##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3551469_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 562861 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26420732649802 33.0 31.0 34.0 30.0 34.0 2 32.347595587542926 34.0 31.0 34.0 30.0 34.0 3 32.34782299715205 34.0 31.0 34.0 30.0 34.0 4 35.912251870355206 37.0 35.0 37.0 35.0 37.0 5 35.97616107706876 37.0 35.0 37.0 35.0 37.0 6 35.96318984616095 37.0 35.0 37.0 35.0 37.0 7 36.14005944629314 37.0 35.0 37.0 35.0 37.0 8 35.95652034871842 37.0 35.0 37.0 35.0 37.0 9 37.85635885236319 39.0 38.0 39.0 35.0 39.0 10 37.5417287749551 39.0 37.0 39.0 35.0 39.0 11 37.529214139903104 39.0 37.0 39.0 35.0 39.0 12 37.41130225757336 39.0 37.0 39.0 35.0 39.0 13 37.39460541767861 39.0 37.0 39.0 35.0 39.0 14 38.61233235203718 40.0 38.0 41.0 35.0 41.0 15 38.65298181966773 40.0 38.0 41.0 35.0 41.0 16 38.63928394399328 40.0 38.0 41.0 35.0 41.0 17 38.58488152492356 40.0 38.0 41.0 34.0 41.0 18 38.49346286205653 40.0 38.0 41.0 34.0 41.0 19 38.443503813552546 40.0 37.0 41.0 34.0 41.0 20 38.36685433881544 40.0 37.0 41.0 34.0 41.0 21 38.266367717784675 40.0 37.0 41.0 34.0 41.0 22 38.287735337854286 40.0 37.0 41.0 34.0 41.0 23 38.255992154368485 40.0 37.0 41.0 34.0 41.0 24 38.192187769271634 40.0 37.0 41.0 34.0 41.0 25 38.02834625244954 40.0 36.0 41.0 34.0 41.0 26 38.06369068029229 40.0 36.0 41.0 34.0 41.0 27 38.07554085289263 40.0 36.0 41.0 34.0 41.0 28 38.00314464850114 40.0 36.0 41.0 34.0 41.0 29 37.97628011178604 40.0 36.0 41.0 34.0 41.0 30 37.80416479379456 40.0 36.0 41.0 33.0 41.0 31 37.770055129063834 40.0 36.0 41.0 33.0 41.0 32 37.67658089652685 40.0 36.0 41.0 33.0 41.0 33 37.58346554477926 40.0 36.0 41.0 33.0 41.0 34 37.46350164605471 40.0 36.0 41.0 33.0 41.0 35 37.338561385493044 40.0 35.0 41.0 33.0 41.0 36 37.301132961779196 40.0 35.0 41.0 33.0 41.0 37 37.32354346810314 40.0 35.0 41.0 33.0 41.0 38 37.215594969273056 40.0 35.0 41.0 33.0 41.0 39 37.181343173536625 40.0 35.0 41.0 33.0 41.0 40 37.09610010286732 40.0 35.0 41.0 33.0 41.0 41 37.008993339385746 39.0 35.0 41.0 32.0 41.0 42 36.92978372990845 39.0 35.0 41.0 32.0 41.0 43 36.807812230728366 39.0 35.0 41.0 32.0 41.0 44 36.84070489872278 39.0 35.0 41.0 32.0 41.0 45 36.8184880458941 39.0 35.0 41.0 32.0 41.0 46 36.72747267975575 39.0 35.0 41.0 32.0 41.0 47 36.6806032039882 39.0 35.0 41.0 32.0 41.0 48 36.655751597641334 39.0 35.0 41.0 32.0 41.0 49 36.608071975141286 39.0 35.0 41.0 32.0 41.0 50 36.51756117407317 39.0 35.0 41.0 31.0 41.0 51 35.7287678485452 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 4.0 9 9.0 10 10.0 11 13.0 12 10.0 13 13.0 14 19.0 15 26.0 16 36.0 17 55.0 18 120.0 19 225.0 20 367.0 21 616.0 22 954.0 23 1382.0 24 1933.0 25 2878.0 26 4021.0 27 4827.0 28 5362.0 29 5982.0 30 6808.0 31 8678.0 32 11196.0 33 15783.0 34 32502.0 35 44688.0 36 39207.0 37 56733.0 38 104472.0 39 213704.0 40 226.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94879197528342 20.217424905971455 23.878008957806635 12.95577416093849 2 34.18463883623132 23.905369176404122 24.145570576039198 17.764421411325355 3 31.93132940459545 23.916561993103095 26.663066014522236 17.48904258777922 4 28.699980989977984 24.437294465240974 27.15857023314815 19.70415431163289 5 25.707057337424338 28.648636164168416 24.733282284613786 20.911024213793457 6 25.56972325316552 33.797154181938346 25.070132768125703 15.56298979677043 7 76.27567019210782 6.416504252382027 11.639463384387975 5.668362171122177 8 76.08752427331082 6.677314647843784 11.139162244319646 6.095998834525753 9 69.53670622054113 8.0485235253464 13.605135193235984 8.809635060876486 10 38.401843439143946 24.11483474605631 20.912623187607597 16.570698627192147 11 31.28143538102658 22.8715437736848 25.25934466946546 20.587676175823162 12 28.141228473815026 20.05930416212884 30.700297231465672 21.099170132590462 13 26.027385091523485 21.08389104947758 31.91640564899682 20.972318210002115 14 21.43690893488801 24.851428683102935 