##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551470_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243773
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.19020564213428	33.0	31.0	34.0	30.0	34.0
2	32.25915503357632	34.0	31.0	34.0	30.0	34.0
3	32.278800359350704	34.0	31.0	34.0	30.0	34.0
4	35.86546910445373	37.0	35.0	37.0	35.0	37.0
5	35.91798517473223	37.0	35.0	37.0	35.0	37.0
6	35.893868476000215	37.0	35.0	37.0	35.0	37.0
7	36.075205211405695	37.0	35.0	37.0	35.0	37.0
8	35.86614186148589	37.0	35.0	37.0	35.0	37.0
9	37.75806590557609	39.0	38.0	39.0	35.0	39.0
10	37.468903447059354	39.0	37.0	39.0	35.0	39.0
11	37.44631275818077	39.0	37.0	39.0	35.0	39.0
12	37.3389382745423	39.0	37.0	39.0	35.0	39.0
13	37.28808358595907	39.0	37.0	39.0	34.0	39.0
14	38.481456108756916	40.0	38.0	41.0	34.0	41.0
15	38.52869267720379	40.0	38.0	41.0	34.0	41.0
16	38.58512222436447	40.0	38.0	41.0	35.0	41.0
17	38.52829066385531	40.0	38.0	41.0	34.0	41.0
18	38.436053213440374	40.0	38.0	41.0	34.0	41.0
19	38.36895800601379	40.0	37.0	41.0	34.0	41.0
20	38.23072694679066	40.0	37.0	41.0	34.0	41.0
21	38.182087433801115	40.0	37.0	41.0	34.0	41.0
22	38.18220229475783	40.0	37.0	41.0	34.0	41.0
23	38.13688964733584	40.0	37.0	41.0	34.0	41.0
24	38.07181681318276	40.0	36.0	41.0	34.0	41.0
25	37.94477649288478	40.0	36.0	41.0	34.0	41.0
26	37.96627600267462	40.0	36.0	41.0	34.0	41.0
27	37.916299179974814	40.0	36.0	41.0	34.0	41.0
28	37.843362472464136	40.0	36.0	41.0	33.0	41.0
29	37.8124771816403	40.0	36.0	41.0	33.0	41.0
30	37.66084020789833	40.0	36.0	41.0	33.0	41.0
31	37.62964315162056	40.0	36.0	41.0	33.0	41.0
32	37.4968926009033	40.0	36.0	41.0	33.0	41.0
33	37.36234529664893	40.0	36.0	41.0	33.0	41.0
34	37.24487945752811	40.0	35.0	41.0	33.0	41.0
35	37.154779241343384	40.0	35.0	41.0	32.0	41.0
36	37.054673815393826	40.0	35.0	41.0	32.0	41.0
37	37.01137123471426	40.0	35.0	41.0	32.0	41.0
38	36.90405418155415	40.0	35.0	41.0	31.0	41.0
39	36.84177903213235	39.0	35.0	41.0	31.0	41.0
40	36.77041756059941	39.0	35.0	41.0	31.0	41.0
41	36.645998531420624	39.0	35.0	41.0	31.0	41.0
42	36.52048011879905	39.0	35.0	41.0	31.0	41.0
43	36.37454927329934	39.0	35.0	41.0	31.0	41.0
44	36.41879945687176	39.0	35.0	41.0	31.0	41.0
45	36.37625577894188	39.0	35.0	41.0	31.0	41.0
46	36.29126277315371	39.0	35.0	41.0	31.0	41.0
47	36.16492802730409	39.0	35.0	41.0	30.0	41.0
48	36.145184249281094	38.0	35.0	41.0	31.0	41.0
49	36.07635382097279	38.0	35.0	40.0	30.0	41.0
50	35.91450652861474	38.0	35.0	40.0	30.0	41.0
51	34.98418610756728	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	0.0
11	3.0
12	7.0
13	8.0
14	10.0
15	9.0
16	21.0
17	42.0
18	73.0
19	120.0
20	264.0
21	395.0
22	629.0
23	913.0
24	1140.0
25	1615.0
26	2231.0
27	2477.0
28	2569.0
29	2807.0
30	3174.0
31	4066.0
32	5229.0
33	7194.0
34	13371.0
35	19033.0
36	17360.0
37	26111.0
38	47304.0
39	85506.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.788610715706824	17.95358796913522	23.15843017889594	12.099371136262015
2	33.582472218006096	26.05456715879117	23.183863676453093	17.179096946749638
3	31.19705627776661	26.69286590393522	25.783003039713176	16.327074778584997
4	28.050686499325188	24.949030450460057	28.586430818835556	18.413852231379195
