FastQCFastQC Report
Sat 18 Jun 2016
SRR3551483_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551483_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721454
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129091.7893032681224306No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTC75421.045388895203298No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCG60490.8384456943893859No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGC45930.6366310256786989No Hit
GCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC25470.3530370612679395No Hit
GAACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCT19340.26806975912532194No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT14080.19516143787407098No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCC12190.16896434145489525No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTC10740.14886603996928427No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT9400.13029243721706443No Hit
CTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGCT8960.12419364228349972Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTTCTGC8880.12308477047739702No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCCAGGTT8630.11961954608332617No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTA8370.11601571271349248No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCTT8070.11185744344060745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG700.045.040
TCTCGAG207.0315396E-445.01
CGTGTAC253.8893573E-545.018
TATAGCG207.0315396E-445.01
CGGTCTA253.8893573E-545.031
CGGTATT207.0315396E-445.043
CGATCGA900.045.041
CGTTTTT53000.043.641511
TAAACGG451.9266736E-840.02
GCGATAA451.9266736E-840.09
TTGGACG403.4558434E-739.3751
CGTAAGG403.4558434E-739.3752
ATAACGC403.4558434E-739.37511
CAACGAT356.2450563E-638.57142638
ACGTAGG700.038.5714262
TATACGG356.2450563E-638.5714262
TAATGCG356.2450563E-638.5714261
CCGATGA6550.037.7862618
GCTACGA900.037.510
CAAACGA301.1395008E-437.49999615