FastQCFastQC Report
Sat 18 Jun 2016
SRR3551478_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551478_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591394
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98041.6577780633553942No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC13940.23571426155828434No Hit
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC11840.20020493951578813Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT9770.16520289350247044TruSeq Adapter, Index 23 (96% over 26bp)
CGTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTT9200.15556464894807862No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC9140.15455009688972157Illumina PCR Primer Index 7 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTC7610.1286790194016172TruSeq Adapter, Index 23 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG7040.11904077484722536TruSeq Adapter, Index 23 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC5980.10111702181625108Illumina PCR Primer Index 7 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG253.8884995E-545.0000041
TCGATTA207.030505E-445.041
AATTACG207.030505E-445.01
ACGTACG207.030505E-445.01
TGCAACG302.163708E-644.9999961
CGTTTTT48250.043.3678741
AGTACGG700.041.7857132
TAAGCGG501.0804797E-940.5000042
ATGACGG800.039.3752
CGTTTCT800.039.3751
CTAACGG700.038.5714262
ACTAACG356.2431463E-638.5714261
CGGTCTA356.2431463E-638.57142631
CGATAAC356.2431463E-638.57142610
AACGAGC950.037.89473715
TCGTTTG301.1392519E-437.4999961
CCAACGG301.1392519E-437.4999962
CGTTTAC301.1392519E-437.49999633
GTCTTAC1550.036.2903231
GTTTTTT60200.036.0299032
Produced by FastQC (version 0.11.5)