30.84473786600955 22.86692451599951 15 20.776710413405798 23.618975199916143 34.23100907684135 21.37330530983671 16 23.471514281501115 23.00710122037235 31.431561255798503 22.089823242328034 17 23.222074366495455 22.809716786204763 29.448833726266344 24.519375121033434 18 23.37379921508152 23.865572494807775 30.439131508489663 22.32149678162104 19 24.128337191597925 26.10502415338778 27.407299493125304 22.35933916188899 20 25.60134740193405 25.887030723393522 28.009401966027138 20.502219908645298 21 25.488530916158698 26.337586011466417 27.701688338683972 20.47219473369091 22 22.722306217698506 24.29640710583963 27.81059622180254 25.170690454659322 23 23.05560342606789 25.299319014818934 28.768736863985957 22.87634069512722 24 24.53500953166057 23.488037010913885 28.721833632104556 23.255119825320993 25 23.329383275799888 24.98432117343358 26.59128985664311 25.09500569412342 26 21.011581900327077 24.61549121363889 28.818482715981386 25.554444170052644 27 23.118141068576435 24.031688107721088 28.716503719390758 24.13366710431172 28 20.408591108639612 25.750407294163214 29.331930974077082 24.509070623120095 29 22.439110188838807 24.126738217783785 28.22171015579335 25.212441437584054 30 23.45552454335973 24.182524637521517 29.19903848374643 23.162912335372322 31 22.834234384688227 24.29694009711101 28.238765876477494 24.630059641723268 32 23.739431227247934 24.402827696358425 28.40505915314794 23.452681923245702 33 23.27288620103365 24.484908352150885 28.63762101122657 23.604584435588894 34 21.083180394449073 23.691817340338023 29.709288794213844 25.515713470999057 35 20.46704248473424 23.448240329317542 29.622944208250352 26.461772977697866 36 20.12290778718014 27.18202184908885 29.052110556602784 23.642959807128225 37 20.869095567111597 25.58891093893519 30.35616253391157 23.185830960041645 38 20.597625346222248 26.75829378834206 28.196126574767128 24.447954290668566 39 22.115406823354256 24.768992699796218 28.827010576323463 24.288589900526063 40 22.18149774100533 23.393164564608313 29.032034552047488 25.393303142338873 41 19.36446120800695 24.240798349859023 28.844776952036117 27.549963490097912 42 20.588031503337415 23.86788212365042 29.7732477467794 25.770838626232766 43 21.468355419899407 23.02131432094247 29.972764146032503 25.53756611312562 44 21.613151381957536 23.139460719431618 29.39144833271447 25.855939565896374 45 22.048605250674676 22.651063051090766 28.17551757894045 27.124814119294104 46 22.08751361348539 24.581735099784847 28.161482142127454 25.169269144602307 47 19.889102282801616 23.59854386784659 31.192781166220435 25.31957268313136 48 20.591584778479945 22.11718346092552 30.575932601477096 26.71529915911744 49 21.418609567903975 21.997615752379364 31.778716237223755 24.805058442492907 50 20.023949074460656 21.50211153375345 31.3819930675602 27.091946324225695 51 19.575881078987532 21.712998413462646 29.817841349818163 28.893279157731662 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 257.0 1 606.5 2 956.0 3 3571.5 4 6187.0 5 3957.0 6 1727.0 7 1680.0 8 1633.0 9 1587.0 10 1541.0 11 1540.5 12 1540.0 13 1494.5 14 1449.0 15 1359.5 16 1270.0 17 1365.5 18 1461.0 19 1429.5 20 1398.0 21 1543.5 22 1689.0 23 1924.0 24 2159.0 25 2590.0 26 3408.0 27 3795.0 28 4417.0 29 5039.0 30 5970.5 31 6902.0 32 7739.0 33 8576.0 34 10241.0 35 11906.0 36 12704.5 37 13503.0 38 14683.0 39 15863.0 40 18705.0 41 21547.0 42 24775.0 43 28003.0 44 37184.0 45 46365.0 46 50127.5 47 53890.0 48 53458.0 49 53026.0 50 47484.0 51 41942.0 52 38223.5 53 34505.0 54 32409.0 55 30313.0 56 30025.0 57 29737.0 58 28817.5 59 27898.0 60 28008.5 61 28119.0 62 25863.5 63 23608.0 64 20803.5 65 17999.0 66 14808.0 67 11617.0 68 9627.5 69 7638.0 70 6366.5 71 5095.0 72 4544.5 73 3994.0 74 3230.0 75 1951.5 76 1437.0 77 1189.5 78 942.0 79 721.0 80 500.0 81 350.0 82 200.0 83 156.5 84 113.0 85 66.0 86 19.0 87 13.5 88 8.0 89 5.5 90 3.0 91 2.5 92 2.0 93 1.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 562861.