##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	392156
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.421793877946534	33.0	31.0	34.0	31.0	34.0
2	32.52438570364855	34.0	31.0	34.0	31.0	34.0
3	32.50281520619345	34.0	31.0	34.0	31.0	34.0
4	36.030013566029844	37.0	35.0	37.0	35.0	37.0
5	36.10025092055203	37.0	35.0	37.0	35.0	37.0
6	36.089839247646346	37.0	36.0	37.0	35.0	37.0
7	36.26023062250737	37.0	37.0	37.0	35.0	37.0
8	36.11551270412795	37.0	37.0	37.0	35.0	37.0
9	38.023942002672406	39.0	39.0	39.0	35.0	39.0
10	37.672362529197564	39.0	37.0	39.0	35.0	39.0
11	37.672056528524365	39.0	37.0	39.0	35.0	39.0
12	37.58934199655239	39.0	37.0	39.0	35.0	39.0
13	37.586210589663295	39.0	37.0	39.0	35.0	39.0
14	38.88699395138669	40.0	38.0	41.0	35.0	41.0
15	38.951521843348054	40.0	38.0	41.0	35.0	41.0
16	38.949469088832	40.0	38.0	41.0	35.0	41.0
17	38.903581737879826	40.0	38.0	41.0	35.0	41.0
18	38.77693315925295	40.0	38.0	41.0	35.0	41.0
19	38.65685849508869	40.0	37.0	41.0	35.0	41.0
20	38.54952365895205	40.0	37.0	41.0	35.0	41.0
21	38.442492273483005	40.0	37.0	41.0	35.0	41.0
22	38.44163037158682	40.0	37.0	41.0	35.0	41.0
23	38.42542508593519	40.0	37.0	41.0	35.0	41.0
24	38.360960944114076	40.0	37.0	41.0	35.0	41.0
25	38.19016411836106	40.0	36.0	41.0	34.0	41.0
26	38.19866838707045	40.0	36.0	41.0	34.0	41.0
27	38.20141729311805	40.0	36.0	41.0	34.0	41.0
28	38.13204694050327	40.0	36.0	41.0	34.0	41.0
29	38.07644916818817	40.0	36.0	41.0	34.0	41.0
30	37.901796733952814	40.0	36.0	41.0	34.0	41.0
31	37.847468864431505	40.0	36.0	41.0	34.0	41.0
32	37.68961841716052	40.0	36.0	41.0	33.0	41.0
33	37.51177847591265	40.0	36.0	41.0	33.0	41.0
34	37.367705708952556	40.0	35.0	41.0	33.0	41.0
35	37.216128275482205	40.0	35.0	41.0	33.0	41.0
36	37.08502228704903	40.0	35.0	41.0	33.0	41.0
37	37.03894623568172	40.0	35.0	41.0	32.0	41.0
38	36.93002274605004	40.0	35.0	41.0	32.0	41.0
39	36.89679872295719	40.0	35.0	41.0	32.0	41.0
40	36.80211191464621	40.0	35.0	41.0	32.0	41.0
41	36.689549567009045	39.0	35.0	41.0	31.0	41.0
42	36.563046338701945	39.0	35.0	41.0	31.0	41.0
43	36.43924866634707	39.0	35.0	41.0	31.0	41.0
44	36.452059894531764	39.0	35.0	41.0	31.0	41.0
45	36.41807341976153	39.0	35.0	41.0	31.0	41.0
46	36.33719744183437	39.0	35.0	41.0	31.0	41.0
47	36.29659115250053	39.0	35.0	41.0	31.0	41.0
48	36.23632941992472	39.0	35.0	41.0	31.0	41.0
49	36.187392262262975	39.0	35.0	41.0	31.0	41.0
50	36.102431175348585	38.0	35.0	41.0	31.0	41.0
51	35.28864788502535	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	3.0
10	6.0
11	8.0
12	4.0
13	6.0
14	14.0
15	27.0
16	25.0
17	48.0
18	82.0
19	146.0
20	238.0
21	381.0
22	627.0
23	991.0
24	1477.0
25	2550.0
26	3692.0
27	4485.0
28	4531.0
29	4581.0
30	4719.0
31	5531.0
32	6844.0
33	9714.0
34	19190.0
35	29795.0
36	27304.0
37	40558.0
38	72020.0
39	152371.0
40	184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.76682748702047	20.65887044951499	24.482603861728496	15.091698201736046
2	35.71180856597885	22.808525178755392	24.331388529054763	17.148277726211
3	32.09513560929834	22.370179214394273	28.953273697202135	16.581411479105252
4	28.526912759208074	24.331898530176765	28.549097808015176	18.592090902599985
5	25.188445414579913	28.965258723569193	26.69294872448719	19.153347137363703
6	25.626026377258032	33.806189373616625	26.236242719733983	14.331541529391364
7	77.8514162731158	6.088393394465467	11.524240353328777	4.535949979089954
8	78.17577698670937	5.897397974275544	11.192484623466171	4.734340415548914
9	72.10523363151398	7.941482471261437	13.638195004029008	6.315088893195566
10	39.391211660665654	23.24993114984853	21.509042319893105	15.849814869592713
11	31.134038494884685	23.220096084211388	25.259590571099256	20.38627484980467
12	26.40785809728781	21.307081875580124	31.455339201746245	20.829720825385817
13	24.579249074347963	22.579534674976284	32.76731708809759	20.073899162578158
