FastQCFastQC Report
Sat 18 Jun 2016
SRR3551519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403794
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31510.7803483954689767No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC12600.31204029777559844No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC11720.29024700713730267No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC8790.217685255352977No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT8030.19886377707444883No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6170.15280068549805098No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5680.14066578502899993No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC4790.11862484336072354No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC4320.10698524495163377No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4050.10029866714215666No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA302.1619908E-645.00000438
AGCGATG302.1619908E-645.0000041
TATCGCG302.1619908E-645.0000041
TACAAGG351.2093187E-745.0000042
CGACGGT253.8862952E-545.028
ACGCATC253.8862952E-545.024
TAGCGAA207.027844E-445.038
CAACCGG207.027844E-445.02
GCTTACG253.8862952E-545.01
TTGCGAG207.027844E-445.01
ATAAGCG207.027844E-445.012
CGAATAT406.7957444E-945.014
CCGAATA207.027844E-445.021
CGTTTAC207.027844E-445.033
TAGTACG207.027844E-445.01
CGTTTTT14400.042.031251
AAGGGCG1700.041.029415
CGTGTAC501.0786607E-940.518
CGTAAGG501.0786607E-940.52
CTAGAAT451.9230356E-840.041