##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514623
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24645614362358	33.0	31.0	34.0	30.0	34.0
2	32.31785404072496	34.0	31.0	34.0	30.0	34.0
3	32.32665854421586	34.0	31.0	34.0	30.0	34.0
4	35.891281578942255	37.0	35.0	37.0	35.0	37.0
5	35.96683397360786	37.0	35.0	37.0	35.0	37.0
6	35.95965590344777	37.0	35.0	37.0	35.0	37.0
7	36.14204184422383	37.0	35.0	37.0	35.0	37.0
8	35.94573503321849	37.0	35.0	37.0	35.0	37.0
9	37.85474609568558	39.0	38.0	39.0	35.0	39.0
10	37.51833089465492	39.0	37.0	39.0	35.0	39.0
11	37.53386653919471	39.0	37.0	39.0	35.0	39.0
12	37.484292384910894	39.0	37.0	39.0	35.0	39.0
13	37.46627531221885	39.0	37.0	39.0	35.0	39.0
14	38.68437477532096	40.0	38.0	41.0	35.0	41.0
15	38.75803646552914	40.0	38.0	41.0	35.0	41.0
16	38.75673065525637	40.0	38.0	41.0	35.0	41.0
17	38.72975362546952	40.0	38.0	41.0	35.0	41.0
18	38.64304743472406	40.0	38.0	41.0	35.0	41.0
19	38.56589969744842	40.0	38.0	41.0	35.0	41.0
20	38.47158793913214	40.0	37.0	41.0	34.0	41.0
21	38.35578666324669	40.0	37.0	41.0	34.0	41.0
22	38.38139570131922	40.0	37.0	41.0	34.0	41.0
23	38.35153695035006	40.0	37.0	41.0	34.0	41.0
24	38.27766151143653	40.0	37.0	41.0	34.0	41.0
25	38.095938191647086	40.0	37.0	41.0	34.0	41.0
26	38.12940735256683	40.0	37.0	41.0	34.0	41.0
27	38.1574570122206	40.0	37.0	41.0	34.0	41.0
28	38.092259770744796	40.0	37.0	41.0	34.0	41.0
29	38.031156788561724	40.0	36.0	41.0	34.0	41.0
30	37.88556088631872	40.0	36.0	41.0	34.0	41.0
31	37.87237453436788	40.0	36.0	41.0	34.0	41.0
32	37.74618895774188	40.0	36.0	41.0	33.0	41.0
33	37.665092698927175	40.0	36.0	41.0	33.0	41.0
34	37.566004628631056	40.0	36.0	41.0	33.0	41.0
35	37.46596440501105	40.0	36.0	41.0	33.0	41.0
36	37.36731160480585	40.0	36.0	41.0	33.0	41.0
37	37.32796435448863	40.0	36.0	41.0	33.0	41.0
38	37.22474899100895	40.0	35.0	41.0	33.0	41.0
39	37.18154066180485	40.0	35.0	41.0	33.0	41.0
40	37.10230595989685	40.0	35.0	41.0	32.0	41.0
41	37.00716446796976	39.0	35.0	41.0	32.0	41.0
42	36.90120729155129	39.0	35.0	41.0	32.0	41.0
43	36.78590152402827	39.0	35.0	41.0	31.0	41.0
44	36.82451425606706	39.0	35.0	41.0	32.0	41.0
45	36.81173596982646	39.0	35.0	41.0	32.0	41.0
46	36.74331500923977	39.0	35.0	41.0	32.0	41.0
47	36.69761164969307	39.0	35.0	41.0	32.0	41.0
48	36.659385608493984	39.0	35.0	41.0	32.0	41.0
49	36.618889944677946	39.0	35.0	41.0	31.0	41.0
50	36.53676963524755	39.0	35.0	41.0	31.0	41.0
51	35.69869593858028	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	4.0
11	11.0
12	8.0
13	16.0
14	23.0
15	17.0
16	39.0
17	50.0
18	122.0
19	202.0
20	307.0
21	573.0
22	808.0
23	1196.0
24	1808.0
25	2731.0
26	3779.0
27	4819.0
28	4956.0
29	5452.0
30	6166.0
31	7656.0
32	9771.0
33	13740.0
34	26055.0
35	38615.0
36	36731.0
37	53834.0
38	98738.0
39	196182.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2279552215894	20.173214178145944	23.779932105638498	12.818898494626163
2	34.44035731010857	23.96239577321651	24.16157070321381	17.435676213461115
3	33.04846460418598	22.88704546823597	26.759394741393216	17.305095186184836
4	29.422120659201006	25.149478356000408	26.2489239695855	19.179477015213077
5	25.085353744391526	29.003950464709117	24.879377719223587	21.03131807167577
6	25.7660462124701	34.00800197426077	24.9093025379744	15.316649275294731
7	76.02827701054947	6.598616851559297	11.737135728484734	5.6359704094064975
8	74.88569302188203	7.353538415500278	11.298562248480927	6.462206314136757
9	68.55037571192892	8.478828190733799	14.532774477627312	8.438021619709962
10	35.96030492224405	25.53830668275611	21.944607994590214	16.55678040040962
11	28.970916573880295	24.547872908906132	25.40597680243596	21.075233714777614
12	25.940542882848224	21.218251030365916	31.86915470159709	20.97205138518877
13	24.224140778783692	24.204514761291275	31.691937593150715	19.879406866774318
14	20.509965547595037	27.290074481707972	29.828631833400372	22.371328137296622
