FastQCFastQC Report
Sat 18 Jun 2016
SRR3551509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647162
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77741.2012448196896603No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC25110.38800176771812933No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG24440.3776488730796926No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC23150.3577156878803144No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC15900.24568809664349883RNA PCR Primer, Index 35 (95% over 24bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10660.16471918932199356No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC10530.16271041872050584RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT7890.1219169234287551TruSeq Adapter, Index 10 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT7880.12176240261325603No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC6550.1012111341518816No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG6490.10028400925888727TruSeq Adapter, Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCG253.888909E-545.0000041
CACAACG502.1827873E-1145.00000412
GCGTAAG406.8066583E-945.01
ATCGCAT302.1640262E-644.99999610
AATATAC1700.042.3529416
CGAATAT1700.042.3529414
CGTAAGG850.042.352942
ACGGGTA800.042.18755
CGTTTTT32400.042.0833321
TACGAAT1750.041.14285712
ACTACGG451.9261279E-840.02
CCAATCG356.2440595E-638.57142624
AATCGGT356.2440595E-638.57142626
TAGTAAG356.2440595E-638.5714261
GCTACGA1900.037.89473710
AGCTACG1900.037.8947379
GACCTAT1550.037.74193633
ATACGAA900.037.519
ATTAGCG301.139371E-437.4999961
CTAAGCG301.139371E-437.4999961