FastQCFastQC Report
Sat 18 Jun 2016
SRR3551487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457100
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG78571.7188798949901556No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC68971.508860205644279No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66851.4624808575803983No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC60681.3274994530737256No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC22130.4841391380441916No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT16000.3500328155764603No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAGT14660.32071756727193174No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTA12430.27193174360096256No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT11830.25880551301684535No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC10880.23802231459199302No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC8400.18376722817764166No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT5970.13060599431196673No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5140.11244804200393786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGCG551.8189894E-1245.036
CGTATGG207.028824E-445.02
CGACGGT253.887106E-545.028
CCGCTCG253.887106E-545.019
GGTATCG207.028824E-445.01
ACAGACG207.028824E-445.043
TATACGG253.887106E-545.02
ACGGTCG207.028824E-445.040
ACCGAAG302.162622E-644.99999617
CTACGAA302.162622E-644.99999611
CGAATAT302.162622E-644.99999614
CGTAAGC302.162622E-644.99999643
GATTGCG302.162622E-644.9999961
TATAGCG302.162622E-644.9999961
TAGTATG302.162622E-644.9999961
CGTTTTT31200.043.7019231
CGATGAA7200.041.87519
CAGTCGA650.041.5384641
CGGCGAT603.6379788E-1241.24999631
CCGATGA7050.040.85106318
Produced by FastQC (version 0.11.5)