FastQCFastQC Report
Sat 18 Jun 2016
SRR3551479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514623
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.658151695513026No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC25820.5017265065883181No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC11680.22696226169448314No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT11310.21977253251409282Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8300.16128311404659332No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC8280.1608944800368425No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC8070.1568138229344588No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC7980.1550649698905801No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC7800.15156726380282265No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG7640.14845819172481603No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC5890.11445271587161865No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5600.10881752273023165No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA5590.10862320572535622No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG253.8877915E-545.0000041
CAACGCT207.029651E-445.024
GACGTAT207.029651E-445.09
TAGCGAT207.029651E-445.01
ACTAGTC207.029651E-445.043
GTTAACG351.2101737E-745.01
CGGTCTA453.8380676E-1045.031
TTCGCAC207.029651E-445.011
TAGTACG207.029651E-445.01
GTACGAG453.8380676E-1045.01
AAATGCG207.029651E-445.01
TCATCGA302.1631568E-644.99999616
TATAGCG302.1631568E-644.9999961
TCGACAA302.1631568E-644.99999619
CACAACG700.041.78571312
CGTGTAC650.041.53846418
CGAATAT1650.040.9090914
GACCTAT1550.040.64516433
CGTTTTT16000.040.359381
TCGGCGT1400.040.178574