##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551499_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	464449
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25918238601009	33.0	31.0	34.0	30.0	34.0
2	32.377044627074234	34.0	31.0	34.0	30.0	34.0
3	32.40937971661044	34.0	31.0	34.0	30.0	34.0
4	35.944028300200884	37.0	35.0	37.0	35.0	37.0
5	36.007621934808775	37.0	35.0	37.0	35.0	37.0
6	36.01284963472846	37.0	35.0	37.0	35.0	37.0
7	36.1234215166789	37.0	36.0	37.0	35.0	37.0
8	35.957556157941994	37.0	35.0	37.0	35.0	37.0
9	37.83201169557906	39.0	38.0	39.0	35.0	39.0
10	37.58189596704913	39.0	37.0	39.0	35.0	39.0
11	37.54481116333548	39.0	37.0	39.0	35.0	39.0
12	37.452217573942455	39.0	37.0	39.0	35.0	39.0
13	37.38318523669983	39.0	37.0	39.0	35.0	39.0
14	38.657878475354664	40.0	38.0	41.0	35.0	41.0
15	38.664342048319625	40.0	38.0	41.0	35.0	41.0
16	38.76976804772968	40.0	38.0	41.0	35.0	41.0
17	38.66510208871157	40.0	38.0	41.0	35.0	41.0
18	38.62256351074068	40.0	38.0	41.0	35.0	41.0
19	38.57867279292237	40.0	38.0	41.0	35.0	41.0
20	38.41850235440275	40.0	37.0	41.0	34.0	41.0
21	38.39197199261921	40.0	37.0	41.0	34.0	41.0
22	38.35846131652776	40.0	37.0	41.0	34.0	41.0
23	38.29482677323021	40.0	37.0	41.0	34.0	41.0
24	38.20741136271151	40.0	37.0	41.0	34.0	41.0
25	38.16808519342274	40.0	37.0	41.0	34.0	41.0
26	38.15607311028767	40.0	37.0	41.0	34.0	41.0
27	38.10405663485119	40.0	37.0	41.0	34.0	41.0
28	38.028334650306064	40.0	37.0	41.0	34.0	41.0
29	38.01441277729094	40.0	37.0	41.0	34.0	41.0
30	37.88799200773389	40.0	36.0	41.0	33.0	41.0
31	37.900800733772705	40.0	36.0	41.0	34.0	41.0
32	37.73078421958062	40.0	36.0	41.0	33.0	41.0
33	37.630807688249945	40.0	36.0	41.0	33.0	41.0
34	37.54610947595969	40.0	36.0	41.0	33.0	41.0
35	37.4328053241583	40.0	36.0	41.0	33.0	41.0
36	37.36516388236383	40.0	36.0	41.0	33.0	41.0
37	37.29015457025422	40.0	36.0	41.0	33.0	41.0
38	37.11704406727111	40.0	35.0	41.0	32.0	41.0
39	37.11596752280659	40.0	35.0	41.0	32.0	41.0
40	37.015239563439685	40.0	35.0	41.0	32.0	41.0
41	36.978910493940134	39.0	35.0	41.0	32.0	41.0
42	36.90813846084285	39.0	35.0	41.0	32.0	41.0
43	36.876567717876455	39.0	35.0	41.0	32.0	41.0
44	36.90719756098086	39.0	35.0	41.0	32.0	41.0
45	36.79436278256601	39.0	35.0	41.0	32.0	41.0
46	36.71784200202821	39.0	35.0	41.0	32.0	41.0
47	36.62123936104933	39.0	35.0	41.0	31.0	41.0
48	36.57571875491173	39.0	35.0	41.0	31.0	41.0
49	36.57912709468639	39.0	35.0	41.0	32.0	41.0
50	36.47927113633575	39.0	35.0	41.0	31.0	41.0
51	35.72629287607466	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	10.0
11	19.0
12	14.0
13	10.0
14	16.0
15	21.0
16	32.0
17	45.0
18	108.0
19	209.0
20	453.0
21	791.0
22	1269.0
23	1738.0
24	2231.0
25	2805.0
26	3432.0
27	3947.0
28	4153.0
29	4530.0
30	5312.0
31	6590.0
32	8907.0
33	12089.0
34	23642.0
35	34289.0
36	30337.0
37	46562.0
38	86239.0
39	184368.0
40	264.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.241890928821036	18.867087667321925	22.02566912621192	12.865352277645123
2	30.241641170505268	29.13280037205377	22.83910612360022	17.786452333840742
3	30.4117351958988	28.04290675617775	25.585801670366394	15.959556377557064
4	28.048935405179037	23.437449536978225	29.96884480319691	18.54477025464583
5	28.775387609834446	26.657609339238537	23.531324214284023	21.03567883664299
6	24.677844068993583	36.33832778195238	23.756106698474966	15.227721450579073
7	73.67332042915369	6.363669638647085	14.625502477128812	5.3375074550704165
8	72.6527562767925	10.338271801640223	10.694392710502122	6.314579211065155
9	66.58922723485247	7.942960368092083	12.710975801433527	12.756836595621909
10	39.49970825645012	23.638548042949818	20.40525439822241	16.456489302377655
11	30.58290576575684	22.406981175543493	25.388794033359957	21.6213190253397
12	25.77591942279992	20.07475524761599	30.627690015480706	23.521635314103378
13	24.586983716188428	22.49009040820413	32.774104368832745	20.148821506774695
14	19.994014412777293	28.034725018247432	28.863233638138958	23.108026930836324
15	19.05634418418384	23.425176930082745	35.165755551201535	22.352723334531884
