##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367027
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.212962534091496	33.0	31.0	34.0	30.0	34.0
2	32.30111136237934	34.0	31.0	34.0	30.0	34.0
3	32.31554081852289	34.0	31.0	34.0	30.0	34.0
4	35.88906810670605	37.0	35.0	37.0	35.0	37.0
5	35.96021273639269	37.0	35.0	37.0	35.0	37.0
6	35.9479438842374	37.0	35.0	37.0	35.0	37.0
7	36.133507889065385	37.0	35.0	37.0	35.0	37.0
8	35.92941118773278	37.0	35.0	37.0	35.0	37.0
9	37.828887248077116	39.0	38.0	39.0	35.0	39.0
10	37.52189076008032	39.0	37.0	39.0	35.0	39.0
11	37.53447021608765	39.0	37.0	39.0	35.0	39.0
12	37.474087737414415	39.0	37.0	39.0	35.0	39.0
13	37.440310930803456	39.0	37.0	39.0	35.0	39.0
14	38.682402112106196	40.0	38.0	41.0	35.0	41.0
15	38.73437104082261	40.0	38.0	41.0	35.0	41.0
16	38.7491492451509	40.0	38.0	41.0	35.0	41.0
17	38.70715778403224	40.0	38.0	41.0	35.0	41.0
18	38.6364163944342	40.0	38.0	41.0	35.0	41.0
19	38.55981440057543	40.0	38.0	41.0	35.0	41.0
20	38.48854716410509	40.0	38.0	41.0	34.0	41.0
21	38.38055783361987	40.0	37.0	41.0	34.0	41.0
22	38.42275091478284	40.0	37.0	41.0	34.0	41.0
23	38.37839450503641	40.0	37.0	41.0	34.0	41.0
24	38.292885809490855	40.0	37.0	41.0	34.0	41.0
25	38.106534396652016	40.0	37.0	41.0	34.0	41.0
26	38.145005680780976	40.0	37.0	41.0	34.0	41.0
27	38.1209910987475	40.0	37.0	41.0	34.0	41.0
28	38.05777776566847	40.0	37.0	41.0	34.0	41.0
29	38.03140913338801	40.0	37.0	41.0	34.0	41.0
30	37.85914115310318	40.0	36.0	41.0	33.0	41.0
31	37.82811346304223	40.0	36.0	41.0	33.0	41.0
32	37.72901993586303	40.0	36.0	41.0	33.0	41.0
33	37.630296953630115	40.0	36.0	41.0	33.0	41.0
34	37.520623823315454	40.0	36.0	41.0	33.0	41.0
35	37.41579502325442	40.0	36.0	41.0	33.0	41.0
36	37.32206349941558	40.0	35.0	41.0	33.0	41.0
37	37.278750609628176	40.0	35.0	41.0	33.0	41.0
38	37.17497622790694	40.0	35.0	41.0	32.0	41.0
39	37.157369893768035	40.0	35.0	41.0	32.0	41.0
40	37.05784043135791	39.0	35.0	41.0	32.0	41.0
41	36.952690129064074	39.0	35.0	41.0	32.0	41.0
42	36.82159623133993	39.0	35.0	41.0	31.0	41.0
43	36.72335277786103	39.0	35.0	41.0	31.0	41.0
44	36.75282472406662	39.0	35.0	41.0	31.0	41.0
45	36.72418377939498	39.0	35.0	41.0	31.0	41.0
46	36.62289967768039	39.0	35.0	41.0	31.0	41.0
47	36.524658948796684	39.0	35.0	41.0	31.0	41.0
48	36.45737234590371	39.0	35.0	41.0	31.0	41.0
49	36.381219910251836	39.0	35.0	41.0	31.0	41.0
50	36.24597917864353	38.0	35.0	40.0	31.0	41.0
51	35.37864789238939	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	4.0
11	7.0
12	7.0
13	12.0
14	13.0
15	20.0
16	31.0
17	57.0
18	81.0
19	134.0
20	211.0
21	444.0
22	624.0
23	915.0
24	1411.0
25	1959.0
26	2768.0
27	3285.0
28	3450.0
29	3834.0
30	4571.0
31	5574.0
32	7238.0
33	10052.0
34	18694.0
35	27821.0
36	26527.0
37	39783.0
38	73460.0
39	133878.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56020946687846	20.2178586316538	23.11655545777286	12.105376443694878
2	34.121467902906325	25.076629239810693	23.97425802461399	16.827644832668987