5	26.718709619194907	27.76722606687369	25.438830387286533	20.07523392664487
6	24.902265632371098	35.62699724743922	25.126654715657597	14.344082404532084
7	73.90523150636042	6.667268319297051	14.096721129903639	5.330779044438884
8	72.65037555430666	9.302917058082723	11.678077555758842	6.368629831851763
9	66.43352627239277	8.53416908353263	13.954375587124087	11.077929056950524
10	38.35330409848506	24.35298412867709	21.468743462155366	15.82496831068248
11	31.28976547853946	23.322517259909834	25.20131433751892	20.186402924031785
12	27.263888945863567	20.573648435224573	30.822527515352398	21.33993510355946
13	25.26694916992448	23.106332530674027	32.5208288038462	19.105889495555292
14	20.56626451657895	28.608172357069893	28.768567478760975	22.056995647590178
15	18.81053274973028	24.410824824734487	35.90020223732735	20.878440188207882
16	20.245474273196788	23.927998588851104	32.36658694769314	23.459940190258973
17	20.987968314784656	23.859492232527803	30.12433698563828	25.028202467049265
18	20.878440188207882	24.106033071751177	31.840277635341074	23.175249104699862
19	23.115357320129792	25.49544043023633	27.934594889507864	23.45460736012602
20	24.985129608283117	24.935083048573876	29.865079397636325	20.214707945506678
21	23.177710410915072	27.63718705517018	28.62663215368396	20.55847038023079
22	21.55570961509273	24.399338729063512	28.1794948579211	25.865456797922658
23	21.49130543579478	27.075599020400126	28.78579662226744	22.647298921537658
24	22.906556509539612	24.14582418889705	28.99295656204748	23.95466273951586
25	21.383007962325607	28.09047761647106	27.06985597256464	23.45665844863869
26	21.730052138669993	25.366221853937887	29.292415484897838	23.61131052249429
27	24.716437013122864	26.056208029601308	26.998478092323598	22.22887686495223
28	19.998523216270875	26.79706120037904	30.206790743847762	22.997624839502322
29	24.243866219802847	23.646999462614808	27.717589724866986	24.391544592715352
30	23.64002576167172	26.62600041842206	27.09323838160912	22.640735438297106
31	23.79016544079943	27.27496482383201	25.024100290023917	23.910769445344645
32	25.52661697562897	26.583747995060982	24.955183715998082	22.934451313311975
33	24.432566362968828	25.752646929725604	24.69264438637585	25.12214232092972
34	22.555820373872415	24.76279161350929	28.564689280601215	24.11669873201708
35	23.991172115041454	23.29872463316282	27.161334520229886	25.54876873156584
36	24.478920963355254	28.435880922005307	25.67429534854147	21.41090276609797
37	22.724009631911652	26.578415164928025	28.488388787929754	22.209186415230565
38	20.3578739236913	27.831630246171645	25.457290183900593	26.353205646236454
39	22.029921279222883	24.602396491818208	28.025253001768036	25.34242922719087
40	22.40034786461175	23.317594647479417	31.217156945190812	23.06490054271802
41	20.618772382503394	26.188708347520027	28.798923588748547	24.393595681228028
42	22.267437328990493	25.09875991188524	29.82898023981327	22.804822519311
43	23.533779376715223	25.257103945063648	26.77614009754977	24.43297658067136
44	22.526284699289914	24.191358353878403	27.73604952148105	25.546307425350633
45	22.850356684292354	22.87702083495711	27.447256258896598	26.825366221853937
46	24.206126191169652	25.689473403535256	26.983710255032346	23.120690150262742
