##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551497_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 401549 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.328216481674716 33.0 31.0 34.0 30.0 34.0 2 32.45163603943728 34.0 31.0 34.0 31.0 34.0 3 32.466593118149966 34.0 31.0 34.0 31.0 34.0 4 35.98548371431631 37.0 35.0 37.0 35.0 37.0 5 36.05686479109648 37.0 35.0 37.0 35.0 37.0 6 36.06520250330595 37.0 35.0 37.0 35.0 37.0 7 36.17155814109859 37.0 37.0 37.0 35.0 37.0 8 36.061307586371775 37.0 37.0 37.0 35.0 37.0 9 37.905289267312334 39.0 38.0 39.0 35.0 39.0 10 37.633927615309716 39.0 37.0 39.0 35.0 39.0 11 37.57713006382782 39.0 37.0 39.0 35.0 39.0 12 37.516233386211894 39.0 37.0 39.0 35.0 39.0 13 37.47963262266872 39.0 37.0 39.0 35.0 39.0 14 38.79009535573492 40.0 38.0 41.0 35.0 41.0 15 38.86156110462235 40.0 38.0 41.0 35.0 41.0 16 38.89173176872561 40.0 38.0 41.0 35.0 41.0 17 38.829208888578975 40.0 38.0 41.0 35.0 41.0 18 38.78467385051388 40.0 38.0 41.0 35.0 41.0 19 38.69767326029949 40.0 38.0 41.0 35.0 41.0 20 38.59382043038334 40.0 38.0 41.0 35.0 41.0 21 38.49543642245405 40.0 38.0 41.0 35.0 41.0 22 38.484117753997644 40.0 38.0 41.0 35.0 41.0 23 38.43968731088859 40.0 37.0 41.0 35.0 41.0 24 38.35322214723483 40.0 37.0 41.0 34.0 41.0 25 38.235308766800564 40.0 37.0 41.0 34.0 41.0 26 38.23166288547599 40.0 37.0 41.0 34.0 41.0 27 38.21026076518682 40.0 37.0 41.0 34.0 41.0 28 38.13961434345497 40.0 37.0 41.0 34.0 41.0 29 38.06658963165143 40.0 37.0 41.0 34.0 41.0 30 37.94355109837156 40.0 36.0 41.0 34.0 41.0 31 37.91176419316198 40.0 36.0 41.0 34.0 41.0 32 37.77854757451768 40.0 36.0 41.0 33.0 41.0 33 37.683784544351994 40.0 36.0 41.0 33.0 41.0 34 37.52857061031157 40.0 36.0 41.0 33.0 41.0 35 37.42970098294355 40.0 36.0 41.0 33.0 41.0 36 37.33257460484275 40.0 35.0 41.0 33.0 41.0 37 37.240545985670494 40.0 35.0 41.0 33.0 41.0 38 37.09998032618684 40.0 35.0 41.0 32.0 41.0 39 37.05825939051025 40.0 35.0 41.0 32.0 41.0 40 36.978642706120546 39.0 35.0 41.0 32.0 41.0 41 36.8921103028522 39.0 35.0 41.0 32.0 41.0 42 36.80606102866649 39.0 35.0 41.0 31.0 41.0 43 36.76232539490822 39.0 35.0 41.0 31.0 41.0 44 36.71785759645772 39.0 35.0 41.0 31.0 41.0 45 36.59068756241455 39.0 35.0 41.0 31.0 41.0 46 36.49975719028064 39.0 35.0 41.0 31.0 41.0 47 36.42643612610167 39.0 35.0 41.0 31.0 41.0 48 36.35872334385094 39.0 35.0 41.0 31.0 41.0 49 36.269336992496555 38.0 35.0 41.0 31.0 41.0 50 36.14344700148674 38.0 35.0 40.0 31.0 41.0 51 35.29787398300083 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 7.0 9 8.0 10 9.0 11 10.0 12 7.0 13 16.0 14 25.0 15 29.0 16 30.0 17 60.0 18 87.0 19 158.0 20 311.0 21 424.0 22 718.0 23 1047.0 24 1467.0 25 2147.0 26 3108.0 27 3699.0 28 4024.0 29 4227.0 30 4734.0 31 5577.0 32 7429.0 33 10429.0 34 20475.0 35 29629.0 36 28269.0 37 43343.0 38 78117.0 39 151706.0 40 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.579859494109066 19.890972210116324 23.684780686790404 13.84438760898421 2 32.09147576011894 25.84541363569577 24.676191448615235 