##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551516_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	740293
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.404277765695475	33.0	31.0	34.0	31.0	34.0
2	32.537096798159645	34.0	31.0	34.0	31.0	34.0
3	32.52620111226231	34.0	31.0	34.0	31.0	34.0
4	35.99419283986205	37.0	35.0	37.0	35.0	37.0
5	36.113029570724024	37.0	35.0	37.0	35.0	37.0
6	36.13926647962361	37.0	36.0	37.0	35.0	37.0
7	36.26758053905683	37.0	37.0	37.0	35.0	37.0
8	36.19547935749764	37.0	37.0	37.0	35.0	37.0
9	38.051996979574305	39.0	39.0	39.0	35.0	39.0
10	37.683199760094986	39.0	37.0	39.0	35.0	39.0
11	37.6876466480164	39.0	37.0	39.0	35.0	39.0
12	37.643337165149475	39.0	37.0	39.0	35.0	39.0
13	37.64314534920633	39.0	37.0	39.0	35.0	39.0
14	38.98286084023488	40.0	38.0	41.0	35.0	41.0
15	39.04293840411837	40.0	38.0	41.0	35.0	41.0
16	39.06478110694009	40.0	38.0	41.0	35.0	41.0
17	39.01373104973301	40.0	38.0	41.0	35.0	41.0
18	38.87507378835137	40.0	38.0	41.0	35.0	41.0
19	38.72516962878212	40.0	37.0	41.0	35.0	41.0
20	38.58979755313099	40.0	37.0	41.0	35.0	41.0
21	38.49330062556312	40.0	37.0	41.0	35.0	41.0
22	38.48338833407853	40.0	37.0	41.0	35.0	41.0
23	38.42049296697389	40.0	37.0	41.0	35.0	41.0
24	38.343574773772005	40.0	37.0	41.0	35.0	41.0
25	38.231905475264526	40.0	36.0	41.0	34.0	41.0
26	38.236687365678186	40.0	37.0	41.0	34.0	41.0
27	38.23100042820883	40.0	36.0	41.0	35.0	41.0
28	38.158965436658185	40.0	36.0	41.0	34.0	41.0
29	38.07730182508818	40.0	36.0	41.0	34.0	41.0
30	37.890455535848645	40.0	36.0	41.0	34.0	41.0
31	37.808420449740844	40.0	36.0	41.0	34.0	41.0
32	37.60599654461139	40.0	36.0	41.0	33.0	41.0
33	37.37903370692415	40.0	36.0	41.0	33.0	41.0
34	37.14651361015166	40.0	35.0	41.0	32.0	41.0
35	37.02446869009973	40.0	35.0	41.0	32.0	41.0
36	36.91982363739763	40.0	35.0	41.0	31.0	41.0
37	36.859508329809955	40.0	35.0	41.0	31.0	41.0
38	36.705919142825884	40.0	35.0	41.0	31.0	41.0
39	36.68307413416039	40.0	35.0	41.0	31.0	41.0
40	36.607933615473875	40.0	35.0	41.0	31.0	41.0
41	36.504981135847565	39.0	35.0	41.0	31.0	41.0
42	36.34464326962433	39.0	35.0	41.0	30.0	41.0
43	36.322040057112524	39.0	35.0	41.0	30.0	41.0
44	36.27904356788461	39.0	35.0	41.0	30.0	41.0
45	36.17941950011684	39.0	35.0	41.0	30.0	41.0
46	36.113891391651684	39.0	35.0	41.0	30.0	41.0
47	36.06409489215756	39.0	35.0	41.0	30.0	41.0
48	35.985509791393405	39.0	35.0	41.0	29.0	41.0
49	35.92275220757187	39.0	35.0	41.0	29.0	41.0
50	35.816188995438296	39.0	35.0	41.0	29.0	41.0
51	34.89870362140396	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	14.0
10	9.0
11	15.0
12	14.0
13	24.0
14	18.0
15	28.0
16	58.0
17	87.0
18	174.0
19	290.0
20	474.0
21	773.0
22	1167.0
23	1895.0
24	3196.0
25	5558.0
26	8761.0
27	10283.0
28	10082.0
29	9403.0
30	9558.0
31	10646.0
32	13029.0
33	17973.0
34	34264.0
35	50162.0
36	49673.0
37	72281.0
38	136802.0
39	293170.0
40	408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24246345703661	19.278988184408067	23.067893388158474	15.410654970396855
