##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	648708
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33576431923146	33.0	31.0	34.0	30.0	34.0
2	32.473911220456664	34.0	31.0	34.0	31.0	34.0
3	32.4853817125733	34.0	31.0	34.0	31.0	34.0
4	36.01198073709589	37.0	35.0	37.0	35.0	37.0
5	36.06477644795501	37.0	35.0	37.0	35.0	37.0
6	36.0742922855892	37.0	35.0	37.0	35.0	37.0
7	36.1627326932919	37.0	36.0	37.0	35.0	37.0
8	36.02989018171504	37.0	36.0	37.0	35.0	37.0
9	37.888096030879844	39.0	38.0	39.0	35.0	39.0
10	37.68136665495107	39.0	37.0	39.0	35.0	39.0
11	37.6148328677926	39.0	37.0	39.0	35.0	39.0
12	37.475082779925636	39.0	37.0	39.0	35.0	39.0
13	37.387715274052425	39.0	37.0	39.0	35.0	39.0
14	38.66643235477287	40.0	38.0	41.0	35.0	41.0
15	38.70106889386288	40.0	38.0	41.0	35.0	41.0
16	38.78447776195145	40.0	38.0	41.0	35.0	41.0
17	38.70446795784852	40.0	38.0	41.0	35.0	41.0
18	38.63207791487079	40.0	38.0	41.0	35.0	41.0
19	38.52951713251571	40.0	37.0	41.0	35.0	41.0
20	38.32830641829606	40.0	37.0	41.0	34.0	41.0
21	38.26873261929867	40.0	37.0	41.0	34.0	41.0
22	38.2532865326156	40.0	37.0	41.0	34.0	41.0
23	38.17050352392756	40.0	36.0	41.0	34.0	41.0
24	38.113482491352045	40.0	36.0	41.0	34.0	41.0
25	38.04178613490199	40.0	36.0	41.0	34.0	41.0
26	38.00904413079537	40.0	36.0	41.0	34.0	41.0
27	37.9716482608508	40.0	36.0	41.0	34.0	41.0
28	37.88821318682674	40.0	36.0	41.0	34.0	41.0
29	37.83407326562953	40.0	35.0	41.0	34.0	41.0
30	37.695363707554094	40.0	35.0	41.0	33.0	41.0
31	37.65729573244048	40.0	35.0	41.0	33.0	41.0
32	37.41051443792893	40.0	35.0	41.0	33.0	41.0
33	37.25351159535569	40.0	35.0	41.0	33.0	41.0
34	37.058471608181186	40.0	35.0	41.0	32.0	41.0
35	36.926853067944286	40.0	35.0	41.0	32.0	41.0
36	36.80792282506151	40.0	35.0	41.0	31.0	41.0
37	36.705940114812826	40.0	35.0	41.0	31.0	41.0
38	36.612742559055846	39.0	35.0	41.0	31.0	41.0
39	36.54791369922985	39.0	35.0	41.0	31.0	41.0
40	36.49742102764264	39.0	35.0	41.0	31.0	41.0
41	36.4079477977765	39.0	35.0	41.0	30.0	41.0
42	36.28528243832356	39.0	35.0	41.0	30.0	41.0
43	36.2050984418259	39.0	35.0	41.0	30.0	41.0
44	36.21228195120146	39.0	35.0	41.0	30.0	41.0
45	36.10352731891699	39.0	35.0	41.0	30.0	41.0
46	36.04738341441758	39.0	35.0	41.0	30.0	41.0
47	35.95426601799269	38.0	35.0	41.0	30.0	41.0
48	35.90015076120535	38.0	35.0	41.0	30.0	41.0
49	35.85390653421879	38.0	35.0	41.0	30.0	41.0
50	35.77462433020712	38.0	35.0	41.0	30.0	41.0
51	34.991772877781685	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	15.0
10	14.0
11	15.0
12	17.0
13	23.0
14	21.0
15	35.0
16	55.0
17	92.0
18	163.0
19	299.0
20	582.0
21	1110.0
22	1826.0
23	2603.0
24	3627.0
25	4813.0
26	6459.0
27	7540.0
28	7658.0
29	7563.0
30	8102.0
31	9957.0
32	12664.0
33	18005.0
34	36364.0
35	50477.0
36	45006.0
37	66515.0
38	119077.0
39	237733.0
40	272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.537603975902876	18.376989338808833	21.84742596052461	15.237980724763684
2	32.90586827971907	26.725583775751183	23.1893240101864	17.17922393434334
