##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551504_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450846
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29090421119407	33.0	31.0	34.0	30.0	34.0
2	32.41907879852544	34.0	31.0	34.0	30.0	34.0
3	32.434536404892135	34.0	31.0	34.0	30.0	34.0
4	35.97404878827804	37.0	35.0	37.0	35.0	37.0
5	36.02382853568624	37.0	35.0	37.0	35.0	37.0
6	36.037425196186724	37.0	35.0	37.0	35.0	37.0
7	36.12446378586036	37.0	36.0	37.0	35.0	37.0
8	35.93383993647498	37.0	35.0	37.0	35.0	37.0
9	37.800938679726556	39.0	38.0	39.0	35.0	39.0
10	37.59659174086052	39.0	37.0	39.0	35.0	39.0
11	37.557829059146584	39.0	37.0	39.0	35.0	39.0
12	37.475980711817336	39.0	37.0	39.0	35.0	39.0
13	37.38404244464851	39.0	37.0	39.0	35.0	39.0
14	38.723448805135234	40.0	38.0	41.0	35.0	41.0
15	38.70094222861021	40.0	38.0	41.0	35.0	41.0
16	38.83483717278184	40.0	38.0	41.0	35.0	41.0
17	38.694980104071014	40.0	38.0	41.0	35.0	41.0
18	38.65589580477591	40.0	38.0	41.0	35.0	41.0
19	38.5660247623357	40.0	38.0	41.0	35.0	41.0
20	38.30966227935925	40.0	37.0	41.0	34.0	41.0
21	38.340544221308384	40.0	37.0	41.0	34.0	41.0
22	38.29970987876126	40.0	37.0	41.0	34.0	41.0
23	38.169179719904356	40.0	37.0	41.0	34.0	41.0
24	38.13354670996305	40.0	37.0	41.0	34.0	41.0
25	38.12535100677393	40.0	37.0	41.0	34.0	41.0
26	38.04015340049595	40.0	36.0	41.0	34.0	41.0
27	37.940254543680105	40.0	36.0	41.0	34.0	41.0
28	37.8652732862219	40.0	36.0	41.0	33.0	41.0
29	37.76566721230753	40.0	36.0	41.0	33.0	41.0
30	37.68201558847145	40.0	36.0	41.0	33.0	41.0
31	37.61257280756622	40.0	36.0	41.0	33.0	41.0
32	37.3467525496511	40.0	36.0	41.0	33.0	41.0
33	37.24112889989043	40.0	36.0	41.0	33.0	41.0
34	36.96221104323871	40.0	35.0	41.0	31.0	41.0
35	36.94128593799213	40.0	35.0	41.0	31.0	41.0
36	36.8182949388483	40.0	35.0	41.0	31.0	41.0
37	36.65341158621791	40.0	35.0	41.0	31.0	41.0
38	36.54882598492612	39.0	35.0	41.0	30.0	41.0
39	36.51327947902388	39.0	35.0	41.0	30.0	41.0
40	36.4582518199119	39.0	35.0	41.0	30.0	41.0
41	36.38548417863306	39.0	35.0	41.0	30.0	41.0
42	36.271443020454875	39.0	35.0	41.0	30.0	41.0
43	36.22416523602294	39.0	35.0	41.0	30.0	41.0
44	36.271465200977715	39.0	35.0	41.0	30.0	41.0
45	36.18253904881046	39.0	35.0	41.0	30.0	41.0
46	36.14924608402869	39.0	35.0	41.0	30.0	41.0
47	35.99866695057736	39.0	35.0	41.0	30.0	41.0
48	36.01850964630938	39.0	35.0	41.0	30.0	41.0
49	35.992234598953964	39.0	35.0	41.0	30.0	41.0
50	35.843243590937924	38.0	35.0	41.0	29.0	41.0
51	35.07683333111528	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	9.0
10	8.0
11	16.0
12	9.0
13	15.0
14	17.0
15	16.0
16	18.0
17	57.0
18	121.0
19	235.0
20	547.0
21	1256.0
22	1832.0
23	2580.0
24	3090.0
25	3851.0
26	4661.0
27	4935.0
28	4975.0
29	4945.0
30	5454.0
31	6891.0
32	8731.0
33	11947.0
34	22200.0
35	32349.0
36	30341.0
37	44152.0
38	82797.0
39	172534.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.226769229404276	17.181254796538063	20.661600635250174	14.930375338807487
2	31.020792022109546	30.07811980143996	22.326027069110076	16.57506110734042
3	29.17714696370823	29.629407824401238	25.666192003477907	15.527253208412628