3	32.40660768826272	24.68564982957657	25.959943001468556	16.947799480692154
4	29.07388284785588	25.13983984829454	26.845163979761704	18.941113324087873
5	26.528293558784505	29.0488165720778	24.260340519907256	20.16254934923044
6	25.17335236917175	35.80417789426936	23.96145242720563	15.061017309353264
7	76.72269342582426	6.889138946181071	11.491525146651336	4.896642481343334
8	75.5925313396562	8.050088958033061	10.539279126603764	5.818100575706964
9	68.43229517174485	9.608284949063693	13.214559146874752	8.744860732316697
10	37.73101161494931	25.02486193113858	20.739618611164843	16.504507842747262
11	30.285782789822	24.705539374487437	24.252166734327446	20.756511101363113
12	26.52774863974585	22.26239486468298	30.15799927525768	21.05185722031349
13	24.84067929607359	23.912409713726785	31.444825585038704	19.802085405160927
14	21.24176150528435	27.706680979873415	29.569214253992214	21.48234326085002
15	20.58567898274514	25.37987668482155	33.614965656477594	20.41947867595572
16	22.52749797698807	24.030384685595337	31.308050906336593	22.13406643108
17	22.493168077552877	24.781828039898972	28.462756146005606	24.262247736542545
18	22.796143063044408	24.048367013870912	30.24791091663556	22.907579006449115
19	24.07806510147755	25.530819258528663	28.39191667098061	21.99919896901318
20	24.773381794799835	25.794015154198462	28.501717857269355	20.93088519373234
21	24.078337560996875	26.417674993937773	28.84855882537252	20.655428619692827
22	22.050421358646638	24.62816087099859	27.722756091513705	25.59866167884107
23	21.976857288428373	26.295613129279317	28.53577529718522	23.19175428510709
24	22.903764573178538	25.6141918714427	28.70197560397464	22.78006795140412
25	22.51659959621499	26.80892686369123	27.400164020630633	23.274309519463145
26	22.308712982968558	26.591776626787677	27.72466330814899	23.374847082094778
27	23.930664501521687	26.678963672972287	27.817299544720143	21.573072280785883
28	20.847512580818307	27.043242050312376	29.428080222980867	22.68116514588845
29	23.186305094720552	26.002174226964232	27.560370217994862	23.251150460320357
30	23.682453879414865	27.57971484386707	26.711931274810848	22.025900001907218
31	24.556504017415612	28.131172910984752	24.333359671086868	22.978963400512768
32	23.918131363632646	28.69407427791416	24.749405357099068	22.638389001354124
33	23.859007647938707	28.16114345811071	24.457328752380615	23.522520141569967
34	23.855465674187457	27.019538072130935	25.23901511332954	23.885981140352072
35	23.96281472480226	27.098278873216415	24.926504044661566	24.01240235731976
36	25.079898754042617	30.17380192737864	23.57428745024208	21.172011868336664
37	22.349581910867595	30.438632580164406	25.668956234827412	21.542829274140594
38	22.33241696115	30.375966890719212	23.69880145057448	23.59281469755631
39	24.81179858702493	26.130775120086536	26.107616060943744	22.949810231944788
40	25.06300626384435	25.370068142125785	27.663904835339086	21.90302075869078