3	30.9834316826677	25.445655055895717	26.631396560548044	16.939516700888536
4	28.343723215992405	23.49824574384777	29.808635009896594	18.349396030263232
5	27.391522842326594	27.409096234361225	25.2904234262565	19.908957497055685
6	25.31061741184015	34.97706209881796	25.05056204023998	14.661758449101908
7	73.39157217114634	6.7327672851267435	14.839188047626974	5.0364724960999405
8	73.81055883386671	9.088372580575543	11.778180629805707	5.322887955752049
9	67.70534662745025	7.968300067210516	13.820239614741917	10.50611369059731
10	41.033716248296614	23.004803393822797	19.97308496272591	15.988395395154676
11	32.277850743323654	23.346867928251232	25.11253137004631	19.26274995837881
12	27.450563273460478	20.855916683623448	30.273559136005723	21.41996090691035
13	26.307059570715946	21.668917294067594	32.82015945540983	19.20386367980663
14	21.757555017049274	26.0537869118309	30.45438009088835	21.734277980231475
15	20.526184354131598	22.701276999821182	34.33393761137523	22.438601034671997
16	23.463561417463634	22.951312454910376	30.065299025139197	23.51982710248679
17	24.13304599295831	23.69509856514796	29.191870610505806	22.979984831387927
18	24.73347022080813	22.4217984054459	29.419399791585736	23.425331582160233
19	25.061506872121203	24.071384968275403	27.64263736534774	23.22447079425566
20	26.8509098084192	24.281957367567532	28.854584805490298	20.01254801852297
21	25.222287993981883	26.07305598204431	28.675305376224742	20.029350647749062
22	23.577788465688723	22.42981433865468	29.853339252791706	24.139057942864895
23	23.924477576968375	24.842610234496874	29.309026557403328	21.92388563113142
24	23.86543714583449	23.932339357615444	28.931969391467348	23.270254105082717
25	23.54402905467483	26.003995634399452	27.640325076922128	22.81165023400359
26	22.00296589528725	23.658872713146746	30.029843935946527	24.308317455619477
27	24.777249548332993	23.52028956017191	28.76872182861935	22.933739062875745
28	21.406703786603526	25.172959174235558	30.168118783797947	23.252218255362965
29	23.108393915290083	24.150311079869525	29.53131455138521	23.209980453455177
30	22.60770639486487	25.09310814727119	29.768709496414413	22.53047596144953
31	24.39125153381799	23.414386750279014	26.725737928312892	25.4686237875901
32	24.68707029973424	25.887456297748756	26.693982500601194	22.73149090191581
33	24.46817366210992	23.78681933936378	25.975014952798485	25.769992045727818
34	22.361678906380067	23.92062376292569	28.20113209641318	25.516565234281064
35	23.110552051153984	23.809942223619874	27.87463697071718	25.204868754508965
36	23.63050864179261	25.866491549356567	26.461212132423217	24.041787676427607
37	22.480992989141495	26.599641132836343	27.055470257804743	23.863895620217416
38	22.63314156754657	26.036675977481394	24.97471897988001	26.355463475092026
39	24.16588048860196	25.177892056210187	25.37150767371452	25.28471978147333
40	23.336077248931723	22.6591933504751	27.915024941884486	26.089704458708695
41	22.27566177694741	23.75938018337989	26.55185383870709	27.41310420096561
