##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551490_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 327981 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.347157304843876 33.0 31.0 34.0 30.0 34.0 2 32.47185965040658 34.0 31.0 34.0 31.0 34.0 3 32.483149938563514 34.0 31.0 34.0 31.0 34.0 4 36.00175315033493 37.0 35.0 37.0 35.0 37.0 5 36.06720816144838 37.0 35.0 37.0 35.0 37.0 6 36.08055039773645 37.0 35.0 37.0 35.0 37.0 7 36.203795341803335 37.0 37.0 37.0 35.0 37.0 8 36.07862650580369 37.0 37.0 37.0 35.0 37.0 9 37.94322232080517 39.0 38.0 39.0 35.0 39.0 10 37.7027907104375 39.0 37.0 39.0 35.0 39.0 11 37.63668017354664 39.0 37.0 39.0 35.0 39.0 12 37.53884218902924 39.0 37.0 39.0 35.0 39.0 13 37.462700583265494 39.0 37.0 39.0 35.0 39.0 14 38.762208786484585 40.0 38.0 41.0 35.0 41.0 15 38.789865876376986 40.0 38.0 41.0 35.0 41.0 16 38.87827343657102 40.0 38.0 41.0 35.0 41.0 17 38.783828941310624 40.0 38.0 41.0 35.0 41.0 18 38.72460904747531 40.0 38.0 41.0 35.0 41.0 19 38.66766977355395 40.0 38.0 41.0 35.0 41.0 20 38.50594089291758 40.0 37.0 41.0 34.0 41.0 21 38.488336214597794 40.0 37.0 41.0 35.0 41.0 22 38.45572456941103 40.0 37.0 41.0 35.0 41.0 23 38.38784258844262 40.0 37.0 41.0 34.0 41.0 24 38.34577917623277 40.0 37.0 41.0 34.0 41.0 25 38.28207121754004 40.0 37.0 41.0 34.0 41.0 26 38.29159615953363 40.0 37.0 41.0 34.0 41.0 27 38.243062860348616 40.0 37.0 41.0 34.0 41.0 28 38.168659160134276 40.0 37.0 41.0 34.0 41.0 29 38.14026727157976 40.0 37.0 41.0 34.0 41.0 30 38.013381872730434 40.0 36.0 41.0 34.0 41.0 31 37.99950911790622 40.0 36.0 41.0 34.0 41.0 32 37.811952521639974 40.0 36.0 41.0 34.0 41.0 33 37.712748604339886 40.0 36.0 41.0 33.0 41.0 34 37.56618523633991 40.0 36.0 41.0 33.0 41.0 35 37.478954573588105 40.0 36.0 41.0 33.0 41.0 36 37.40153545479769 40.0 36.0 41.0 33.0 41.0 37 37.27249139431857 40.0 35.0 41.0 33.0 41.0 38 37.12505602458679 40.0 35.0 41.0 32.0 41.0 39 37.092423036700296 40.0 35.0 41.0 33.0 41.0 40 37.03245005046024 40.0 35.0 41.0 32.0 41.0 41 36.99707300117995 40.0 35.0 41.0 32.0 41.0 42 36.90182663020114 40.0 35.0 41.0 32.0 41.0 43 36.880502224214204 39.0 35.0 41.0 32.0 41.0 44 36.89788432866538 39.0 35.0 41.0 32.0 41.0 45 36.80793704513371 39.0 35.0 41.0 32.0 41.0 46 36.75130571587988 39.0 35.0 41.0 32.0 41.0 47 36.68602754427848 39.0 35.0 41.0 31.0 41.0 48 36.6207676664197 39.0 35.0 41.0 31.0 41.0 49 36.616709504513985 39.0 35.0 41.0 31.0 41.0 50 36.517255572731344 39.0 35.0 41.0 31.0 41.0 51 35.733987639527896 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 5.0 11 3.0 12 12.0 13 6.0 14 12.0 15 8.0 16 19.0 17 30.0 18 61.0 19 129.0 20 290.0 21 506.0 22 761.0 23 1122.0 24 1354.0 25 1844.0 26 2461.0 27 2860.0 28 2996.0 29 3262.0 30 3721.0 31 4576.0 32 5786.0 33 8241.0 34 16032.0 35 24620.0 36 20723.0 37 31093.0 38 59993.0 39 135265.0 40 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55709629521222 19.456614864885466 23.750461154762014 13.235827685140297 2 30.969476890429625 27.844295858601566 24.452635975864457 16.733591275104352 3 30.60787057786884 26.650324256588032 26.533244303785892 16.208560861757235 4 27.791548900698515 