	22042.5
51	21975.0
52	19620.0
53	17265.0
54	15421.0
55	13577.0
56	12855.0
57	12133.0
58	10861.5
59	9590.0
60	9025.5
61	8461.0
62	7572.5
63	6684.0
64	5996.0
65	5308.0
66	4558.0
67	3808.0
68	3257.0
69	2706.0
70	2390.0
71	2074.0
72	1787.0
73	1500.0
74	1224.5
75	761.5
76	574.0
77	402.5
78	231.0
79	203.5
80	176.0
81	108.0
82	40.0
83	23.0
84	6.0
85	4.5
86	3.0
87	2.0
88	1.0
89	5.5
90	10.0
91	5.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	223717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.412521176307564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9441082235671	26.56078885377508
2	8.659358466014412	6.30618147033976
3	3.4421379555861087	3.7601076359865364
4	2.13967419992389	3.116437284605104
5	1.5774419660942045	2.8719319497400737
6	1.3159671499245038	2.8750609028370664
7	1.1072783295073716	2.8223156934877545
8	0.9427824357668088	2.74632683256078
9	0.8482586759308136	2.779851330028563
>10	6.935834325628215	41.772864824756276
>50	0.061379064828568274	1.538997930421023
>100	0.023324044634855944	1.8585981396138873
>500	0.0012275812965713653	0.4009529897146842
>1k	0.0012275812965713653	0.5895841621334096
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.5895841621334096	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	897	0.4009529897146842	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	422	0.18863117241872543	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	366	0.16359954764278084	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	362	0.16181157444449906	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	343	0.1533187017526607	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	333	0.14884876875695632	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	325	0.14527282236039282	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	250	0.11174832489260986	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	241	0.1077253851964759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.939865991408789E-4	0.0	0.0	0.20293495800497952	0.0
2	8.939865991408789E-4	0.0	0.0	1.036577461703849	0.0
3	8.939865991408789E-4	0.0	0.0	1.4044529472503207	0.0
4	8.939865991408789E-4	0.0	0.0	1.907320409267065	0.0
5	8.939865991408789E-4	0.0	0.0	3.2290795960968546	0.0
6	8.939865991408789E-4	0.0	0.0	3.9125323511400563	0.0
7	8.939865991408789E-4	0.0	0.0	4.6371084897437385	0.0
8	8.939865991408789E-4	0.0	0.0	5.782305323243205	0.0
9	8.939865991408789E-4	0.0	0.0	6.221699736720947	0.0
10	8.939865991408789E-4	0.0	0.0	7.269899024213627	0.0
11	8.939865991408789E-4	0.0	0.0	8.546064894487232	0.0
12	8.939865991408789E-4	0.0	0.0	9.663101150113759	0.0
13	8.939865991408789E-4	0.0	0.0	10.051091334140901	0.0
14	8.939865991408789E-4	0.0	0.0	10.187871283809455	0.0
15	8.939865991408789E-4	0.0	0.0	10.546359910064949	0.0
16	8.939865991408789E-4	0.0	0.0	11.196735160939937	0.0
17	8.939865991408789E-4	0.0	0.0	11.978526441888636	0.0
18	8.939865991408789E-4	0.0	0.0	12.81619188528364	0.0
19	8.939865991408789E-4	0.0	0.0	13.35571279786516	0.0
20	8.939865991408789E-4	0.0	0.0	13.82282079591627	0.0
21	0.0013409798987113182	0.0	0.0	14.4030180987587	0.0
22	0.0013409798987113182	0.0	0.0	15.011822972773638	0.0
23	0.0013409798987113182	0.0	0.0	15.591573282316498	0.0
24	0.0013409798987113182	0.0	0.0	16.09220577783539	0.0
25	0.0013409798987113182	0.0	0.0	16.495393734047926	0.0
26	0.0013409798987113182	0.0	0.0	16.88561888457292	0.0
27	0.0013409798987113182	0.0	0.0	17.290594813983738	0.0
28	0.0013409798987113182	0.0	0.0	17.71032152228038	0.0
29	0.0013409798987113182	0.0	0.0	18.143458029564137	0.0
30	0.0013409798987113182	0.0	0.0	18.62576379980064	0.0
31	0.0013409798987113182	0.0	0.0	19.069181152974515	0.0
32	0.0013409798987113182	0.0	0.0	19.492930800967294	0.0
33	0.0013409798987113182	0.0	0.0	19.91846842215835	0.0
34	0.0013409798987113182	0.0	0.0	20.33417219075886	0.0
35	0.0013409798987113182	0.0	0.0	20.82184188059021	0.0
36	0.0013409798987113182	0.0	0.0	21.195528279031098	0.0
37	0.0013409798987113182	0.0	0.0	21.60318616823934	0.0
38	0.0013409798987113182	0.0	0.0	22.046156528113645	0.0
39	0.0013409798987113182	0.0	0.0	22.487338914789667	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTAG	20	7.0210965E-4	45.0	1
GAGTATC	25	3.880704E-5	45.0	27
TATGCGG	20	7.0210965E-4	45.0	2
GCGTTGA	20	7.0210965E-4	45.0	17
GAAAGGT	20	7.0210965E-4	45.0	9
GTCCCGA	20	7.0210965E-4	45.0	20
GCGTGAC	20	7.0210965E-4	45.0	35
AGGGCGT	20	7.0210965E-4	45.0	6
TGCGTAG	25	3.880704E-5	45.0	1
ACGGGTA	30	2.1576416E-6	44.999996	5
TAGATGG	60	3.6379788E-12	41.249996	2
ACATACG	55	6.002665E-11	40.909092	17
CGTTTTT	645	0.0	40.813953	1
GCGGGAT	105	0.0	40.714287	5
ATAGCGG	50	1.0732037E-9	40.5	2
TACGAAA	50	1.0732037E-9	40.5	20
CATACGA	50	1.0732037E-9	40.5	18
ATACGAA	50	1.0732037E-9	40.5	19
TGACCCA	45	1.9163053E-8	40.0	11
ATTAGTC	40	3.4416007E-7	39.375	31
>>END_MODULE