15	19.70763063446445	25.04338127133844	34.17161689236587	21.077371201831244
16	21.67314713877926	23.689963332381183	32.08581816203318	22.55107136680638
17	21.821022379489452	24.059165641644466	28.825567454233486	25.294244524632596
18	21.817718990406572	24.345200272821074	30.759410286753603	23.077670450018754
19	23.416364989516598	25.13976250575664	28.908540815315288	22.53533168941147
20	24.42389865979562	24.871022088013945	29.549009663384652	21.15606958880579
21	23.70434279074196	25.76371440841159	29.495766804048788	21.036175996797656
22	21.615434988331263	24.623462223802665	27.65228137879574	26.10882140907033
23	21.367486490110235	25.940931516857972	29.03445823447456	23.657123758557237
24	22.368219065218618	24.46023593970732	29.49052024491715	23.68102475015691
25	21.072901910719107	26.83459542228	27.887599271699866	24.204903395301024
26	20.455362469225044	27.024637453048157	28.215995009939316	24.304005067787486
27	22.765014389174212	25.3432124098612	29.447964820849435	22.44380838011515
28	20.88130534391195	25.584165495906714	30.056177046109482	23.478352114071853
29	21.473583574772213	25.306292178934868	28.881142117627856	24.338982128665062
30	22.13931363347538	25.23420057012609	29.112767987439348	23.513717808959182
31	23.856298688554535	26.085309051480404	26.423809273973376	23.63458298599169
32	22.695643218433688	26.751622061198198	26.454705677748564	24.098029042619547
33	22.546602075694246	24.352972953016092	27.707661725185233	25.392763246104426
34	21.96870330319477	24.42117822172736	28.54497369919339	25.065144775884484
35	22.236472135913086	23.469996482862214	28.099016173004316	26.194515208220388
36	23.28869871731345	25.21690635669218	28.53195445986674	22.96244046612763
37	22.334019272360546	25.25499248964776	29.898780272160398	22.512207965831298
38	22.259012908478635	25.494585356659144	27.03746237536993	25.208939359492287
39	22.06819360969098	23.31065653886437	29.459235207132213	25.16191464431244
40	22.96302341714226	23.232735419909332	30.688095945964328	23.116145216984084
41	21.172975168229947	24.210927222452163	29.712041630475124	24.904055978842766
42	22.08160148302738	24.942336428803223	29.83038068644425	23.145681401725145
43	22.96963019530802	23.31454287896188	29.365768727787138	24.35005819794296
44	22.095786624383287	23.42938422884325	28.808273240799576	25.666555905973887
45	22.13076368526086	22.920273676069666	27.996028160420344	26.952934478249123
46	23.094576029442912	24.260089424685642	28.465303727194467	24.180030818676972
47	21.12400728300134	23.63866364309407	31.596139309747134	23.641189764157453
48	21.233602073751076	23.356709669019846	29.90383251428716	25.505855742941918
49	21.75417732981231	22.39134278879879	32.066192144540764	23.78828773684814
50	21.031123754670894	22.09345482032478	30.790695324538547	26.084726100465776
51	21.03947938588054	22.43933908900302	28.390880314327188	28.130301210789256
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	286.0
1	576.5
2	867.0
3	3028.5
4	5190.0
5	3392.0
6	1594.0
7	1623.0
8	1652.0
9	1642.0
10	1632.0
11	1663.5
12	1695.0
13	1644.0
14	1593.0
15	1603.0
16	1613.0
17	1588.0
18	1563.0
19	1485.0
20	1407.0
21	1515.5
22	1624.0
23	1831.5
24	2039.0
25	2455.0
26	3699.5
27	4528.0
28	5241.5
29	5955.0
30	6172.5
31	6390.0
32	7697.5
33	9005.0
34	10575.5
35	12146.0
36	12575.5
37	13005.0
38	13938.0
39	14871.0
40	17421.5
41	19972.0
42	22626.0
43	25280.0
44	29003.0
45	32726.0
46	38776.0
47	44826.0
48	49180.5
49	53535.0
50	53174.0
51	52813.0
52	45269.0
53	37725.0
54	33532.5
55	29340.0
56	26834.5
57	24329.0
58	23269.0
59	22209.0
60	20840.0
61	19471.0
62	17863.5
63	16256.0
64	14550.0
65	12844.0
66	10922.5
67	9001.0
68	8348.5
69	7696.0
70	6504.5
71	5313.0
72	4751.5
73	4190.0
74	3188.0
75	1879.5
76	1573.0
77	1212.0
78	851.0
79	697.5
80	544.0
81	436.0
82	328.0
83	197.0
84	66.0
85	38.5
86	11.0
87	9.0
88	7.0
89	4.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	514623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.486206648734132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50129556109115	22.827907743374666
