##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424107
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15895988512333	33.0	31.0	34.0	30.0	34.0
2	32.28695824402804	33.0	31.0	34.0	30.0	34.0
3	32.31940760232677	34.0	31.0	34.0	30.0	34.0
4	35.85023354955236	37.0	35.0	37.0	35.0	37.0
5	35.908933358798606	37.0	35.0	37.0	35.0	37.0
6	35.92475247991663	37.0	35.0	37.0	35.0	37.0
7	36.03000893642406	37.0	35.0	37.0	35.0	37.0
8	35.75188572695098	37.0	35.0	37.0	35.0	37.0
9	37.65563171558121	39.0	37.0	39.0	35.0	39.0
10	37.480690014548216	39.0	37.0	39.0	35.0	39.0
11	37.4228201845289	39.0	37.0	39.0	35.0	39.0
12	37.327506973476034	39.0	37.0	39.0	35.0	39.0
13	37.21228133466319	39.0	37.0	39.0	34.0	39.0
14	38.507746865767366	40.0	38.0	41.0	34.0	41.0
15	38.42794389151794	40.0	38.0	41.0	34.0	41.0
16	38.619360208626595	40.0	38.0	41.0	35.0	41.0
17	38.4335321039266	40.0	38.0	41.0	34.0	41.0
18	38.426793238498774	40.0	38.0	41.0	34.0	41.0
19	38.38055254923875	40.0	37.0	41.0	34.0	41.0
20	38.04078216110557	40.0	36.0	41.0	33.0	41.0
21	38.14248998483873	40.0	37.0	41.0	34.0	41.0
22	38.02664421950121	40.0	37.0	41.0	34.0	41.0
23	37.87754976927992	40.0	36.0	41.0	33.0	41.0
24	37.870829767016346	40.0	36.0	41.0	33.0	41.0
25	37.92871138651331	40.0	36.0	41.0	33.0	41.0
26	37.88416838203567	40.0	36.0	41.0	33.0	41.0
27	37.7102452918721	40.0	36.0	41.0	33.0	41.0
28	37.68364351449045	40.0	36.0	41.0	33.0	41.0
29	37.65138514572973	40.0	36.0	41.0	33.0	41.0
30	37.5187417326288	40.0	36.0	41.0	33.0	41.0
31	37.449219182894886	40.0	35.0	41.0	33.0	41.0
32	37.14767263921605	40.0	35.0	41.0	32.0	41.0
33	37.00465920156942	40.0	35.0	41.0	31.0	41.0
34	36.611739490270146	40.0	35.0	41.0	30.0	41.0
35	36.61596483906184	40.0	35.0	41.0	30.0	41.0
36	36.55593753463159	39.0	35.0	41.0	30.0	41.0
37	36.426340522556806	39.0	35.0	41.0	30.0	41.0
38	36.3009900803335	39.0	35.0	41.0	30.0	41.0
39	36.02954443100444	39.0	35.0	41.0	29.0	41.0
40	36.08173409068938	39.0	35.0	41.0	29.0	41.0
41	35.86493738608417	39.0	35.0	41.0	27.0	41.0
42	35.86479119656124	39.0	35.0	41.0	28.0	41.0
43	35.94664789781824	39.0	35.0	41.0	29.0	41.0
44	36.06349576875647	39.0	35.0	41.0	29.0	41.0
45	35.96025059713704	39.0	35.0	41.0	29.0	41.0
46	35.88675735132879	39.0	35.0	41.0	28.0	41.0
47	35.711261544845996	38.0	35.0	41.0	28.0	41.0
48	35.68014675541785	38.0	35.0	41.0	28.0	41.0
49	35.72471098095528	38.0	35.0	41.0	28.0	41.0
50	35.618480713593506	38.0	35.0	41.0	28.0	41.0
51	34.72635443414044	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	11.0
11	10.0
12	5.0
13	9.0
14	10.0
15	21.0
16	31.0
17	48.0
18	131.0
19	260.0
20	578.0
21	1202.0
22	2128.0
23	2947.0
24	3560.0
25	4184.0
26	4698.0
27	4960.0
28	4793.0
29	4979.0
30	5719.0
31	7265.0
32	9266.0
33	12350.0
34	22407.0
35	31420.0
36	28834.0
37	43512.0
38	82259.0
39	146350.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.13712813040105	17.253428969576074	19.771661396770153	13.837781503252716
2	28.156337905292766	35.14844131315918	21.19205766469311	15.503163116854946
3	27.04647647881313	33.81009980971783	24.765919921151973	14.377503790317068
4	25.133987413553655	20.7763606825636	37.629890570068405	16.459761333814342
5	33.24231856583362	24.04322494087577	23.345287863675914	19.3691686296147
