FastQCFastQC Report
Sat 18 Jun 2016
SRR3551517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531923
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81801.5378165636755696No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC10980.20642085414618283RNA PCR Primer, Index 33 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT8730.16412149878835847TruSeq Adapter, Index 1 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC8500.15979756468511422No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC5720.10753436117633566Illumina Paired End PCR Primer 2 (95% over 23bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5620.10565438982709904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG253.8879705E-545.02
ATAGCGG502.1827873E-1145.02
TCGATTG253.8879705E-545.01
TAGCACG253.8879705E-545.01
AAGCCGT207.029866E-445.034
TATAGCG253.8879705E-545.01
CCAACGG253.8879705E-545.02
TGACTAT253.8879705E-545.023
GTATGCG302.163295E-644.9999961
CGTTTTT35600.042.661521
ATGGGCG2050.039.5121965
GCGCGAC2000.039.3759
AATGCGG750.039.0000042
CATACGA1100.038.86363618
CGATTGA356.241962E-638.57142610
TATTGCG356.241962E-638.5714261
TGCGTAG356.241962E-638.5714261
CGCGACC2050.038.41463510
CGGGCTA659.094947E-1238.076926
CGTATGG601.546141E-1037.4999962
Produced by FastQC (version 0.11.5)