17.386919155570055 3 32.19980625029573 24.430393301938246 26.636599767400742 16.733200680365286 4 28.939930120607947 25.06667928447089 26.974541089630407 19.01884950529076 5 25.97864768683274 29.9891171438604 23.930329797857798 20.101905371449064 6 26.140271797464322 35.26045389230206 23.55951577516069 15.03975853507293 7 78.18448059888084 6.687602260247193 10.558113704678632 4.569803436193341 8 78.81653297605024 7.4665856470816765 9.080087361691849 4.63679401517623 9 72.10452522606208 8.599448635160343 11.684003695688446 7.6120224430891374 10 39.79613944997995 25.9460240219749 18.52401574901195 15.733820779033195 11 29.685791771365388 24.10764315189429 25.64170250704148 20.564862569698843 12 26.392046798771755 21.90118765082219 29.91241417610304 21.794351374303012 13 24.17488276648678 23.850887438394818 30.926736214011243 21.04749358110716 14 20.965311829938564 26.88912187553698 29.299039469653763 22.84652682487069 15 20.606949587721548 24.977026465014234 32.5514445310535 21.864579416210724 16 22.17089321602096 23.658880983391814 30.654291257106852 23.51593454348037 17 22.118595738004576 25.04401704399712 28.64108738908577 24.196299828912537 18 23.19891221245726 24.076762736303664 29.48008835783429 23.24423669340479 19 24.34597023028323 25.416574315961437 27.46414509810758 22.773310355647755 20 25.25918381069309 25.024343230838575 27.971928706085684 21.744544252382646 21 24.744676241255736 26.099429957489622 27.25072158067882 21.90517222057582 22 23.685029722400007 24.135037068950492 26.60098767522768 25.578945533421827 23 23.326169408963786 25.36029226819143 28.207516392769 23.106021930075784 24 23.16454529833221 24.754388630030206 29.286587689173675 22.794478382463907 25 23.12544670762472 27.06294873103905 26.62041245277662 23.1911921085596 26 22.805934020505592 26.153470684773218 26.792994130230678 24.247601164490508 27 23.915138625672085 25.787388338658545 26.93120889355969 23.36626414210968 28 21.831955751352886 26.75165421903678 27.93731275635103 23.479077273259303 29 23.402374305501944 25.03181429912663 27.15036023000929 24.415451165362136 30 24.465756358501704 26.26777802958045 26.639339159106363 22.62712645281149 31 24.696363332992984 26.942166460382172 24.075766593865254 24.285703612759587 32 24.760614520270252 27.362289533780437 24.660751240819927 23.216344705129384 33 24.502364593113168 26.37710466219565 24.69686140421219 24.423669340478995 34 23.026081499393598 26.37884791146286 25.74604842746464 24.8490221616789 35 24.85748937240536 25.40735999840617 25.43699523594879 24.29815539323968 36 24.5479381096703 28.048382638233445 24.44782579461037 22.955853457485887 37 23.073647300827545 29.10753108586997 25.94029620295406 21.878525410348427 38 22.153709758958435 28.04016446311658 26.07153796921422 23.734587808710767 39 23.204889067087702 27.138157485138798 26.875673952618484 22.78127949515501 40 23.83669240864751 25.402130250604532 27.967944136332058 22.7932332044159 41 21.324670214593986 26.01724820632102 27.32767358404578 25.330407995039213 42 