##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551515_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631361
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.338177366039396	33.0	31.0	34.0	30.0	34.0
2	32.45980350385912	34.0	31.0	34.0	31.0	34.0
3	32.46917690513035	34.0	31.0	34.0	31.0	34.0
4	35.94262078272177	37.0	35.0	37.0	35.0	37.0
5	36.033497476087376	37.0	35.0	37.0	35.0	37.0
6	36.06483295610594	37.0	35.0	37.0	35.0	37.0
7	36.1929086528943	37.0	37.0	37.0	35.0	37.0
8	36.08414520377407	37.0	37.0	37.0	35.0	37.0
9	37.94990663027966	39.0	38.0	39.0	35.0	39.0
10	37.672116269455984	39.0	37.0	39.0	35.0	39.0
11	37.6216364330391	39.0	37.0	39.0	35.0	39.0
12	37.52280549479616	39.0	37.0	39.0	35.0	39.0
13	37.47003853579806	39.0	37.0	39.0	35.0	39.0
14	38.7800180879085	40.0	38.0	41.0	35.0	41.0
15	38.816296857107105	40.0	38.0	41.0	35.0	41.0
16	38.87753440583121	40.0	38.0	41.0	35.0	41.0
17	38.7914996333318	40.0	38.0	41.0	35.0	41.0
18	38.7235464338152	40.0	38.0	41.0	35.0	41.0
19	38.657810666164046	40.0	38.0	41.0	35.0	41.0
20	38.52886858706825	40.0	38.0	41.0	35.0	41.0
21	38.460638208568476	40.0	37.0	41.0	35.0	41.0
22	38.43287754549299	40.0	37.0	41.0	35.0	41.0
23	38.38066019282154	40.0	37.0	41.0	34.0	41.0
24	38.29974768793131	40.0	37.0	41.0	34.0	41.0
25	38.21081283132788	40.0	37.0	41.0	34.0	41.0
26	38.21552962568166	40.0	37.0	41.0	34.0	41.0
27	38.1932745291521	40.0	37.0	41.0	34.0	41.0
28	38.11129290532675	40.0	37.0	41.0	34.0	41.0
29	38.07129043447409	40.0	36.0	41.0	34.0	41.0
30	37.93546956495571	40.0	36.0	41.0	34.0	41.0
31	37.904914937729764	40.0	36.0	41.0	34.0	41.0
32	37.762837742591	40.0	36.0	41.0	33.0	41.0
33	37.647865484247525	40.0	36.0	41.0	33.0	41.0
34	37.49625016432754	40.0	36.0	41.0	33.0	41.0
35	37.39828402451212	40.0	36.0	41.0	33.0	41.0
36	37.33030389903716	40.0	35.0	41.0	33.0	41.0
37	37.272267371598815	40.0	35.0	41.0	33.0	41.0
38	37.11217829419302	40.0	35.0	41.0	32.0	41.0
39	37.08060681606878	40.0	35.0	41.0	32.0	41.0
40	37.010836906302416	40.0	35.0	41.0	32.0	41.0
41	36.9008174404184	39.0	35.0	41.0	32.0	41.0
42	36.77061617679901	39.0	35.0	41.0	32.0	41.0
43	36.775150824963845	39.0	35.0	41.0	32.0	41.0
44	36.7677604413323	39.0	35.0	41.0	32.0	41.0
45	36.667833458195865	39.0	35.0	41.0	31.0	41.0
46	36.61114164479592	39.0	35.0	41.0	31.0	41.0
47	36.52214343299634	39.0	35.0	41.0	31.0	41.0
48	36.49154445713308	39.0	35.0	41.0	31.0	41.0
49	36.44255505170576	39.0	35.0	41.0	31.0	41.0
50	36.35688932322395	39.0	35.0	41.0	31.0	41.0
51	35.515472130841154	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	11.0
10	16.0
11	12.0
12	12.0
13	15.0
14	17.0
15	32.0
16	54.0
17	88.0
18	141.0
19	267.0
20	503.0
21	816.0
22	1304.0
23	1912.0
24	2619.0
25	3669.0
26	5077.0
27	6045.0
28	6374.0
29	6621.0
30	7478.0
31	8996.0
32	11531.0
33	16110.0
34	31580.0
35	46321.0
36	42086.0
37	62242.0
38	116636.0
39	252422.0
40	345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18173121241255	19.715661879653638	23.650811500868755	14.451795407065054
2	31.63863463216765	26.520326722746574	24.141022331122766	17.700016313963012
