##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	534903
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32715090399568	33.0	31.0	34.0	30.0	34.0
2	32.47578906829836	34.0	31.0	34.0	31.0	34.0
3	32.432528888415284	34.0	31.0	34.0	30.0	34.0
4	35.98839976593887	37.0	35.0	37.0	35.0	37.0
5	36.060248306702334	37.0	35.0	37.0	35.0	37.0
6	36.06932658818515	37.0	35.0	37.0	35.0	37.0
7	36.19041770190109	37.0	37.0	37.0	35.0	37.0
8	36.07872829279327	37.0	37.0	37.0	35.0	37.0
9	37.940194764284364	39.0	38.0	39.0	35.0	39.0
10	37.664574698590215	39.0	37.0	39.0	35.0	39.0
11	37.629663696034605	39.0	37.0	39.0	35.0	39.0
12	37.604137572606625	39.0	37.0	39.0	35.0	39.0
13	37.59660723533052	39.0	37.0	39.0	35.0	39.0
14	38.93950491958355	40.0	38.0	41.0	35.0	41.0
15	38.98822216364462	40.0	38.0	41.0	35.0	41.0
16	39.012869623090545	40.0	38.0	41.0	35.0	41.0
17	38.94585560372629	40.0	38.0	41.0	35.0	41.0
18	38.76022568577854	40.0	38.0	41.0	35.0	41.0
19	38.56360125106795	40.0	37.0	41.0	35.0	41.0
20	38.33706859000604	40.0	36.0	41.0	35.0	41.0
21	38.25317674419474	40.0	36.0	41.0	35.0	41.0
22	38.24903206749635	40.0	36.0	41.0	35.0	41.0
23	38.20309476671471	40.0	36.0	41.0	35.0	41.0
24	38.113452345565456	40.0	36.0	41.0	34.0	41.0
25	38.01258919841541	40.0	35.0	41.0	34.0	41.0
26	38.022654574754675	40.0	35.0	41.0	34.0	41.0
27	37.99773790762063	40.0	35.0	41.0	34.0	41.0
28	37.89556798148449	40.0	35.0	41.0	34.0	41.0
29	37.811219978201656	40.0	35.0	41.0	34.0	41.0
30	37.61010314019551	40.0	35.0	41.0	33.0	41.0
31	37.46275305990058	40.0	35.0	41.0	33.0	41.0
32	37.15478320368366	40.0	35.0	41.0	33.0	41.0
33	36.87315644144826	40.0	35.0	41.0	31.0	41.0
34	36.5796864104333	40.0	35.0	41.0	30.0	41.0
35	36.400726860757935	40.0	35.0	41.0	30.0	41.0
36	36.26451150956342	40.0	35.0	41.0	29.0	41.0
37	36.161636782743784	40.0	35.0	41.0	29.0	41.0
38	36.02929316156387	39.0	35.0	41.0	27.0	41.0
39	36.00291080812783	39.0	35.0	41.0	27.0	41.0
40	35.93833087494368	39.0	35.0	41.0	27.0	41.0
41	35.831027307754866	39.0	35.0	41.0	26.0	41.0
42	35.72799367361933	39.0	35.0	41.0	26.0	41.0
43	35.709764200238176	39.0	35.0	41.0	26.0	41.0
44	35.67155914249874	39.0	35.0	41.0	26.0	41.0
45	35.5788283109274	39.0	35.0	41.0	25.0	41.0
46	35.51462227731009	39.0	35.0	41.0	25.0	41.0
47	35.44128748576845	39.0	35.0	41.0	25.0	41.0
48	35.40654660751576	39.0	35.0	41.0	25.0	41.0
49	35.3569936979228	39.0	35.0	41.0	25.0	41.0
50	35.272008195878506	38.0	35.0	41.0	25.0	41.0
51	34.528671553534004	37.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	10.0
10	13.0
11	13.0
12	12.0
13	13.0
14	23.0
15	33.0
16	40.0
17	72.0
18	146.0
19	272.0
20	517.0
21	869.0
22	1315.0
23	1885.0
24	3132.0
25	5358.0
26	8310.0
27	9887.0
28	9281.0
29	8326.0
30	7894.0
31	8578.0
32	10234.0
33	13451.0
34	24137.0
35	35649.0
36	37833.0
37	51230.0
38	93766.0
39	202292.0
40	308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.31236130662943	18.37192911612012	21.38369012699499	20.932019450255467
2	37.250305195521435	24.33712280544323	22.153549335113095	16.25902266392224
