##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	403794
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.381957136559734	33.0	31.0	34.0	31.0	34.0
2	32.51238255149903	34.0	31.0	34.0	31.0	34.0
3	32.50570340322045	34.0	31.0	34.0	31.0	34.0
4	36.009279484093376	37.0	35.0	37.0	35.0	37.0
5	36.10809472156595	37.0	35.0	37.0	35.0	37.0
6	36.13006632094583	37.0	36.0	37.0	35.0	37.0
7	36.24792839913421	37.0	37.0	37.0	35.0	37.0
8	36.16473251212252	37.0	37.0	37.0	35.0	37.0
9	38.024876545961554	39.0	39.0	39.0	35.0	39.0
10	37.67198868730095	39.0	37.0	39.0	35.0	39.0
11	37.68097594317895	39.0	37.0	39.0	35.0	39.0
12	37.6227754746232	39.0	37.0	39.0	35.0	39.0
13	37.599471512702024	39.0	37.0	39.0	35.0	39.0
14	38.88449060659643	40.0	38.0	41.0	35.0	41.0
15	38.959063284744204	40.0	38.0	41.0	35.0	41.0
16	38.97871934699376	40.0	38.0	41.0	35.0	41.0
17	38.945472196218866	40.0	38.0	41.0	35.0	41.0
18	38.85013397920722	40.0	38.0	41.0	35.0	41.0
19	38.771304179854084	40.0	38.0	41.0	35.0	41.0
20	38.676211137362124	40.0	38.0	41.0	35.0	41.0
21	38.59833479447441	40.0	38.0	41.0	35.0	41.0
22	38.576306235357634	40.0	38.0	41.0	35.0	41.0
23	38.54815821929003	40.0	38.0	41.0	35.0	41.0
24	38.47221107792587	40.0	37.0	41.0	35.0	41.0
25	38.36499799402666	40.0	37.0	41.0	34.0	41.0
26	38.38539205634556	40.0	37.0	41.0	35.0	41.0
27	38.36911147763463	40.0	37.0	41.0	35.0	41.0
28	38.318585714497985	40.0	37.0	41.0	35.0	41.0
29	38.26814415271153	40.0	37.0	41.0	34.0	41.0
30	38.11170795009336	40.0	37.0	41.0	34.0	41.0
31	38.08498888046876	40.0	37.0	41.0	34.0	41.0
32	37.95559369381417	40.0	37.0	41.0	34.0	41.0
33	37.82839764830582	40.0	37.0	41.0	34.0	41.0
34	37.68217457416405	40.0	36.0	41.0	33.0	41.0
35	37.576068490368854	40.0	36.0	41.0	33.0	41.0
36	37.48649806584545	40.0	36.0	41.0	33.0	41.0
37	37.40337647414275	40.0	36.0	41.0	33.0	41.0
38	37.28503147644591	40.0	35.0	41.0	33.0	41.0
39	37.2839319058728	40.0	35.0	41.0	33.0	41.0
40	37.20784607993185	40.0	35.0	41.0	33.0	41.0
41	37.12112116574293	40.0	35.0	41.0	33.0	41.0
42	37.03703373502331	40.0	35.0	41.0	32.0	41.0
43	37.004284362818666	40.0	35.0	41.0	32.0	41.0
44	36.96129462052433	39.0	35.0	41.0	32.0	41.0
45	36.87274204173415	39.0	35.0	41.0	32.0	41.0
46	36.818714493033575	39.0	35.0	41.0	32.0	41.0
47	36.74443156659088	39.0	35.0	41.0	32.0	41.0
48	36.68528011808001	39.0	35.0	41.0	32.0	41.0
49	36.629845911529145	39.0	35.0	41.0	32.0	41.0
50	36.55745008593491	39.0	35.0	41.0	31.0	41.0
51	35.74053849239959	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	12.0
11	11.0
12	7.0
13	12.0
14	11.0
15	15.0
16	27.0
17	45.0
18	88.0
19	121.0
20	206.0
21	387.0
22	551.0
23	844.0
24	1323.0
25	2087.0
26	3050.0
27	3808.0
28	3890.0
29	3994.0
30	4423.0
31	5386.0
32	6713.0
33	9570.0
34	19124.0
35	28831.0
36	25839.0
37	39328.0
38	75492.0
39	168358.0
40	234.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60832999004443	20.82175564768174	24.96396677513782	13.605947587136013
2	34.14860052402958	24.20268750897735	24.31685463379842	17.331857333194648
3	31.923456019653585	22.90004309127922	27.91571940147699	17.260781487590208
4	28.698544307245772	24.523643243832254	27.601202593401585	19.17660985552039
5	24.435479477159145	29.30652758584823	25.884980955635793	20.373011981356832
6	24.822558037018876	34.30437302188739	26.103161513048732	14.769907428044993
7	76.138080308276	6.7252113701540885	11.976651460893425	5.160056860676483
8	76.432289731893	6.524861686899756	11.360743349331589	5.682105231875659
9	69.70113473701937	8.254456480284501	14.125519448035384	7.918889334660744
10	37.359148476698515	22.140249731298635	23.524371337860394	16.976230454142456
11	29.320396043527147	23.777965992560564	25.868140685597112	21.03349727831518
12	25.388688291554608	21.28189126138576	32.02400233782573	21.305418109233916
13	24.35202108005567	22.141487986448535	33.444033343734674	20.062457589761117