14	21.780617917359418	25.116025255255565	31.85747508644519	21.24588174093983
15	20.971501137302504	23.565111843246058	34.06552494415488	21.397862075296565
16	23.673997082793584	22.567549648609226	31.88629014983833	21.872163118758863
17	23.468721631187588	22.81974520343945	29.852915676414487	23.858617488958476
18	23.668897071573557	22.806740174828384	31.20747865645304	22.316884097145014
19	24.39870867715909	24.560889033955874	28.943073674762083	22.09732861412295
20	24.79523454951601	24.995919991023978	29.308744479237852	20.900100980222156
21	24.85923969032732	24.38187864013301	29.84705066351146	20.911831006028216
22	23.444496577892473	23.265741184630606	28.94766368486011	24.342098552616818
23	22.980140556309223	24.404318689501117	29.324044512897927	23.291496241291732
24	22.851875274125604	24.67385428247942	30.046461102214423	22.427809341180552
25	23.15277593610706	24.94466487826273	28.935933659054047	22.96662552657616
26	21.777557910627404	24.980874957924907	29.23377431430349	24.007792817144196
27	23.57837187241812	24.472658839849448	29.836085639388404	22.112883648344024
28	21.602117524658553	24.583584083884986	31.207733657014046	22.606564734442415
29	22.53949958689909	24.1941982272361	30.128826283417826	23.137475902446987
30	22.71085996389192	24.932169850773672	29.409979701955347	22.94699048337906
31	23.79537734983017	25.130560287232633	28.269617193157824	22.804445169779374
32	24.04757290466039	25.078540172788383	28.056946725282796	22.816940197268433
33	23.083670784075725	25.964157121145664	26.801068962351714	24.15110313242689
34	22.20825385815849	24.78120951866094	28.981323758912268	24.029212864268302
35	23.102285825028815	24.419363722600192	28.08678179091994	24.391568661451053
36	22.888850355470783	26.84110405042891	27.585450687991514	22.684594906108792
37	22.244718938381663	26.789848937667664	28.889523556951822	22.075908566998848
38	22.52878956333704	26.307897877375332	27.576270667795473	23.58704189149216
39	23.045930701047542	24.86816470996236	28.27675720886586	23.809147380124237
40	21.985128367282407	24.071797957955507	30.014841032650274	23.928232642111812
41	20.553300217260478	25.252960556513226	28.527167759769075	25.666571466457228
42	21.32008690419119	26.383378043431694	28.860708493558683	23.43582655881843
43	22.505074511163926	24.426758738869225	29.401819684003307	23.666347065963546
44	22.701679943695876	23.79333734534216	29.012688827915422	24.492293883046543
45	23.232336111139446	23.948887687552915	28.2229520904946	24.595824110813044
46	22.975550546211203	24.571599057517926	29.01039382286641	23.44245657340446
47	21.544487397872274	24.55808402778486	30.508522118748658	23.388906455594203
48	21.24613674150083	25.07446016381236	29.438539764787482	24.240863329899327
49	22.05576352267975	24.239843327655322	30.108171237976723	23.596221911688207
50	21.263986780770917	23.467446628382582	30.209661461255216	25.058905129591285
51	21.47640224808495	24.19674823284611	28.465712624567775	25.86113689450117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	228.0
1	577.5
2	927.0
3	3806.5
4	6686.0
5	4330.5
6	1975.0
7	1958.0
8	1941.0
9	1960.5
10	1980.0
11	2006.0
12	2032.0
13	1900.5
14	1769.0
15	1616.0
16	1463.0
17	1430.5
18	1398.0
19	1331.0
20	1264.0
21	1373.0
22	1482.0
23	1536.0
24	1590.0
25	1831.0
26	2584.0
27	3096.0
28	3433.5
29	3771.0
30	4671.5
31	5572.0
32	6171.5
33	6771.0
34	7399.5
35	8028.0
36	8540.0
37	9052.0
38	10259.0
39	11466.0
40	13158.0
41	14850.0
42	17187.5
43	19525.0
44	23399.0
45	27273.0
46	30152.5
47	33032.0
48	34306.0
49	35580.0
50	31489.0
51	27398.0
52	25473.5
53	23549.0
54	21946.5
55	20344.0
56	20483.5
57	20623.0
58	19782.5
59	18942.0
60	18434.5
61	17927.0
62	16170.0
63	14413.0
64	13830.0
65	13247.0
66	11114.5
67	8982.0
68	7810.0
69	6638.0
70	5966.0
71	5294.0
72	4532.5
73	3771.0
74	3258.0
75	2167.0
76	1589.0
77	1266.0
78	943.0
79	732.0
80	521.0
81	373.0
