##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551491_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	627853
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.311087149380505	33.0	31.0	34.0	30.0	34.0
2	32.434620842776894	34.0	31.0	34.0	31.0	34.0
3	32.4477608612207	34.0	31.0	34.0	30.0	34.0
4	35.97940282199814	37.0	35.0	37.0	35.0	37.0
5	36.03229736897013	37.0	35.0	37.0	35.0	37.0
6	36.037094670249246	37.0	35.0	37.0	35.0	37.0
7	36.136442766061485	37.0	36.0	37.0	35.0	37.0
8	35.99330097968792	37.0	36.0	37.0	35.0	37.0
9	37.84907135906016	39.0	38.0	39.0	35.0	39.0
10	37.626019147794146	39.0	37.0	39.0	35.0	39.0
11	37.567655167690525	39.0	37.0	39.0	35.0	39.0
12	37.47813739840377	39.0	37.0	39.0	35.0	39.0
13	37.42190767584132	39.0	37.0	39.0	35.0	39.0
14	38.743396941640796	40.0	38.0	41.0	35.0	41.0
15	38.75508279804349	40.0	38.0	41.0	35.0	41.0
16	38.83037430736176	40.0	38.0	41.0	35.0	41.0
17	38.73378004086944	40.0	38.0	41.0	35.0	41.0
18	38.65618863014113	40.0	38.0	41.0	35.0	41.0
19	38.58356335001983	40.0	37.0	41.0	35.0	41.0
20	38.418217321570495	40.0	37.0	41.0	34.0	41.0
21	38.36651413627075	40.0	37.0	41.0	34.0	41.0
22	38.31547034098746	40.0	37.0	41.0	34.0	41.0
23	38.229181034414104	40.0	37.0	41.0	34.0	41.0
24	38.13966963604538	40.0	37.0	41.0	34.0	41.0
25	38.06869283096521	40.0	36.0	41.0	34.0	41.0
26	38.053471115054	40.0	36.0	41.0	34.0	41.0
27	37.995878016032414	40.0	36.0	41.0	34.0	41.0
28	37.89007140206386	40.0	36.0	41.0	34.0	41.0
29	37.844403068871216	40.0	36.0	41.0	33.0	41.0
30	37.70821195407205	40.0	36.0	41.0	33.0	41.0
31	37.7104369971952	40.0	36.0	41.0	33.0	41.0
32	37.49336867069203	40.0	35.0	41.0	33.0	41.0
33	37.3522576144416	40.0	35.0	41.0	33.0	41.0
34	37.18120483616388	40.0	35.0	41.0	32.0	41.0
35	37.072200021342574	40.0	35.0	41.0	32.0	41.0
36	36.9535560075368	40.0	35.0	41.0	32.0	41.0
37	36.853999264158965	39.0	35.0	41.0	31.0	41.0
38	36.69953794916963	39.0	35.0	41.0	31.0	41.0
39	36.609641110259886	39.0	35.0	41.0	31.0	41.0
40	36.4907295178967	39.0	35.0	41.0	31.0	41.0
41	36.41467349841444	39.0	35.0	41.0	30.0	41.0
42	36.30777586473267	39.0	35.0	41.0	30.0	41.0
43	36.24989766712909	39.0	35.0	41.0	31.0	41.0
44	36.226382608667954	39.0	35.0	41.0	31.0	41.0
45	36.15332410612038	39.0	35.0	41.0	30.0	41.0
46	36.091008564106566	38.0	35.0	41.0	30.0	41.0
47	35.98899264636786	38.0	35.0	41.0	30.0	41.0
48	35.94424809629005	38.0	35.0	41.0	30.0	41.0
49	35.89264206749032	38.0	35.0	40.0	30.0	41.0
50	35.781993555816406	38.0	35.0	40.0	30.0	41.0
51	34.95543065016811	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	15.0
10	23.0
11	20.0
12	26.0
13	22.0
14	32.0
15	27.0
16	42.0
17	106.0
18	170.0
19	314.0
20	578.0
21	1048.0
22	1765.0
23	2498.0
24	3266.0
25	4295.0
26	5592.0
27	6717.0
28	7002.0
29	7290.0
30	8125.0
31	9608.0
32	12424.0
33	17067.0
34	32624.0
35	47608.0
36	47061.0
37	69243.0
38	118389.0
39	224553.0
40	295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.05179715634074	19.042674001716964	22.36335575365571	15.54217308828659
2	30.830465092943733	28.392633307478025	23.71829074640083	17.058610853177417
3	29.896647782203793	26.820290736844456	26.7704383032334	16.512623177718353
4	27.776087714799484	22.96365550534918	30.51335264783317	18.746904132018162
5	27.598020555767032	27.52316226887504	25.160985135055498	19.717832040302426
6	25.25591181375258	35.90139730159766	24.20248051693629	14.640210367713461
7	74.33762361571897	6.560612117804646	14.107123801271953	4.9946404652044345
8	74.61969601164604	9.027909399174648	11.003212535418323	5.349182053760992
9	68.69776842668587	7.745284326108182	12.682108710159861	10.874838537046092
10	41.96061817017678	22.313344047093825	19.512688479628192	16.213349303101204
11	31.494155479069146	22.946294753708273	24.843394871092435	20.716154896130146
12	25.993823394966658	20.176219592802774	29.95621586581572	23.873741146414844
13	25.102531962099405	21.1156114568219	32.77248018246309	21.0093763986156
14	21.6163656142441	25.774663814619025	29.288702928870293	23.320267642266582
15	20.763140416626186	22.22813301839762	33.83546785632943	23.173258708646767
