FastQCFastQC Report
Sat 18 Jun 2016
SRR3551520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences351338
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31860.9068190745094468No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC9200.26185610437812024TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT6880.19582282588276817TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC4980.14174384780467814TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC4960.1411745954038561No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4410.12552015438125108No Hit
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC3560.10132692734631607TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.0265913E-445.0000041
GCCTTAA207.0265913E-445.00000433
ACGTTAG207.0265913E-445.0000041
TAGCCCG207.0265913E-445.0000041
CTACGAA207.0265913E-445.00000411
TACGAAT207.0265913E-445.00000412
GCTACGA207.0265913E-445.00000410
CACCGGG207.0265913E-445.0000043
AAATCGC253.885258E-545.024
TTAACGG253.885258E-545.02
TTTGCGC253.885258E-545.013
TATCGCG253.885258E-545.01
AATCGCA253.885258E-545.025
AGTACGG603.6379788E-1241.2499962
CGTTTTT13650.040.879121
CCCTCGT800.039.37500414
AGCGCTT403.449095E-739.37500437
AGGGATA1150.039.1304366
ACGGGAT750.039.05
GATAAGT356.2359104E-638.57142632