##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	647162
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35849756320674	33.0	31.0	34.0	31.0	34.0
2	32.49189847364338	34.0	31.0	34.0	31.0	34.0
3	32.48104493156273	34.0	31.0	34.0	31.0	34.0
4	36.0092109858119	37.0	35.0	37.0	35.0	37.0
5	36.08605418735958	37.0	35.0	37.0	35.0	37.0
6	36.09364270460874	37.0	36.0	37.0	35.0	37.0
7	36.2006530049663	37.0	37.0	37.0	35.0	37.0
8	36.10044934653147	37.0	37.0	37.0	35.0	37.0
9	37.94397693313266	39.0	38.0	39.0	35.0	39.0
10	37.721062114277416	39.0	37.0	39.0	35.0	39.0
11	37.64687976117263	39.0	37.0	39.0	35.0	39.0
12	37.55449022037759	39.0	37.0	39.0	35.0	39.0
13	37.51979102604912	39.0	37.0	39.0	35.0	39.0
14	38.85514909713488	40.0	38.0	41.0	35.0	41.0
15	38.8813218328641	40.0	38.0	41.0	35.0	41.0
16	38.921279370544006	40.0	38.0	41.0	35.0	41.0
17	38.846349136692204	40.0	38.0	41.0	35.0	41.0
18	38.73831436332788	40.0	38.0	41.0	35.0	41.0
19	38.6257397684042	40.0	37.0	41.0	35.0	41.0
20	38.45884492599998	40.0	37.0	41.0	35.0	41.0
21	38.37271811385712	40.0	37.0	41.0	35.0	41.0
22	38.34749568114321	40.0	37.0	41.0	35.0	41.0
23	38.2948257159722	40.0	37.0	41.0	34.0	41.0
24	38.20910374836594	40.0	36.0	41.0	34.0	41.0
25	38.09734193293178	40.0	36.0	41.0	34.0	41.0
26	38.096815016950934	40.0	36.0	41.0	34.0	41.0
27	38.071255728859235	40.0	36.0	41.0	34.0	41.0
28	37.96424697370983	40.0	36.0	41.0	34.0	41.0
29	37.89264357301572	40.0	36.0	41.0	34.0	41.0
30	37.744624993432865	40.0	36.0	41.0	33.0	41.0
31	37.65462434444544	40.0	35.0	41.0	33.0	41.0
32	37.46136979612523	40.0	35.0	41.0	33.0	41.0
33	37.31228038729097	40.0	35.0	41.0	33.0	41.0
34	37.10555780469187	40.0	35.0	41.0	32.0	41.0
35	36.991187677892086	40.0	35.0	41.0	32.0	41.0
36	36.83028360750477	40.0	35.0	41.0	31.0	41.0
37	36.73035963174599	40.0	35.0	41.0	31.0	41.0
38	36.55034751731406	39.0	35.0	41.0	31.0	41.0
39	36.53132600492612	39.0	35.0	41.0	31.0	41.0
40	36.46623411139715	39.0	35.0	41.0	31.0	41.0
41	36.3646923027001	39.0	35.0	41.0	30.0	41.0
42	36.254047672762	39.0	35.0	41.0	30.0	41.0
43	36.21919704803434	39.0	35.0	41.0	30.0	41.0
44	36.1516559995797	39.0	35.0	41.0	30.0	41.0
45	36.042340248654895	39.0	35.0	41.0	30.0	41.0
46	35.96338783797565	38.0	35.0	41.0	30.0	41.0
47	35.8635627555388	38.0	35.0	41.0	30.0	41.0
48	35.78392272723058	38.0	35.0	41.0	30.0	41.0
49	35.72085042076018	38.0	35.0	40.0	29.0	41.0
50	35.619319428520214	37.0	35.0	40.0	29.0	41.0
51	34.73990438251937	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	13.0
10	13.0
11	10.0
12	14.0
13	22.0
14	35.0
15	42.0
16	75.0
17	98.0
18	163.0
19	322.0
20	571.0
21	958.0
22	1470.0
23	2107.0
24	2986.0
25	4548.0
26	6549.0
27	8070.0
28	8107.0
29	7927.0
30	8391.0
31	9730.0
32	12281.0
33	17063.0
34	33847.0
35	48504.0
36	46722.0
37	69595.0
38	124325.0
39	232287.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.614832144038125	18.33590352956447	22.70374342127628	16.345520905121127
2	34.21291732209246	25.269561562638103	23.23884900534951	17.278672109919928
3	31.26651441215646	24.247715409742845	28.263865925378806	16.221904252721885
