##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	672660
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.364328189575716	33.0	31.0	34.0	31.0	34.0
2	32.54086462700324	34.0	31.0	34.0	31.0	34.0
3	32.45063181993875	34.0	31.0	34.0	31.0	34.0
4	35.97494573781702	37.0	35.0	37.0	35.0	37.0
5	36.08615199357774	37.0	35.0	37.0	35.0	37.0
6	36.105821663247404	37.0	36.0	37.0	35.0	37.0
7	36.24747718015045	37.0	37.0	37.0	35.0	37.0
8	36.16692831445307	37.0	37.0	37.0	35.0	37.0
9	38.03396961317753	39.0	39.0	39.0	35.0	39.0
10	37.824296078256474	39.0	38.0	39.0	35.0	39.0
11	37.765710760265215	39.0	38.0	39.0	35.0	39.0
12	37.62782089019713	39.0	37.0	39.0	35.0	39.0
13	37.576407100169476	39.0	37.0	39.0	35.0	39.0
14	38.82371777718313	40.0	38.0	41.0	35.0	41.0
15	38.88420004162578	41.0	38.0	41.0	35.0	41.0
16	38.94659857877679	41.0	38.0	41.0	35.0	41.0
17	38.86742336395802	40.0	38.0	41.0	35.0	41.0
18	38.58428775309964	40.0	38.0	41.0	35.0	41.0
19	38.27550173936312	40.0	37.0	41.0	35.0	41.0
20	37.94118722683079	40.0	35.0	41.0	34.0	41.0
21	37.84365652781494	40.0	35.0	41.0	34.0	41.0
22	37.85809918829721	40.0	35.0	41.0	34.0	41.0
23	37.81282222816876	39.0	35.0	41.0	34.0	41.0
24	37.73112716677073	40.0	35.0	41.0	34.0	41.0
25	37.635535039990486	39.0	35.0	41.0	34.0	41.0
26	37.64804210150745	39.0	35.0	41.0	34.0	41.0
27	37.632928968572536	39.0	35.0	41.0	34.0	41.0
28	37.48630214372788	39.0	35.0	41.0	34.0	41.0
29	37.36796449915262	39.0	35.0	41.0	34.0	41.0
30	37.1005708678976	39.0	35.0	41.0	33.0	41.0
31	36.80971813397556	39.0	35.0	41.0	32.0	41.0
32	36.32785508280558	39.0	35.0	41.0	31.0	41.0
33	35.8508890078197	39.0	35.0	41.0	28.0	41.0
34	35.341471174144445	39.0	35.0	41.0	23.0	41.0
35	35.08216929801088	39.0	35.0	41.0	21.0	41.0
36	34.90466654773586	39.0	35.0	41.0	21.0	41.0
37	34.808710195343856	39.0	35.0	41.0	20.0	41.0
38	34.652888532096455	38.0	35.0	41.0	18.0	41.0
39	34.643793298248745	38.0	35.0	41.0	18.0	41.0
40	34.56173698450926	38.0	35.0	41.0	18.0	41.0
41	34.43444830969583	38.0	35.0	41.0	18.0	41.0
42	34.266003627389765	38.0	34.0	41.0	18.0	41.0
43	34.246105015907	38.0	34.0	41.0	17.0	41.0
44	34.20884993904796	38.0	34.0	41.0	18.0	41.0
45	34.12654387060328	37.0	34.0	41.0	18.0	41.0
46	34.10611007046651	37.0	34.0	40.0	18.0	41.0
47	34.05632711919841	37.0	34.0	40.0	18.0	41.0
48	34.007229506734454	37.0	34.0	40.0	18.0	41.0
49	33.97249130318437	37.0	34.0	40.0	18.0	41.0
50	33.88498498498498	37.0	34.0	40.0	18.0	41.0
51	33.08258555585288	35.0	33.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	6.0
10	15.0
11	16.0
12	12.0
13	17.0
14	16.0
15	36.0
16	62.0
17	119.0
18	186.0
19	409.0
20	698.0
21	1108.0
22	1820.0
23	2717.0
24	4571.0
25	8865.0
26	14614.0
27	18287.0
28	17767.0
29	14923.0
30	13279.0
31	13297.0
32	14685.0
33	19931.0
34	38912.0
35	48629.0
36	49792.0
37	60514.0
38	112718.0
39	214381.0
40	250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.31442333422532	16.725388755091725	20.63226592929563	27.327921981387327
2	42.71875836232272	20.9025361995659	20.935093509350935	15.443611928760442
3	26.664436713941665	21.16998186305117	37.52638777391253	14.63919364909464
4	23.867481342728865	21.08896024737609	38.672434811048674	16.37112359884637