82	225.0
83	191.5
84	158.0
85	86.5
86	15.0
87	9.5
88	4.0
89	2.0
90	0.0
91	1.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	392156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.662904850525535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61984031372012	24.82551710204313
2	8.312276833140846	5.762553219167777
3	3.3429487395218285	3.4762894223468823
4	2.1474673763141405	2.977498293391391
5	1.6990782519085121	2.9447493889751004
6	1.5083792758270422	3.137088438989842
7	1.3062883830961929	3.1695824951427127
8	1.155750363639414	3.2049331908634633
9	1.0728350127793154	3.3468820171453584
>10	7.730962996594941	41.442482460217946
>50	0.06206018503860771	1.4884160371028263
>100	0.03915702151245486	2.599350099207448
>500	0.0022164351799502753	0.5121872116664922
>1k	7.388117266500918E-4	1.1124706237396211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4344	1.1077224369893615	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	746	0.1902304185069207	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	724	0.18462040616489356	No Hit
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	530	0.13515029733065415	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	422	0.10761023674252083	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	417	0.10633523393751465	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	406	0.10353022776650109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10914024010852824	0.0
2	0.0	0.0	0.0	0.5678862493497485	0.0
3	0.0	0.0	0.0	0.8313018288640235	0.0
4	0.0	0.0	0.0	1.1571925458236008	0.0
5	0.0	0.0	0.0	2.1216046675302684	0.0
6	0.0	0.0	0.0	2.7364110201042444	0.0
7	0.0	0.0	0.0	3.3071022756250064	0.0
8	0.0	0.0	0.0	4.228929303644468	0.0
9	0.0	0.0	0.0	4.628260182172401	0.0
10	0.0	0.0	0.0	5.511582125480676	0.0
11	0.0	0.0	0.0	6.7853099276818405	0.0
12	0.0	0.0	0.0	7.715806974775345	0.0
13	0.0	0.0	0.0	8.084282785422127	0.0
14	0.0	0.0	0.0	8.222748090045798	0.0
15	0.0	0.0	0.0	8.512683727904202	0.0
16	0.0	0.0	0.0	9.038494884688747	0.0
17	0.0	0.0	0.0	9.686706310753884	0.0
18	0.0	0.0	0.0	10.419832923632432	0.0
19	0.0	0.0	0.0	10.845938861065495	0.0
20	0.0	0.0	0.0	11.26872979120554	0.0
21	0.0	0.0	0.0	11.748895847570864	0.0
22	0.0	0.0	0.0	12.290772039698487	0.0
23	5.100011220024684E-4	0.0	0.0	12.83672824080213	0.0
24	5.100011220024684E-4	0.0	0.0	13.287569232652311	0.0
25	5.100011220024684E-4	0.0	0.0	13.686390110058243	0.0
26	5.100011220024684E-4	0.0	0.0	14.036505880312937	0.0
27	5.100011220024684E-4	0.0	0.0	14.40498169095972	0.0
28	5.100011220024684E-4	0.0	0.0	14.772437499362498	0.0
29	5.100011220024684E-4	0.0	0.0	15.168963371719418	0.0
30	5.100011220024684E-4	0.0	0.0	15.582319281102418	0.0
31	5.100011220024684E-4	0.0	0.0	15.955385101847224	0.0
32	5.100011220024684E-4	0.0	0.0	16.34145595120309	0.0
33	5.100011220024684E-4	0.0	0.0	16.711206764654882	0.0
34	5.100011220024684E-4	0.0	0.0	17.071522557349628	0.0
35	5.100011220024684E-4	0.0	0.0	17.462693417925518	0.0
36	5.100011220024684E-4	0.0	0.0	17.83269923193831	0.0
37	5.100011220024684E-4	0.0	0.0	18.191740021828046	0.0
38	5.100011220024684E-4	0.0	0.0	18.55970583135283	0.0
39	5.100011220024684E-4	0.0	0.0	18.93991166780567	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	50	2.1827873E-11	45.0	38
CGCATCG	55	1.8189894E-12	45.0	21
ATAGCCG	20	7.0275966E-4	45.0	1
TCCTCGG	20	7.0275966E-4	45.0	16
CGTCTAT	25	3.886089E-5	45.0	36
GTCGTTT	20	7.0275966E-4	45.0	9
CGGTCTA	25	3.886089E-5	45.0	31
CCGGTAT	35	1.2091914E-7	45.0	42
CGGTATT	35	1.2091914E-7	45.0	43
AGGGCGT	20	7.0275966E-4	45.0	6
TCAAGCG	50	2.1827873E-11	45.0	17
CGTTTAT	50	2.1827873E-11	45.0	39
TGCAACG	20	7.0275966E-4	45.0	1
ACGAACG	20	7.0275966E-4	45.0	1
TCGGCGT	120	0.0	43.125004	4
GCGTCCC	130	0.0	41.53846	7
CGTTTTT	1875	0.0	41.4	1
AACGCCG	55	6.002665E-11	40.909092	13
TGGTCAA	55	6.002665E-11	40.909092	14
ACACGAC	50	1.0768417E-9	40.5	26
>>END_MODULE