FastQCFastQC Report
Sat 18 Jun 2016
SRR3551497_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551497_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401549
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25800.6425118727726877No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10860.2704526720275732No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC9300.23160311692968977No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG8930.22238879937442252No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC8640.21516676669596987No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC8580.21367255303835894No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA6270.15614532722033925No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC5130.12775526772573212No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT5070.1262610540681212No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4560.11356023797842853No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA4080.10160652871754132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG207.0277974E-445.01
TCGATGG253.886257E-545.02
TAGTAAG207.0277974E-445.01
CCATTAC750.045.06
CGAATAT1800.043.7514
GCTACGA1800.043.7510
CTACGAA1850.042.56756611
AGCTACG1850.042.5675669
ACGGGTA650.041.538465
TACGAAT1900.041.44737212
AAGCTAC1850.041.3513538
ATAGGGT603.6379788E-1241.2499964
ATCGGGA556.002665E-1140.9090924
CGTTTTT10500.040.7142831
TAAGTAG451.9228537E-840.01
TAGCGAG403.450732E-739.3751
GTTCACG403.450732E-739.3751
AATACGG403.450732E-739.3752
GACCTAT1850.038.9189233
GTGTTAG356.2381387E-638.5714261