FastQCFastQC Report
Sat 18 Jun 2016
SRR3551489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences672660
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260833.8775904617488774No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC34700.5158623970505158No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT17630.2620937769452621No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC17210.25584990931525586No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG17190.25555258228525557No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC13800.20515565070020514No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC13600.2021823804002022No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC12990.1931139059851931No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT11790.17527428418517527No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG11400.16947640710016948No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT10560.156988671840157No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC9870.14673088930514672No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT7610.11313293491511313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG406.8084773E-945.021
AATACGG207.031199E-445.02
CGTTTTT108850.044.049151
TAACGCC501.0804797E-940.512
ATAACGC501.0804797E-940.511
GTTTTTT122800.039.4299662
TTAACGG356.244427E-638.5714262
TCGTAAC356.244427E-638.57142634
ACAACGA1550.037.74193613
ACGTTTG301.1394186E-437.4999961
CGGTCTA301.1394186E-437.49999631
ATACCGG301.1394186E-437.4999962
AGTAACG301.1394186E-437.4999961
CGTAAGG552.744855E-936.8181842
AGTACGG552.744855E-936.8181842
CAACGAG1600.036.562514
TACGGGA1850.036.4864854
CGGGTAT504.8738002E-836.06
ATAGGTT250.002106488436.016
GACGTAG250.002106488436.01
Produced by FastQC (version 0.11.5)