4	27.024305416927284	21.99886435723063	32.934527532683	18.042302693159083
5	29.72012616281391	25.89287694689539	24.16656685431389	20.220430035976808
6	24.489071656397083	37.22335342888702	24.033483717278184	14.254091197437708
7	71.19326776770782	5.954361356205888	17.616436654644822	5.235934221441467
8	70.90225930805641	12.118550458471407	11.42075120994752	5.558439023524662
9	65.38130536813014	7.370587739494196	12.938786193068145	14.309320699307523
10	39.93270429370561	22.975029167387532	20.359280108950728	16.732986429956124
11	31.674452030183254	22.908265793641288	25.639575376070766	19.777706800104692
12	26.276821797243404	19.923432835158792	30.289056573641552	23.51068879395625
13	25.665526587792726	21.588746489932262	33.50390155396743	19.241825368307584
14	20.700860160675706	28.980405726123777	28.039507947281333	22.279226165919184
15	17.739094945946064	23.450801382290184	36.21547047106994	22.594633200693806
16	20.348633457987873	23.10034912142949	30.09830407722371	26.45271334335893
17	20.70662709661392	25.209938648673823	29.979194669576753	24.104239585135502
18	21.125838978276395	23.528211406999286	30.32964693043744	25.016302684286874
19	22.538294672682024	24.671839164592786	27.825244096653844	24.96462206607134
20	26.346468638958758	23.56614010105446	30.391308783930654	19.696082476056127
21	23.306627983834836	28.55631412943666	27.25675729628299	20.88030059044552
22	21.760645541936714	22.966600568708607	30.012243648607285	25.260510240747397
23	23.920806661254616	26.535003083092672	27.707465520377244	21.83672473527546
24	23.087262612954312	24.738380733110642	27.581036540193328	24.593320113741722
25	21.325907294286743	30.201887118883164	26.059230868190024	22.41297471864007
26	20.538942343948932	25.921933431814853	29.09463541874609	24.44448880549012
27	25.304205870740788	25.34058192819721	26.537221135376605	22.8179910656854
28	19.86310181303594	26.950887886329255	30.804088313969736	22.38192198666507
29	22.844829498320934	25.55883827293577	28.940258979784673	22.656073248958624
30	23.853599677051587	27.034952067890146	28.211185194057393	20.900263061000874
31	23.386921476513045	24.291886808355848	27.13986594091996	25.18132577421115
32	26.55474374841964	26.743943608238734	26.954658575211937	19.746654068129693
33	26.968854109829078	24.26016866069567	24.9783739902317	23.792603239243558
34	23.12363867041074	25.47455228614649	28.462934128283273	22.9388749151595
35	27.716559534741354	23.359195822963937	25.975166686629137	22.94907795566557
36	25.127870714168477	27.952116687294552	26.83510555710819	20.08490704142878
37	24.231112175776207	24.520346193600474	30.4704932504669	20.778048380156417
38	24.494616787106906	24.08072823092586	27.380081003269407	24.044573978697827
39	24.433842154527266	24.411883436916376	29.633400318512308	21.52087409004405
40	26.4970743890375	22.618588165360233	29.184022925788405	21.70031451981386
41	23.160014727867164	24.79205759838171	28.260204149532214	23.78772352421891
42	24.906952706689204	24.340018542917093	28.90499194847021	21.848036801923495
43	26.168359040559302	24.077179347271574	26.53367225172232	23.220789360446805