41	22.349309451348265	28.15242475349225	25.904361259525864	23.593904535633616
42	21.848256395306066	29.257248104362894	26.629648499974117	22.264847000356923
43	22.910576061161713	26.39478839431432	27.146776667656603	23.54785887686737
44	23.187394932797858	25.943322970789616	26.71002405817556	24.15925803823697
45	23.774545196947365	24.982630705642908	25.58313148623943	25.659692611170293
46	24.267424467409754	26.83780757273988	26.38988412296643	22.504883836883934
47	22.61196042797941	26.78140845223921	27.99140117757004	22.615229942211336
48	21.76243164671809	26.975944549038626	27.290907753380537	23.97071605086274
49	22.01500162113414	26.038411343034706	28.66873554261676	23.277851493214396
50	22.074670255866735	24.56277058636013	28.28674729652042	25.075811861252717
51	22.27574538113	25.024317012099928	26.179545374046054	26.52039223272402
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	211.0
1	534.0
2	857.0
3	1956.0
4	3055.0
5	2039.5
6	1024.0
7	1069.0
8	1114.0
9	1113.0
10	1112.0
11	1133.5
12	1155.0
13	1113.0
14	1071.0
15	1036.0
16	1001.0
17	997.5
18	994.0
19	1013.5
20	1033.0
21	1213.0
22	1393.0
23	1329.0
24	1265.0
25	1912.0
26	3015.5
27	3472.0
28	4132.0
29	4792.0
30	5195.0
31	5598.0
32	6254.5
33	6911.0
34	7628.5
35	8346.0
36	8699.0
37	9052.0
38	9852.5
39	10653.0
40	12428.5
41	14204.0
42	16637.5
43	19071.0
44	20387.0
45	21703.0
46	27390.5
47	33078.0
48	34795.5
49	36513.0
50	35898.0
51	35283.0
52	31536.5
53	27790.0
54	24403.5
55	21017.0
56	19339.5
57	17662.0
58	16931.0
59	16200.0
60	15057.5
61	13915.0
62	13162.0
63	12409.0
64	10900.0
65	9391.0
66	8219.5
67	7048.0
68	6004.5
69	4961.0
70	4385.5
71	3810.0
72	3192.5
73	2575.0
74	2156.5
75	1312.0
76	886.0
77	672.0
78	458.0
79	417.0
80	376.0
81	270.0
82	164.0
83	121.5
84	79.0
85	48.5
86	18.0
87	10.0
88	2.0
89	3.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	367027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.938459290797695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.66832780248413	25.389194126574345
2	9.17868707006823	6.413783690811002
3	3.4022468738382416	3.5660779169652344
4	2.03805441494532	2.848259248359903
5	1.4179925457525915	2.477123741721576
6	1.186875440524978	2.4880559557217716
7	1.034765180293254	2.530717078904756
8	0.8846029731547689	2.47253319728692
9	0.8345613017150558	2.6242457459081954
>10	7.226515997854686	42.19512182696975
>50	0.07657852231987476	1.7975230907299793
>100	0.044540569104416956	2.9193369394252233
>500	0.003907067465299733	1.0947854790138545
>1k	0.0023442404791798395	1.1832419616074925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1852	0.5045950297934485	No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	1358	0.37000002724595193	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCG	1124	0.3062444997234536	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGC	964	0.26265097663114706	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTC	951	0.25910900287989713	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC	826	0.22505156296403261	No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT	637	0.1735567138112455	No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC	632	0.17219441621461093	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	454	0.12369662177441987	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	374	0.1018998602282666	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1820029589103799	0.0