16	22.56515458236243	22.674734372536246	29.78579380842331	24.97431723667801
17	22.693847126636328	23.468869305394733	28.98273959031812	24.85454397765082
18	22.566906584821606	22.535689086458135	29.405609274782474	25.491795053937782
19	24.34375562432608	23.503590808676552	27.19665271966527	24.9560008473321
20	25.84872573675685	23.469824943099738	28.435318458301545	22.246130861841866
21	24.92446480306696	25.566016249026447	27.708874529547522	21.800644418359074
22	23.50390935457822	21.848107757707616	28.042710634495656	26.60527225321851
23	24.254562771859018	23.986187849703672	27.289190304099847	24.470059074337463
24	23.561725435730978	23.85542475706893	27.592127456586173	24.990722350613918
25	23.570326175076012	26.98832369997436	26.078875150712026	23.3624749742376
26	22.65355107007532	23.87979351854654	27.853175823003156	25.613479588374986
27	25.905586180204605	23.410575405389476	26.323199857291435	24.360638557114484
28	22.038598206905117	24.812177372728968	28.537890238638663	24.61133418172725
29	23.98188748003115	22.65546234548533	27.875314763169083	25.487335411314433
30	22.857261174192047	25.047901339963335	26.59523805731596	25.49959942852865
31	24.32846542104601	23.557106520156786	24.41383572269305	27.70059233610415
32	25.098868684230226	24.532016252211903	24.731107440754442	25.63800762280343
33	24.238954022677284	24.560048291558694	23.400222663585264	27.800775022178758
34	22.98372389715427	24.097041823484158	27.13103226392165	25.78820201543992
35	25.65329782608349	22.333093892997248	25.36278396376222	26.65082431715704
36	25.068925369473426	25.28028057523019	25.241736521128356	24.40905753416803
37	24.031262094789703	23.73055476361505	26.635215567975308	25.60296757361994
38	24.09831600709083	25.78867983429242	24.320820319405975	25.792183839210768
39	24.916660428476092	23.668756858691445	26.784295049955958	24.6302876628765
40	24.770129313708782	23.606003316062836	28.11135727630512	23.512510093923257
41	23.429847432440397	23.644388097213838	27.10570786473904	25.820056605606727
42	23.870555687398166	23.236330797177047	27.79806738201458	25.09504613341021
43	25.45261390803261	21.62225871342496	27.159860668022613	25.765266710519818
44	24.11965858250259	22.474369000387036	26.865364981930483	26.54060743517989
45	23.481133322608954	21.824694633935014	27.125457710642458	27.56871433281357
46	25.518234363776234	23.768939544766052	26.61673990567856	24.096086185779157
47	22.934827101248224	23.577812003765214	29.845202619084404	23.642158275902162
48	23.403726668503616	23.716538743941655	26.816468186024434	26.063266401530292
49	23.291120692263952	22.66071835286285	29.064924432948473	24.983236521924717
50	23.19141582504185	22.16936129953986	28.42178025748065	26.217442617937635
51	22.632049221712723	22.80406400861348	26.53965179747489	28.024234972198908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	293.0
1	819.0
2	1345.0
3	5080.5
4	8816.0
5	5660.0
6	2504.0
7	2374.0
8	2244.0
9	2201.0
10	2158.0
11	2065.0
12	1972.0
13	1968.0
14	1964.0
15	1880.5
16	1797.0
17	1781.5
18	1766.0
19	1718.5
20	1671.0
21	1874.0
22	2077.0
23	2114.0
24	2151.0
25	2841.5
26	4003.5
27	4475.0
28	5483.5
29	6492.0
30	7203.0
31	7914.0
32	8457.0
33	9000.0
34	9825.0
35	10650.0
36	11184.0
37	11718.0
38	12631.5
39	13545.0
40	15678.5
41	17812.0
42	20090.5
43	22369.0
44	25830.5
45	29292.0
46	31468.0
47	33644.0
48	48729.0
49	63814.0
50	57452.5
51	51091.0
52	50538.5
53	49986.0
54	45170.0
55	40354.0
56	38099.0
57	35844.0
58	34621.0
59	33398.0
60	32309.5
61	31221.0
62	29321.5
63	27422.0
64	25818.5
65	24215.0
66	21181.5
67	18148.0
68	16197.5
69	14247.0
70	12908.5
71	11570.0
72	10352.0
73	9134.0
74	7769.5
75	5349.0
76	4293.0
77	3504.0
78	2715.0
79	2059.5
80	1404.0
81	1061.5
82	719.0
83	569.5
84	420.0
85	287.5
86	155.0
87	102.5
88	50.0
89	37.0
90	24.0
91	17.0
92	10.0
93	11.0
94	12.0
95	6.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	627853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.790507971057156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.59566317217292	21.328711744237406
2	7.934364346401908	4.727374886135155