44	24.064092838796398	23.276462472773407	29.474144164526244	23.185300523903948
45	22.945307266782894	22.630343842465052	28.084312603416688	26.340036287335366
46	25.98403889576485	25.759793809859687	26.767454962448372	21.488712331927086
47	22.32114735408543	24.87856163745492	32.14290467254894	20.65738633591071
48	23.679482572763206	23.867795211668728	28.576498405220406	23.876223810347657
49	23.110108551478774	22.41319652386846	31.92021222324253	22.55648270141024
50	23.667061479973206	22.570456430798988	29.292929292929294	24.46955279629851
51	22.160560368729012	22.56779476805827	27.78931164965421	27.48233321355851
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	616.5
2	1044.0
3	2953.0
4	4862.0
5	3295.0
6	1728.0
7	1638.0
8	1548.0
9	1558.5
10	1569.0
11	1526.0
12	1483.0
13	1451.5
14	1420.0
15	1365.5
16	1311.0
17	1297.5
18	1284.0
19	1335.0
20	1386.0
21	1448.5
22	1511.0
23	1598.5
24	1686.0
25	2157.5
26	3066.5
27	3504.0
28	3966.5
29	4429.0
30	5574.0
31	6719.0
32	7010.5
33	7302.0
34	8195.0
35	9088.0
36	10084.0
37	11080.0
38	12185.5
39	13291.0
40	14903.0
41	16515.0
42	18478.5
43	20442.0
44	22893.5
45	25345.0
46	40409.5
47	55474.0
48	48198.5
49	40923.0
50	40120.5
51	39318.0
52	34185.5
53	29053.0
54	26947.0
55	24841.0
56	23377.0
57	21913.0
58	20967.0
59	20021.0
60	19336.5
61	18652.0
62	17473.5
63	16295.0
64	14835.0
65	13375.0
66	11229.5
67	9084.0
68	7562.0
69	6040.0
70	5571.0
71	5102.0
72	4447.5
73	3793.0
74	3078.0
75	1925.0
76	1487.0
77	1205.5
78	924.0
79	661.5
80	399.0
81	335.0
82	271.0
83	178.5
84	86.0
85	62.5
86	39.0
87	30.0
88	21.0
89	13.5
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	450846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.312051098959756
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.44443596085195	23.40828316602366
2	8.249658501435395	5.3312677409469655
3	3.28623855520678	3.185553243576395
4	1.9644976664853815	2.539077959330514
5	1.5776970840789863	2.5489314399720007
6	1.3118495753994595	2.5433130308673597
7	1.118481134157441	2.5298293692082385
8	1.032439800831365	2.6688198080850327
9	0.967874436608672	2.8146607427768666
>10	7.930358907283916	41.31885825437406
>50	0.07236200978914721	1.603535796386329
>100	0.034458099899593905	2.2199206157721587
>500	0.0034458099899593907	0.7854007434876522
>1k	0.004134971987951269	2.2446383596131367
>5k	0.0020674859939756344	4.257909729579642
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	6829	1.5147079046947294	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	6600	1.4639145073927682	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	5692	1.2625153600120662	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2884	0.6396862786849611	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2307	0.5117046619022904	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	1592	0.3531139236014071	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	1235	0.2739294570651619	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1036	0.22979021661498605	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	1026	0.22757216433105765	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	933	0.20694427809052315	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	787	0.17456071474516796	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	777	0.17234266246123953	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	515	0.1142296926223145	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	515	0.1142296926223145	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.4361045678568734E-4	0.0	0.0	0.17810959839945348	0.0