2	0.0	0.0	0.0	0.9339912322526681	0.0
3	0.0	0.0	0.0	1.284374174107082	0.0
4	0.0	0.0	0.0	1.8080413702534146	0.0
5	0.0	0.0	0.0	3.335449435600106	0.0
6	0.0	0.0	0.0	4.117953175107009	0.0
7	0.0	0.0	0.0	4.92443335231468	0.0
8	0.0	0.0	0.0	6.069580712045708	0.0
9	0.0	0.0	0.0	6.575265579916464	0.0
10	2.72459519326916E-4	0.0	0.0	7.930751688567871	0.0
11	2.72459519326916E-4	0.0	0.0	9.426281990153313	0.0
12	2.72459519326916E-4	0.0	0.0	10.810648807853374	0.0
13	2.72459519326916E-4	0.0	0.0	11.274647369267111	0.0
14	2.72459519326916E-4	0.0	0.0	11.483896280110182	0.0
15	2.72459519326916E-4	0.0	0.0	11.913837401608056	0.0
16	2.72459519326916E-4	0.0	0.0	12.67318208197217	0.0
17	2.72459519326916E-4	0.0	0.0	13.531429567851957	0.0
18	2.72459519326916E-4	0.0	0.0	14.46051652875674	0.0
19	2.72459519326916E-4	0.0	0.0	15.074912744838935	0.0
20	2.72459519326916E-4	0.0	0.0	15.62936786666921	0.0
21	2.72459519326916E-4	0.0	0.0	16.2821808749765	0.0
22	2.72459519326916E-4	0.0	0.0	16.94398504742158	0.0
23	2.72459519326916E-4	0.0	0.0	17.630583036125408	0.0
24	2.72459519326916E-4	0.0	0.0	18.16324139640953	0.0
25	2.72459519326916E-4	0.0	0.0	18.615796658011536	0.0
26	2.72459519326916E-4	0.0	0.0	19.060995512591717	0.0
27	2.72459519326916E-4	0.0	0.0	19.500745176785358	0.0
28	2.72459519326916E-4	0.0	0.0	19.950303383674772	0.0
29	2.72459519326916E-4	0.0	0.0	20.39277764306168	0.0
30	2.72459519326916E-4	0.0	0.0	20.893285780065227	0.0
31	5.44919038653832E-4	0.0	0.0	21.342843986954637	0.0
32	5.44919038653832E-4	0.0	0.0	21.80466287221376	0.0
33	5.44919038653832E-4	0.0	0.0	22.25531091718048	0.0
34	5.44919038653832E-4	0.0	0.0	22.710045854937103	0.0
35	5.44919038653832E-4	0.0	0.0	23.178403768660072	0.0
36	5.44919038653832E-4	0.0	0.0	23.630414111223423	0.0
37	5.44919038653832E-4	0.0	0.0	24.067711639743123	0.0
38	5.44919038653832E-4	0.0	0.0	24.489206516141863	0.0
39	5.44919038653832E-4	0.0	0.0	24.928956180335508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0270046E-4	45.0	1
CGGCGAA	20	7.0270046E-4	45.0	31
CGATTGT	25	3.8855993E-5	45.0	10
CAATTAG	25	3.8855993E-5	45.0	44
CGATTAT	20	7.0270046E-4	45.0	10
TTAAGCG	20	7.0270046E-4	45.0	1
GCGATAA	20	7.0270046E-4	45.0	9
GGTGATC	40	6.7939254E-9	45.0	8
CGATGAC	20	7.0270046E-4	45.0	10
CGAAGCT	20	7.0270046E-4	45.0	42
GTTAAGG	85	0.0	42.35294	2
GTAAGCG	85	0.0	42.35294	1
AGTACGG	70	0.0	41.785717	2
ACGTAGG	50	1.0768417E-9	40.5	2
GATGAAT	95	0.0	40.263157	20
CGTGCGG	45	1.9221261E-8	40.000004	2
TACGGGT	40	3.4496406E-7	39.375	4
ACGTGCG	35	6.2366707E-6	38.57143	1
CGTTTTT	870	0.0	38.53448	1
TACGAAT	65	9.094947E-12	38.076927	12
>>END_MODULE