47	20.04241651044209	25.017947024485892	31.81566457318899	23.123971891883023
48	21.853527667132948	23.8488265722619	28.871121904394663	25.426523856210494
49	21.171335627817683	22.807283825526206	31.74264582213781	24.2787347245183
50	21.11554602027296	23.119049279452604	29.619358993818018	26.146045706456416
51	21.153286048906157	22.790875117424818	27.22163652250249	28.834202311166536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	322.5
2	508.0
3	1461.0
4	2414.0
5	1606.0
6	798.0
7	794.0
8	790.0
9	759.0
10	728.0
11	751.0
12	774.0
13	777.5
14	781.0
15	773.0
16	765.0
17	796.5
18	828.0
19	826.5
20	825.0
21	841.0
22	857.0
23	872.0
24	887.0
25	1169.5
26	1728.5
27	2005.0
28	2145.5
29	2286.0
30	2825.5
31	3365.0
32	3795.0
33	4225.0
34	4600.0
35	4975.0
36	5369.0
37	5763.0
38	6278.0
39	6793.0
40	7883.5
41	8974.0
42	10112.5
43	11251.0
44	12898.5
45	14546.0
46	20762.0
47	26978.0
48	25340.5
49	23703.0
50	24086.5
51	24470.0
52	21254.5
53	18039.0
54	15868.5
55	13698.0
56	12988.0
57	12278.0
58	11335.0
59	10392.0
60	9968.0
61	9544.0
62	8994.5
63	8445.0
64	7299.0
65	6153.0
66	5235.0
67	4317.0
68	3758.5
69	3200.0
70	2640.5
71	2081.0
72	1774.0
73	1467.0
74	1223.5
75	786.5
76	593.0
77	489.5
78	386.0
79	277.5
80	169.0
81	128.0
82	87.0
83	60.5
84	34.0
85	26.5
86	19.0
87	14.0
88	9.0
89	5.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	243773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.80104031209363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95314955802522	26.847517977790815
2	9.196196675992912	6.76859209182313
3	3.521307309025649	3.8876331669216855
4	2.0733243414965834	3.0520197068584296
5	1.5784017567522377	2.9043413339459248
6	1.2540268194535786	2.7689694921094623
7	1.1224933397242256	2.891624585167348
8	0.9374547156981864	2.7599447026536983
9	0.8794908093767765	2.9129559056991545
>10	6.372685623836542	36.870367103822
>50	0.07134019239558137	1.7951126662920012
>100	0.03009664366688589	2.032628716059613
>500	0.004458762024723836	0.9422700627222866
>1k	0.0055734525309047945	3.566022488134453
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCG	2112	0.8663797877533608	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTC	1916	0.7859771180565526	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGC	1831	0.7511086133411001	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1487	0.6099937236691513	No Hit
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1347	0.5525632453142882	TruSeq Adapter, Index 23 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	646	0.26500063583743894	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	619	0.2539247578690011	TruSeq Adapter, Index 20 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCT	520	0.2133132053180623	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCC	512	0.2100314636977844	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGACCTTATCGTA	335	0.1374229303491363	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGACCTTAT	321	0.13167988251365	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTC	320	0.13126966481111527	No Hit
GAACTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCT	300	0.12306531076042054	TruSeq Adapter, Index 20 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	293	0.1201937868426774	TruSeq Adapter, Index 23 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	287	0.11773248062746901	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	280	0.11486095670972586	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	265	0.10870769117170481	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.204354050694704E-4	0.0	0.0	0.26335976502729996	0.0
2	8.204354050694704E-4	0.0	0.0	1.305722947168062	0.0
3	8.204354050694704E-4	0.0	0.0	1.7508091544182498	0.0
4	8.204354050694704E-4	0.0	0.0	2.4432566362968826	0.0
5	8.204354050694704E-4	0.0	0.0	4.643254174990668	0.0
6	8.204354050694704E-4	0.0	0.0	5.4362049939903105	0.0
7	8.204354050694704E-4	0.0	0.0	6.470363822080378	0.0
8	8.204354050694704E-4	0.0	0.0	7.943455591882612	0.0
9	8.204354050694704E-4	0.0	0.0	8.520221681646449	0.0
10	8.204354050694704E-4	0.0	0.0	10.562695622566896	0.0
11	8.204354050694704E-4	0.0	0.0	12.221616011617366	0.0
12	8.204354050694704E-4	0.0	0.0	14.171790969467496	0.0
13	8.204354050694704E-4	0.0	0.0	14.703022894249978	0.0
14	8.204354050694704E-4	0.0	0.0	14.936436766992243	0.0
15	8.204354050694704E-4	0.0	0.0	15.472591304205142	0.0
16	8.204354050694704E-4	0.0	0.0	16.27374647725548	0.0
17	8.204354050694704E-4	0.0	0.0	17.13684452338856	0.0
18	8.204354050694704E-4	0.0	0.0	18.168542045263422	0.0
19	8.204354050694704E-4	0.0	0.0	18.828992546344345	0.0
20	8.204354050694704E-4	0.0	0.0	19.42298777961464	0.0
21	8.204354050694704E-4	0.0	0.0	20.07113174961952	0.0
22	8.204354050694704E-4	0.0	0.0	20.74224791096635	0.0
23	8.204354050694704E-4	0.0	0.0	21.417466249338524	0.0
24	8.204354050694704E-4	0.0	0.0	21.898241396709235	0.0
25	8.204354050694704E-4	0.0	0.0	22.34332760395942	0.0
26	8.204354050694704E-4	0.0	0.0	22.761339442842317	0.0
27	8.204354050694704E-4	0.0	0.0	23.179761499427745	0.0
28	8.204354050694704E-4	0.0	0.0	23.58915876655741	0.0
29	8.204354050694704E-4	0.0	0.0	24.014554524085934	0.0
30	8.204354050694704E-4	0.0	0.0	24.493688800646503	0.0
31	8.204354050694704E-4	0.0	0.0	24.93508304857388	0.0
32	0.0012306531076042054	0.0	0.0	25.34489053340608	0.0
33	0.0012306531076042054	0.0	0.0	25.718188642712686	0.0
34	0.0012306531076042054	0.0	0.0	26.15137853658937	0.0
35	0.0012306531076042054	0.0	0.0	26.596464743839555	0.0
36	0.0012306531076042054	0.0	0.0	27.039910080279604	0.0
37	0.0012306531076042054	0.0	0.0	27.44930734740927	0.0
38	0.0012306531076042054	0.0	0.0	27.843116341842617	0.0
39	0.0012306531076042054	0.0	0.0	28.228310764522732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCTAT	30	2.158442E-6	45.000004	8
ATGGGCG	25	3.881735E-5	45.0	5
AAGTAAG	20	7.0223416E-4	45.0	2
AAGTAAC	20	7.0223416E-4	45.0	19
TTAATCG	25	3.881735E-5	45.0	20
CAACGCG	20	7.0223416E-4	45.0	1
CGTTGAT	25	3.881735E-5	45.0	25
GAATCCG	25	3.881735E-5	45.0	9
ATTCGCG	20	7.0223416E-4	45.0	1
CGATTAG	20	7.0223416E-4	45.0	10
TACGGGA	50	2.1827873E-11	45.0	4
TAACGGG	50	2.1827873E-11	45.0	3
TATGCGG	40	6.7775545E-9	45.0	2
ACTTACG	20	7.0223416E-4	45.0	1
TTATCCG	20	7.0223416E-4	45.0	1
TCTAGCG	25	3.881735E-5	45.0	1
TGCGAAG	20	7.0223416E-4	45.0	1
TGGGACG	25	3.881735E-5	45.0	6
TATCCGG	20	7.0223416E-4	45.0	2
TAATCGT	25	3.881735E-5	45.0	21
>>END_MODULE