3	31.149057353875197	25.21679356184497	27.120458818330555	16.513690265949275
4	28.074588072433997	24.168106677479287	29.250777289062835	18.506527961023885
5	26.712134579107673	28.064134465068317	25.05159488786922	20.17213606795478
6	26.05054794325275	35.00659685979971	24.32063431222391	14.622220884723635
7	76.68069456301545	6.097620853996367	12.397661559709897	4.824023023278283
8	77.48831492600905	7.756101501359761	9.902417159121327	4.853166413509862
9	71.25257974439346	7.83988874827555	11.912835921129117	8.994695586201871
10	39.135930157231755	27.42567247581019	18.51397219657217	14.92442517038588
11	30.154539162222566	23.481019575171732	26.70801649135756	19.656424771248147
12	26.55944855637266	20.46420352223213	30.687039585910437	22.28930833548477
13	25.133798254881125	22.343635416188203	32.427248436314564	20.09531789261611
14	21.589550193946096	26.275142113624376	29.630591690015695	22.504716002413833
15	19.870565334254096	24.365299725513612	34.15320236758368	21.61093257264861
16	22.769857498325045	22.92887904067562	30.848912112088012	23.45235134891132
17	22.210747892251817	24.288798326155717	29.260122180495785	24.240331601096678
18	22.69335609896715	23.355101122812464	30.666290759169478	23.28525201905091
19	23.61549097901201	24.67019660701247	28.26956368860288	23.444748725372648
20	25.316894771770826	24.836979160892106	28.95665079091043	20.889475276426637
21	24.458907027833522	25.86048235478593	28.4350791385594	21.24553147882115
22	22.881679419539694	23.01472533146647	28.56020565096672	25.543389598027122
23	22.589295189281568	25.20950771428707	29.086212167048647	23.114984929382715
24	23.182299825298045	24.300835813425284	28.942712647756196	23.574151713520475
25	22.62097278735937	26.74697993699326	27.59593956547839	23.036107710168984
26	22.233714150858226	24.983646439992334	28.473884196204708	24.30875521294473
27	23.64400081728203	24.362290353696224	28.30615131438274	23.68755751463901
28	21.341356213006506	24.868023207008353	30.440429484874738	23.350191095110404
29	23.629270734175854	23.820445038575393	28.670443692277477	23.879840534971276
30	23.181191109365322	25.11336620412094	28.354459651451386	23.35098303506235
31	24.025082322158003	25.188283723574944	26.99659940984635	23.790034544420706
32	24.19202326402803	25.686572341338792	27.429632175569918	22.69177221906326
33	22.99825298046601	26.12182253892781	26.206718501776322	24.673205978829863
34	22.180179010106738	25.98259949537586	27.379106406635824	24.458115087881577
35	22.338250224514976	24.830960417257323	27.393994877732393	25.436794480495312
36	23.124329820815667	28.027705227278847	26.337863757818425	22.51010119408706
37	21.28101038866829	27.556025791900357	28.384711757615687	22.778252061815664
38	20.89470208010948	28.06920288076077	27.2452685547571	23.790826484372648
39	22.203778820674703	27.310524406797377	27.88927412367885	22.596422648849074
40	22.253195873676074	25.74691816567701	28.36823940661523	23.631646554031686
41	20.33068244633419	27.236715603276096	26.76440261593605	25.66819933445366
42	20.950137876745632	25.776219943898976	28.09707916706924	25.176563012286156
