##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494373
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33126000004046	33.0	31.0	34.0	30.0	34.0
2	32.45683522360647	34.0	31.0	34.0	31.0	34.0
3	32.46445699906751	34.0	31.0	34.0	31.0	34.0
4	35.98668818887763	37.0	35.0	37.0	35.0	37.0
5	36.06348040851746	37.0	35.0	37.0	35.0	37.0
6	36.0718546522565	37.0	35.0	37.0	35.0	37.0
7	36.19636185633115	37.0	37.0	37.0	35.0	37.0
8	36.08655610237614	37.0	37.0	37.0	35.0	37.0
9	37.940292046693486	39.0	38.0	39.0	35.0	39.0
10	37.66413821143145	39.0	37.0	39.0	35.0	39.0
11	37.59721101273735	39.0	37.0	39.0	35.0	39.0
12	37.531022122971926	39.0	37.0	39.0	35.0	39.0
13	37.49095116440421	39.0	37.0	39.0	35.0	39.0
14	38.77155305811604	40.0	38.0	41.0	35.0	41.0
15	38.80052713234744	40.0	38.0	41.0	35.0	41.0
16	38.867717290386004	40.0	38.0	41.0	35.0	41.0
17	38.79401989995408	40.0	38.0	41.0	35.0	41.0
18	38.74048340018569	40.0	38.0	41.0	35.0	41.0
19	38.69044425969865	40.0	38.0	41.0	35.0	41.0
20	38.56169531912139	40.0	38.0	41.0	35.0	41.0
21	38.500041466665856	40.0	38.0	41.0	35.0	41.0
22	38.4962710342191	40.0	38.0	41.0	35.0	41.0
23	38.43944956540911	40.0	37.0	41.0	35.0	41.0
24	38.37119543340757	40.0	37.0	41.0	34.0	41.0
25	38.294399168239366	40.0	37.0	41.0	34.0	41.0
26	38.290640872377736	40.0	37.0	41.0	34.0	41.0
27	38.27653411492942	40.0	37.0	41.0	34.0	41.0
28	38.19568827585649	40.0	37.0	41.0	34.0	41.0
29	38.168919014590195	40.0	37.0	41.0	34.0	41.0
30	38.0417336707304	40.0	37.0	41.0	34.0	41.0
31	38.010283733132674	40.0	37.0	41.0	34.0	41.0
32	37.86711652942212	40.0	37.0	41.0	34.0	41.0
33	37.79078752278138	40.0	36.0	41.0	34.0	41.0
34	37.6524992262927	40.0	36.0	41.0	33.0	41.0
35	37.574260730258324	40.0	36.0	41.0	33.0	41.0
36	37.50591355110413	40.0	36.0	41.0	33.0	41.0
37	37.42864395911589	40.0	36.0	41.0	33.0	41.0
38	37.26176995911994	40.0	35.0	41.0	33.0	41.0
39	37.24987003740091	40.0	35.0	41.0	33.0	41.0
40	37.16214882285238	40.0	35.0	41.0	32.0	41.0
41	37.10425528902266	40.0	35.0	41.0	32.0	41.0
42	37.019400331328775	40.0	35.0	41.0	32.0	41.0
43	37.025126776745495	40.0	35.0	41.0	32.0	41.0
44	37.011248591650435	40.0	35.0	41.0	32.0	41.0
45	36.91272177080868	39.0	35.0	41.0	32.0	41.0
46	36.856444425565314	39.0	35.0	41.0	32.0	41.0
47	36.78910660574101	39.0	35.0	41.0	32.0	41.0
48	36.7508298390082	39.0	35.0	41.0	32.0	41.0
49	36.73097034020871	39.0	35.0	41.0	32.0	41.0
50	36.6359246965348	39.0	35.0	41.0	32.0	41.0
51	35.84193109251517	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	8.0
11	9.0
12	12.0
13	10.0
14	15.0
15	18.0
16	34.0
17	41.0
18	97.0
19	164.0
20	353.0
21	622.0
22	1005.0
23	1523.0
24	2019.0
25	2585.0
26	3541.0
27	4226.0
28	4516.0
29	4838.0
30	5465.0
31	6793.0
32	8668.0
33	12288.0
34	24442.0
35	35431.0
36	31056.0
37	47581.0
38	91529.0
39	205216.0
40	254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44088370521853	19.44139344179395	23.60403986463662	13.5136829883509
2	31.46490605271728	26.40334322465021	24.59964439805572	17.532106324576787
3	30.55466216803911	26.131685994178483	27.20820109512453	16.105450742657872
4	27.66979588286577	24.471603424944323	29.32805796433058	18.530542727859327
