##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457100
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20450010938526	33.0	31.0	34.0	30.0	34.0
2	32.29085758039816	34.0	31.0	34.0	30.0	34.0
3	32.2955370816014	34.0	31.0	34.0	30.0	34.0
4	35.88681907678845	37.0	35.0	37.0	35.0	37.0
5	35.94117479763728	37.0	35.0	37.0	35.0	37.0
6	35.93036972216145	37.0	35.0	37.0	35.0	37.0
7	36.114686064318526	37.0	35.0	37.0	35.0	37.0
8	35.87504703565959	37.0	35.0	37.0	35.0	37.0
9	37.808689564646684	39.0	38.0	39.0	35.0	39.0
10	37.45196674688252	39.0	37.0	39.0	34.0	39.0
11	37.48343032159265	39.0	37.0	39.0	35.0	39.0
12	37.425138919273685	39.0	37.0	39.0	35.0	39.0
13	37.36738131699847	39.0	37.0	39.0	34.0	39.0
14	38.67339969372129	40.0	38.0	41.0	35.0	41.0
15	38.638031065412385	40.0	38.0	41.0	34.0	41.0
16	38.744095383942245	40.0	38.0	41.0	35.0	41.0
17	38.62391599212426	40.0	38.0	41.0	34.0	41.0
18	38.49164077882301	40.0	38.0	41.0	34.0	41.0
19	38.3511551082914	40.0	37.0	41.0	34.0	41.0
20	38.070319404944215	40.0	36.0	41.0	34.0	41.0
21	38.13275213301247	40.0	36.0	41.0	34.0	41.0
22	38.14725224239773	40.0	36.0	41.0	34.0	41.0
23	38.04709035222052	40.0	36.0	41.0	34.0	41.0
24	38.00811201050099	40.0	36.0	41.0	34.0	41.0
25	37.9405797418508	40.0	36.0	41.0	34.0	41.0
26	37.91651061036972	40.0	36.0	41.0	34.0	41.0
27	37.84654123824109	40.0	36.0	41.0	34.0	41.0
28	37.79577772916211	40.0	36.0	41.0	33.0	41.0
29	37.69296434040691	40.0	36.0	41.0	33.0	41.0
30	37.56767884489171	40.0	36.0	41.0	33.0	41.0
31	37.48303653467513	40.0	36.0	41.0	33.0	41.0
32	37.18430759133669	40.0	35.0	41.0	32.0	41.0
33	36.91997155983373	40.0	35.0	41.0	31.0	41.0
34	36.70048129512142	40.0	35.0	41.0	31.0	41.0
35	36.5090089695909	40.0	35.0	41.0	30.0	41.0
36	36.479085539269306	40.0	35.0	41.0	30.0	41.0
37	36.37843579085539	40.0	35.0	41.0	30.0	41.0
38	36.27215926493109	40.0	35.0	41.0	29.0	41.0
39	36.2550426602494	40.0	35.0	41.0	29.0	41.0
40	36.15577772916211	39.0	35.0	41.0	29.0	41.0
41	36.11705316123387	39.0	35.0	41.0	28.0	41.0
42	35.95259243054036	39.0	35.0	41.0	27.0	41.0
43	35.86183767228177	39.0	35.0	41.0	27.0	41.0
44	35.972638372347404	39.0	35.0	41.0	28.0	41.0
45	35.957114416976594	39.0	35.0	41.0	28.0	41.0
46	35.908750820389415	39.0	35.0	41.0	28.0	41.0
47	35.82637278494859	39.0	35.0	41.0	28.0	41.0
48	35.79680157514767	39.0	35.0	41.0	28.0	41.0
49	35.79759571209801	39.0	35.0	41.0	28.0	41.0
50	35.66535331437322	39.0	35.0	41.0	27.0	41.0
51	34.88374097571647	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	4.0
11	7.0
12	7.0
13	6.0
14	11.0
15	16.0
16	23.0
17	57.0
18	127.0
19	259.0
20	613.0
21	1307.0
22	1944.0
23	2448.0
24	3004.0
25	4431.0
26	6166.0
27	7046.0
28	6565.0
29	6015.0
30	6210.0
31	7449.0
32	9212.0
33	12443.0
34	21724.0
35	31326.0
36	30729.0
37	42844.0
38	83325.0
39	171567.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65696784073507	17.774447604462917	21.897615401443886	14.670969153358126
2	33.777510391599215	28.846860643185295	22.30824764821702	15.067381316998468
3	28.752789323999124	28.79960621308248	28.246773134981407	14.200831327936994
