##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591338
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.279156759755	33.0	31.0	34.0	30.0	34.0
2	32.40057801122201	34.0	31.0	34.0	30.0	34.0
3	32.40223526984567	34.0	31.0	34.0	30.0	34.0
4	35.908194636569945	37.0	35.0	37.0	35.0	37.0
5	36.00272433024767	37.0	35.0	37.0	35.0	37.0
6	36.01863063087439	37.0	35.0	37.0	35.0	37.0
7	36.14913805640767	37.0	36.0	37.0	35.0	37.0
8	36.00726995390115	37.0	36.0	37.0	35.0	37.0
9	37.87338375007187	39.0	38.0	39.0	35.0	39.0
10	37.549795548400404	39.0	37.0	39.0	35.0	39.0
11	37.53098058978114	39.0	37.0	39.0	35.0	39.0
12	37.45971846896361	39.0	37.0	39.0	35.0	39.0
13	37.43832123083583	39.0	37.0	39.0	35.0	39.0
14	38.749376836935895	40.0	38.0	41.0	35.0	41.0
15	38.76871095718523	40.0	38.0	41.0	35.0	41.0
16	38.835878972770224	40.0	38.0	41.0	35.0	41.0
17	38.73906462970416	40.0	38.0	41.0	35.0	41.0
18	38.649038282674205	40.0	38.0	41.0	35.0	41.0
19	38.536456307560144	40.0	37.0	41.0	35.0	41.0
20	38.36229871917583	40.0	37.0	41.0	34.0	41.0
21	38.316152521907945	40.0	37.0	41.0	34.0	41.0
22	38.28372436745144	40.0	37.0	41.0	34.0	41.0
23	38.19214899093243	40.0	37.0	41.0	34.0	41.0
24	38.1330964693627	40.0	36.0	41.0	34.0	41.0
25	38.057236301404615	40.0	36.0	41.0	34.0	41.0
26	38.050801741136205	40.0	36.0	41.0	34.0	41.0
27	38.00699599890418	40.0	36.0	41.0	34.0	41.0
28	37.920214834832194	40.0	36.0	41.0	34.0	41.0
29	37.860545068979164	40.0	36.0	41.0	34.0	41.0
30	37.693928007332524	40.0	36.0	41.0	33.0	41.0
31	37.6477141668555	40.0	36.0	41.0	33.0	41.0
32	37.415305628929644	40.0	35.0	41.0	33.0	41.0
33	37.23093560704707	40.0	35.0	41.0	33.0	41.0
34	37.06562236825639	40.0	35.0	41.0	32.0	41.0
35	36.88685658625017	40.0	35.0	41.0	31.0	41.0
36	36.81145977427461	40.0	35.0	41.0	31.0	41.0
37	36.70735349326443	40.0	35.0	41.0	31.0	41.0
38	36.60063787546209	40.0	35.0	41.0	30.0	41.0
39	36.573878221930606	40.0	35.0	41.0	31.0	41.0
40	36.50529308111435	39.0	35.0	41.0	30.0	41.0
41	36.413636532744384	39.0	35.0	41.0	30.0	41.0
42	36.26318619807961	39.0	35.0	41.0	30.0	41.0
43	36.25091233778313	39.0	35.0	41.0	30.0	41.0
44	36.23839327085356	39.0	35.0	41.0	30.0	41.0
45	36.15641646570997	39.0	35.0	41.0	30.0	41.0
46	36.0705231187578	39.0	35.0	41.0	29.0	41.0
47	35.96205046859833	39.0	35.0	41.0	29.0	41.0
48	35.911449966009286	39.0	35.0	41.0	29.0	41.0
49	35.880208949873	39.0	35.0	41.0	29.0	41.0
50	35.77840930229412	38.0	35.0	41.0	29.0	41.0
51	34.90247202107762	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	4.0
10	11.0
11	10.0
12	13.0
13	16.0
14	21.0
15	27.0
16	53.0
17	104.0
18	174.0
19	279.0
20	580.0
21	1104.0
22	1775.0
23	2481.0
24	3435.0
25	4914.0
26	6346.0
27	7453.0
28	7271.0
29	7163.0
30	7677.0
31	9204.0
32	11703.0
33	15861.0
34	29751.0
35	42872.0
36	40642.0
37	58818.0
38	107239.0
39	224028.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2375392753383	18.74258038549865	22.644917120158013	15.374963219005036
2	32.1589344841698	27.58016565821916	23.130088037636682	17.13081181997436
3	30.11864618881249	26.341618499064833	27.74301668419753	15.796718627925147
4	26.797702836617976	23.62844938089553	31.654823468134975	17.91902431435152