23.767840210256082 30.689582628262002 17.7510282607834 5 27.710141746015776 27.808013269061316 25.028583972852086 19.453261012070822 6 25.26396346129806 36.137154286376344 23.76753531454566 14.831346937779932 7 75.14642616493028 5.973516758592724 14.007213832508592 4.872843243968401 8 75.3055817257707 8.84441476792863 10.224982544720579 5.625020961580091 9 68.41280440025488 8.201084818937682 12.303456602669057 11.082654178138368 10 37.732673539015984 28.798314536512787 18.610529268463722 14.858482656007514 11 30.74354916900674 22.586064436659438 27.541839313862692 19.128547080471126 12 25.89418289474085 20.85303721861937 31.221930538659254 22.030849347980524 13 25.102978526195113 22.627835148987288 33.46230421884194 18.80688210597565 14 20.7972413036121 28.098883776804147 29.417862620090794 21.68601229949296 15 19.05476231854894 23.766925523124815 36.13258085072001 21.045731307606232 16 21.30672203572768 23.791622075669018 30.842640274893974 24.05901561370933 17 22.017433936721943 23.714483460932186 30.213945320003294 24.054137282342573 18 21.471975510776538 23.87577329174556 30.8115409124309 23.840710285047003 19 23.07786121757053 24.59014394126489 28.447074678106354 23.884920163058226 20 23.787963327143952 25.547516471990754 30.368222549476954 20.296297651388343 21 23.1364011939716 27.07748314688961 29.213582494107893 20.5725331650309 22 21.489049670560185 23.434894094474984 29.24010842091463 25.835947814050204 23 21.093599934142524 25.991749522075974 29.879779621380507 23.034870922400994 24 22.227811976913298 24.677344114445653 29.10290535122461 23.991938557416436 25 20.182876447111266 28.47055164780887 27.697945917598886 23.64862598748098 26 19.92493467609404 25.382567892652318 30.690497315393266 24.00200011586037 27 23.499531985084502 24.59868102115671 29.304136520103295 22.597650473655488 28 19.916397596202216 25.059683335315153 31.425295977510892 23.59862309097173 29 20.967982901448558 22.992185522941877 32.30431031065824 23.735521264951323 30 21.536308505675635 25.891133937636628 31.213088563057006 21.359468993630728 31 24.091944350434936 24.16725359090923 27.169256755726707 24.57154530292913 32 23.097374543037557 26.338111049115646 29.016924760885537 21.547589646961256 33 23.261103539534304 25.28256209963382 27.078092938310448 24.378241422521427 34 22.273546333476634 24.023037919879506 29.009912159545827 24.693503587098036 35 22.174455227589405 24.879185074745184 29.557199959753767 23.389159737911648 36 24.70935816403999 25.593555724264515 28.304383485628744 21.392702626066754 37 21.831447553364374 25.463670151624633 31.415844210487805 21.289038084523188 38 23.10103329156262 27.404941139883103 26.570746476167827 22.92327909238645 39 23.192197108978874 25.20511858918657 28.646476472722505 22.956207829112053 40 21.00853403093472 26.253045145907844 29.468170412310467 23.27025041084697 41 21.48295175635174 25.327686664776312 27.471713300465577 25.71764827840637 42 22.239702909619766 25.14871288275845 29.61970358039033 22.991880627231456 43 24.57154530292913 23.314460288858196 28.857159408624277 23.256834999588392 44 22.350684948213466 23.583073409740198 29.339809318222702 