2	37.21769623649014	21.96454647011386	23.610246213323645	17.20751108007235
3	31.668406968592166	22.36155143976777	29.218701243966915	16.751340347673153
4	27.113453727105348	24.445456055913	29.069165857302448	19.37192435967921
5	24.223922149743412	28.753480041010786	27.515186554512876	19.507411254732922
6	24.815849940496534	33.01382020362208	27.419953991189978	14.750375864691412
7	75.81254989578451	6.179985492230779	13.34714768341724	4.660316928567473
8	76.2841199362955	5.602511438038722	13.139392105558203	4.97397652010758
9	69.71753076146877	7.820011806136219	15.693245782413179	6.7692116499818304
10	36.536074230068365	22.30373649352351	24.549333844842515	16.61085543156561
11	29.518717588846577	24.36143526954868	26.918395824356033	19.20145131724871
12	25.647547660183196	21.560652336304678	31.993278337090853	20.79852166642127
13	24.1877202675157	22.218770135608466	33.510650512702405	20.08285908417343
14	21.303186711207587	24.52380341297297	32.647884013491954	21.525125862327485
15	20.79460429856827	24.260799440221643	34.29831161445536	20.64628464675473
16	23.374393652243096	22.724380751945514	33.536180944571946	20.36504465123944
17	23.056546529549788	22.80164745580466	31.390544014329464	22.751262000316093
18	23.221075979375733	23.440988905744074	32.0704099592999	21.26752515558029
19	23.94768017528195	24.519886045119975	30.40985123457874	21.122582545019338
20	24.30564654805597	24.304971139805456	30.692171883294854	20.697210428843714
21	23.83893944694871	24.799640142484126	30.714325273911815	20.647095136655352
22	22.98414276509436	23.813409015079166	30.32650585646494	22.875942363361535
23	22.281718184556656	25.175977619672214	30.43754297284994	22.104761222921194
24	22.67386021480684	23.799225441818304	31.249113526671195	22.277800816703657
25	22.82177462167007	24.58931801327312	29.536278203360023	23.05262916169679
26	22.083283240554756	24.957010264854592	30.732561296675776	22.22714519791488
27	23.24836247269662	23.97158962735025	30.423359399589085	22.356688500364044
28	21.88484829655285	24.95971189785666	30.31029605845253	22.845143747137957
29	22.5017661925751	24.234323436801375	29.88897639178001	23.37493397884351
30	22.93889041230972	24.520831616670698	29.855476142554366	22.684801828465215
31	22.800161557653524	25.39291874973828	28.31162796352255	23.495291729085647
32	23.100583147483498	25.60634775690166	28.36552554191381	22.927543553701035
33	22.331968558395122	25.643089965729786	27.612715505887536	24.41222596998756
34	21.511617697317142	26.38131118354489	28.41834246710424	23.68872865203372
35	22.192834458788617	24.742500604490385	28.2700228152907	24.7946421214303
36	22.1486627592048	27.788321651021956	26.97985797515308	23.083157614620156
37	21.196337125975795	27.635814468055216	27.513160329761327	23.654688076207663
38	21.496488552505564	27.23961998830193	27.593263748272644	23.670627710919863
39	22.125969041987428	26.195303751352505	27.387399313515054	24.291327893145013