2	4.4361045678568734E-4	0.0	0.0	1.0804132675015417	0.0
3	4.4361045678568734E-4	0.0	0.0	1.3882789245108085	0.0
4	4.4361045678568734E-4	0.0	0.0	1.9179498099129193	0.0
5	4.4361045678568734E-4	0.0	0.0	4.100735062526894	0.0
6	4.4361045678568734E-4	0.0	0.0	4.851323955408277	0.0
7	4.4361045678568734E-4	0.0	0.0	5.704386863807153	0.0
8	4.4361045678568734E-4	0.0	0.0	6.68143889487763	0.0
9	4.4361045678568734E-4	0.0	0.0	7.053849873349215	0.0
10	4.4361045678568734E-4	0.0	0.0	9.312936124530328	0.0
11	4.4361045678568734E-4	0.0	0.0	10.325476992143658	0.0
12	4.4361045678568734E-4	0.0	0.0	12.602085856367806	0.0
13	4.4361045678568734E-4	0.0	0.0	13.008211229555103	0.0
14	4.4361045678568734E-4	0.0	0.0	13.1974110893742	0.0
15	4.4361045678568734E-4	0.0	0.0	13.682499123869349	0.0
16	4.4361045678568734E-4	0.0	0.0	14.253425781752528	0.0
17	4.4361045678568734E-4	0.0	0.0	14.870931537598205	0.0
18	4.4361045678568734E-4	0.0	0.0	15.544110405770486	0.0
19	6.65415685178531E-4	0.0	0.0	16.298691792762938	0.0
20	6.65415685178531E-4	0.0	0.0	16.75760681030773	0.0
21	6.65415685178531E-4	0.0	0.0	17.203213514148956	0.0
22	6.65415685178531E-4	0.0	0.0	17.711812902853747	0.0
23	6.65415685178531E-4	0.0	0.0	18.192464832781038	0.0
24	6.65415685178531E-4	0.0	0.0	18.589052581147442	0.0
25	6.65415685178531E-4	0.0	0.0	18.938617621094565	0.0
26	6.65415685178531E-4	0.0	0.0	19.282637530331865	0.0
27	6.65415685178531E-4	0.0	0.0	19.710056205444875	0.0
28	6.65415685178531E-4	0.0	0.0	20.04742195783039	0.0
29	6.65415685178531E-4	0.0	0.0	20.38035160564805	0.0
30	6.65415685178531E-4	0.0	0.0	20.804443202335165	0.0
31	6.65415685178531E-4	0.0	0.0	21.14402700700461	0.0
32	6.65415685178531E-4	0.0	0.0	21.497362735834407	0.0
33	6.65415685178531E-4	0.0	0.0	21.816762264720104	0.0
34	6.65415685178531E-4	0.0	0.0	22.13483096223544	0.0
35	6.65415685178531E-4	0.0	0.0	22.505023888423096	0.0
36	6.65415685178531E-4	0.0	0.0	22.843498666950577	0.0
37	6.65415685178531E-4	0.0	0.0	23.18463510821877	0.0
38	6.65415685178531E-4	0.0	0.0	23.50669629984518	0.0
39	6.65415685178531E-4	0.0	0.0	23.83896053197766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	30	2.1625547E-6	45.000004	1
GTCGAAT	140	0.0	45.0	43
CCGATCG	25	3.8870214E-5	45.0	40
ACGTTAG	25	3.8870214E-5	45.0	1
TTTCGAT	20	7.0287206E-4	45.0	43
TGGAACG	25	3.8870214E-5	45.0	1
TAAGTAG	25	3.8870214E-5	45.0	1
CTAAGCG	20	7.0287206E-4	45.0	1
GCGTTGC	20	7.0287206E-4	45.0	40
ACGGGTA	25	3.8870214E-5	45.0	5
GGTATGA	20	7.0287206E-4	45.0	8
TTAACGG	35	1.2097371E-7	45.0	2
CGGTCTA	20	7.0287206E-4	45.0	31
CGATCGA	25	3.8870214E-5	45.0	41
GTACGAG	20	7.0287206E-4	45.0	1
GCGAGTA	25	3.8870214E-5	45.0	27
CGTAAGG	65	0.0	44.999996	2
GGTCGAA	145	0.0	43.44828	42
CGGCGAT	95	0.0	42.63158	31
CGGTCGA	145	0.0	41.896553	41
>>END_MODULE