Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551520_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 351338 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3186 | 0.9068190745094468 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC | 920 | 0.26185610437812024 | TruSeq Adapter, Index 16 (95% over 21bp) |
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT | 688 | 0.19582282588276817 | TruSeq Adapter, Index 13 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC | 498 | 0.14174384780467814 | TruSeq Adapter, Index 16 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCC | 496 | 0.1411745954038561 | No Hit |
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC | 441 | 0.12552015438125108 | No Hit |
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC | 356 | 0.10132692734631607 | TruSeq Adapter, Index 16 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGTACG | 20 | 7.0265913E-4 | 45.000004 | 1 |
GCCTTAA | 20 | 7.0265913E-4 | 45.000004 | 33 |
ACGTTAG | 20 | 7.0265913E-4 | 45.000004 | 1 |
TAGCCCG | 20 | 7.0265913E-4 | 45.000004 | 1 |
CTACGAA | 20 | 7.0265913E-4 | 45.000004 | 11 |
TACGAAT | 20 | 7.0265913E-4 | 45.000004 | 12 |
GCTACGA | 20 | 7.0265913E-4 | 45.000004 | 10 |
CACCGGG | 20 | 7.0265913E-4 | 45.000004 | 3 |
AAATCGC | 25 | 3.885258E-5 | 45.0 | 24 |
TTAACGG | 25 | 3.885258E-5 | 45.0 | 2 |
TTTGCGC | 25 | 3.885258E-5 | 45.0 | 13 |
TATCGCG | 25 | 3.885258E-5 | 45.0 | 1 |
AATCGCA | 25 | 3.885258E-5 | 45.0 | 25 |
AGTACGG | 60 | 3.6379788E-12 | 41.249996 | 2 |
CGTTTTT | 1365 | 0.0 | 40.87912 | 1 |
CCCTCGT | 80 | 0.0 | 39.375004 | 14 |
AGCGCTT | 40 | 3.449095E-7 | 39.375004 | 37 |
AGGGATA | 115 | 0.0 | 39.130436 | 6 |
ACGGGAT | 75 | 0.0 | 39.0 | 5 |
GATAAGT | 35 | 6.2359104E-6 | 38.571426 | 32 |