40	22.832986398628652	24.533799455080622	29.91464190529966	22.718572240991065
41	21.060309904321667	26.05265752884331	28.92341275684087	23.963619809994153
42	21.96643761321531	26.5919034760561	28.49952653881639	22.9421323719122
43	22.303196166923097	25.528135481491788	29.05889965189459	23.109768699690527
44	22.552691974664086	25.263105283988907	27.91529840211916	24.268904339227845
45	22.17216696632279	24.475038937285643	27.879231601541555	25.47356249485001
46	22.06018427838707	25.493149334115007	28.16074176035705	24.285924627140876
47	21.65034655197334	25.4493828794815	29.359186160074458	23.5410844084707
48	21.032077839450057	24.89190090950475	29.538034264811365	24.53798698623383
49	21.50391804326125	24.419655460743247	29.822516219929135	24.253910276066367
50	20.4822955235292	24.372241801556953	29.967593912140195	25.17786876277366
51	20.628859113891394	24.459774710823957	28.775228186677438	26.13613798860721
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	325.0
1	901.5
2	1478.0
3	8333.5
4	15189.0
5	9809.0
6	4429.0
7	4423.0
8	4417.0
9	4369.0
10	4321.0
11	4237.5
12	4154.0
13	4022.0
14	3890.0
15	3763.0
16	3636.0
17	3503.5
18	3371.0
19	3117.5
20	2864.0
21	3063.0
22	3262.0
23	3376.0
24	3490.0
25	3803.0
26	4776.0
27	5436.0
28	6877.5
29	8319.0
30	8739.0
31	9159.0
32	10327.5
33	11496.0
34	13418.0
35	15340.0
36	16864.0
37	18388.0
38	19968.0
39	21548.0
40	25041.0
41	28534.0
42	31219.0
43	33904.0
44	37774.0
45	41644.0
46	47925.0
47	54206.0
48	60777.5
49	67349.0
50	67041.0
51	66733.0
52	60567.0
53	54401.0
54	49750.0
55	45099.0
56	42118.0
57	39137.0
58	36789.5
59	34442.0
60	33159.0
61	31876.0
62	29007.0
63	26138.0
64	22735.0
65	19332.0
66	16805.5
67	14279.0
68	12448.5
69	10618.0
70	9174.5
71	7731.0
72	6972.5
73	6214.0
74	5245.5
75	3434.0
76	2591.0
77	2107.0
78	1623.0
79	1253.5
80	884.0
81	635.0
82	386.0
83	265.5
84	145.0
85	96.0
86	47.0
87	36.5
88	26.0
89	35.0
90	44.0
91	24.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	740293.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.20480931869657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69648734413613	25.207742970639114
2	8.543021267327827	5.844248269090357
3	3.2913881810018397	3.377439153749385
4	1.992533385346882	2.7261689802770825
5	1.3860391584866976	2.3704602562142076
6	1.140361126978337	2.3403500941648767
7	0.9553000372019586	2.287309893024571
8	0.8136542192036975	2.2264709935372333
9	0.7307413525552642	2.2495381762895317
>10	7.334392588336465	45.16752616671198
>50	0.07950776702217933	1.8475249360355415
>100	0.0329957229285241	2.107105773788869
>500	0.0023852329827848745	0.544045573111078
>1k	7.950776609282914E-4	0.36945059288747784
>5k	3.975388304641457E-4	1.334618170478688
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9815	1.3258263957649201	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	1640	0.2215339061695842	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	1077	0.14548293716136718	Illumina PCR Primer Index 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	884	0.11941217869141003	No Hit