4	28.122942941643668	24.186061604358724	29.169975987465275	18.521019466532337
5	26.425531783386543	27.814673914723052	26.048964556015342	19.710829745875067
6	25.696039013415493	34.0098769705267	25.301701892261907	14.992382123795897
7	74.72209431332494	6.739579888806821	13.576662412193546	4.961663385674684
8	75.61816052240398	7.54664210815839	11.770314079009584	5.064883290428054
9	69.04206365639516	8.131657915637817	14.092452894329396	8.733825533637637
10	40.62800349835126	23.30931049721708	20.023888918076153	16.0387970863555
11	30.718738121212308	22.92764408293441	25.912831717560675	20.440786078292604
12	25.891662365837302	20.917173752476195	31.448694453629848	21.742469428056655
13	24.836748758425248	21.741851344794657	33.21084983358108	20.210550063199015
14	21.87891130814232	24.71050525216252	31.302054199721248	22.10852923997392
15	21.0146763870561	22.717650294671195	34.121595520132516	22.146077798140187
16	23.115077832134766	22.430241577842953	31.449776099338344	23.004904490683938
17	23.230659402128058	23.160815993522487	29.714198299652946	23.894326304696506
18	23.769628006588768	22.46516328214574	30.413250468970677	23.35195824229482
19	24.645915551283913	23.460740896406154	28.691579542680195	23.201764009629738
20	25.53765517752897	23.433081670431825	29.390786232813422	21.638476919225788
21	25.36969105108149	24.89963873033336	28.606747614971212	21.12392260361393
22	23.736251510440972	22.69709902620982	28.85212666998371	24.714522793365497
23	23.820928917334456	24.086859240808327	28.657430442454903	23.43478139940231
24	23.70009363961419	23.636122021997583	29.046050293435027	23.617734044953195
25	23.63674010525958	25.02989977779907	28.266956341688786	23.066403775252564
26	23.07397529521202	24.341045982304276	28.608756385572697	23.976222336911004
27	25.13667366130892	23.22262431972211	28.601648428059747	23.03905359090923
28	23.164060930647967	24.082996220420853	30.244668259261204	22.508274589669973
29	24.658895299785836	23.42906412922885	28.563018224184976	23.34902234680034
30	24.909682583340803	23.79064283749664	28.562091099291987	22.737583479870572
31	26.056690596790293	23.736715072887467	26.93591403697992	23.270680293342316
32	25.50427868138117	24.309987298388965	28.06561571909352	22.120118301136348
33	24.743572706679316	23.234985984962034	27.621213853718235	24.400227454640415
34	24.83304025885327	23.501534391697906	28.552510808731046	23.11291454071778
35	25.70391957500595	22.576881831751557	28.735617975097426	22.98358061814507
36	25.724625364282822	24.752071351531765	27.33828624053946	22.18501704364595
37	24.78838374317404	24.991424094739802	28.58758703384933	21.632605128236825
38	22.740673896180557	24.959283765115998	29.480408305802875	22.81963403290057
39	24.442411637271658	23.555307635491577	28.93046872344173	23.07181200379503
40	24.69381700408862	22.612267098500837	30.028802680009026	22.665113217401515
41	22.847911342136896	23.666562622650897	27.830280517088458	25.65524551812375
42	22.93552464452486	24.186679687620718	29.123465222000057	23.75433044585436
43	24.152685108210928	22.208658728417305	28.927841869578252	24.71081429379352