5	22.961377218802962	23.87937442392888	35.700799809710695	17.45844854755746
6	22.538578182142537	27.982636101447984	35.21913002111022	14.259655695299259
7	66.25115214224125	4.8825558231498825	23.836707995123838	5.029584039485029
8	66.997591651057	5.088454791425089	22.818214253857818	5.095739303660095
9	61.32474058216633	5.9520411500609525	24.947521779204948	7.7756964885677755
10	34.28834775369429	21.46745755656647	30.101983171290104	14.142211518449141
11	27.63074956144263	19.054202717569055	36.095501442036095	17.21954627895222
12	23.76252490113876	17.300865221657304	39.20792079207921	19.728689085124728
13	22.654981714387652	17.17301459875717	41.802247792346805	18.36975589450837
14	18.47159040228347	20.394850295840396	41.320132013201324	19.813427288674816
15	17.788184223827788	19.354800344899356	43.787797698688784	19.06921773258407
16	21.502988136651503	19.913923824814912	39.925519578984925	18.657568459548656
17	20.844854755745846	20.625724734635625	38.8031100407338	19.726310468884726
18	22.009484732257008	19.6023250973746	38.696369636963695	19.69182053340469
19	21.075729194541076	22.35958731008236	36.54342461273154	20.02125888264502
20	21.818600778996817	22.711027859542714	36.63737995421164	18.832991407248834
21	20.82731246097583	23.907025837718905	37.40998424166741	17.855677459637857
22	20.133053845925133	20.155353373175156	38.32218356970832	21.389409211191392
23	19.642166919394644	22.126631582077128	37.99096125828799	20.24024024024024
24	20.34014212232034	20.976570630035976	37.92138673326792	20.76190051437576
25	21.415276662801418	22.293134718877294	35.65025421461065	20.64133440371064
26	19.84152469300984	21.17994231855618	36.596497487586596	22.382035500847383
27	21.59203758213659	19.95882020634496	36.746498974221744	21.702643237296705
28	20.165165165165167	21.67900573841168	36.616269735081616	21.53955936134154
29	22.76900663039277	21.38881455713139	35.16888175304017	20.673297059435676
30	24.178039425564176	19.87987987987988	36.48945975678649	19.452620937769453
31	24.093152558499092	20.046085689650045	35.4995094104005	20.36125234145036
32	24.786073201914785	21.32132132132132	34.58017423363958	19.312431243124312
33	23.94181310022894	21.807897005916807	33.64032349180864	20.60996640204561
34	22.24288645080724	22.473463562572473	36.13028870454613	19.15336128207415
35	21.72880801593673	24.233490916659235	33.82436892337882	20.213332144025216
36	21.94020753426694	26.061160170071062	32.55835042963756	19.44028186602444
37	21.149614961496148	26.3040763535813	32.91499420212291	19.63131448279963
38	20.777361519935777	25.596289358665597	32.75607290458776	20.870276216810872
39	21.319834686171323	24.295929592959293	32.286147533672285	22.0980881871971
40	22.100764130467102	22.633425504712633	34.02491600511402	21.240894359706243
41	19.88315047720988	24.808521392679808	32.600273540867605	22.708054589242707
42	21.142627776291143	24.716796003924717	32.15725626616715	21.983319953616984
43	21.78842208545179	23.367228614753365	32.998245770523	21.846103529271847
44	22.59046174887759	22.974459608122974	32.47272024499747	21.962358398001964