6	22.898466660536137	39.67300704774974	23.848226980455404	13.580299311258715
7	67.51692379517434	5.6122629430780435	21.691695727729087	5.179117534018538
8	66.14722228116962	15.646523165144647	12.134909350706307	6.071345202979437
9	61.10085426555091	7.228836119186903	12.978800161280054	18.691509453982132
10	41.15612333679944	22.50799916058919	20.37033107211152	15.965546430499849
11	32.30929930418503	20.925851259234108	26.500859452921077	20.263989983659783
12	24.6058188145916	17.910574454088238	31.39042741572292	26.093179315597244
13	25.920109783615924	20.445076360446773	35.8628836590766	17.771930196860698
14	20.600697465498094	28.923125532000178	27.924792564140656	22.551384438361076
15	17.087904703294214	22.225287486412626	37.40942733791236	23.27738047238079
16	18.8954674174206	22.65277394619754	28.66658649821861	29.785172138163247
17	19.469379189685622	26.166981445720065	31.337846345379823	23.02579301921449
18	19.567939222884792	22.942795096520456	30.74129877601643	26.747966904578323
19	19.836267734321762	24.601810392188764	27.998830483816583	27.563091389672888
20	23.675629027580303	23.864024880513643	32.873543704772615	19.586802387133435
21	22.020150575208614	29.65265840931652	28.51803907976053	19.809151935714336
22	19.803021407333528	21.537489359996417	31.75330753795622	26.906181694713833
23	22.035005317054424	27.111318605917845	29.05304557576272	21.80063050126501
24	22.33893805101071	24.877448379772087	27.032093316073535	25.75152025314366
25	18.74880631538739	32.62195625160631	25.469280158073314	23.159957274932978
26	18.76436842589252	22.223401169987763	33.04496270988218	25.967267694237538
27	24.88051364396249	24.335368197176656	26.34028676725449	24.443831391606363
28	19.013126404421527	26.00287191675686	31.33171581699901	23.652285861822605
29	23.185894125774865	20.753489095912116	31.244473682349028	24.81614309596399
30	21.56248305262587	27.067933328145966	28.423251679411095	22.946331939817075
31	26.878594317000193	21.265152426156604	25.05546949236867	26.800783764474534
32	30.552902923083092	24.95478735319153	24.059965999146442	20.43234372457894
33	27.324943941033748	22.34766226447571	23.390795247425768	26.936598547064772
34	24.970113674143555	21.1772029228473	30.194738591911946	23.657944811097202
35	27.73993355450393	20.86619650229777	25.070795813320697	26.3230741298776
36	25.64753706022301	26.465962599061086	27.000733305510167	20.88576703520574
37	24.535081948659183	22.724218180789283	28.595849632286193	24.144850238265345
38	28.212220029379374	24.40539769444975	22.773733986941973	24.6086482892289
39	26.863503785601274	23.50703949710804	28.676961238555364	20.95249547873532
40	26.94485118142356	20.786971212453462	28.914401318535184	23.353776287587802
41	21.228369255871748	25.773212892029605	27.68122195578003	25.317195896318616
42	25.05947791477151	22.44127071705961	29.791066876990946	22.708184491177935
43	24.98261052045828	24.459157712558387	25.54402544640857	25.01420632057476
44	23.46318264022994	21.010263919246793	29.756170023130956	25.770383417392306
45	24.215587104197763	19.151534872095958	28.036556812313872	28.596321211392407
46	28.767976006055072	23.776546956310554	26.626063705621462	20.829413332012912
47	21.05081972238138	22.943974044285994	34.70327063689116	21.301935596441464
