FastQCFastQC Report
Sat 18 Jun 2016
SRR3551507_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551507_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573377
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG33200.5790256672311586No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC30640.5343779049386355RNA PCR Primer, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC30260.5277505027233391No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC28180.49147419586066415Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.4243281471004243No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC18400.3209057914775095RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT16060.2800949462570002RNA PCR Primer, Index 16 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC8110.14144271570014144Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC6520.11371226958876968No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT6250.10900332590948014No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT5950.1037711662658251No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC5830.10167830240836308Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG453.8380676E-1045.0000041
GGTCATA207.030325E-445.00000428
CGTTACC207.030325E-445.00000439
AATTGCG207.030325E-445.0000041
TATACGG207.030325E-445.0000042
TGCGTAG302.1635915E-645.0000041
TACCGGT302.1635915E-645.00000440
TTAATCG253.8883518E-545.020
ATCGTTG253.8883518E-545.023
TATAGCG253.8883518E-545.01
CGTCATA253.8883518E-545.038
TGATTCG501.0786607E-940.515
CGAATAT501.0786607E-940.514
GTAAGCG403.454188E-739.3750041
CGTTTTT12150.039.0740741
GACACGA356.2428135E-638.57142625
CAACCGG356.2428135E-638.5714262
ACTTCGG356.2428135E-638.57142612
CGGTATT356.2428135E-638.57142643
AGCTACG700.038.5714269