##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394977
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08655440696547	33.0	31.0	34.0	30.0	34.0
2	32.17395696458275	33.0	31.0	34.0	30.0	34.0
3	32.20069523035519	34.0	31.0	34.0	30.0	34.0
4	35.81435627897321	37.0	35.0	37.0	35.0	37.0
5	35.843411135331934	37.0	35.0	37.0	35.0	37.0
6	35.80827491221008	37.0	35.0	37.0	35.0	37.0
7	35.977413874731944	37.0	35.0	37.0	35.0	37.0
8	35.65052142276639	37.0	35.0	37.0	33.0	37.0
9	37.59623218567157	39.0	37.0	39.0	35.0	39.0
10	37.349384900892964	39.0	37.0	39.0	34.0	39.0
11	37.37227484131987	39.0	37.0	39.0	34.0	39.0
12	37.28940925674153	39.0	37.0	39.0	34.0	39.0
13	37.195700002784974	39.0	37.0	39.0	34.0	39.0
14	38.440886431361825	40.0	38.0	41.0	34.0	41.0
15	38.36677578694456	40.0	38.0	41.0	34.0	41.0
16	38.51378941052264	40.0	38.0	41.0	34.0	41.0
17	38.35989437359644	40.0	38.0	41.0	34.0	41.0
18	38.292923385412315	40.0	38.0	41.0	34.0	41.0
19	38.23522382315932	40.0	37.0	41.0	34.0	41.0
20	37.95268838438694	40.0	36.0	41.0	33.0	41.0
21	38.03487291665084	40.0	36.0	41.0	34.0	41.0
22	37.99767328224175	40.0	36.0	41.0	34.0	41.0
23	37.899325783526635	40.0	36.0	41.0	33.0	41.0
24	37.88536800877013	40.0	36.0	41.0	33.0	41.0
25	37.82010851264757	40.0	36.0	41.0	33.0	41.0
26	37.78788891505075	40.0	36.0	41.0	33.0	41.0
27	37.70823617577732	40.0	36.0	41.0	33.0	41.0
28	37.588236783407645	40.0	36.0	41.0	33.0	41.0
29	37.51133863490786	40.0	36.0	41.0	33.0	41.0
30	37.41420892862116	40.0	36.0	41.0	33.0	41.0
31	37.33115345956853	40.0	36.0	41.0	32.0	41.0
32	37.06790015621163	40.0	35.0	41.0	32.0	41.0
33	36.881775394516644	39.0	35.0	41.0	31.0	41.0
34	36.66360826073417	39.0	35.0	41.0	30.0	41.0
35	36.6718441833322	40.0	35.0	41.0	30.0	41.0
36	36.59274337493069	39.0	35.0	41.0	30.0	41.0
37	36.510989247475166	39.0	35.0	41.0	30.0	41.0
38	36.46383713482051	39.0	35.0	41.0	30.0	41.0
39	36.40831238274634	39.0	35.0	41.0	30.0	41.0
40	36.38451859222182	39.0	35.0	41.0	30.0	41.0
41	36.19475564399952	39.0	35.0	41.0	29.0	41.0
42	36.16988330966107	39.0	35.0	41.0	29.0	41.0
43	36.04326834220727	39.0	35.0	41.0	29.0	41.0
44	36.16167017319996	39.0	35.0	41.0	30.0	41.0
45	36.15285193821413	39.0	35.0	41.0	30.0	41.0
46	36.0773462758591	39.0	35.0	41.0	30.0	41.0
47	35.906265428113535	39.0	35.0	41.0	29.0	41.0
48	35.93472024953352	39.0	35.0	41.0	29.0	41.0
49	36.00333690316145	39.0	35.0	41.0	29.0	41.0
50	35.870607655635645	39.0	35.0	41.0	29.0	41.0
51	35.07489803203731	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	3.0
11	6.0
12	5.0
13	8.0
14	9.0
15	16.0
16	21.0
17	48.0
18	86.0
19	236.0
20	540.0
21	1047.0
22	1659.0
23	2269.0
24	2881.0
25	3580.0
26	4378.0
27	4636.0
28	4603.0
29	4702.0
30	5447.0
31	7181.0
32	9195.0
33	12128.0
34	20210.0
35	29180.0
36	28636.0
37	41774.0
38	76205.0
39	134131.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.19678867377088	17.340756550381414	19.915590021697465	13.546864754150242
2	30.135172427761614	33.708798233821206	20.236115014291972	15.919914324125203
3	27.79959339404573	32.73608336687959	24.75663139879031	14.70769184028437
4	25.319702159872598	21.154649511237363	36.92974527630723	16.59590305258281
5	31.801851753393233	24.20368780966993	24.058869250614592	19.93559118632224
6	21.5177086260719	39.310137045954576	25.39768138397932	13.774472943994207
7	66.79756036427437	5.836289201649716	22.11040136514278	5.255749068933127
8	63.92954526466098	14.915552044802608	14.208422262562124	6.946480427974287
9	59.45839884347697	6.9849636814295515	14.914539327606418	18.64209814748707
10	37.972084450486996	21.185537385721194	23.591753443871415	17.250624719920403
11	30.90888836565167	21.463021897477574	26.346344217511398	21.281745519359355
12	24.333062431483352	18.005605389680916	32.377328300128866	25.284003878706862
13	24.11355597920892	20.9323580866734	36.96265858518344	17.99142734893424
14	19.463918152196204	29.591343293406958	28.420389035310915	22.52434951908592
15	16.586029059919944	22.67929525010317	38.94935654481147	21.785319145165417
16	17.782048068621716	24.29179420573856	29.279426396980078	28.64673132865964
