FastQCFastQC Report
Sat 18 Jun 2016
SRR3551495_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551495_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences494373
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC34650.7008877912021895No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG33920.6861216126285213No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC33670.6810647021580871No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.5760832407918718No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC21940.44379446288531127TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT10110.2045014594243618TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC9750.1972195083469364TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC7490.15150503769421064No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC7470.1511004848565759TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT6740.13633430628290785No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT6170.12480455041031771No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT5990.12116357487160505No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA5320.10761105481084121No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5060.10235186792158957No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG253.887568E-545.00000436
CGACGGT302.1629821E-645.00000428
CGAATAT502.1827873E-1145.00000414
GTTACAC207.0293824E-445.033
TCGTCGG406.8012014E-945.02
TTACGCG207.0293824E-445.01
GCATATT650.041.53846427
CGTATGG556.002665E-1140.9090922
CTACGAA556.002665E-1140.90909211
GCGTAAG451.9244908E-840.01
CGTAAGG850.039.7058832
ATATTAG700.038.5714329
TACGGCT3800.038.4868437
CGTTTTT13300.038.4022561
TATGGGC1250.037.8000034
CTGAACG301.1389809E-437.50000412
ACGATTG301.1389809E-437.5000041
CCACTAA301.1389809E-437.50000421
CGCAGTA301.1389809E-437.50000437
CGATGAA3300.037.50000419