43	22.568546362540605	24.61872051013604	27.981614322075643	24.831118805247712
44	22.01688099201566	24.06293705186098	29.122166240866953	24.798015715256405
45	21.995973777284313	24.052958608466472	28.201456852735596	25.74961076151362
46	23.31724639310949	25.634145916520023	27.707286322721863	23.341321367648618
47	20.87316131341657	25.626543292981353	30.122069624192815	23.378225769409262
48	21.140995405164396	25.331466466886614	28.619284371381827	24.908253756567163
49	21.009216597160737	24.455580880035352	30.491113641799224	24.044088881004686
50	20.954414352486136	23.693576258273794	30.12032735629854	25.23168203294153
51	20.855263470502614	23.992929560109033	28.02073615570173	27.131070813686627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	274.0
1	752.0
2	1230.0
3	4492.0
4	7754.0
5	4979.0
6	2204.0
7	2136.0
8	2068.0
9	2083.0
10	2098.0
11	2019.5
12	1941.0
13	1934.0
14	1927.0
15	1879.0
16	1831.0
17	1657.0
18	1483.0
19	1498.0
20	1513.0
21	1673.5
22	1834.0
23	2089.0
24	2344.0
25	2790.0
26	3920.0
27	4604.0
28	5778.5
29	6953.0
30	7832.5
31	8712.0
32	10071.5
33	11431.0
34	12607.0
35	13783.0
36	15221.5
37	16660.0
38	18340.5
39	20021.0
40	23036.5
41	26052.0
42	28901.5
43	31751.0
44	44839.5
45	57928.0
46	56799.5
47	55671.0
48	55752.5
49	55834.0
50	49798.5
51	43763.0
52	41008.5
53	38254.0
54	35689.0
55	33124.0
56	32384.5
57	31645.0
58	30597.0
59	29549.0
60	28220.5
61	26892.0
62	25339.0
63	23786.0
64	21433.5
65	19081.0
66	16747.0
67	14413.0
68	12160.0
69	9907.0
70	8366.5
71	6826.0
72	5983.5
73	5141.0
74	4188.5
75	2694.0
76	2152.0
77	1708.0
78	1264.0
79	962.0
80	660.0
81	511.0
82	362.0
83	244.0
84	126.0
85	76.5
86	27.0
87	19.0
88	11.0
89	7.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	631361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.530684977126377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36614439557017	22.093879575503923
2	8.313651961238602	5.076429780760893
3	3.1594368525709586	2.8937931375290282
4	1.9043953746724118	2.3256998102407986
5	1.3252108030070915	2.0229796777447095
6	1.125448143804032	2.0616421641943514
7	0.9845813924322525	2.104196102868268
8	0.9018943358621957	2.2028361480690646
9	0.8528585700580494	2.3434520699236296
>10	8.930871570311302	48.55122755846708
>50	0.08026160181865429	1.6456147963445726
>100	0.046384951700349404	2.5831474186655514
>500	0.006254150791058346	1.323556746855985
>1k	0.002605896162940978	2.7715450128321164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4833	0.7654891575501179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	3748	0.5936381879780348	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	3488	0.5524573104768904	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	3369	0.5336091396205974	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1980	0.31360822097025315	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	900	0.14254919135011507	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	847	0.1341546278594972	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	844	0.13367946388833013	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	843	0.1335210758979411	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	798	0.12639361633043536	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	653	0.10342735772402793	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5838799038901674E-4	0.0	0.0	0.1457169511578954	0.0