44	24.226700578834976	22.40768153878009	28.734381808573435	24.6312360738115
45	23.621597065340673	22.359162002713383	28.92506049489927	25.094180437046674
46	24.72209431332495	23.47990147752804	28.693279271650685	23.10472493749633
47	23.050333610440664	23.397387362051543	31.009700816797032	22.542578210710765
48	22.54675027272924	23.447143064642237	29.841987014070664	24.164119648557858
49	22.897976086358593	23.211807862637176	30.676862980212068	23.213353070792166
50	22.455737512400294	22.82488774062754	30.604701759374002	24.11467298759816
51	23.01834780163236	23.015720947768877	28.25366755155587	25.712263699042897
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	368.0
1	905.0
2	1442.0
3	6540.5
4	11639.0
5	7450.5
6	3262.0
7	3176.5
8	3091.0
9	3024.5
10	2958.0
11	2918.0
12	2878.0
13	2879.5
14	2881.0
15	2607.5
16	2334.0
17	2384.5
18	2435.0
19	2369.5
20	2304.0
21	2252.5
22	2201.0
23	2423.0
24	2645.0
25	3113.0
26	3944.5
27	4308.0
28	5121.5
29	5935.0
30	7198.5
31	8462.0
32	9105.5
33	9749.0
34	10837.5
35	11926.0
36	12505.0
37	13084.0
38	14357.5
39	15631.0
40	17609.5
41	19588.0
42	22315.0
43	25042.0
44	28564.0
45	32086.0
46	42006.0
47	51926.0
48	53168.0
49	54410.0
50	54382.5
51	54355.0
52	49818.0
53	45281.0
54	42564.5
55	39848.0
56	38515.5
57	37183.0
58	35531.5
59	33880.0
60	32607.5
61	31335.0
62	30250.0
63	29165.0
64	26280.5
65	23396.0
66	20530.0
67	17664.0
68	15249.0
69	12834.0
70	11053.5
71	9273.0
72	7843.0
73	6413.0
74	5529.0
75	3716.5
76	2788.0
77	2091.5
78	1395.0
79	1124.5
80	854.0
81	615.5
82	377.0
83	285.5
84	194.0
85	132.0
86	70.0
87	55.5
88	41.0
89	21.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	647162.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.11627745883853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.57915468197409	23.309722694942398
2	8.01573201139927	5.148709466275856
3	3.123657918329983	3.009607931747512
4	1.8700341936898588	2.4023414808823564
5	1.489480956902098	2.3918291840762045
6	1.2613812729315461	2.430652256571148
7	1.1054784322335809	2.4852696438062862
8	0.9900784376874028	2.54381070486256
9	0.8827912807060755	2.5516772738459763
>10	8.574475873376239	46.018236405061025
>50	0.06135577344359278	1.3567101194706082
>100	0.037199957126292926	2.300076716680186
>500	0.00579739591578591	1.141659773430438
>1k	0.002898697957892955	1.7034904393167678
>5k	4.8311632631549254E-4	1.2062059090307453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7774	1.2012448196896603	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGC	2511	0.38800176771812933	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCG	2444	0.3776488730796926	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTC	2315	0.3577156878803144	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1590	0.24568809664349883	RNA PCR Primer, Index 35 (95% over 24bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1066	0.16471918932199356	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	1053	0.16271041872050584	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGCT	789	0.1219169234287551	TruSeq Adapter, Index 10 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	788	0.12176240261325603	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCC	655	0.1012111341518816	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	649	0.10028400925888727	TruSeq Adapter, Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5452081549905588E-4	0.0	0.0	0.12562542300073243	0.0