14	21.15162681961594	25.602906432487853	31.8900231306062	21.355443617290003
15	20.332892514499967	24.37406202172395	34.029480378608895	21.263565085167187
16	21.847030911801564	23.521151874470647	32.297904376984306	22.33391283674349
17	22.141487986448535	23.866625061293632	30.102725647235967	23.889161305021865
18	22.209096717633248	24.332704299717182	31.20724924094959	22.250949741699976
19	23.615011614833307	24.869116430655236	29.809506827738897	21.70636512677256
20	22.951059203455227	25.648969524064253	30.029916244421663	21.37005502805886
21	23.566472012957103	25.21780908086797	30.220359886476768	20.995359019698164
22	21.38441878779774	24.600415063126245	29.386518868531976	24.62864728054404
23	21.744503385389578	24.552866065369965	30.054433696389744	23.64819685285071
24	22.26432289731893	25.062779536100088	29.684938359658634	22.987959206922344
25	21.936432933624573	26.25720045369669	27.96425900335319	23.842107609325545
26	21.586006726201976	26.09771319038916	28.946442988256386	23.36983709515248
27	23.3235263525461	24.4807996156456	30.188660554639245	22.007013477169053
28	20.197427401100562	25.115529205485963	30.74092235149606	23.94612104191741
29	23.67841027850835	23.908973387420318	29.256749728822122	23.155866605249212
30	22.977805514693138	24.543702977260683	29.810002129798857	22.668489378247326
31	23.111041768822716	25.172241291351533	28.889730902390827	22.826986037434928
32	24.5882801626572	25.501369510195794	27.93503618181548	21.97531414533153
33	23.78118545595031	24.223737846525704	28.14553955729902	23.849537140224967
34	22.485227616061657	24.08530092076653	29.880087371283377	23.549384091888438
35	23.703670683566372	24.133097569552792	28.43999663194599	23.723235114934845
36	23.955531781056678	25.712615838769278	28.83599062888503	21.495861751289024
37	22.95304041169507	25.76511785712517	30.801349202811334	20.480492528368423
38	22.848036374983284	26.59053874005062	27.504618691709137	23.056806193256957
39	24.317597586888365	23.813627740877774	28.26416440065974	23.60461027157412
40	22.851751140432995	23.977820373754934	30.58069213509859	22.589736350713483
41	21.596903371521122	25.462735949518816	28.79810002129799	24.142260657662078
42	22.829462547734735	25.272539958493688	29.72456252445554	22.173434969316038
43	24.286888859170766	23.05730149531692	29.8964323392621	22.759377306250215
44	22.84704577086336	22.87453503519121	29.15001213490047	25.12840705904496
45	22.793305497357565	23.172211573227933	28.769867803880196	25.264615125534306
46	23.76880290445128	24.115514346424167	29.194836971326964	22.92084577779759
47	21.91241078371645	24.33963852855664	31.365993551167183	22.38195713655973
48	21.461933560181677	24.021406955031527	30.45934313040808	24.05731635437872
49	22.736593411492	22.683843742106124	31.340733146109155	23.238829700292722
50	21.880216149818967	21.807654398034643	31.239691525877056	25.072437926269338
51	21.532761754756137	22.456995398643866	29.041540983768954	26.968701862831047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	258.0
1	682.5
2	1107.0
3	3042.5
4	4978.0
5	3275.0
6	1572.0
7	1507.0
8	1442.0
9	1456.5
10	1471.0
11	1481.5
12	1492.0
13	1429.0
14	1366.0
15	1312.5
16	1259.0
17	1238.0
18	1217.0
19	1297.5
20	1378.0
21	1354.0
22	1330.0
23	1496.0
24	1662.0
25	2147.0
26	3110.0
27	3588.0
28	4268.5
29	4949.0
30	5665.5
31	6382.0
32	7305.5
33	8229.0
34	8400.5
35	8572.0
36	9371.5
37	10171.0
38	10903.5
39	11636.0
40	13647.5
41	15659.0
42	17317.5
43	18976.0
44	21306.0
45	23636.0
46	28738.5
47	33841.0
48	37549.0
49	41257.0
50	40762.0
51	40267.0
52	35126.0
53	29985.0
54	26941.5
55	23898.0
56	22004.0
57	20110.0
58	18746.5
59	17383.0
60	16305.0
61	15227.0
62	13883.5
63	12540.0
64	11697.5
65	10855.0
66	9040.0
67	7225.0
68	6333.0
69	5441.0
70	4837.5
71	4234.0
72	3449.5
73	2665.0
74	2289.0
75	1406.0
76	899.0
77	764.0