2	10.323126119955687	6.500721645477395
3	3.8005342024774516	3.58993215824361
4	1.9359676143154383	2.438251054783709
5	1.1886316207627068	1.8712750420277184
6	0.8922349642603103	1.6855856678355625
7	0.7534862146204809	1.660709586235903
8	0.6429043036562949	1.61940942082261
9	0.5425891014900307	1.5375665317379474
>10	7.2357328543017605	47.405431520711424
>50	0.11344605432963739	2.44398008636302
>100	0.0619923794151024	3.533728574675834
>500	0.005579314147359216	1.271666022095286
>1k	0.0024796951766040956	1.6138349456153223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3387	0.658151695513026	No Hit
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2582	0.5017265065883181	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1168	0.22696226169448314	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	1131	0.21977253251409282	Illumina Single End Adapter 2 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	830	0.16128311404659332	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	828	0.1608944800368425	No Hit
GTGAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	807	0.1568138229344588	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	798	0.1550649698905801	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	780	0.15156726380282265	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	764	0.14845819172481603	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	589	0.11445271587161865	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	560	0.10881752273023165	No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	559	0.10862320572535622	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22715657869935857	0.0
2	0.0	0.0	0.0	1.2352731999930047	0.0
3	0.0	0.0	0.0	1.6643251467579179	0.0
4	0.0	0.0	0.0	2.2878884154031205	0.0
5	0.0	0.0	0.0	4.002347349418895	0.0
6	0.0	0.0	0.0	4.900091911943306	0.0
7	0.0	0.0	0.0	5.889942734778663	0.0
8	0.0	0.0	0.0	7.2942717290132775	0.0
9	0.0	0.0	0.0	7.858179677161728	0.0
10	0.0	0.0	0.0	9.210626031094607	0.0
11	0.0	0.0	0.0	10.773712018312434	0.0
12	0.0	0.0	0.0	12.212046488400246	0.0
13	0.0	0.0	0.0	12.707943484842302	0.0
14	0.0	0.0	0.0	12.943844328761054	0.0
15	0.0	0.0	0.0	13.368426984413833	0.0
16	0.0	0.0	0.0	14.200881033300105	0.0
17	0.0	0.0	0.0	15.140598068877605	0.0
18	0.0	0.0	0.0	16.230716466228674	0.0
19	0.0	0.0	0.0	16.831156011293704	0.0
20	0.0	0.0	0.0	17.382627671130127	0.0
21	0.0	0.0	0.0	18.068566698340337	0.0
22	0.0	0.0	0.0	18.77821240014535	0.0
23	0.0	0.0	0.0	19.45890486822392	0.0
24	0.0	0.0	0.0	20.028836643523512	0.0
25	0.0	0.0	0.0	20.54163921938973	0.0
26	0.0	0.0	0.0	21.02451697650513	0.0
27	0.0	0.0	0.0	21.506034514586407	0.0
28	0.0	0.0	0.0	21.990272490735936	0.0
29	0.0	0.0	0.0	22.479174075002476	0.0
30	0.0	0.0	0.0	23.00072091608809	0.0
31	0.0	0.0	0.0	23.496617912530144	0.0
32	0.0	0.0	0.0	23.986879715830813	0.0
33	0.0	0.0	0.0	24.448188285405045	0.0
34	0.0	0.0	0.0	24.923487679330307	0.0
35	0.0	0.0	0.0	25.43473571915752	0.0
36	1.9431700487541366E-4	0.0	0.0	25.89779314177563	0.0
37	1.9431700487541366E-4	0.0	0.0	26.36551417251075	0.0
38	1.9431700487541366E-4	0.0	0.0	26.811471698699826	0.0
39	1.9431700487541366E-4	0.0	0.0	27.250628129718258	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	25	3.8877915E-5	45.000004	1
CAACGCT	20	7.029651E-4	45.0	24
GACGTAT	20	7.029651E-4	45.0	9
TAGCGAT	20	7.029651E-4	45.0	1
ACTAGTC	20	7.029651E-4	45.0	43
GTTAACG	35	1.2101737E-7	45.0	1
CGGTCTA	45	3.8380676E-10	45.0	31
TTCGCAC	20	7.029651E-4	45.0	11
TAGTACG	20	7.029651E-4	45.0	1
GTACGAG	45	3.8380676E-10	45.0	1
AAATGCG	20	7.029651E-4	45.0	1
TCATCGA	30	2.1631568E-6	44.999996	16
TATAGCG	30	2.1631568E-6	44.999996	1
TCGACAA	30	2.1631568E-6	44.999996	19
CACAACG	70	0.0	41.785713	12
CGTGTAC	65	0.0	41.538464	18
CGAATAT	165	0.0	40.90909	14
GACCTAT	155	0.0	40.645164	33
CGTTTTT	1600	0.0	40.35938	1
TCGGCGT	140	0.0	40.17857	4
>>END_MODULE