3	28.161741474622502	23.447054886586912	32.5681478698007	15.82305576898989
4	25.983028698661254	22.77534431476361	34.02766482895029	17.21396215762484
5	24.835157028470583	26.110715400736208	30.39523053712542	18.658897033667788
6	23.70990628207357	32.168262283068145	29.90130920933328	14.220522225525
7	70.79563958325154	5.859940961258396	18.416423164573764	4.927996290916297
8	71.47613679489552	6.868348093018735	16.418677778961793	5.236837333123949
9	65.2335096269791	7.62867286218249	18.65983178258488	8.477985728253534
10	35.32341377782514	23.47210615756502	25.819260688386493	15.385219376223352
11	26.453020454175803	22.57474719715537	31.68555794228112	19.2866744063877
12	23.20476796727631	19.756853111685672	35.9326831219866	21.105695799051418
13	22.53511384307061	21.5850350437369	37.79825501072157	18.081596102470915
14	19.29228289989026	24.469109352536815	35.47372140369375	20.764886343879173
15	18.743772235339865	23.514543758401054	37.89229075178117	19.849393254477913
16	20.162721091487615	23.152982877269338	35.32977007046137	21.354525960781675
17	20.50708259254482	24.00584778922534	33.8044467875484	21.68262283068145
18	20.47249688261236	23.186820788068115	34.62253903978853	21.718143289531
19	21.080644528073314	25.283462609108565	31.986173194018352	21.649719668799765
20	21.89593253356216	24.593617908293652	33.01047105736928	20.49997850077491
21	21.548205936403424	26.1656786370613	32.68947827923942	19.596637147295866
22	20.72300959239339	23.05763848772581	32.74182421859664	23.47752770128416
23	20.781524874603434	24.60932169010082	32.92241770937908	21.686735725916662
24	20.95015357924708	23.735331452618514	33.56832921109061	21.7461857570438
25	20.811062940383586	26.19502975305803	31.747251370809288	21.246655935749096
26	20.290033893995734	24.39376858981909	32.50682834083937	22.80936917534581
27	22.2406679341862	23.74299639373868	32.01832855676637	21.998007115308756
28	20.411551253217873	24.19896691549683	33.13460571355928	22.254876117726017
29	21.45248764729306	22.537357240471636	32.04506237579523	23.965092736440067
30	22.03539707199249	23.534734335010274	31.857177843459468	22.572690749537767
31	22.390975560054812	23.181773143915812	31.404572417802857	23.022678878226518
32	21.852747133592445	24.223457337124675	31.629659957038943	22.29413557224394
33	21.590269637672623	23.53286483717609	30.288669160576774	24.58819636457451
34	19.99577493489474	23.74804403789098	32.07104839569043	24.185132631523846
35	21.319192451715544	24.066980368403243	31.00076088561851	23.613066294262698
36	20.952396976648103	25.835899219110754	29.44720818540932	23.764495618831827
37	20.61663516562816	25.56239168596923	31.93794014989634	21.883032998506273
38	19.66132177235873	26.00976251769012	30.742209335150484	23.586706374800663
39	20.779281477202407	24.487617381095266	31.327362157250942	23.405738984451386
40	21.417901937360607	24.350396240065955	31.744447124058006	22.487254698515432
41	19.622623167191062	25.338986694783916	30.46047601153854	24.577914126486483
42	20.345744929454497	25.707464717902123	30.862978895238953	23.083811457404426