78	629.0
79	435.5
80	242.0
81	173.5
82	105.0
83	92.5
84	80.0
85	56.0
86	32.0
87	17.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	403794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.891156875937206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9114625572667	26.897882032541474
2	9.401500709459032	6.936644750837761
3	3.535846805751878	3.9132443760082207
4	2.0861333917815097	3.078394968813706
5	1.4994788874441658	2.765875543442925
6	1.2199951764217403	2.700422006475667
7	1.0413730360983464	2.6892219228752583
8	0.9436433800230111	2.7849676773894827
9	0.7620236063261732	2.5300739163731647
>10	6.482805912460791	38.747707348426985
>50	0.06325136322595534	1.6673994708575643
>100	0.047774965840881166	3.190573976318636
>500	0.002691547371317249	0.7116918688326094
>1k	0.002018660528487937	1.3859001408065776
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3151	0.7803483954689767	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	1260	0.31204029777559844	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	1172	0.29024700713730267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	879	0.217685255352977	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	803	0.19886377707444883	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	617	0.15280068549805098	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	568	0.14066578502899993	No Hit
TCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	479	0.11862484336072354	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTC	432	0.10698524495163377	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	405	0.10029866714215666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20431209973402278	0.0
2	0.0	0.0	0.0	1.026265868239746	0.0
3	0.0	0.0	0.0	1.4767430917745188	0.0
4	0.0	0.0	0.0	2.0446069035201115	0.0
5	0.0	0.0	0.0	3.5736043626205443	0.0
6	0.0	0.0	0.0	4.481740689559528	0.0
7	0.0	0.0	0.0	5.356939429511088	0.0
8	0.0	0.0	0.0	6.647944248800131	0.0
9	0.0	0.0	0.0	7.179403359138571	0.0
10	0.0	0.0	0.0	8.328008836188749	0.0
11	0.0	0.0	0.0	10.05587007236363	0.0
12	0.0	0.0	0.0	11.400615165158472	0.0
13	0.0	0.0	0.0	11.881058163320901	0.0
14	0.0	0.0	0.0	12.09131388777446	0.0
15	0.0	0.0	0.0	12.500433389302467	0.0
16	0.0	0.0	0.0	13.30678514291941	0.0
17	0.0	0.0	0.0	14.232009390927058	0.0
18	0.0	0.0	0.0	15.20602089184089	0.0
19	0.0	0.0	0.0	15.810537056023616	0.0
20	0.0	0.0	0.0	16.39821295016766	0.0
21	0.0	0.0	0.0	17.081482141884226	0.0
22	0.0	0.0	0.0	17.783572811879324	0.0
23	0.0	0.0	0.0	18.46981381595566	0.0
24	0.0	0.0	0.0	19.038915882851157	0.0
25	0.0	0.0	0.0	19.570374993189596	0.0
26	0.0	0.0	0.0	20.042397856332684	0.0
27	0.0	0.0	0.0	20.52482206273496	0.0
28	0.0	0.0	0.0	21.014923451066633	0.0
29	0.0	0.0	0.0	21.49833826158883	0.0
30	0.0	0.0	0.0	22.02286314308781	0.0
31	0.0	0.0	0.0	22.512221578329545	0.0
32	0.0	0.0	0.0	23.002570617691198	0.0
33	0.0	0.0	0.0	23.4641921375751	0.0
34	0.0	0.0	0.0	23.931261980118574	0.0
35	0.0	0.0	0.0	24.41343853549087	0.0
36	0.0	0.0	0.0	24.90651173618231	0.0
37	0.0	0.0	0.0	25.388688291554605	0.0
38	0.0	0.0	0.0	25.843375582599048	0.0
39	0.0	0.0	0.0	26.3708722764578	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	30	2.1619908E-6	45.000004	38
AGCGATG	30	2.1619908E-6	45.000004	1
TATCGCG	30	2.1619908E-6	45.000004	1
TACAAGG	35	1.2093187E-7	45.000004	2
CGACGGT	25	3.8862952E-5	45.0	28
ACGCATC	25	3.8862952E-5	45.0	24
TAGCGAA	20	7.027844E-4	45.0	38
CAACCGG	20	7.027844E-4	45.0	2
GCTTACG	25	3.8862952E-5	45.0	1
TTGCGAG	20	7.027844E-4	45.0	1
ATAAGCG	20	7.027844E-4	45.0	12
CGAATAT	40	6.7957444E-9	45.0	14
CCGAATA	20	7.027844E-4	45.0	21
CGTTTAC	20	7.027844E-4	45.0	33
TAGTACG	20	7.027844E-4	45.0	1
CGTTTTT	1440	0.0	42.03125	1
AAGGGCG	170	0.0	41.02941	5
CGTGTAC	50	1.0786607E-9	40.5	18
CGTAAGG	50	1.0786607E-9	40.5	2
CTAGAAT	45	1.9230356E-8	40.0	41
>>END_MODULE