3	3.125856001924663	2.7936251442514055
4	2.0070989405556414	2.3916998794929283
5	1.4851434219444106	2.2121588474799028
6	1.294381632293855	2.3136171800664034
7	1.0869153911356897	2.266583313644473
8	1.0269344180256013	2.447431837275569
9	0.9636383648510716	2.5836548750381096
>10	9.345016850231167	47.35777242121196
>50	0.07959419979495783	1.6277635606327974
>100	0.04517508637010081	2.5401763045106778
>500	0.0064535837671572584	1.3427447245729416
>1k	0.002151194589052419	1.434546744281842
>5k	0.0016133959417893146	2.6321385371684594
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5796	0.9231460230340542	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	5403	0.8605517533562793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	5230	0.8329975328619916	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC	4676	0.7447603180999374	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2115	0.33686229101397935	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	1112	0.17711152132744448	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	1051	0.16739587132656847	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	885	0.14095656148811905	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	834	0.13283364099558337	Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	818	0.13028527378223884	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC	795	0.1266219959130561	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGT	771	0.12279944509303929	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	711	0.11324306804299733	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	710	0.11308379509216329	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTA	646	0.10289032623878519	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13649691886476611	0.0
2	0.0	0.0	0.0	0.737911581214074	0.0
3	0.0	0.0	0.0	1.039733823044566	0.0
4	0.0	0.0	0.0	1.5215344993175155	0.0
5	0.0	0.0	0.0	3.2053681355349104	0.0
6	0.0	0.0	0.0	4.025623832330179	0.0
7	0.0	0.0	0.0	4.816891852073654	0.0
8	0.0	0.0	0.0	5.925909408731025	0.0
9	0.0	0.0	0.0	6.432397392383249	0.0
10	0.0	0.0	0.0	8.074342242531293	0.0
11	0.0	0.0	0.0	8.971208228677732	0.0
12	0.0	0.0	0.0	10.455950676352586	0.0
13	0.0	0.0	0.0	10.79934315835076	0.0
14	0.0	0.0	0.0	10.965146300168989	0.0
15	0.0	0.0	0.0	11.291496576427923	0.0
16	0.0	0.0	0.0	11.7341161067957	0.0
17	0.0	0.0	0.0	12.231047713397881	0.0
18	0.0	0.0	0.0	12.815579442958782	0.0
19	0.0	0.0	0.0	13.26456989135992	0.0
20	0.0	0.0	0.0	13.608280919259762	0.0
21	0.0	0.0	0.0	13.978271984047222	0.0
22	0.0	0.0	0.0	14.389514743100694	0.0
23	0.0	0.0	0.0	14.765876725921514	0.0
24	0.0	0.0	0.0	15.114684488248045	0.0
25	0.0	0.0	0.0	15.422240556308562	0.0
26	0.0	0.0	0.0	15.70176458502229	0.0
27	0.0	0.0	0.0	16.006613012918628	0.0
28	0.0	0.0	0.0	16.3036570662241	0.0
29	0.0	0.0	0.0	16.60022330067707	0.0
30	0.0	0.0	0.0	16.938996867101057	0.0
31	1.592729508340328E-4	0.0	0.0	17.228714364668164	0.0
32	3.185459016680656E-4	0.0	0.0	17.537385343384518	0.0
33	3.185459016680656E-4	0.0	0.0	17.83442939668999	0.0
34	3.185459016680656E-4	0.0	0.0	18.123350529502925	0.0
35	3.185459016680656E-4	0.0	0.0	18.437436788547636	0.0
36	3.185459016680656E-4	0.0	0.0	18.72747283201641	0.0
37	3.185459016680656E-4	0.0	0.0	19.01957942384603	0.0
38	3.185459016680656E-4	0.0	0.0	19.312960199282315	0.0
39	3.185459016680656E-4	0.0	0.0	19.609844979636954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.0308376E-4	45.000004	5
ATTCGCG	20	7.0308376E-4	45.000004	1
ACGATTG	25	3.888776E-5	45.0	1
TCGACAG	35	1.2107557E-7	45.0	1
CGTTTAT	50	2.1827873E-11	45.0	39
CGTTCGG	60	0.0	44.999996	45
CGCATCG	55	6.002665E-11	40.909092	21
CGTTTTT	2350	0.0	40.882977	1
AAACTAT	105	0.0	40.714287	6
TACGGCT	665	0.0	40.601505	7
CGGTCTA	40	3.4548793E-7	39.375004	31
GATGAAT	610	0.0	39.098362	20
GTCGAAT	70	0.0	38.571426	43
TAGTAAG	65	9.094947E-12	38.07692	1
GCATATT	65	9.094947E-12	38.07692	27
TCGTTTA	60	1.546141E-10	37.499996	38
ATTATCC	60	1.546141E-10	37.499996	45
CGATCAC	30	1.1393322E-4	37.499996	34
CGATGAA	580	0.0	37.24138	19
CCGATGA	550	0.0	37.227272	18
>>END_MODULE