2	1.5838799038901674E-4	0.0	0.0	0.7667562614732301	0.0
3	1.5838799038901674E-4	0.0	0.0	1.0821067503377624	0.0
4	1.5838799038901674E-4	0.0	0.0	1.5249595714654531	0.0
5	1.5838799038901674E-4	0.0	0.0	2.951243424918549	0.0
6	1.5838799038901674E-4	0.0	0.0	3.698359575583541	0.0
7	1.5838799038901674E-4	0.0	0.0	4.451336081892927	0.0
8	1.5838799038901674E-4	0.0	0.0	5.49131162678721	0.0
9	1.5838799038901674E-4	0.0	0.0	5.939549639588128	0.0
10	1.5838799038901674E-4	0.0	0.0	7.31166480032818	0.0
11	1.5838799038901674E-4	0.0	0.0	8.325189550827497	0.0
12	1.5838799038901674E-4	0.0	0.0	9.671170693153362	0.0
13	1.5838799038901674E-4	0.0	0.0	10.015822960239863	0.0
14	1.5838799038901674E-4	0.0	0.0	10.154412451830252	0.0
15	1.5838799038901674E-4	0.0	0.0	10.499698270878309	0.0
16	1.5838799038901674E-4	0.0	0.0	10.987374893286091	0.0
17	1.5838799038901674E-4	0.0	0.0	11.561372970455889	0.0
18	1.5838799038901674E-4	0.0	0.0	12.228819961955205	0.0
19	1.5838799038901674E-4	0.0	0.0	12.71760530029571	0.0
20	1.5838799038901674E-4	0.0	0.0	13.119118855931868	0.0
21	1.5838799038901674E-4	0.0	0.0	13.58097823590624	0.0
22	1.5838799038901674E-4	0.0	0.0	14.08180106151631	0.0
23	1.5838799038901674E-4	0.0	0.0	14.566309924116315	0.0
24	1.5838799038901674E-4	0.0	0.0	14.982078398887483	0.0
25	1.5838799038901674E-4	0.0	0.0	15.345578836830276	0.0
26	1.5838799038901674E-4	0.0	0.0	15.678035228656823	0.0
27	1.5838799038901674E-4	0.0	0.0	16.034249819041722	0.0
28	1.5838799038901674E-4	0.0	0.0	16.403610612628906	0.0
29	1.5838799038901674E-4	0.0	0.0	16.760617142965753	0.0
30	1.5838799038901674E-4	0.0	0.0	17.18778955304493	0.0
31	1.5838799038901674E-4	0.0	0.0	17.553982586824336	0.0
32	1.5838799038901674E-4	0.0	0.0	17.909563625247678	0.0
33	1.5838799038901674E-4	0.0	0.0	18.271955347257748	0.0
34	1.5838799038901674E-4	0.0	0.0	18.634663845248596	0.0
35	1.5838799038901674E-4	0.0	0.0	19.01954666189391	0.0
36	1.5838799038901674E-4	0.0	0.0	19.38684841160604	0.0
37	1.5838799038901674E-4	0.0	0.0	19.756367593183615	0.0
38	1.5838799038901674E-4	0.0	0.0	20.127153878684304	0.0
39	1.5838799038901674E-4	0.0	0.0	20.493980464425267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACG	20	7.030869E-4	45.0	17
TCCGATA	20	7.030869E-4	45.0	14
TAAGTCG	20	7.030869E-4	45.0	10
GTCCGTT	20	7.030869E-4	45.0	9
CGTAAGG	75	0.0	45.0	2
CGTTTAG	20	7.030869E-4	45.0	27
GGTTACG	20	7.030869E-4	45.0	1
CGTTTTT	2115	0.0	41.489365	1
CGGTCTA	40	3.4549157E-7	39.375	31
ATAGTAG	40	3.4549157E-7	39.375	1
CGAATAT	75	0.0	39.0	14
TACGGCT	435	0.0	38.793102	7
TACGCGG	70	0.0	38.57143	2
TTACGGG	105	0.0	38.57143	3
ATAACGC	35	6.2438157E-6	38.57143	11
ATAGTCG	35	6.2438157E-6	38.57143	1
TATTAGT	130	0.0	38.076927	30
CTACGAA	90	0.0	37.5	11
CTAACGG	60	1.546141E-10	37.499996	2
ACATGCG	30	1.1393393E-4	37.499996	1
>>END_MODULE