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	789	0.10657942193158655	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1487248967638489	0.0
2	0.0	0.0	0.0	0.6186739574735949	0.0
3	0.0	0.0	0.0	0.8980228098874364	0.0
4	0.0	0.0	0.0	1.282465186081727	0.0
5	0.0	0.0	0.0	2.244381616468074	0.0
6	0.0	0.0	0.0	3.1086340138296595	0.0
7	0.0	0.0	0.0	3.7914717551023718	0.0
8	0.0	0.0	0.0	4.887254100741193	0.0
9	0.0	0.0	0.0	5.409074515090647	0.0
10	0.0	0.0	0.0	6.3150671423341835	0.0
11	0.0	0.0	0.0	7.437190409743169	0.0
12	0.0	0.0	0.0	8.282126131139968	0.0
13	0.0	0.0	0.0	8.652249852423298	0.0
14	0.0	0.0	0.0	8.828261242508034	0.0
15	0.0	0.0	0.0	9.082619989652745	0.0
16	1.35081650103405E-4	0.0	0.0	9.609033180105715	0.0
17	1.35081650103405E-4	0.0	0.0	10.21028160471597	0.0
18	1.35081650103405E-4	0.0	0.0	10.844084707001146	0.0
19	1.35081650103405E-4	0.0	0.0	11.261216842520462	0.0
20	1.35081650103405E-4	0.0	0.0	11.683887325694016	0.0
21	1.35081650103405E-4	0.0	0.0	12.194766126385094	0.0
22	1.35081650103405E-4	0.0	0.0	12.727528154392923	0.0
23	1.35081650103405E-4	0.0	0.0	13.24394530273824	0.0
24	1.35081650103405E-4	0.0	0.0	13.699575708537026	0.0
25	1.35081650103405E-4	0.0	0.0	14.105901312048067	0.0
26	2.7016330020681E-4	0.0	0.0	14.490884014842772	0.0
27	2.7016330020681E-4	0.0	0.0	14.860737572825895	0.0
28	2.7016330020681E-4	0.0	0.0	15.250313051724115	0.0
29	2.7016330020681E-4	1.35081650103405E-4	0.0	15.662177002889397	0.0
30	2.7016330020681E-4	1.35081650103405E-4	0.0	16.11105332618301	0.0
31	2.7016330020681E-4	1.35081650103405E-4	0.0	16.54412509641453	0.0
32	2.7016330020681E-4	1.35081650103405E-4	0.0	16.972198845592217	0.0
33	2.7016330020681E-4	1.35081650103405E-4	0.0	17.391492287513188	0.0
34	4.05244950310215E-4	1.35081650103405E-4	0.0	17.78714644066606	0.0
35	4.05244950310215E-4	1.35081650103405E-4	0.0	18.20792578073817	0.0
36	4.05244950310215E-4	1.35081650103405E-4	0.0	18.606956975143625	0.0
37	5.4032660041362E-4	1.35081650103405E-4	0.0	19.024359273963146	0.0
38	5.4032660041362E-4	1.35081650103405E-4	0.0	19.448650736937942	0.0
39	5.4032660041362E-4	1.35081650103405E-4	0.0	19.86834942380922	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCACA	20	7.0316566E-4	45.000004	39
CGCATCG	40	6.8102963E-9	45.000004	21
ATTCGGA	20	7.0316566E-4	45.000004	17
ATATGCG	20	7.0316566E-4	45.000004	1
GCGTACG	20	7.0316566E-4	45.000004	1
CGTTTTT	4325	0.0	43.283234	1
ACAACGA	150	0.0	40.5	13
CGAATAT	100	0.0	40.5	14
GCGATAC	50	1.0804797E-9	40.5	9
TTAGGCG	40	3.455989E-7	39.375004	1
TCGATAG	40	3.455989E-7	39.375004	1
ACGGGCC	80	0.0	39.375004	5
CGACGTT	35	6.245278E-6	38.571426	27
CTAACGG	35	6.245278E-6	38.571426	2
CGGTCTA	35	6.245278E-6	38.571426	31
TCGACGT	35	6.245278E-6	38.571426	26
CTCGTAA	65	9.094947E-12	38.076927	33
CGAATAG	65	9.094947E-12	38.076927	26
CACAACG	160	0.0	37.968754	12
ACGGGAG	360	0.0	37.5	5
>>END_MODULE