Basic Statistics
Measure | Value |
---|---|
Filename | SRR3551507_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 573377 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG | 3320 | 0.5790256672311586 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC | 3064 | 0.5343779049386355 | RNA PCR Primer, Index 16 (95% over 22bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC | 3026 | 0.5277505027233391 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 2818 | 0.49147419586066415 | Illumina PCR Primer Index 7 (95% over 22bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2433 | 0.4243281471004243 | No Hit |
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 1840 | 0.3209057914775095 | RNA PCR Primer, Index 16 (96% over 25bp) |
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT | 1606 | 0.2800949462570002 | RNA PCR Primer, Index 16 (96% over 26bp) |
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 811 | 0.14144271570014144 | Illumina PCR Primer Index 7 (95% over 22bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC | 652 | 0.11371226958876968 | No Hit |
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT | 625 | 0.10900332590948014 | No Hit |
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT | 595 | 0.1037711662658251 | No Hit |
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC | 583 | 0.10167830240836308 | Illumina PCR Primer Index 7 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TTATGCG | 45 | 3.8380676E-10 | 45.000004 | 1 |
GGTCATA | 20 | 7.030325E-4 | 45.000004 | 28 |
CGTTACC | 20 | 7.030325E-4 | 45.000004 | 39 |
AATTGCG | 20 | 7.030325E-4 | 45.000004 | 1 |
TATACGG | 20 | 7.030325E-4 | 45.000004 | 2 |
TGCGTAG | 30 | 2.1635915E-6 | 45.000004 | 1 |
TACCGGT | 30 | 2.1635915E-6 | 45.000004 | 40 |
TTAATCG | 25 | 3.8883518E-5 | 45.0 | 20 |
ATCGTTG | 25 | 3.8883518E-5 | 45.0 | 23 |
TATAGCG | 25 | 3.8883518E-5 | 45.0 | 1 |
CGTCATA | 25 | 3.8883518E-5 | 45.0 | 38 |
TGATTCG | 50 | 1.0786607E-9 | 40.5 | 15 |
CGAATAT | 50 | 1.0786607E-9 | 40.5 | 14 |
GTAAGCG | 40 | 3.454188E-7 | 39.375004 | 1 |
CGTTTTT | 1215 | 0.0 | 39.074074 | 1 |
GACACGA | 35 | 6.2428135E-6 | 38.571426 | 25 |
CAACCGG | 35 | 6.2428135E-6 | 38.571426 | 2 |
ACTTCGG | 35 | 6.2428135E-6 | 38.571426 | 12 |
CGGTATT | 35 | 6.2428135E-6 | 38.571426 | 43 |
AGCTACG | 70 | 0.0 | 38.571426 | 9 |