2	1.5452081549905588E-4	0.0	0.0	0.6377074055646036	0.0
3	1.5452081549905588E-4	0.0	0.0	0.9153813110164071	0.0
4	1.5452081549905588E-4	0.0	0.0	1.2647528748597723	0.0
5	1.5452081549905588E-4	0.0	0.0	2.3731306844345004	0.0
6	1.5452081549905588E-4	0.0	0.0	3.155006010859723	0.0
7	1.5452081549905588E-4	0.0	0.0	3.8297984121441	0.0
8	1.5452081549905588E-4	0.0	0.0	4.762022492049904	0.0
9	1.5452081549905588E-4	0.0	0.0	5.231765771167034	0.0
10	3.0904163099811175E-4	0.0	0.0	6.418640155015282	0.0
11	3.0904163099811175E-4	0.0	0.0	7.402165145666773	0.0
12	3.0904163099811175E-4	0.0	0.0	8.566788532083157	0.0
13	3.0904163099811175E-4	0.0	0.0	8.934084510524412	0.0
14	3.0904163099811175E-4	0.0	0.0	9.109311115300342	0.0
15	3.0904163099811175E-4	0.0	0.0	9.417734663036457	0.0
16	3.0904163099811175E-4	0.0	0.0	9.876970526699651	0.0
17	3.0904163099811175E-4	0.0	0.0	10.415166527082864	0.0
18	3.0904163099811175E-4	0.0	0.0	11.006981250444248	0.0
19	3.0904163099811175E-4	0.0	0.0	11.449683386849042	0.0
20	3.0904163099811175E-4	0.0	0.0	11.816979365290297	0.0
21	3.0904163099811175E-4	0.0	0.0	12.21749731906385	0.0
22	3.0904163099811175E-4	0.0	0.0	12.64474737391874	0.0
23	3.0904163099811175E-4	0.0	0.0	13.074006199375118	0.0
24	3.0904163099811175E-4	0.0	0.0	13.43048572073144	0.0
25	3.0904163099811175E-4	0.0	0.0	13.750498329629984	0.0
26	3.0904163099811175E-4	0.0	0.0	14.068965730373538	0.0
27	3.0904163099811175E-4	0.0	0.0	14.395159171892045	0.0
28	3.0904163099811175E-4	0.0	0.0	14.73155098723349	0.0
29	3.0904163099811175E-4	0.0	0.0	15.049400304715048	0.0
30	3.0904163099811175E-4	0.0	0.0	15.414378470923818	0.0
31	3.0904163099811175E-4	0.0	0.0	15.760505097641703	0.0
32	3.0904163099811175E-4	0.0	0.0	16.100914454186125	0.0
33	3.0904163099811175E-4	0.0	0.0	16.439778602575554	0.0
34	3.0904163099811175E-4	0.0	0.0	16.75809148250361	0.0
35	3.0904163099811175E-4	0.0	0.0	17.102518380251002	0.0
36	3.0904163099811175E-4	0.0	0.0	17.43412005031198	0.0
37	6.180832619962235E-4	0.0	0.0	17.764021991402462	0.0
38	6.180832619962235E-4	0.0	0.0	18.10504943120888	0.0
39	6.180832619962235E-4	0.0	0.0	18.457047848915728	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	25	3.888909E-5	45.000004	1
CACAACG	50	2.1827873E-11	45.000004	12
GCGTAAG	40	6.8066583E-9	45.0	1
ATCGCAT	30	2.1640262E-6	44.999996	10
AATATAC	170	0.0	42.35294	16
CGAATAT	170	0.0	42.35294	14
CGTAAGG	85	0.0	42.35294	2
ACGGGTA	80	0.0	42.1875	5
CGTTTTT	3240	0.0	42.083332	1
TACGAAT	175	0.0	41.142857	12
ACTACGG	45	1.9261279E-8	40.0	2
CCAATCG	35	6.2440595E-6	38.571426	24
AATCGGT	35	6.2440595E-6	38.571426	26
TAGTAAG	35	6.2440595E-6	38.571426	1
GCTACGA	190	0.0	37.894737	10
AGCTACG	190	0.0	37.894737	9
GACCTAT	155	0.0	37.741936	33
ATACGAA	90	0.0	37.5	19
ATTAGCG	30	1.139371E-4	37.499996	1
CTAAGCG	30	1.139371E-4	37.499996	1
>>END_MODULE