4	24.777072850579742	22.03019033034347	36.22467731349814	16.968059505578648
5	28.07481951432947	25.948807700721943	27.448260774447604	18.528112010500987
6	21.979654342594618	37.706628746444984	27.297965434259464	13.01575147670094
7	70.92430540363159	5.057974185079852	19.808575803981622	4.209144607306935
8	70.70947276307153	10.655873988186393	13.894990155327061	4.7396630934150075
9	65.81820170640998	6.15226427477576	14.784948588930213	13.24458542988405
10	35.70444104134763	21.77357252242398	24.42660249398381	18.095383942244585
11	30.011813607525706	23.27849485889302	27.164734193830668	19.544957339750603
12	25.37366003062787	19.308028877707283	32.48873331874863	22.82957777291621
13	24.792824327280684	20.490921023845985	35.730912273025595	18.985342375847736
14	20.180704441041346	28.045723036534675	30.2988405162984	21.474732006125574
15	17.8133887551958	23.000218770509736	38.105009844672935	21.081382629621526
16	19.387442572741197	22.901772041128858	32.69940931962371	25.011376066506237
17	20.29337125355502	24.043097790417853	33.15488952089258	22.508641435134543
18	20.21964559177423	23.55217676657187	32.733756289652156	23.49442135200175
19	21.598993655655217	24.961277619776855	30.36009625902428	23.079632465543646
20	23.658936775322687	24.162327718223583	32.88120761321374	19.29752789323999
21	21.251804856705316	28.737037847298186	30.49748413913804	19.513673156858456
22	19.138044191642965	22.908772697440387	32.52679938744257	25.42638372347408
23	20.984467293808795	27.32378035440823	31.197768540800702	20.49398381098228
24	21.191642966528114	24.87158171078539	30.427477576022753	23.509297746663748
25	19.78779260555677	29.507547582585868	29.025158608619556	21.679501203237802
26	18.88339531831109	25.255305184861083	32.600743819733104	23.260555677094725
27	22.887551957996063	25.775978998031064	29.96149639028659	21.37497265368628
28	18.18136075257055	26.68300153139357	33.6751257930431	21.46051192299278
29	21.687376941588273	25.85976810325968	30.986873769415883	21.46598118573616
30	19.49398381098228	30.11922992780573	30.817545394880767	19.56924086633122
31	21.375410194705754	25.16385911179173	28.57755414570116	24.883176547801355
32	24.78276088383286	28.62393349376504	26.778385473638156	19.814920148763946
33	22.117698534237583	26.751476700940714	26.884489170859766	24.246335593961934
34	21.53664406038066	26.744257274119448	27.78954276963465	23.92955589586524
35	21.368409538394225	28.5405819295559	26.37475388317655	23.71625464887333
36	21.557208488295778	29.662218332968713	27.883395318311095	20.897177860424414
37	22.021658280463793	27.45219864362284	29.230584117260992	21.295558958652375
38	20.732881207613215	28.473200612557427	27.633778166703127	23.16014001312623
39	24.516079632465544	24.933493765040474	26.938744257274124	23.611682345219865
40	20.09188361408882	28.56530299715598	29.13935681470138	22.203456574053817
41	21.08444541675782	26.81929555895865	27.21133231240429	24.88492671187924
42	21.070662874644498	27.659374316342156	29.757821045723038	21.51214176329031
43	23.817107853861298	25.695252679938747	27.43032159264931	23.057317873550645
