FastQCFastQC Report
Sat 18 Jun 2016
SRR3551470_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551470_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243773
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG21120.8663797877533608No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC19160.7859771180565526No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC18310.7511086133411001No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14870.6099937236691513No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC13470.5525632453142882TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC6460.26500063583743894TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT6190.2539247578690011TruSeq Adapter, Index 20 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT5200.2133132053180623No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC5120.2100314636977844No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA3350.1374229303491363No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT3210.13167988251365No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC3200.13126966481111527No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT3000.12306531076042054TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC2930.1201937868426774TruSeq Adapter, Index 23 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2870.11773248062746901No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2800.11486095670972586No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2650.10870769117170481No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAT302.158442E-645.0000048
ATGGGCG253.881735E-545.05
AAGTAAG207.0223416E-445.02
AAGTAAC207.0223416E-445.019
TTAATCG253.881735E-545.020
CAACGCG207.0223416E-445.01
CGTTGAT253.881735E-545.025
GAATCCG253.881735E-545.09
ATTCGCG207.0223416E-445.01
CGATTAG207.0223416E-445.010
TACGGGA502.1827873E-1145.04
TAACGGG502.1827873E-1145.03
TATGCGG406.7775545E-945.02
ACTTACG207.0223416E-445.01
TTATCCG207.0223416E-445.01
TCTAGCG253.881735E-545.01
TGCGAAG207.0223416E-445.01
TGGGACG253.881735E-545.06
TATCCGG207.0223416E-445.02
TAATCGT253.881735E-545.021
Produced by FastQC (version 0.11.5)