Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551509_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 647162 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 7774 | 1.2012448196896603 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC | 2511 | 0.38800176771812933 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG | 2444 | 0.3776488730796926 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC | 2315 | 0.3577156878803144 | No Hit |
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC | 1590 | 0.24568809664349883 | RNA PCR Primer, Index 35 (95% over 24bp) |
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC | 1066 | 0.16471918932199356 | No Hit |
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC | 1053 | 0.16271041872050584 | RNA PCR Primer, Index 10 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT | 789 | 0.1219169234287551 | TruSeq Adapter, Index 10 (95% over 24bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 788 | 0.12176240261325603 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC | 655 | 0.1012111341518816 | No Hit |
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG | 649 | 0.10028400925888727 | TruSeq Adapter, Index 10 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATGCG | 25 | 3.888909E-5 | 45.000004 | 1 |
CACAACG | 50 | 2.1827873E-11 | 45.000004 | 12 |
GCGTAAG | 40 | 6.8066583E-9 | 45.0 | 1 |
ATCGCAT | 30 | 2.1640262E-6 | 44.999996 | 10 |
AATATAC | 170 | 0.0 | 42.35294 | 16 |
CGAATAT | 170 | 0.0 | 42.35294 | 14 |
CGTAAGG | 85 | 0.0 | 42.35294 | 2 |
ACGGGTA | 80 | 0.0 | 42.1875 | 5 |
CGTTTTT | 3240 | 0.0 | 42.083332 | 1 |
TACGAAT | 175 | 0.0 | 41.142857 | 12 |
ACTACGG | 45 | 1.9261279E-8 | 40.0 | 2 |
CCAATCG | 35 | 6.2440595E-6 | 38.571426 | 24 |
AATCGGT | 35 | 6.2440595E-6 | 38.571426 | 26 |
TAGTAAG | 35 | 6.2440595E-6 | 38.571426 | 1 |
GCTACGA | 190 | 0.0 | 37.894737 | 10 |
AGCTACG | 190 | 0.0 | 37.894737 | 9 |
GACCTAT | 155 | 0.0 | 37.741936 | 33 |
ATACGAA | 90 | 0.0 | 37.5 | 19 |
ATTAGCG | 30 | 1.139371E-4 | 37.499996 | 1 |
CTAAGCG | 30 | 1.139371E-4 | 37.499996 | 1 |