17	18.12029561214957	25.65288611741949	31.646399663777892	24.580418606653044
18	18.744888942900474	23.721887603581983	32.133516635145845	25.399706818371705
19	19.62949741377346	25.42046752089362	28.619894322960576	26.330140742372343
20	22.963362423634795	24.551556166561596	33.47941778888391	19.005663620919698
21	20.996665628631543	30.31948695746841	29.11764482488854	19.56620258901151
22	18.54563683454986	22.47624545226684	31.618296761583586	27.359820951599712
23	20.55182960020457	28.030492914777316	29.57362074247361	21.844056742544502
24	22.137238370841846	23.933292318286888	27.831493985725754	26.097975325145516
25	18.03396147117427	32.56746595371376	26.065821554166448	23.332751020945526
26	18.56867615076321	24.61231919833307	31.098266481339422	25.720738169564306
27	25.525030571400357	24.803722748413197	26.522303830349614	23.148942849836825
28	17.57317514690729	25.702002901434767	33.24877144745137	23.476050504206576
29	21.941530772677904	24.57054461399018	28.023150715104926	25.464773898226984
30	23.161348635490167	27.45678862313502	27.621861526114177	21.760001215260637
31	21.80734574418257	25.19083389665727	26.1911453071951	26.81067505196505
32	23.931266883894505	26.874982593923193	23.020834124518643	26.17291639766366
33	21.265795223519344	22.49852523058305	23.77733387007345	32.45834567582416
34	19.75254255311069	28.87737767009218	25.262483638287797	26.107596138509333
35	22.693979649447943	22.713980814072716	23.439592685143694	31.152446851335647
36	20.041166954025172	28.248480291257465	25.57338781751849	26.136964937198876
37	21.111102671801145	23.836324646751585	28.56090354628244	26.49166913516483
38	18.401577813391665	23.23679606660641	25.64427802125187	32.71734809875005
39	21.43947622266613	25.66833005466141	24.843978256961798	28.048215465710662
40	19.851282479739325	22.082298462948476	27.319818622350162	30.746600434962033
41	18.194223967471522	27.1722150910053	23.876326976001135	30.75723396552204
42	19.851535659038376	21.616954911298635	29.960225532119594	28.571283897543402
43	22.17470890710092	21.840259053058787	25.755930092131944	30.229101947708347
44	21.96684870258268	21.338710861645108	28.958648225086524	27.735792210685684
45	20.227253738825297	20.274598267747233	28.140625909863104	31.35752208356436
46	25.586553141068975	24.783468404489376	25.194378406843942	24.43560004759771
47	17.490638695417708	23.064380963954864	34.900766373738215	24.54421396688921
48	20.820959195092374	23.963927013471668	26.813966382852673	28.401147408583284
49	20.07889066958329	19.478096192942882	34.010081599688085	26.43293153778574
50	20.671583408654175	19.966225881507025	30.023773536180588	29.338417173658215
51	20.463470024836887	20.024457120288016	26.6731986925821	32.838874162292996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	403.5
2	642.0
3	2991.5
4	5341.0
5	3429.0
6	1517.0
7	1455.5
8	1394.0
9	1352.0
10	1310.0
11	1379.5
12	1449.0
13	1391.0
14	1333.0
15	1277.5
16	1222.0
17	1178.5
18	1135.0
19	1157.5
20	1180.0
21	1221.5
22	1263.0
23	1352.0
24	1441.0
25	1806.0
26	2343.0
27	2515.0
28	2937.0
29	3359.0
30	4093.5
31	4828.0
32	4970.5
33	5113.0
34	5907.0
35	6701.0
36	7312.0
37	7923.0
38	8513.5
39	9104.0
40	10307.5
41	11511.0
42	13231.5
43	14952.0
44	16669.0
45	18386.0
46	20704.0
47	23022.0
48	45679.0
49	68336.0
50	55417.5
51	42499.0
52	41855.0
53	41211.0
54	33966.0
55	26721.0
56	23696.0
57	20671.0
58	18523.5
59	16376.0
60	14948.5
61	13521.0
62	12169.5
63	10818.0
64	9394.5
65	7971.0
66	6689.5
67	5408.0
68	4944.5
69	4481.0
70	3648.5
71	2816.0
72	2452.5
73	2089.0
74	1694.5
75	1067.0
76	834.0
77	699.5
78	565.0
79	403.0
80	241.0
81	168.5
82	96.0
83	61.0
84	26.0
85	21.0
86	16.0
87	9.0
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	394977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.74228485817414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.36966036351313	25.490296402966617
2	9.59876919120286	6.6696634705727105
3	3.617763108328719	3.770680693768496
4	2.1116378896388	2.934525003165795
5	1.4824115632440766	2.575118240363847