43	21.601486624677747	23.693828600699565	30.959631933266408	23.745052841356284
44	21.77291957607267	24.082497200426996	30.521421640933028	23.623161582567306
45	21.19599254444264	23.393400298745753	30.486462031433735	24.924145125377873
46	22.21972955844331	24.347031143964422	30.135370338173463	23.29786895941881
47	20.15468225080061	24.316745279050593	32.96990295436743	22.558669515781364
48	20.19842850012058	23.841145030033484	31.542728307749258	24.41769816209668
49	20.477357576981248	23.360497136864065	32.62778485071125	23.534360435443435
50	19.822472485665628	22.934438580452905	33.30622561473763	23.936863319143846
51	19.617762472822175	22.801143384875388	31.356526323464255	26.224567818838178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	189.0
1	524.5
2	860.0
3	9393.0
4	17926.0
5	11299.5
6	4673.0
7	4576.5
8	4480.0
9	4399.0
10	4318.0
11	4321.5
12	4325.0
13	4098.0
14	3871.0
15	3683.0
16	3495.0
17	3231.5
18	2968.0
19	2823.5
20	2679.0
21	2593.0
22	2507.0
23	2605.5
24	2704.0
25	3102.5
26	4147.5
27	4794.0
28	5339.0
29	5884.0
30	6798.5
31	7713.0
32	8742.0
33	9771.0
34	11317.0
35	12863.0
36	13743.0
37	14623.0
38	15217.5
39	15812.0
40	17754.0
41	19696.0
42	21619.5
43	23543.0
44	26758.5
45	29974.0
46	40159.0
47	50344.0
48	49331.0
49	48318.0
50	46940.5
51	45563.0
52	39930.5
53	34298.0
54	31137.5
55	27977.0
56	25546.5
57	23116.0
58	22107.5
59	21099.0
60	19727.0
61	18355.0
62	17277.5
63	16200.0
64	14832.5
65	13465.0
66	11444.5
67	9424.0
68	8643.5
69	7863.0
70	6560.0
71	5257.0
72	4796.0
73	4335.0
74	3574.0
75	2152.0
76	1491.0
77	1211.0
78	931.0
79	711.0
80	491.0
81	364.5
82	238.0
83	163.0
84	88.0
85	62.0
86	36.0
87	30.5
88	25.0
89	14.5
90	4.0
91	2.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	534903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.57163064045829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70225476811777	28.04244517644336
2	8.438414759262203	6.509668345705068
3	3.7404483622130815	4.328255779709703
4	2.585245436429748	3.9886852835559448
5	1.9528326307768833	3.7661969468480203
6	1.6144716256751523	3.7363681935025324
7	1.3651409482387664	3.685899869934148
8	1.1710204691720933	3.6134535207457725
9	1.0319246872334462	3.5822716096265035
>10	5.311664718335887	29.457432648718534
>50	0.05350547977514298	1.4197023813486789
>100	0.025779912982361597	2.18930977769992
>500	0.0038913076199791085	1.1808651761799038
>1k	0.0029184807149843315	2.112949414384823
>5k	0.0	0.0
>10k+	4.8641345249738856E-4	2.3864958755971366
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12720	2.3780012450855574	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	3395	0.6346945147064047	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCG	2346	0.4385841919002137	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGC	1845	0.34492235040745706	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTT	1354	0.2531300067488872	No Hit
GCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	1294	0.24191301974376664	TruSeq Adapter, Index 23 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTC	1028	0.19218437735439883	No Hit
GAACTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	949	0.17741534446432344	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	878	0.16414190984159743	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCC	854	0.15965511503954924	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCT	820	0.15329882240331424	No Hit
CCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGC	812	0.15180322413596484	TruSeq Adapter, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTGCT	695	0.12993009947597975	TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCTG	683	0.12768670207495564	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTTCTACTCGTATGCCGTCTTCT	603	0.11273071940146157	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13142569774332916	0.0
2	0.0	0.0	0.0	0.647780999545712	0.0
3	0.0	0.0	0.0	1.0338323023052778	0.0
4	0.0	0.0	0.0	1.6417929979828119	0.0
5	0.0	0.0	0.0	3.3308842911705487	0.0
6	0.0	0.0	0.0	4.742729055548389	0.0
7	0.0	0.0	0.0	5.811334017569541	0.0
8	0.0	0.0	0.0	7.231591522201222	0.0
9	0.0	0.0	0.0	7.975277760640714	0.0
10	0.0	0.0	0.0	9.432364372605875	0.0
11	0.0	0.0	0.0	10.528264003006152	0.0
12	0.0	0.0	0.0	11.69931744634074	0.0
13	0.0	0.0	0.0	12.127432450369506	0.0
14	0.0	0.0	0.0	12.364671725527806	0.0
15	0.0	0.0	0.0	12.684729754740578	0.0
16	0.0	0.0	0.0	13.105927616782855	0.0
17	0.0	0.0	0.0	13.599101145441322	0.0
18	0.0	0.0	0.0	14.163502541582305	0.0
19	0.0	0.0	0.0	14.54263670235538	0.0
20	0.0	0.0	0.0	14.896345692583514	0.0
21	0.0	0.0	0.0	15.289874986679829	0.0
22	0.0	0.0	0.0	15.712568446989454	0.0
23	0.0	0.0	0.0	16.135448857082498	0.0
24	0.0	0.0	0.0	16.497757537347894	0.0
25	0.0	0.0	0.0	16.806598579555544	0.0
26	0.0	0.0	0.0	17.100109739522868	0.0
27	0.0	0.0	0.0	17.412315877832057	0.0
28	0.0	0.0	0.0	17.720596070689453	0.0
29	0.0	0.0	0.0	18.060844676511444	0.0
30	0.0	0.0	0.0	18.472134200032528	0.0
31	1.869497834186759E-4	0.0	0.0	18.803035316683587	0.0
32	1.869497834186759E-4	0.0	0.0	19.150201064492066	0.0
33	1.869497834186759E-4	0.0	0.0	19.48315862876073	0.0
34	1.869497834186759E-4	0.0	0.0	19.81592924324597	0.0
35	1.869497834186759E-4	0.0	0.0	20.160290744303172	0.0
36	1.869497834186759E-4	0.0	0.0	20.48109657264962	0.0
37	1.869497834186759E-4	0.0	0.0	20.808632593199142	0.0
38	1.869497834186759E-4	0.0	0.0	21.143085755735154	0.0
39	1.869497834186759E-4	0.0	0.0	21.478473667188258	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATG	20	7.029902E-4	45.0	1
TACGAAT	150	0.0	45.0	12
CGAATAT	150	0.0	45.0	14
CGATGTA	20	7.029902E-4	45.0	10
GCGTATG	25	3.888E-5	45.0	1
CGTTTTT	5210	0.0	43.833973	1
CTACGAA	155	0.0	43.548386	11
GCTACGA	155	0.0	43.548386	10
CCTATGC	155	0.0	42.09677	35
TATTAGT	60	3.6379788E-12	41.249996	30
CATACGA	160	0.0	40.78125	18
CACAACG	50	1.0786607E-9	40.5	12
ACATACG	165	0.0	39.545452	17
TTGCGAG	105	0.0	38.57143	1
ACGCTAG	35	6.2420277E-6	38.571426	1
CGGTCTA	35	6.2420277E-6	38.571426	31
CGTTTCT	140	0.0	38.571426	1
AGCTACG	170	0.0	38.38235	9
GTTTTTT	6175	0.0	37.676113	2
AATGGGC	150	0.0	37.5	4
>>END_MODULE