16	20.69613671253464	23.470607106485318	30.080805427506576	25.75245075347347
17	20.352934337246932	25.125686566232247	29.331745789096324	25.189633307424497
18	21.264767498692	24.09026610026074	29.610786114298882	25.03418028674838
19	22.690973605282817	25.5726678278993	27.09920787858301	24.637150688234875
20	24.771503437406476	24.77580961526454	29.582795958221464	20.869890989107525
21	23.358215864389848	27.69604412971069	27.97680692605647	20.968933079842998
22	21.257016378547483	23.531324214284023	27.970347659269372	27.241311747899122
23	22.646835282237664	26.609810765014025	27.607552174727473	23.135801778020838
24	22.94654526115892	24.434545020012962	28.047212934035816	24.5716967847923
25	21.50935840103004	28.797133808017673	25.449941758944473	24.24356603200782
26	20.79840843666366	24.45930554269683	28.567183910397056	26.17510211024246
27	24.13246664326977	24.462965793876183	27.463295216482326	23.941272346371722
28	19.867197474857303	25.186618982923854	30.454151047800725	24.49203249441812
29	22.18973450260416	22.521740815460902	28.52067718952996	26.76784749240498
30	21.195868652962975	26.539189448141776	29.51669612810018	22.748245770795073
31	22.357890747961562	24.366076792069745	26.57600726882822	26.70002519114047
32	22.739848723971846	25.698408221354768	27.539729873462964	24.022013181210426
33	21.244097844973293	25.243891148436106	27.17392006442042	26.338090942170183
34	19.834255214243115	23.33022570831243	28.12149450208742	28.714024575357033
35	20.833719095099784	22.67956223395895	28.633068431625432	27.853650239315837
36	20.268102633443068	26.053883203537954	28.661704514381558	25.01630964863742
37	20.106405654872763	24.188662264317504	31.97982986291283	23.725102217896907
38	19.597846049835397	26.891650105824322	28.292665071945468	25.217838772394817
39	21.578257246759065	23.596132191047886	29.508299081276956	25.317311480916093
40	20.78699706533979	23.901440201184627	28.803377765911865	26.508184967563714
41	18.771705827765807	24.00500377867107	27.84697566363584	29.376314729927287
42	19.57394676272314	23.317737792524046	30.43821818972589	26.670097255026924
43	22.013827137102243	22.83286216570603	29.778727050763376	25.37458364642835
44	22.023946655068695	22.628964644126697	29.539303561855014	25.807785138949598
45	21.300724083806834	22.06205632911256	28.275225051620307	28.3619945354603
46	23.301374316663402	24.39815781711232	27.41528133336491	24.885186532859365
47	19.159477143884473	23.722949128967873	32.83568271220306	24.28189101494459
48	20.395780806934667	23.391588742789843	29.114068498371186	27.098561951904298
49	20.34496790820951	22.09973538537062	32.49226502802245	25.06303167839741
50	20.64381665155916	22.11114675669449	30.9764904219839	26.268546169762452
51	20.69075399021206	21.552850797396488	27.834918365633254	29.9214768467582
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	458.5
2	789.0
3	2104.0
4	3419.0
5	2348.5
6	1278.0
7	1243.5
8	1209.0
9	1133.5
10	1058.0
11	1070.0
12	1082.0
13	1055.5
14	1029.0
15	1008.5
16	988.0
17	988.5
18	989.0
19	1022.0
20	1055.0
21	1129.0
22	1203.0
23	1282.5
24	1362.0
25	1858.0
26	2938.5
27	3523.0
28	4208.5
29	4894.0
30	5590.0
31	6286.0
32	6998.5
33	7711.0
34	8918.0
35	10125.0
36	10524.5
37	10924.0
38	12094.5
39	13265.0
40	15410.0
41	17555.0
42	20645.5
43	23736.0
44	38531.5
45	53327.0
46	48230.5
47	43134.0
48	43487.0
49	43840.0
50	38491.0
51	33142.0
52	30397.0
53	27652.0
54	26214.5
55	24777.0
56	23667.5
57	22558.0
58	21191.0
59	19824.0
60	19514.5
61	19205.0
62	17723.5
63	16242.0
64	15149.5
65	14057.0
66	12126.0
67	10195.0
68	8701.0
69	7207.0
70	5891.0
71	4575.0
72	4064.0
73	3553.0
74	2985.5
75	1961.0
76	1504.0
77	1110.5
78	717.0
79	520.5
80	324.0
81	260.0
82	196.0
83	108.5
84	21.0
85	16.5
86	12.0
87	7.0
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	464449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.3066911184621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.83189878962766	23.206509666491318
2	9.115748432269072	5.889993378298439