6	1.2106280384003583	2.5235990500438694
7	1.0481133888648293	2.5489697743725706
8	0.9173775529176451	2.5497433812767643
9	0.8096412977669769	2.531590973996589
>10	5.71614700753752	31.198401577584395
>50	0.06075527753142829	1.4462638194160617
>100	0.04611545162024075	3.0405363504375655
>500	0.002195973886678131	0.4842071939806896
>1k	0.006587921660034393	4.750520159432395
>5k	0.001463982591118754	4.737804634380382
>10k+	7.31991295559377E-4	2.7480792742412454
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCG	10806	2.735855505510447	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	9470	2.3976079619825965	RNA PCR Primer, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGC	9160	2.3191223792777804	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	3678	0.9311934618977814	Illumina PCR Primer Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3536	0.8952420014329948	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCT	2289	0.5795274155203974	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCCACCT	1923	0.4868637920689053	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTA	1612	0.40812503006504175	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCT	1508	0.3817943829640713	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTC	1384	0.350400149882145	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1382	0.3498937912840494	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	1368	0.34634928109738033	RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCC	735	0.1860867848001276	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	635	0.16076885489534834	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTT	534	0.13519774569152126	RNA PCR Primer, Index 16 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATG	489	0.12380467723437062	No Hit
ACTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	423	0.10709484349721629	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3549573772650053	0.0
2	0.0	0.0	0.0	1.957329160938485	0.0
3	0.0	0.0	0.0	2.4745744688931253	0.0
4	0.0	0.0	0.0	3.445770260040458	0.0
5	0.0	0.0	0.0	7.395620504485072	0.0
6	0.0	0.0	0.0	8.369348088622882	0.0
7	0.0	0.0	0.0	9.844117505576275	0.0
8	0.0	0.0	0.0	11.48598525990121	0.0
9	0.0	0.0	0.0	12.039688386918732	0.0
10	0.0	0.0	0.0	15.94776404702046	0.0
11	0.0	0.0	0.0	17.681282707600694	0.0
12	0.0	0.0	0.0	21.470870455748056	0.0
13	0.0	0.0	0.0	22.176481162194253	0.0
14	0.0	0.0	0.0	22.51624778151639	0.0
15	0.0	0.0	0.0	23.35376490276649	0.0
16	0.0	0.0	0.0	24.21482769882803	0.0
17	0.0	0.0	0.0	25.211594599179193	0.0
18	0.0	0.0	0.0	26.219754567987504	0.0
19	0.0	0.0	0.0	27.35095967613304	0.0
20	0.0	0.0	0.0	28.022644356506834	0.0
21	0.0	0.0	0.0	28.70597528463683	0.0
22	0.0	0.0	0.0	29.406269225803022	0.0
23	0.0	0.0	0.0	30.083017492157772	0.0
24	0.0	0.0	0.0	30.61520037875623	0.0
25	0.0	0.0	0.0	31.10383642591847	0.0
26	0.0	0.0	0.0	31.571711770558792	0.0
27	0.0	0.0	0.0	32.09680563678391	0.0
28	0.0	0.0	0.0	32.54467981679946	0.0
29	0.0	0.0	0.0	32.97685688027404	0.0
30	0.0	0.0	0.0	33.467012003230565	0.0
31	0.0	0.0	0.0	33.91716479693754	0.0
32	0.0	0.0	0.0	34.373393893821664	0.0
33	0.0	0.0	0.0	34.796456502530525	0.0
34	0.0	0.0	0.0	35.20888558067938	0.0
35	0.0	0.0	0.0	35.67473549092732	0.0
36	0.0	0.0	0.0	36.10134260982285	0.0
37	0.0	0.0	0.0	36.51959481184981	0.0
38	0.0	0.0	0.0	36.91860538714913	0.0
39	0.0	0.0	0.0	37.323439086326545	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2092278E-7	45.000004	2
CGAACCC	45	3.8380676E-10	45.0	34
CATGCGG	40	6.7957444E-9	45.0	2
TTTCGGC	25	3.88614E-5	45.0	13
TTATGCG	20	7.027657E-4	45.0	1
GCCATGC	20	7.027657E-4	45.0	27
GCTCTAC	20	7.027657E-4	45.0	22
CTACGGA	25	3.88614E-5	45.0	35
CGCTACG	25	3.88614E-5	45.0	33
CGGGCTA	20	7.027657E-4	45.0	6
TCATGCG	20	7.027657E-4	45.0	1
AACGAGC	55	1.8189894E-12	45.0	15
CTACCTT	20	7.027657E-4	45.0	17
ATACGGT	20	7.027657E-4	45.0	6
GACGAAT	20	7.027657E-4	45.0	20
TCGACAG	20	7.027657E-4	45.0	1
ATCGCCA	25	3.88614E-5	45.0	24
TGGTCGC	20	7.027657E-4	45.0	44
TGCGTAG	25	3.88614E-5	45.0	1
CATCGTT	30	2.1618707E-6	44.999996	36
>>END_MODULE