SRR3551477_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3551477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394977
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	10806	2.735855505510447	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	9470	2.3976079619825965	RNA PCR Primer, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	9160	2.3191223792777804	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3678	0.9311934618977814	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3536	0.8952420014329948	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	2289	0.5795274155203974	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT	1923	0.4868637920689053	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	1612	0.40812503006504175	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	1508	0.3817943829640713	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	1384	0.350400149882145	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1382	0.3498937912840494	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1368	0.34634928109738033	RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	735	0.1860867848001276	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	635	0.16076885489534834	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	534	0.13519774569152126	RNA PCR Primer, Index 16 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATG	489	0.12380467723437062	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	423	0.10709484349721629	Illumina PCR Primer Index 7 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2092278E-7	45.000004	2
CGAACCC	45	3.8380676E-10	45.0	34
CATGCGG	40	6.7957444E-9	45.0	2
TTTCGGC	25	3.88614E-5	45.0	13
TTATGCG	20	7.027657E-4	45.0	1
GCCATGC	20	7.027657E-4	45.0	27
GCTCTAC	20	7.027657E-4	45.0	22
CTACGGA	25	3.88614E-5	45.0	35
CGCTACG	25	3.88614E-5	45.0	33
CGGGCTA	20	7.027657E-4	45.0	6
TCATGCG	20	7.027657E-4	45.0	1
AACGAGC	55	1.8189894E-12	45.0	15
CTACCTT	20	7.027657E-4	45.0	17
ATACGGT	20	7.027657E-4	45.0	6
GACGAAT	20	7.027657E-4	45.0	20
TCGACAG	20	7.027657E-4	45.0	1
ATCGCCA	25	3.88614E-5	45.0	24
TGGTCGC	20	7.027657E-4	45.0	44
TGCGTAG	25	3.88614E-5	45.0	1
CATCGTT	30	2.1618707E-6	44.999996	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content