45	21.59069961050159	21.87895816608688	32.60800404364761	23.922338179763923
46	21.983765944161984	22.527725745547528	32.52133321440252	22.967175095887967
47	20.674783694585674	23.147503939583146	34.587607409389584	21.590104956441593
48	20.662295959325665	22.87976094906788	33.80638063806381	22.651562453542653
49	21.874498260636873	21.756162102696756	34.024618678084025	22.344720958582347
50	20.134837808105136	21.68926352094669	35.048166978860046	23.127731692088126
51	20.007284512235007	21.58891564832159	33.7980554812238	24.605744358219606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	736.0
2	1178.0
3	20164.0
4	39150.0
5	24504.0
6	9858.0
7	9508.0
8	9158.0
9	9021.5
10	8885.0
11	8469.0
12	8053.0
13	7673.0
14	7293.0
15	6747.5
16	6202.0
17	5795.0
18	5388.0
19	4924.0
20	4460.0
21	4263.5
22	4067.0
23	4001.5
24	3936.0
25	4128.0
26	4663.5
27	5007.0
28	5405.0
29	5803.0
30	6346.0
31	6889.0
32	7753.5
33	8618.0
34	9786.5
35	10955.0
36	12004.5
37	13054.0
38	14470.0
39	15886.0
40	17675.0
41	19464.0
42	22862.0
43	26260.0
44	37417.0
45	48574.0
46	49905.5
47	51237.0
48	52851.0
49	54465.0
50	49126.0
51	43787.0
52	40436.0
53	37085.0
54	35525.0
55	33965.0
56	33184.0
57	32403.0
58	30943.5
59	29484.0
60	28411.0
61	27338.0
62	25546.0
63	23754.0
64	21249.5
65	18745.0
66	16184.0
67	13623.0
68	11945.0
69	10267.0
70	9139.0
71	8011.0
72	6888.5
73	5766.0
74	5032.0
75	3508.5
76	2719.0
77	2098.0
78	1477.0
79	1125.5
80	774.0
81	575.5
82	377.0
83	277.0
84	177.0
85	138.5
86	100.0
87	71.5
88	43.0
89	26.5
90	10.0
91	6.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	672660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.62656710020238
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.76294257983608	29.872449446075166
2	8.768095192492579	7.299714057425084
3	4.44867514787907	5.555492236505724
4	3.1311865482030883	5.213621878081077
5	2.4895588565220557	5.181589439545923
6	2.0191625490795513	5.043048320128538
7	1.622188514710005	4.726829733972756
8	1.2914779439965245	4.300783463536219
9	1.0626371593482409	3.9810543315100206
>10	3.3296262182346013	18.737205351293852
>50	0.04545702069421468	1.2771737794103444
>100	0.022191613311726494	1.7384580033926467
>500	0.0028634339757065332	0.7895173945815422
>1k	0.003579292469633167	2.396860978794729
>5k	0.0	0.0
>10k+	3.5792924696331665E-4	3.886201585746437
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26083	3.8775904617488774	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	3470	0.5158623970505158	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTT	1763	0.2620937769452621	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGC	1721	0.25584990931525586	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCG	1719	0.25555258228525557	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	1380	0.20515565070020514	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTC	1360	0.2021823804002022	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCC	1299	0.1931139059851931	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCT	1179	0.17527428418517527	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTG	1140	0.16947640710016948	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCT	1056	0.156988671840157	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGC	987	0.14673088930514672	No Hit