24.726432323823637 45 21.569847033822082 23.365377872498712 29.20291114424311 25.8618639494361 46 24.046819785292442 25.015473457303926 28.77575225394154 22.16195450346209 47 20.42435384976569 24.31909165469951 32.346385918696505 22.91016857683829 48 21.168299383195976 24.546543854674507 28.974239361426424 25.310917400703087 49 21.495147584768628 23.100423500141776 31.561584360069638 23.842844555019955 50 21.30580734859641 23.044932480844928 30.45847167976194 25.190788490796724 51 21.11036919821575 22.83912787630991 28.34066607516899 27.709836850305354 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 191.0 1 416.0 2 641.0 3 1979.0 4 3317.0 5 2198.0 6 1079.0 7 1034.0 8 989.0 9 1020.5 10 1052.0 11 1005.5 12 959.0 13 967.0 14 975.0 15 936.0 16 897.0 17 829.5 18 762.0 19 777.5 20 793.0 21 941.0 22 1089.0 23 1330.5 24 1572.0 25 1731.5 26 2255.0 27 2619.0 28 3213.0 29 3807.0 30 4192.5 31 4578.0 32 5363.5 33 6149.0 34 6860.5 35 7572.0 36 8596.0 37 9620.0 38 10552.5 39 11485.0 40 13017.5 41 14550.0 42 16345.0 43 18140.0 44 26539.0 45 34938.0 46 33484.0 47 32030.0 48 31299.5 49 30569.0 50 26737.5 51 22906.0 52 21003.5 53 19101.0 54 17301.0 55 15501.0 56 14892.5 57 14284.0 58 13734.5 59 13185.0 60 12826.5 61 12468.0 62 11296.0 63 10124.0 64 9386.5 65 8649.0 66 7251.5 67 5854.0 68 5014.5 69 4175.0 70 3709.0 71 3243.0 72 2880.5 73 2518.0 74 1909.5 75 1164.0 76 1027.0 77 889.5 78 752.0 79 559.5 80 367.0 81 251.5 82 136.0 83 112.0 84 88.0 85 59.0 86 30.0 87 18.0 88 6.0 89 3.5 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 327981.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.668476855390164 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75917812343252 22.091669178529628 2 9.518251043596521 6.028570257558594 3 3.573130138126717 3.3946676724168903 4 2.1883829182790486 2.7721101519300495 5 1.713546575424671 2.713270503223463 6 1.345218987051562 2.556062181412428 7 1.1615899566548231 2.575004926044385 8 1.1526073629105817 2.920105567654884 9 1.0168889380329396 2.8982991418738577 >10 8.445466993862535 43.04373893276113 >50 0.07185083578746769 1.5548611289535041 >100 0.043908844092341366 2.6100809073225593 >500 0.004989641374129701 0.9923300701044722 >1k 0.004989641374129701 3.8492293802141626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG 2934 0.8945640143788817 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC 2928 0.8927346401163482 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC 2622 0.7994365527271396 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2089 0.6369271390720804 No Hit GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC 1607 0.48996740664855587 No Hit CTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGCT 744 0.22684240855415405 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT 638 0.19452346324939557 No Hit CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC 633 0.1929989846972843 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT 613 0.18690107048883928 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCC 512 0.15610660373619203 No Hit TCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC 488 