48	22.663384476087405	24.12362917848562	27.86443043854499	25.34855590688199
49	23.290348897801735	20.226971023821818	33.74643663037865	22.736243447997794
50	24.27642080889964	20.70067223601591	29.855909004095665	25.166997950988783
51	22.278104346308833	19.95321935266336	28.434333788407173	29.334342512620637
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	537.5
2	871.0
3	2702.5
4	4534.0
5	2998.5
6	1463.0
7	1427.0
8	1391.0
9	1339.5
10	1288.0
11	1240.0
12	1192.0
13	1249.0
14	1306.0
15	1209.0
16	1112.0
17	1050.5
18	989.0
19	964.0
20	939.0
21	1016.5
22	1094.0
23	1273.5
24	1453.0
25	1945.5
26	2655.5
27	2873.0
28	3578.0
29	4283.0
30	4756.0
31	5229.0
32	6003.5
33	6778.0
34	7207.5
35	7637.0
36	8308.5
37	8980.0
38	9352.0
39	9724.0
40	11420.0
41	13116.0
42	14317.5
43	15519.0
44	17440.0
45	19361.0
46	21302.5
47	23244.0
48	46629.5
49	70015.0
50	55839.0
51	41663.0
52	41107.0
53	40551.0
54	34836.5
55	29122.0
56	26514.5
57	23907.0
58	21263.5
59	18620.0
60	17401.5
61	16183.0
62	14831.5
63	13480.0
64	12231.5
65	10983.0
66	9099.5
67	7216.0
68	6364.5
69	5513.0
70	4702.0
71	3891.0
72	3205.5
73	2520.0
74	2079.5
75	1234.5
76	830.0
77	650.0
78	470.0
79	381.0
80	292.0
81	209.0
82	126.0
83	89.0
84	52.0
85	29.5
86	7.0
87	8.0
88	9.0
89	4.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	424107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.982948783255598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0643118272551	23.368121430549163
2	8.62576391942297	5.517547313027178
3	3.3404189628259964	3.2050934580803894
4	2.050099338511836	2.6227288857684083
5	1.4904040772148055	2.3833758633958224
6	1.19491726922288	2.293018669306981
7	1.0834828382217638	2.4257083285668157
8	0.9692455887885352	2.479946561969611
9	0.8752469455682114	2.51936804095281
>10	7.173795502636962	36.579715781681166
>50	0.07317910225006923	1.6399933227965857
>100	0.047307702464691216	3.041446460685898
>500	0.002956731404043201	0.5023765043884596
>1k	0.006652645659097202	4.306489601854203
>5k	7.391828510108003E-4	2.085630833748231
>10k+	0.0014783657020216005	5.029438943228278
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCG	10639	2.5085650555166503	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGC	10635	2.507621897304218	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTC	8822	2.080135437519305	No Hit
GCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	3556	0.8384676508522614	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2857	0.6736507532297275	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGGGTGAT	2341	0.5519833438259685	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCT	2227	0.5251033347716496	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTA	1644	0.38763802530965064	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCT	1579	0.3723117043576267	No Hit
CCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	1529	0.3605222267022237	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTC	1337	0.3152506325054762	No Hit
CTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGCT	1146	0.27021482786183676	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	568	0.1339284661653781	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATG	527	0.12426109448794762	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTT	517	0.12190319895686702	No Hit
TCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTGC	513	0.12096004074443477	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCC	497	0.11718740789470582	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGGGTGATCGTATGCCGTCTTCTG	457	0.10775582577038341	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGGGTGA	431	0.10162529738957386	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.7157910621612E-4	0.0	0.0	0.2801179890923753	0.0
2	4.7157910621612E-4	0.0	0.0	1.717491104839109	0.0
3	7.0736865932418E-4	0.0	0.0	2.1782238916122583	0.0
4	7.0736865932418E-4	0.0	0.0	3.0152768051458714	0.0
5	7.0736865932418E-4	0.0	0.0	6.282376853011151	0.0
6	7.0736865932418E-4	0.0	0.0	7.091135020171796	0.0
7	7.0736865932418E-4	0.0	0.0	8.318655433652356	0.0
8	7.0736865932418E-4	0.0	0.0	9.588853756245475	0.0
9	7.0736865932418E-4	0.0	0.0	9.982858099489045	0.0
10	7.0736865932418E-4	0.0	0.0	13.348282391000385	0.0
11	7.0736865932418E-4	0.0	0.0	14.607634394150534	0.0
12	7.0736865932418E-4	0.0	0.0	18.246338777714115	0.0
13	7.0736865932418E-4	0.0	0.0	18.823315814169536	0.0
14	7.0736865932418E-4	0.0	0.0	19.086456955438134	0.0
15	7.0736865932418E-4	0.0	0.0	19.787459296828395	0.0
16	7.0736865932418E-4	0.0	0.0	20.45875215452704	0.0
17	7.0736865932418E-4	0.0	0.0	21.202196615476755	0.0
18	7.0736865932418E-4	0.0	0.0	21.956487395869438	0.0
19	7.0736865932418E-4	0.0	0.0	23.046306710334893	0.0
20	7.0736865932418E-4	0.0	0.0	23.61314479600667	0.0
21	7.0736865932418E-4	0.0	0.0	24.130231285972645	0.0
22	7.0736865932418E-4	0.0	0.0	24.759553603218055	0.0
23	7.0736865932418E-4	0.0	0.0	25.267680090165925	0.0
24	7.0736865932418E-4	0.0	0.0	25.717330767942997	0.0
25	7.0736865932418E-4	0.0	0.0	26.088463524535083	0.0
26	7.0736865932418E-4	0.0	0.0	26.45488049006501	0.0
27	7.0736865932418E-4	0.0	0.0	26.90311643052343	0.0
28	7.0736865932418E-4	0.0	0.0	27.258687076610382	0.0
29	7.0736865932418E-4	0.0	0.0	27.621802988396794	0.0
30	7.0736865932418E-4	0.0	0.0	28.029247336167522	0.0
31	7.0736865932418E-4	0.0	0.0	28.393542195719476	0.0
32	7.0736865932418E-4	0.0	0.0	28.753592843315484	0.0
33	7.0736865932418E-4	0.0	0.0	29.10774875208379	0.0
34	7.0736865932418E-4	0.0	0.0	29.460725713086557	0.0
35	7.0736865932418E-4	0.0	0.0	29.81370267408932	0.0
36	7.0736865932418E-4	0.0	0.0	30.157012263414657	0.0
37	7.0736865932418E-4	0.0	0.0	30.513290278160937	0.0
38	7.0736865932418E-4	0.0	0.0	30.855656709273838	0.0
39	7.0736865932418E-4	0.0	0.0	31.193778928430795	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAATCG	30	2.162251E-6	45.000004	20
ATAGCGG	35	1.2095006E-7	45.000004	2
AATGCGG	30	2.162251E-6	45.000004	2
CACTAGG	30	2.162251E-6	45.000004	2
CGAGACA	30	2.162251E-6	45.000004	22
ATAACGC	30	2.162251E-6	45.000004	11
CGCCGAT	75	0.0	45.000004	38
GTCGATA	20	7.028248E-4	45.0	19
ATTAGCG	20	7.028248E-4	45.0	1
TTTCGAG	25	3.886629E-5	45.0	1
TTTCGAC	20	7.028248E-4	45.0	24
CGCATCG	20	7.028248E-4	45.0	21
TCGATTA	20	7.028248E-4	45.0	41
TAACGCC	20	7.028248E-4	45.0	12
CTAACGG	25	3.886629E-5	45.0	2
GTCGTTT	25	3.886629E-5	45.0	9
CATACGG	20	7.028248E-4	45.0	2
CGTAAGC	20	7.028248E-4	45.0	43
CCGGTGT	20	7.028248E-4	45.0	33
TTGCCCG	20	7.028248E-4	45.0	24
>>END_MODULE