CTGTCTCTTATACACATCTGACGCGGTATTCATCGTATGCCGTCTTCTGCT	761	0.11313293491511313	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9732703000029734E-4	0.0	0.0	0.1172955133351173	0.0
2	2.9732703000029734E-4	0.0	0.0	0.5484197068355484	0.0
3	2.9732703000029734E-4	0.0	0.0	0.9496625338209497	0.0
4	2.9732703000029734E-4	0.0	0.0	1.4423334225314424	0.0
5	2.9732703000029734E-4	0.0	0.0	2.8733684179228733	0.0
6	2.9732703000029734E-4	0.0	0.0	4.363868819314364	0.0
7	2.9732703000029734E-4	0.0	0.0	5.609223084470609	0.0
8	2.9732703000029734E-4	0.0	0.0	7.282876936342283	0.0
9	2.9732703000029734E-4	0.0	0.0	8.428477982933428	0.0
10	2.9732703000029734E-4	0.0	0.0	10.062438676300063	0.0
11	2.9732703000029734E-4	0.0	0.0	11.245651592186245	0.0
12	2.9732703000029734E-4	0.0	0.0	12.393779918532394	0.0
13	2.9732703000029734E-4	0.0	0.0	12.872625100347873	0.0
14	2.9732703000029734E-4	0.0	0.0	13.196860226563198	0.0
15	2.9732703000029734E-4	0.0	0.0	13.494781910623495	0.0
16	2.9732703000029734E-4	0.0	0.0	13.824220259863825	0.0
17	2.9732703000029734E-4	0.0	0.0	14.18933785270419	0.0
18	2.9732703000029734E-4	0.0	0.0	14.614515505604615	0.0
19	2.9732703000029734E-4	0.0	0.0	14.946927125144947	0.0
20	2.9732703000029734E-4	0.0	0.0	15.268486308090269	0.0
21	2.9732703000029734E-4	0.0	0.0	15.581720334195582	0.0
22	2.9732703000029734E-4	0.0	0.0	15.94966253382095	0.0
23	2.9732703000029734E-4	0.0	0.0	16.273451669491273	0.0
24	2.9732703000029734E-4	0.0	0.0	16.563494187256563	0.0
25	2.9732703000029734E-4	0.0	0.0	16.82975054262183	0.0
26	2.9732703000029734E-4	0.0	0.0	17.086046442482086	0.0
27	2.9732703000029734E-4	0.0	0.0	17.383224808967384	0.0
28	2.9732703000029734E-4	0.0	0.0	17.657063003597656	0.0
29	2.9732703000029734E-4	0.0	0.0	17.957809294442956	0.0
30	2.9732703000029734E-4	0.0	0.0	18.353848898403353	0.0
31	2.9732703000029734E-4	0.0	0.0	18.63675556744864	0.0
32	2.9732703000029734E-4	0.0	0.0	18.93973181101894	0.0
33	2.9732703000029734E-4	0.0	0.0	19.21044807183421	0.0
34	2.9732703000029734E-4	0.0	0.0	19.48993548003449	0.0
35	2.9732703000029734E-4	0.0	0.0	19.784883893794785	0.0
36	2.9732703000029734E-4	0.0	0.0	20.071804477745072	0.0
37	4.45990545000446E-4	0.0	0.0	20.372402105075373	0.0
38	4.45990545000446E-4	0.0	0.0	20.767401064430768	0.0
39	4.45990545000446E-4	0.0	0.0	21.32920048761633	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	40	6.8084773E-9	45.0	21
AATACGG	20	7.031199E-4	45.0	2
CGTTTTT	10885	0.0	44.04915	1
TAACGCC	50	1.0804797E-9	40.5	12
ATAACGC	50	1.0804797E-9	40.5	11
GTTTTTT	12280	0.0	39.429966	2
TTAACGG	35	6.244427E-6	38.571426	2
TCGTAAC	35	6.244427E-6	38.571426	34
ACAACGA	155	0.0	37.741936	13
ACGTTTG	30	1.1394186E-4	37.499996	1
CGGTCTA	30	1.1394186E-4	37.499996	31
ATACCGG	30	1.1394186E-4	37.499996	2
AGTAACG	30	1.1394186E-4	37.499996	1
CGTAAGG	55	2.744855E-9	36.818184	2
AGTACGG	55	2.744855E-9	36.818184	2
CAACGAG	160	0.0	36.5625	14
TACGGGA	185	0.0	36.486485	4
CGGGTAT	50	4.8738002E-8	36.0	6
ATAGGTT	25	0.0021064884	36.0	16
GACGTAG	25	0.0021064884	36.0	1
>>END_MODULE