5	27.160777761618565	27.302321176721268	26.226625043545315	19.310276018114852
6	24.06897578034897	35.4709151111547	26.109433183729102	14.350675924767224
7	73.78960932664567	6.323456297413661	15.33200978120804	4.554924594732624
8	73.71773841694598	9.029861094670053	11.980457876882596	5.271942611501375
9	67.85036645708546	7.725869130683298	13.901355908126995	10.52240850410425
10	39.03283739587173	22.14198986028295	22.418819693643908	16.406353050201407
11	30.808099597861123	23.47726680849193	25.95114807436694	19.763485519280007
12	24.868180296209612	21.273620163087777	31.509221460484525	22.34897808021808
13	24.291860154429447	22.56662010559105	33.94995755388627	19.191562186093233
14	21.11905543022772	27.12272845648343	30.616161991957224	21.142054121331626
15	20.006493748076394	23.325238695974214	35.27086032015531	21.39740723579408
16	22.099543746554424	23.169151990908755	31.231038762940994	23.500265499595834
17	22.241763593748416	24.839939256398203	30.272703597604078	22.645593552249306
18	22.319891500292556	23.731605274817447	30.83667885371818	23.111824371171817
19	23.012557961774824	25.779165215156137	28.657721979646155	22.550554843422884
20	24.51761936489791	25.004650470627627	30.412217716432906	20.06551244804156
21	23.220391721824065	27.621089799742276	29.17214858507317	19.986369893360482
22	22.01837189559947	23.88718465581444	29.687758946659947	24.40668450192614
23	22.748580338148404	26.069523690342916	29.243681278727223	21.938214692781454
24	22.722368594610863	25.093601290632428	29.127334959025124	23.05669515573158
25	21.53945797496525	28.3311743875753	27.676049907159694	22.45331773029976
26	21.385738782219306	25.347094216843836	29.656304854414905	23.610862146521956
27	23.831548116305733	25.726234404012594	28.37040744887019	22.07181003081148
28	21.57683084800909	26.15171018943481	30.4396132161302	21.831845746425902
29	24.110069029894916	24.16012500465047	28.38478163081013	23.345024334644485
30	22.724228782861918	27.32126127527742	28.268266203085208	21.686243738775453
31	25.638467340167555	24.84230676871772	25.214344418927926	24.304881472186803
32	24.96643205746967	26.40892349214831	25.817383628314094	22.80726082206792
33	23.22918533901085	25.383114225705093	26.355147140890654	25.032553294393395
34	23.555056498990425	24.692646168519527	26.42059194572309	25.331705386766963
35	23.150550108398242	24.984019291843243	26.1835024977255	25.68192810203302
36	24.51288434025887	27.916183299568097	25.236328461894892	22.33460389827814
37	22.110366660015085	27.690254981076812	26.6000155579381	23.599362800970002
38	22.3417064352367	26.15576878198255	26.579722595199364	24.922802187581382
39	22.69091450236582	25.221616063909302	27.030057259976527	25.057412173748343
40	21.920120134339413	23.870781177600627	29.72462449563532	24.48447419242464
41	20.346062657904614	26.747985077908066	26.5303430525351	26.37560921165222
42	21.54791337610639	25.695625851881665	28.24256178361614	24.51389898839581
43	23.100155917597043	25.43908898125945	27.44065153939033	24.020103561753174
44	22.57981053137123	24.26277357450392	28.145155562470194	25.012260331654655