5	27.15014776292395	28.018520428906918	24.373701638236717	20.45763016993242
6	25.227914954902474	34.728636070335554	24.628772202365422	15.414676772396552
7	76.37451883496874	5.9420720791790815	12.817447554781511	4.86596153107067
8	76.50154842598604	7.814544888171482	10.148612484905122	5.535294200937349
9	69.78738725618106	8.314572195488022	12.134764641272886	9.76327590705803
10	37.835197310532735	27.812603034550836	18.996183043976917	15.356016610939513
11	29.505049830795777	23.126060686971172	27.506356536461336	19.862532945771715
12	25.72713315654374	20.554116021708303	31.48857239371891	22.23017842802904
13	24.482728627979277	22.22006460708817	33.02830049375674	20.268906271175812
14	20.837303008052626	27.036266139129765	29.83617632839981	22.2902545244178
15	19.74986498049044	24.64171789316973	34.36069526450676	21.247721861833067
16	21.775865591365225	23.595544255046292	31.07046703602341	23.558123117565078
17	21.340769014489062	24.60025122731217	29.696605599415825	24.36237415878294
18	22.003224286115948	23.842321486003485	30.249831604881333	23.904622622999234
19	22.58638720156643	25.065486990592124	28.048659615310704	24.29946619253074
20	23.767276934622238	25.454464543977927	29.762952264787923	21.01530625661191
21	22.884340366484416	27.312575727234297	28.804364316012403	20.998719590268884
22	21.798722826691588	23.499665232526855	28.855944802810836	25.845667137970725
23	21.682413885871597	25.896438519093884	29.133063496590633	23.288084098443885
24	22.066334528786967	24.367431069253378	29.376806581265562	24.18942782069409
25	21.181172920042155	27.701148727782467	27.361122067750465	23.756556284424917
26	20.17039765521175	25.636311044494743	29.454278449672618	24.739012850620888
27	23.164088653708838	24.93704146464309	28.03975945288274	23.859110428765327
28	19.47072352252247	26.329107778944238	30.051398438021494	24.1487702605118
29	21.194725440102918	24.458860010558826	30.26480005987382	24.081614489464435
30	22.058648024871907	25.373149423613345	28.91217764724206	23.656024904272684
31	22.744971913919247	26.077071361097794	25.85901738161267	25.31893934337029
32	22.87786752108226	26.522079482496007	28.209267091851697	22.39078590457003
33	23.365960519688574	25.039191056145864	25.104728615842692	26.490119808322866
34	20.771765448355797	25.293654791018117	28.722846919229006	25.211732841397083
35	21.985626237678837	24.37269025614263	27.732906125536793	25.90877738064174
36	21.83776217552334	27.31520532067892	26.346098998124894	24.500933505672844
37	20.474823665531897	26.63272468358911	28.295234569849082	24.59721708102991
38	21.21090755360831	27.951971487116	26.340232981979195	24.496887977296495
39	22.795743295042406	24.146747496323627	28.007192949453145	25.05031625918082
40	22.730408011764396	24.159693187127935	28.784136674130668	24.325762126977
41	19.682506933024253	25.621747142339895	26.82529183430325	27.870454090332604
42	20.107894241797187	26.009915590050426	28.534123020472396	25.34806714767999
43	21.898242824749733	23.75493807307438	28.422061884447576	25.924757217728317
44	22.28337712617801	23.79397742190613	28.8800156966501	25.04262975526576