21.597364207107976 25.562260147578503 27.49627069174621 25.34410495356731 43 23.240501159260763 24.17114723234275 28.019245471910025 24.569106136486457 44 22.92746339799128 25.05621978886761 26.880405629200922 25.13591118394019 45 22.990967478439742 25.897960149321754 25.891734259081705 25.2193381131568 46 23.373735210397733 26.94814331501261 26.63386037569512 23.04426109889453 47 22.35069692615347 25.624768085588556 29.15136135315989 22.87317363509808 48 21.915382680569493 25.826735964975633 27.944783824639085 24.31309752981579 49 21.8065541191735 25.362782624287444 29.092339913684256 23.738323342854795 50 22.080742325345103 24.830593526568364 29.227317214088444 23.861346933998092 51 21.938792027872065 24.721266893953167 27.59389265071012 25.74604842746464 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 489.5 2 783.0 3 2454.0 4 4125.0 5 2738.5 6 1352.0 7 1289.0 8 1226.0 9 1222.0 10 1218.0 11 1191.5 12 1165.0 13 1138.5 14 1112.0 15 1108.0 16 1104.0 17 1015.5 18 927.0 19 958.0 20 989.0 21 1087.5 22 1186.0 23 1427.5 24 1669.0 25 2059.5 26 3142.5 27 3835.0 28 4557.5 29 5280.0 30 6031.5 31 6783.0 32 7464.5 33 8146.0 34 9654.5 35 11163.0 36 11509.0 37 11855.0 38 12426.0 39 12997.0 40 14546.0 41 16095.0 42 16574.0 43 17053.0 44 18972.5 45 20892.0 46 22840.5 47 24789.0 48 28360.5 49 31932.0 50 32554.5 51 33177.0 52 32119.5 53 31062.0 54 28365.0 55 25668.0 56 24367.0 57 23066.0 58 21174.5 59 19283.0 60 19308.0 61 19333.0 62 17644.0 63 15955.0 64 14916.5 65 13878.0 66 11537.5 67 9197.0 68 8202.0 69 7207.0 70 5884.0 71 4561.0 72 4015.0 73 3469.0 74 2966.5 75 1976.5 76 1489.0 77 1124.5 78 760.0 79 559.5 80 359.0 81 273.5 82 188.0 83 122.0 84 56.0 85 39.5 86 23.0 87 25.5 88 28.0 89 16.0 90 4.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 401549.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.29301638513931 #Duplication Level Percentage of deduplicated Percentage of total 1 69.41246699964509 22.415379341524808 2 7.65756701878809 4.945718744160525 3 3.5340233633577864 3.423728231351344 4 2.5003027879878976 3.229692756012107 5 1.9988519193547245 3.2274478891594636 6 1.7872396841823786 3.462921624328366 7 1.591915440811988 3.598542598972846 8 1.4384621581609927 3.716182563431664 9 1.2999458301986966 3.778125479496003 >10 8.668643723872194 42.2641681882563 >50 0.068049891470871 1.492826336810076 >100 0.0355715341779553 2.2332462245052707 >500 0.005413059548819285 1.2965463935627157 >1k 0.0015465884425197955 0.9154736284285278 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2580 0.6425118727726877 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1086 0.2704526720275732 No Hit GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC 930 0.23160311692968977 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG 893 0.22238879937442252 No Hit GAATCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTC 864 0.21516676669596987 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGC 858 0.21367255303835894 No Hit CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA 627 0.15614532722033925 No Hit AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC 513 0.12775526772573212 No Hit CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT 507 0.1262610540681212 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 456 0.11356023797842853 No Hit GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA 408 0.10160652871754132 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.13149080186975937 0.0 2 0.0 0.0 0.0 0.5785097211050209 0.0 3 0.0 0.0 0.0 0.7949216658489997 0.0 4 0.0 0.0 0.0 1.0880365783503383 0.0 5 0.0 0.0 0.0 1.9907906631569248 0.0 6 0.0 0.0 0.0 2.575526274501991 0.0 7 0.0 0.0 0.0 3.1017385175906305 0.0 8 0.0 0.0 0.0 3.9487086258464097 0.0 9 2.49035609601817E-4 0.0 0.0 4.31603615000909 0.0 10 2.49035609601817E-4 0.0 0.0 5.147317014859955 0.0 11 2.49035609601817E-4 0.0 0.0 5.90463430365908 0.0 12 2.49035609601817E-4 0.0 0.0 6.744880450455611 0.0 13 2.49035609601817E-4 0.0 0.0 7.006367840537519 0.0 14 2.49035609601817E-4 0.0 0.0 7.117686758029531 0.0 15 2.49035609601817E-4 0.0 0.0 7.360994548610506 0.0 16 2.49035609601817E-4 0.0 0.0 7.747995885931729 0.0 17 2.49035609601817E-4 0.0 0.0 8.23461146709368 0.0 18 2.49035609601817E-4 0.0 0.0 8.753601677503866 0.0 19 2.49035609601817E-4 0.0 0.0 9.097519854363975 0.0 20 2.49035609601817E-4 0.0 0.0 9.393125122961333 0.0 21 2.49035609601817E-4 0.0 0.0 9.729821267142988 0.0 22 2.49035609601817E-4 0.0 0.0 10.090673865456022 0.0 23 2.49035609601817E-4 0.0 0.0 10.488881805209326 0.0 24 2.49035609601817E-4 0.0 0.0 10.802666673307616 0.0 25 2.49035609601817E-4 0.0 0.0 11.08980473117851 0.0 26 2.49035609601817E-4 0.0 0.0 11.345564302239577 0.0 27 4.98071219203634E-4 0.0 0.0 11.61751118792476 0.0 28 7.471068288054509E-4 0.0 0.0 11.894936857021186 0.0 29 7.471068288054509E-4 0.0 0.0 12.164393386610351 0.0 30 0.0012451780480090848 0.0 0.0 12.47942343275665 0.0 31 0.0014942136576109018 0.0 0.0 12.767059561846748 0.0 32 0.0014942136576109018 0.0 0.0 13.045232337771978 0.0 33 0.0014942136576109018 0.0 0.0 13.307715870292293 0.0 34 0.0014942136576109018 0.0 0.0 13.594355856943984 0.0 35 0.0014942136576109018 0.0 0.0 13.925822253324002 0.0 36 0.0014942136576109018 0.0 0.0 14.244089762395125 0.0 37 0.0014942136576109018 0.0 0.0 14.530729749046817 0.0 38 0.0014942136576109018 0.0 0.0 14.82583694642497 0.0 39 0.0014942136576109018 0.0 0.0 15.109239470151836 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTCG 20 7.0277974E-4 45.0 1 TCGATGG 25 3.886257E-5 45.0 2 TAGTAAG 20 7.0277974E-4 45.0 1 CCATTAC 75 0.0 45.0 6 CGAATAT 180 0.0 43.75 14 GCTACGA 180 0.0 43.75 10 CTACGAA 185 0.0 42.567566 11 AGCTACG 185 0.0 42.567566 9 ACGGGTA 65 0.0 41.53846 5 TACGAAT 190 0.0 41.447372 12 AAGCTAC 185 0.0 41.351353 8 ATAGGGT 60 3.6379788E-12 41.249996 4 ATCGGGA 55 6.002665E-11 40.909092 4 CGTTTTT 1050 0.0 40.714283 1 TAAGTAG 45 1.9228537E-8 40.0 1 TAGCGAG 40 3.450732E-7 39.375 1 GTTCACG 40 3.450732E-7 39.375 1 AATACGG 40 3.450732E-7 39.375 2 GACCTAT 185 0.0 38.91892 33 GTGTTAG 35 6.2381387E-6 38.571426 1 >>END_MODULE