FastQCFastQC Report
Sat 18 Jun 2016
SRR3551504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG68291.5147079046947294No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC66001.4639145073927682No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC56921.2625153600120662No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.6396862786849611No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC23070.5117046619022904No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT15920.3531139236014071No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT12350.2739294570651619No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC10360.22979021661498605No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA10260.22757216433105765No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT9330.20694427809052315No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT7870.17456071474516796Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC7770.17234266246123953No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC5150.1142296926223145No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5150.1142296926223145No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG302.1625547E-645.0000041
GTCGAAT1400.045.043
CCGATCG253.8870214E-545.040
ACGTTAG253.8870214E-545.01
TTTCGAT207.0287206E-445.043
TGGAACG253.8870214E-545.01
TAAGTAG253.8870214E-545.01
CTAAGCG207.0287206E-445.01
GCGTTGC207.0287206E-445.040
ACGGGTA253.8870214E-545.05
GGTATGA207.0287206E-445.08
TTAACGG351.2097371E-745.02
CGGTCTA207.0287206E-445.031
CGATCGA253.8870214E-545.041
GTACGAG207.0287206E-445.01
GCGAGTA253.8870214E-545.027
CGTAAGG650.044.9999962
GGTCGAA1450.043.4482842
CGGCGAT950.042.6315831
CGGTCGA1450.041.89655341