45	21.69010038978666	23.258349464877735	28.492858631033652	26.55869151430195
46	23.43089125012895	24.8532990272527	27.67849376887492	24.03731595374343
47	20.079575543162754	24.614208300210567	31.00027711869378	24.305939037932898
48	20.454596023650158	24.785940979786517	28.98216528815287	25.77729770841045
49	21.315282185718072	23.409854502571946	30.630718101514443	24.64414521019554
50	21.81146624107708	22.377637937346904	29.998402016291344	25.812493805284674
51	21.259858446962113	22.379862977953895	27.773563685719083	28.586714889364913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	621.0
2	1060.0
3	2900.5
4	4741.0
5	3145.0
6	1549.0
7	1511.5
8	1474.0
9	1433.0
10	1392.0
11	1391.0
12	1390.0
13	1346.5
14	1303.0
15	1239.5
16	1176.0
17	1174.0
18	1172.0
19	1188.0
20	1204.0
21	1306.5
22	1409.0
23	1508.0
24	1607.0
25	2110.0
26	3293.0
27	3973.0
28	4828.5
29	5684.0
30	6400.0
31	7116.0
32	8641.0
33	10166.0
34	10629.5
35	11093.0
36	12092.0
37	13091.0
38	14274.0
39	15457.0
40	17227.5
41	18998.0
42	21270.5
43	23543.0
44	26773.0
45	30003.0
46	40928.5
47	51854.0
48	50732.0
49	49610.0
50	48214.5
51	46819.0
52	41072.5
53	35326.0
54	31438.5
55	27551.0
56	25214.0
57	22877.0
58	21919.0
59	20961.0
60	20127.0
61	19293.0
62	18087.0
63	16881.0
64	15015.5
65	13150.0
66	11304.0
67	9458.0
68	7949.5
69	6441.0
70	5347.5
71	4254.0
72	3756.5
73	3259.0
74	2700.5
75	1782.5
76	1423.0
77	1176.5
78	930.0
79	667.5
80	405.0
81	287.0
82	169.0
83	142.0
84	115.0
85	69.5
86	24.0
87	26.0
88	28.0
89	17.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	494373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.03463680605398
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17057305733564	23.119580959765322
2	9.12772100208274	5.8480645433742335
3	3.403399928228622	3.2708004181966226
4	1.9333354281390869	2.477347930588501
5	1.3895810605162182	2.225736229310418
6	1.1383533554426069	2.1880041779134065
7	0.9756685720509096	2.1878631844112535
8	0.8466394119854113	2.1697428854915
9	0.8106594945398785	2.3372264232867876
>10	8.084418193466561	45.71655375148125
>50	0.06520635507866802	1.3756068236818506
>100	0.04558114141421454	2.6875337796597205
>500	0.00506457126824606	1.0949286806808656
>1k	0.0037984284511845454	3.3010102121582605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	3465	0.7008877912021895	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCG	3392	0.6861216126285213	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGC	3367	0.6810647021580871	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2848	0.5760832407918718	No Hit
GCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2194	0.44379446288531127	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGCT	1011	0.2045014594243618	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	975	0.1972195083469364	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCC	749	0.15150503769421064	No Hit
TCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	747	0.1511004848565759	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	674	0.13633430628290785	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCATGCACT	617	0.12480455041031771	No Hit
GAACTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	599	0.12116357487160505	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCATGCACTCGTA	532	0.10761105481084121	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	506	0.10235186792158957	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.022764188173707E-4	0.0	0.0	0.20733332928780496	0.0
2	2.022764188173707E-4	0.0	0.0	1.109283880794461	0.0
3	2.022764188173707E-4	0.0	0.0	1.4834952556065966	0.0
4	2.022764188173707E-4	0.0	0.0	2.041373618704905	0.0
5	2.022764188173707E-4	0.0	0.0	3.9269943949204347	0.0
6	2.022764188173707E-4	0.0	0.0	4.770284784970053	0.0
7	2.022764188173707E-4	0.0	0.0	5.648771271893894	0.0
8	2.022764188173707E-4	0.0	0.0	6.91846035281053	0.0
9	2.022764188173707E-4	0.0	0.0	7.441749448291068	0.0
10	2.022764188173707E-4	0.0	0.0	9.087268115370378	0.0
11	2.022764188173707E-4	0.0	0.0	10.408335406666627	0.0
12	2.022764188173707E-4	0.0	0.0	12.113323340878244	0.0
13	2.022764188173707E-4	0.0	0.0	12.571883982337223	0.0
14	2.022764188173707E-4	0.0	0.0	12.755753247042213	0.0
15	2.022764188173707E-4	0.0	0.0	13.16758803575438	0.0
16	2.022764188173707E-4	0.0	0.0	13.821749974209757	0.0
17	2.022764188173707E-4	0.0	0.0	14.57846605700554	0.0
18	2.022764188173707E-4	0.0	0.0	15.406181162806222	0.0
19	2.022764188173707E-4	0.0	0.0	16.017460500472314	0.0
20	2.022764188173707E-4	0.0	0.0	16.507576263266806	0.0
21	2.022764188173707E-4	0.0	0.0	17.069095601903825	0.0
22	2.022764188173707E-4	0.0	0.0	17.683813638689816	0.0
23	2.022764188173707E-4	0.0	0.0	18.24735574151501	0.0
24	2.022764188173707E-4	0.0	0.0	18.70510727729872	0.0
25	2.022764188173707E-4	0.0	0.0	19.14748580525231	0.0
26	2.022764188173707E-4	0.0	0.0	19.52918140756069	0.0
27	2.022764188173707E-4	0.0	0.0	19.938993432084683	0.0
28	2.022764188173707E-4	0.0	0.0	20.33929846492426	0.0
29	2.022764188173707E-4	0.0	0.0	20.751537806474058	0.0
30	2.022764188173707E-4	0.0	0.0	21.224257797250253	0.0
31	2.022764188173707E-4	0.0	0.0	21.63083339907317	0.0
32	2.022764188173707E-4	0.0	0.0	22.043477293460604	0.0
33	2.022764188173707E-4	0.0	0.0	22.44317549704373	0.0
34	2.022764188173707E-4	0.0	0.0	22.8317484975919	0.0
35	2.022764188173707E-4	0.0	0.0	23.25632670068956	0.0
36	2.022764188173707E-4	0.0	0.0	23.655418075016232	0.0
37	2.022764188173707E-4	0.0	0.0	24.07271432703647	0.0
38	2.022764188173707E-4	0.0	0.0	24.46958066075615	0.0
39	2.022764188173707E-4	0.0	0.0	24.897799839392523	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	25	3.887568E-5	45.000004	36
CGACGGT	30	2.1629821E-6	45.000004	28
CGAATAT	50	2.1827873E-11	45.000004	14
GTTACAC	20	7.0293824E-4	45.0	33
TCGTCGG	40	6.8012014E-9	45.0	2
TTACGCG	20	7.0293824E-4	45.0	1
GCATATT	65	0.0	41.538464	27
CGTATGG	55	6.002665E-11	40.909092	2
CTACGAA	55	6.002665E-11	40.909092	11
GCGTAAG	45	1.9244908E-8	40.0	1
CGTAAGG	85	0.0	39.705883	2
ATATTAG	70	0.0	38.57143	29
TACGGCT	380	0.0	38.486843	7
CGTTTTT	1330	0.0	38.402256	1
TATGGGC	125	0.0	37.800003	4
CTGAACG	30	1.1389809E-4	37.500004	12
ACGATTG	30	1.1389809E-4	37.500004	1
CCACTAA	30	1.1389809E-4	37.500004	21
CGCAGTA	30	1.1389809E-4	37.500004	37
CGATGAA	330	0.0	37.500004	19
>>END_MODULE