44	22.57930430977904	24.493108728943337	29.619776854080072	23.30781010719755
45	21.001093852548678	23.714504484795448	29.03172172391162	26.252679938744254
46	24.090570991030408	27.060599431196675	27.339969372128635	21.50886020564428
47	19.37978560490046	26.37737912929337	32.797637278494854	21.44519798731131
48	21.36819076788449	25.993436884707943	28.35331437322249	24.28505797418508
49	20.8208269525268	23.903522205206738	32.42988405162984	22.845766790636624
50	21.458980529424636	22.925618026690003	30.646904397287244	24.968497046598117
51	20.80179391817983	23.46707503828484	28.432071756727197	27.299059286808138
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	567.0
2	894.0
3	5587.0
4	10280.0
5	6553.5
6	2827.0
7	2755.5
8	2684.0
9	2748.0
10	2812.0
11	2699.0
12	2586.0
13	2504.5
14	2423.0
15	2306.5
16	2190.0
17	2134.0
18	2078.0
19	1974.5
20	1871.0
21	1810.0
22	1749.0
23	1767.0
24	1785.0
25	2205.5
26	3045.5
27	3465.0
28	4152.5
29	4840.0
30	5142.0
31	5444.0
32	6360.5
33	7277.0
34	8485.0
35	9693.0
36	10468.0
37	11243.0
38	12716.0
39	14189.0
40	16112.0
41	18035.0
42	20637.0
43	23239.0
44	40244.5
45	57250.0
46	49355.0
47	41460.0
48	42271.5
49	43083.0
50	37937.5
51	32792.0
52	30631.0
53	28470.0
54	25489.5
55	22509.0
56	21498.5
57	20488.0
58	19518.5
59	18549.0
60	17542.0
61	16535.0
62	14786.5
63	13038.0
64	11502.5
65	9967.0
66	8112.0
67	6257.0
68	5194.0
69	4131.0
70	3486.0
71	2841.0
72	2459.5
73	2078.0
74	1707.5
75	1093.5
76	850.0
77	622.0
78	394.0
79	374.0
80	354.0
81	247.5
82	141.0
83	88.5
84	36.0
85	34.5
86	33.0
87	18.0
88	3.0
89	13.0
90	23.0
91	13.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	457100.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.172689981046304
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59771325250394	25.886295515442647
2	8.864912364423619	6.2360562860603235
3	3.4489763223057595	3.639293248092892
4	2.0802057895151034	2.926657333255696
5	1.4943097245831565	2.6279446339213033
6	1.207148075265697	2.547518700752226
7	1.0773395302088127	2.652505051025245
8	0.8895437057999537	2.5030115990954265
9	0.8239282468461445	2.6081795513651795
>10	6.421021206283505	36.417183570944694
>50	0.05619184476178529	1.4210447830586665
>100	0.030593337703638663	2.1343122000536514
>500	0.00187306149205951	0.4284435746789458
>1k	0.00374612298411902	1.9309607135581601
>5k	0.0024974153227460133	6.040593238694907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	7857	1.7188798949901556	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	6897	1.508860205644279	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6685	1.4624808575803983	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	6068	1.3274994530737256	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	2213	0.4841391380441916	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	1600	0.3500328155764603	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAGT	1466	0.32071756727193174	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTA	1243	0.27193174360096256	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	1183	0.25880551301684535	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	1088	0.23802231459199302	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	840	0.18376722817764166	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	597	0.13060599431196673	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	514	0.11244804200393786	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1349814045066725	0.0