3	3.378929909478147	3.274861346893307
4	2.0412704390678815	2.6378677425685417
5	1.478857871982782	2.3888502239126947
6	1.2359608895055265	2.3957884015052824
7	1.0503699504949346	2.375378428552792
8	0.9516406924904625	2.4595489526438996
9	0.886108461625763	2.576450913047923
>10	7.89018602757789	41.981339589569345
>50	0.08421920923493195	1.840888485787659
>100	0.044114823884964356	2.5264979804945
>500	0.0060156578024951396	1.4986235884300707
>1k	0.0026736256899978395	1.5191378882716928
>5k	0.0020052192674983797	3.428263413532537
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	5397	1.1620220949985898	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC	5335	1.1486729436385912	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	5144	1.1075489450940792	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	2671	0.5750900529444568	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2046	0.4405219948799545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	1246	0.2682748805573917	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	1072	0.23081113319223423	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTACCTT	944	0.2032515949006242	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	926	0.19937603482836652	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	870	0.18731873682578712	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	833	0.17935230778836858	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTACCTTCGTA	799	0.17203180542965968	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTC	762	0.16406537639224114	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	671	0.1444722671380496	No Hit
TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	612	0.1317690424567606	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC	523	0.11260655098837546	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2045434482580434	0.0
2	0.0	0.0	0.0	1.2526671389108384	0.0
3	0.0	0.0	0.0	1.645821177352088	0.0
4	0.0	0.0	0.0	2.3029439184926654	0.0
5	0.0	0.0	0.0	4.675863227178872	0.0
6	0.0	0.0	0.0	5.604921100056195	0.0
7	0.0	0.0	0.0	6.681465564572213	0.0
8	0.0	0.0	0.0	8.098413388768197	0.0
9	0.0	0.0	0.0	8.619676218486852	0.0
10	0.0	0.0	0.0	10.789128623379531	0.0
11	0.0	0.0	0.0	12.085072849763915	0.0
12	0.0	0.0	0.0	14.25021907679853	0.0
13	0.0	0.0	0.0	14.758778681835896	0.0
14	0.0	0.0	0.0	14.97279572138168	0.0
15	0.0	0.0	0.0	15.483723724240983	0.0
16	0.0	0.0	0.0	16.161946736886073	0.0
17	0.0	0.0	0.0	16.928446395621478	0.0
18	0.0	0.0	0.0	17.754801926583973	0.0
19	0.0	0.0	0.0	18.449173106196806	0.0
20	0.0	0.0	0.0	18.96031641794901	0.0
21	0.0	0.0	0.0	19.506985697030245	0.0
22	0.0	0.0	0.0	20.130089633092116	0.0
23	0.0	0.0	0.0	20.72584933975528	0.0
24	0.0	0.0	0.0	21.194792108498458	0.0
25	0.0	0.0	0.0	21.631223234413252	0.0
26	0.0	0.0	0.0	22.02566912621192	0.0
27	0.0	0.0	0.0	22.465545194413163	0.0
28	0.0	0.0	0.0	22.88195259328796	0.0
29	0.0	0.0	0.0	23.27919750069437	0.0
30	0.0	0.0	0.0	23.74297285600787	0.0
31	0.0	0.0	0.0	24.130959481019445	0.0
32	0.0	0.0	0.0	24.5447831731794	0.0
33	0.0	0.0	0.0	24.96872638330581	0.0
34	0.0	0.0	0.0	25.374153028642542	0.0
35	0.0	0.0	0.0	25.78410116073024	0.0
36	0.0	0.0	0.0	26.167996916776655	0.0
37	0.0	0.0	0.0	26.554261070645	0.0
38	2.1530889290320357E-4	0.0	0.0	26.944185475692702	0.0
39	2.1530889290320357E-4	0.0	0.0	27.326789378381694	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACACGT	20	7.028939E-4	45.000004	10
TAGCCGT	20	7.028939E-4	45.000004	44
GTATGCG	40	6.7993824E-9	45.000004	1
TAGTACG	20	7.028939E-4	45.000004	1
TATCCTC	20	7.028939E-4	45.000004	42
CCATTCG	20	7.028939E-4	45.000004	35
TCGATTA	25	3.8872036E-5	45.0	41
ATCGCAT	25	3.8872036E-5	45.0	10
TACGGCT	725	0.0	41.89655	7
CGTTTTT	1055	0.0	41.587677	1
ACGGCTG	750	0.0	40.499996	8
CATCGAA	45	1.924127E-8	40.000004	42
TGAATGA	495	0.0	40.0	22
GTTACCG	40	3.4522964E-7	39.375004	38
ATGAATG	510	0.0	39.264706	21
GAATGAC	155	0.0	39.193546	1
CGAATAT	115	0.0	39.130436	14
CGTATGG	70	0.0	38.57143	2
GGATCGA	35	6.2402487E-6	38.57143	8
GATGAAT	520	0.0	38.509613	20
>>END_MODULE