45	22.868647034352605	23.544233585529764	27.37334654630685	26.213772833810783
46	24.305050580209624	25.66890678427566	26.7045243160426	23.321518319472112
47	21.02672244976646	24.967784921652253	30.343898075212483	23.6615945533688
48	21.49633542914543	25.118629278010207	28.07193178858791	25.31310350425645
49	22.0860151047286	23.741582648163995	30.26035871193801	23.912043535169396
50	21.7428949264211	23.582451998687723	29.418369866303195	25.25628320858798
51	21.565838826525606	23.303085544984427	27.358295932275617	27.77277969621435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307.0
1	759.0
2	1211.0
3	5156.5
4	9102.0
5	6012.0
6	2922.0
7	2832.0
8	2742.0
9	2724.0
10	2706.0
11	2665.5
12	2625.0
13	2581.0
14	2537.0
15	2534.5
16	2532.0
17	2376.0
18	2220.0
19	2239.0
20	2258.0
21	2163.5
22	2069.0
23	2197.5
24	2326.0
25	2853.5
26	3998.0
27	4615.0
28	5658.0
29	6701.0
30	7479.5
31	8258.0
32	9502.5
33	10747.0
34	12353.5
35	13960.0
36	14534.0
37	15108.0
38	15887.5
39	16667.0
40	18954.0
41	21241.0
42	23476.5
43	25712.0
44	28283.0
45	30854.0
46	33547.5
47	36241.0
48	50217.0
49	64193.0
50	58509.5
51	52826.0
52	51080.0
53	49334.0
54	44179.0
55	39024.0
56	34977.5
57	30931.0
58	28651.0
59	26371.0
60	25219.0
61	24067.0
62	21886.0
63	19705.0
64	17933.5
65	16162.0
66	13788.5
67	11415.0
68	10071.0
69	8727.0
70	7677.5
71	6628.0
72	5740.5
73	4853.0
74	4072.5
75	2701.5
76	2111.0
77	1755.5
78	1400.0
79	1028.0
80	656.0
81	504.5
82	353.0
83	239.0
84	125.0
85	107.0
86	89.0
87	58.0
88	27.0
89	15.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.912844563426205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.07230186165754	24.78089614926151
2	8.555039269930658	5.802514339903312
3	3.4500415126383612	3.510021646664177
4	2.107187409961347	2.8584287640011117
5	1.5621574822396396	2.6488601939393064
6	1.2088065340984742	2.4596440858881303
7	1.0398195308704452	2.468426668709691
8	0.906006044997941	2.4580193742031757
9	0.8117211836743238	2.477499689770891
>10	7.176377668057161	41.103698881009336
>50	0.06902591519751186	1.611804383564847
>100	0.033512582016089754	2.350873179517762
>500	0.004501690121564295	1.2123306597888335
>1k	0.002500938956424608	2.381915086715377
>5k	0.0010003755825698432	1.875066897062511
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5827	0.9853924489885649	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTC	5227	0.8839276352948736	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGC	4974	0.8411433055207005	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCG	4879	0.8250780433525328	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	2014	0.340583557965157	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCT	1165	0.19701084658858387	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGCT	1010	0.17079910305104695	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCT	997	0.1686006987543503	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTCCAT	917	0.1550720569285248	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	907	0.15338097670029663	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTCCATCGTA	844	0.14272717126245904	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC	826	0.1396832268516483	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTC	788	0.1332571219843812	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCC	747	0.1263236930486456	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6910802282281876E-4	0.0	0.0	0.1750268036216174	0.0