2	0.0	0.0	0.0	0.8987092539925619	0.0
3	0.0	0.0	0.0	1.1739225552395538	0.0
4	0.0	0.0	0.0	1.796105884926712	0.0
5	0.0	0.0	0.0	4.090570991030409	0.0
6	0.0	0.0	0.0	4.82126449354627	0.0
7	0.0	0.0	0.0	5.770509735287683	0.0
8	0.0	0.0	0.0	6.922336469043973	0.0
9	0.0	0.0	0.0	7.332312404287902	0.0
10	2.187705097352877E-4	0.0	0.0	9.984467293808795	0.0
11	2.187705097352877E-4	0.0	0.0	11.237366003062787	0.0
12	2.187705097352877E-4	0.0	0.0	13.707285057974184	0.0
13	2.187705097352877E-4	0.0	0.0	14.164077882301466	0.0
14	2.187705097352877E-4	0.0	0.0	14.380004375410195	0.0
15	2.187705097352877E-4	0.0	0.0	14.939838109822796	0.0
16	2.187705097352877E-4	0.0	0.0	15.622183329687159	0.0
17	2.187705097352877E-4	0.0	0.0	16.37497265368628	0.0
18	2.187705097352877E-4	0.0	0.0	17.166703128418288	0.0
19	2.187705097352877E-4	0.0	0.0	17.95405819295559	0.0
20	4.375410194705754E-4	0.0	0.0	18.47407569459637	0.0
21	4.375410194705754E-4	0.0	0.0	19.04134762633997	0.0
22	4.375410194705754E-4	0.0	0.0	19.66900021877051	0.0
23	4.375410194705754E-4	0.0	0.0	20.25618026690002	0.0
24	4.375410194705754E-4	0.0	0.0	20.690658499234303	0.0
25	4.375410194705754E-4	0.0	0.0	21.105666156202144	0.0
26	4.375410194705754E-4	0.0	0.0	21.489608400787574	0.0
27	4.375410194705754E-4	0.0	0.0	21.906803762852768	0.0
28	4.375410194705754E-4	0.0	0.0	22.304309779041784	0.0
29	4.375410194705754E-4	0.0	0.0	22.686939400568804	0.0
30	4.375410194705754E-4	0.0	0.0	23.133887551957997	0.0
31	4.375410194705754E-4	0.0	0.0	23.551957996062132	0.0
32	4.375410194705754E-4	0.0	0.0	23.963027783854738	0.0
33	4.375410194705754E-4	0.0	0.0	24.367096915335814	0.0
34	4.375410194705754E-4	0.0	0.0	24.77947932618683	0.0
35	6.563115292058631E-4	0.0	0.0	25.2089258367972	0.0
36	6.563115292058631E-4	0.0	0.0	25.613870050317217	0.0
37	6.563115292058631E-4	0.0	0.0	26.01750164077882	0.0
38	6.563115292058631E-4	0.0	0.0	26.408007000656312	0.0
39	6.563115292058631E-4	0.0	0.0	26.785823670969155	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGCG	55	1.8189894E-12	45.0	36
CGTATGG	20	7.028824E-4	45.0	2
CGACGGT	25	3.887106E-5	45.0	28
CCGCTCG	25	3.887106E-5	45.0	19
GGTATCG	20	7.028824E-4	45.0	1
ACAGACG	20	7.028824E-4	45.0	43
TATACGG	25	3.887106E-5	45.0	2
ACGGTCG	20	7.028824E-4	45.0	40
ACCGAAG	30	2.162622E-6	44.999996	17
CTACGAA	30	2.162622E-6	44.999996	11
CGAATAT	30	2.162622E-6	44.999996	14
CGTAAGC	30	2.162622E-6	44.999996	43
GATTGCG	30	2.162622E-6	44.999996	1
TATAGCG	30	2.162622E-6	44.999996	1
TAGTATG	30	2.162622E-6	44.999996	1
CGTTTTT	3120	0.0	43.701923	1
CGATGAA	720	0.0	41.875	19
CAGTCGA	65	0.0	41.53846	41
CGGCGAT	60	3.6379788E-12	41.249996	31
CCGATGA	705	0.0	40.851063	18
>>END_MODULE