0.14878910668605802 No Hit GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT 481 0.14665483671310228 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC 429 0.1308002597711453 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA 409 0.12470234556270028 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.0489571042225007E-4 0.0 0.0 0.2326354270521768 0.0 2 3.0489571042225007E-4 0.0 0.0 1.1805561907549522 0.0 3 3.0489571042225007E-4 0.0 0.0 1.5897262341416118 0.0 4 3.0489571042225007E-4 0.0 0.0 2.236105140236782 0.0 5 3.0489571042225007E-4 0.0 0.0 4.306651909714282 0.0 6 3.0489571042225007E-4 0.0 0.0 5.097856278260021 0.0 7 3.0489571042225007E-4 0.0 0.0 6.033276317835484 0.0 8 3.0489571042225007E-4 0.0 0.0 7.36201182385565 0.0 9 3.0489571042225007E-4 0.0 0.0 7.891310777148676 0.0 10 3.0489571042225007E-4 0.0 0.0 9.748125653620178 0.0 11 3.0489571042225007E-4 0.0 0.0 11.025638680289408 0.0 12 3.0489571042225007E-4 0.0 0.0 12.972092895625051 0.0 13 3.0489571042225007E-4 0.0 0.0 13.443156768227428 0.0 14 3.0489571042225007E-4 0.0 0.0 13.651705434156247 0.0 15 3.0489571042225007E-4 0.0 0.0 14.076730054484864 0.0 16 3.0489571042225007E-4 0.0 0.0 14.717925733502856 0.0 17 3.0489571042225007E-4 0.0 0.0 15.464615328326946 0.0 18 3.0489571042225007E-4 0.0 0.0 16.301858949146446 0.0 19 3.0489571042225007E-4 0.0 0.0 16.974458886337928 0.0 20 3.0489571042225007E-4 0.0 0.0 17.479366182797175 0.0 21 3.0489571042225007E-4 0.0 0.0 18.026958878715536 0.0 22 6.097914208445001E-4 0.0 0.0 18.65108039794988 0.0 23 6.097914208445001E-4 0.0 0.0 19.205075903787108 0.0 24 6.097914208445001E-4 0.0 0.0 19.650528536714017 0.0 25 6.097914208445001E-4 0.0 0.0 20.05085660449843 0.0 26 6.097914208445001E-4 0.0 0.0 20.42618322402822 0.0 27 6.097914208445001E-4 0.0 0.0 20.839926703071214 0.0 28 6.097914208445001E-4 0.0 0.0 21.257024034928854 0.0 29 6.097914208445001E-4 0.0 0.0 21.656437415582 0.0 30 6.097914208445001E-4 0.0 0.0 22.09945088282553 0.0 31 9.146871312667503E-4 0.0 0.0 22.49520551495361 0.0 32 9.146871312667503E-4 0.0 0.0 22.863214637433266 0.0 33 9.146871312667503E-4 0.0 0.0 23.23244334275461 0.0 34 9.146871312667503E-4 0.0 0.0 23.632771410539025 0.0 35 9.146871312667503E-4 0.0 0.0 24.048649159554973 0.0 36 9.146871312667503E-4 0.0 0.0 24.44074504315799 0.0 37 9.146871312667503E-4 0.0 0.0 24.80692479137511 0.0 38 9.146871312667503E-4 0.0 0.0 25.182861202325743 0.0 39 9.146871312667503E-4 0.0 0.0 25.559712300407647 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATCG 20 7.0259057E-4 45.0 21 TAACGCC 20 7.0259057E-4 45.0 12 CCTAAGA 20 7.0259057E-4 45.0 31 CGCCTAA 20 7.0259057E-4 45.0 29 CTACGAA 40 6.7902874E-9 45.0 11 TACGAAT 40 6.7902874E-9 45.0 12 TGCGACG 20 7.0259057E-4 45.0 1 CATACGA 50 2.1827873E-11 45.0 18 GCGATAT 25 3.884689E-5 45.0 9 CGTAAGG 35 1.2083729E-7 45.0 2 CGATGCG 20 7.0259057E-4 45.0 10 CCGCCTA 20 7.0259057E-4 45.0 28 AACAGCG 20 7.0259057E-4 45.0 1 TGCGTTG 25 3.884689E-5 45.0 1 ATCGACC 20 7.0259057E-4 45.0 23 CCATTAC 40 6.7902874E-9 45.0 6 TAATCCA 75 0.0 42.0 42 CGTTTTT 920 0.0 40.842392 1 CAATAGG 50 1.0768417E-9 40.5 2 CATTACA 45 1.9210347E-8 40.000004 7 >>END_MODULE