2	1.6910802282281876E-4	0.0	0.0	0.7921019789020831	0.0
3	1.6910802282281876E-4	0.0	0.0	1.107657549489463	0.0
4	1.6910802282281876E-4	0.0	0.0	1.6452519540432038	0.0
5	1.6910802282281876E-4	0.0	0.0	3.384020644707426	0.0
6	1.6910802282281876E-4	0.0	0.0	4.163946845966266	0.0
7	1.6910802282281876E-4	0.0	0.0	4.962982253804085	0.0
8	1.6910802282281876E-4	0.0	0.0	6.175317669420872	0.0
9	3.382160456456375E-4	0.0	0.0	6.681119765683923	0.0
10	3.382160456456375E-4	0.0	0.0	8.36847961741001	0.0
11	3.382160456456375E-4	0.0	0.0	9.508775015304277	0.0
12	3.382160456456375E-4	0.0	0.0	11.272233477300631	0.0
13	3.382160456456375E-4	0.0	0.0	11.704304475612933	0.0
14	3.382160456456375E-4	0.0	0.0	11.906050346840555	0.0
15	3.382160456456375E-4	0.0	0.0	12.309880305341446	0.0
16	3.382160456456375E-4	0.0	0.0	12.851025978374466	0.0
17	3.382160456456375E-4	0.0	0.0	13.446286218710789	0.0
18	5.073240684684562E-4	0.0	0.0	14.109696992244706	0.0
19	5.073240684684562E-4	0.0	0.0	14.69007572657262	0.0
20	5.073240684684562E-4	0.0	0.0	15.098978925758196	0.0
21	5.073240684684562E-4	0.0	0.0	15.597509377039865	0.0
22	5.073240684684562E-4	0.0	0.0	16.109399362124538	0.0
23	5.073240684684562E-4	0.0	0.0	16.603533004812814	0.0
24	5.073240684684562E-4	0.0	0.0	17.012267095975567	0.0
25	5.073240684684562E-4	0.0	0.0	17.37770953329568	0.0
26	6.76432091291275E-4	0.0	0.0	17.747210563163538	0.0
27	6.76432091291275E-4	0.0	0.0	18.125505210218183	0.0
28	8.455401141140938E-4	0.0	0.0	18.504138073318476	0.0
29	8.455401141140938E-4	0.0	0.0	18.881925396304652	0.0
30	8.455401141140938E-4	0.0	0.0	19.32160625564398	0.0
31	8.455401141140938E-4	0.0	0.0	19.68873977319232	0.0
32	8.455401141140938E-4	0.0	0.0	20.083776114506424	0.0
33	8.455401141140938E-4	0.0	0.0	20.45953414121873	0.0
34	8.455401141140938E-4	0.0	0.0	20.83106446736046	0.0
35	8.455401141140938E-4	0.0	0.0	21.209359114415108	0.0
36	8.455401141140938E-4	0.0	0.0	21.564316854320204	0.0
37	0.0010146481369369125	0.0	0.0	21.94683920194542	0.0
38	0.0010146481369369125	0.0	0.0	22.314818259607872	0.0
39	0.0010146481369369125	0.0	0.0	22.689730746206063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	25	3.8885006E-5	45.0	21
TAGCGAG	25	3.8885006E-5	45.0	1
TAACGCC	20	7.030506E-4	45.0	12
TCTAGCG	20	7.030506E-4	45.0	1
ATTCCGG	20	7.030506E-4	45.0	2
CGTAAGG	25	3.8885006E-5	45.0	2
TGTAGCG	35	1.210592E-7	45.0	1
GATTACG	20	7.030506E-4	45.0	1
ATCTCGG	30	2.163708E-6	44.999996	2
CGTTTTT	2810	0.0	42.51779	1
TACGGCT	630	0.0	42.5	7
GCTACGA	85	0.0	42.35294	10
CGTTAGG	70	0.0	41.785713	2
TTGAGCG	50	1.0804797E-9	40.5	1
GTATGCG	45	1.9255822E-8	40.000004	1
CGAATAT	90	0.0	40.000004	14
ACGGCTG	675	0.0	39.666668	8
CGATGAA	580	0.0	39.56897	19
TCACGAC	40	3.4544246E-7	39.375	25
TCGAATG	80	0.0	39.375	44
>>END_MODULE