FastQCFastQC Report
Sat 18 Jun 2016
SRR3551491_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551491_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences627853
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57960.9231460230340542No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC54030.8605517533562793No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG52300.8329975328619916No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC46760.7447603180999374No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC21150.33686229101397935No Hit
GAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT11120.17711152132744448No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT10510.16739587132656847No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC8850.14095656148811905No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT8340.13283364099558337Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC8180.13028527378223884No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC7950.1266219959130561No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGT7710.12279944509303929No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7110.11324306804299733No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7100.11308379509216329No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTA6460.10289032623878519No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT207.0308376E-445.0000045
ATTCGCG207.0308376E-445.0000041
ACGATTG253.888776E-545.01
TCGACAG351.2107557E-745.01
CGTTTAT502.1827873E-1145.039
CGTTCGG600.044.99999645
CGCATCG556.002665E-1140.90909221
CGTTTTT23500.040.8829771
AAACTAT1050.040.7142876
TACGGCT6650.040.6015057
CGGTCTA403.4548793E-739.37500431
GATGAAT6100.039.09836220
GTCGAAT700.038.57142643
TAGTAAG659.094947E-1238.076921
GCATATT659.094947E-1238.0769227
TCGTTTA601.546141E-1037.49999638
ATTATCC601.546141E-1037.49999645
CGATCAC301.1393322E-437.49999634
CGATGAA5800.037.2413819
CCGATGA5500.037.22727218