42	22.273811946206923	23.778032643346467	28.30163956664632	25.646515843800294
43	23.78126984714232	23.111168661400818	28.01584071724104	25.091720774215826
44	23.084191963102043	22.741048360741658	28.844102431294203	25.330657244862092
45	22.612330971716087	22.81550404804627	27.417882930378536	27.154282049859102
46	23.4543122637612	24.58039672703281	27.07196458190742	24.893326427298568
47	21.54266634602934	24.125492517434655	30.394877202069342	23.93696393446666
48	21.814437312319257	24.473260696646257	28.34696041978826	25.36534157124623
49	22.299092966326914	23.356271234515376	30.026606732150675	24.318029067007036
50	22.12551718184453	22.969810762315248	29.08673856342145	25.817933492418778
51	21.436917688698152	23.18670341663738	27.756864413572824	27.619514481091645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	288.0
1	783.0
2	1278.0
3	5891.0
4	10504.0
5	6729.0
6	2954.0
7	2862.0
8	2770.0
9	2740.5
10	2711.0
11	2660.5
12	2610.0
13	2524.0
14	2438.0
15	2343.5
16	2249.0
17	2226.0
18	2203.0
19	2152.0
20	2101.0
21	2261.0
22	2421.0
23	2452.5
24	2484.0
25	3023.5
26	4085.5
27	4608.0
28	5664.5
29	6721.0
30	7539.5
31	8358.0
32	9221.0
33	10084.0
34	10961.5
35	11839.0
36	13048.0
37	14257.0
38	15594.5
39	16932.0
40	18636.5
41	20341.0
42	22348.5
43	24356.0
44	27108.5
45	29861.0
46	32989.5
47	36118.0
48	49248.5
49	62379.0
50	58442.5
51	54506.0
52	54491.0
53	54476.0
54	49440.5
55	44405.0
56	42133.0
57	39861.0
58	37677.5
59	35494.0
60	33979.0
61	32464.0
62	30100.0
63	27736.0
64	25597.5
65	23459.0
66	19690.5
67	15922.0
68	13837.5
69	11753.0
70	9973.0
71	8193.0
72	6947.5
73	5702.0
74	4675.0
75	2950.0
76	2252.0
77	1715.5
78	1179.0
79	920.0
80	661.0
81	518.0
82	375.0
83	256.0
84	137.0
85	91.0
86	45.0
87	25.0
88	5.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	648708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.569163505285687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.1510306625093	22.77747684059637
2	8.299432503444617	5.240122834046513
3	3.2849013209316014	3.1110476069765594
4	2.017593837998798	2.547749990361638
5	1.4747532802154415	2.3278363716538837
6	1.2553888624649558	2.377894575712248
7	1.1018852494652307	2.4349916923101262
8	0.984883517795178	2.4873559045549536
9	0.8890555638477271	2.526006641435357
>10	8.42778375638943	45.69843840562327
>50	0.06375709022508505	1.4090854316897878
>100	0.04070644991236904	2.2663655146532906
>500	0.005885269866848536	1.2517806521363475
>1k	0.0024521957778535566	2.505574062278604
>5k	4.904391555707113E-4	1.0382734759711003
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6706	1.0337470788089558	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCG	4794	0.7390073808246546	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGC	4209	0.6488281322259013	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTC	4191	0.6460533861151705	No Hit
GCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	1932	0.2978227492184465	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTCACCCGT	1057	0.16293925772458487	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCT	967	0.14906552717093052	No Hit
GAACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	901	0.13889145809825065	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCT	856	0.13195459282142352	No Hit
CCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGC	747	0.11515196359533103	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTCACCCGTCGTA	649	0.10004501254801852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15353595146044138	0.0
2	0.0	0.0	0.0	0.6727217792905282	0.0
3	0.0	0.0	0.0	0.9108874871282611	0.0
4	0.0	0.0	0.0	1.317233639788626	0.0
5	0.0	0.0	0.0	2.6602107573823663	0.0
6	0.0	0.0	0.0	3.3706999142911758	0.0
7	0.0	0.0	0.0	4.025848301547075	0.0
8	0.0	0.0	0.0	4.885865443311937	0.0
9	0.0	0.0	0.0	5.292211595972302	0.0
10	0.0	0.0	0.0	6.71041516367919	0.0
11	0.0	0.0	0.0	7.596329935810873	0.0
12	0.0	0.0	0.0	8.964125615839484	0.0
13	0.0	0.0	0.0	9.312818710421329	0.0
14	1.5415256170727044E-4	0.0	0.0	9.485932037218594	0.0
15	3.083051234145409E-4	0.0	0.0	9.809498264242155	0.0
16	3.083051234145409E-4	0.0	0.0	10.274884848036404	0.0
17	3.083051234145409E-4	0.0	0.0	10.77773050432552	0.0
18	3.083051234145409E-4	0.0	0.0	11.341158117365595	0.0
19	3.083051234145409E-4	0.0	0.0	11.851094791493246	0.0
20	3.083051234145409E-4	0.0	0.0	12.231235008663374	0.0
21	3.083051234145409E-4	0.0	0.0	12.633110737034228	0.0
22	3.083051234145409E-4	0.0	0.0	13.090018929934578	0.0
23	3.083051234145409E-4	0.0	0.0	13.497289997965186	0.0
24	3.083051234145409E-4	0.0	0.0	13.82347681853777	0.0
25	3.083051234145409E-4	0.0	0.0	14.13471084062475	0.0
26	3.083051234145409E-4	0.0	0.0	14.431454521911245	0.0
27	3.083051234145409E-4	0.0	0.0	14.772748293531142	0.0
28	3.083051234145409E-4	0.0	0.0	15.087373671975682	0.0
29	3.083051234145409E-4	0.0	0.0	15.408627610573632	0.0
30	3.083051234145409E-4	0.0	0.0	15.808653508204	0.0
31	3.083051234145409E-4	0.0	0.0	16.140389820998045	0.0
32	3.083051234145409E-4	0.0	0.0	16.485845711784037	0.0
33	3.083051234145409E-4	0.0	0.0	16.827756093650763	0.0
34	4.6245768512181133E-4	0.0	0.0	17.1550219821553	0.0
35	4.6245768512181133E-4	0.0	0.0	17.490766261553734	0.0
36	4.6245768512181133E-4	0.0	0.0	17.82142350641583	0.0
37	4.6245768512181133E-4	0.0	0.0	18.165029566461335	0.0
38	4.6245768512181133E-4	0.0	0.0	18.52004291607318	0.0
39	6.166102468290818E-4	0.0	0.0	18.976026193603285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATGCG	20	7.031012E-4	45.0	1
TTACGAG	20	7.031012E-4	45.0	1
TGCGTTG	25	3.88892E-5	45.0	1
TGCGTCG	20	7.031012E-4	45.0	1
CGATCAC	75	0.0	42.0	34
CGATGAA	620	0.0	41.37097	19
CGTTTTT	2850	0.0	40.657894	1
CAAACGA	50	1.0804797E-9	40.5	15
CGTCGAA	95	0.0	40.263157	42
ATTAGCG	40	3.4551158E-7	39.375	1
TCGATTT	40	3.4551158E-7	39.375	14
TACGAAA	80	0.0	39.375	20
ATACGAA	80	0.0	39.375	19
ACATACG	80	0.0	39.375	17
GATGAAT	710	0.0	38.661972	20
ATGAATG	715	0.0	38.39161	21
CCGTCGA	100	0.0	38.25	41
CCGATGA	645	0.0	37.67442	18
CTAGACG	30	1.1393739E-4	37.499996	17
CTCACGA	60	1.546141E-10	37.499996	24
>>END_MODULE