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.7850806164413 #Duplication Level Percentage of deduplicated Percentage of total 1 71.56964248487452 24.895557835262423 2 8.946152511560275 6.223852726432059 3 3.652473925725166 3.811547998673991 4 2.2384121551426017 3.1145338907783007 5 1.6858697213418699 2.9321557082847187 6 1.4455143346901684 3.016939959865141 7 1.219106091551587 2.9684692572231635 8 1.0688257214245533 2.974335110774329 9 0.9382588040316563 2.9373667323594264 >10 7.116903867654199 39.96702040203519 >50 0.07408231605410293 1.7702278995576428 >100 0.039099000139616535 2.7006175932267285 >500 0.0020578421126113966 0.5102021574640119 >1k 0.0036012236970699445 2.177172728062844 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3691 0.6557569275540498 No Hit GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC 2104 0.3738045449942348 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC 1626 0.2888812690877499 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG 1488 0.2643636706042877 No Hit CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC 1117 0.19845041671034233 No Hit CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT 1093 0.19418648653930545 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGC 1047 0.18601395371148471 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCC 927 0.16469430285630024 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 780 0.13857773055869924 No Hit TCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC 636 0.11299414953247781 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.329912713796124E-4 0.0 0.0 0.19951639925310158 0.0 2 7.106550285061498E-4 0.0 0.0 0.9878104896235482 0.0 3 7.106550285061498E-4 0.0 0.0 1.3836453405014737 0.0 4 7.106550285061498E-4 0.0 0.0 1.994275673745383 0.0 5 7.106550285061498E-4 0.0 0.0 3.6911422180609423 0.0 6 7.106550285061498E-4 0.0 0.0 4.732784826093831 0.0 7 7.106550285061498E-4 0.0 0.0 5.744046931658082 0.0 8 7.106550285061498E-4 0.0 0.0 7.234645853949732 0.0 9 7.106550285061498E-4 0.0 0.0 7.950986122683931 0.0 10 8.883187856326873E-4 0.0 0.0 9.436610459776038 0.0 11 8.883187856326873E-4 0.0 0.0 10.922057133111016 0.0 12 8.883187856326873E-4 0.0 0.0 12.194484961651279 0.0 13 8.883187856326873E-4 0.0 0.0 12.677908044792586 0.0 14 8.883187856326873E-4 0.0 0.0 12.88346501178799 0.0 15 8.883187856326873E-4 0.0 0.0 13.272726303652234 0.0 16 0.0010659825427592248 0.0 0.0 13.950868864604228 0.0 17 0.0010659825427592248 0.0 0.0 14.763325225943882 0.0 18 0.0010659825427592248 0.0 0.0 15.619664535293794 0.0 19 0.0010659825427592248 0.0 0.0 16.145016265116965 0.0 20 0.0010659825427592248 0.0 0.0 16.660596488298175 0.0 21 0.0010659825427592248 0.0 0.0 17.250084834444028 0.0 22 0.0010659825427592248 0.0 0.0 17.893049971484967 0.0 23 0.0010659825427592248 0.0 0.0 18.538680064882804 0.0 24 0.0010659825427592248 0.0 0.0 19.034717274780096 0.0 25 0.0010659825427592248 0.0 0.0 19.476034047482415 0.0 26 0.0010659825427592248 0.0 0.0 19.866183658132293 0.0 27 0.0010659825427592248 0.0 0.0 20.29630761413564 0.0 28 0.0010659825427592248 0.0 0.0 20.723411286267837 0.0 29 0.0010659825427592248 0.0 0.0 21.159042818742105 0.0 30 0.0010659825427592248 0.0 0.0 21.6698261204809 0.0 31 0.0010659825427592248 0.0 0.0 22.10812260931207 0.0 32 0.0010659825427592248 0.0 0.0 22.552637329642664 0.0 33 0.0010659825427592248 0.0 0.0 23.001060652630045 0.0 34 0.0010659825427592248 0.0 0.0 23.43651452134719 0.0 35 0.0010659825427592248 0.0 0.0 23.854379678108806 0.0 36 0.0010659825427592248 0.0 0.0 24.27988437642686 0.0 37 0.0010659825427592248 0.0 0.0 24.710185996187334 0.0 38 0.0010659825427592248 0.0 0.0 25.179218315001396 0.0 39 0.0010659825427592248 0.0 0.0 25.628707620531536 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGTCA 25 3.8882594E-5 45.000004 27 GGCGTAT 35 1.2104465E-7 45.0 6 GCGTATT 35 1.2104465E-7 45.0 7 TAGACGG 60 0.0 44.999996 2 TATTGCG 30 2.1635205E-6 44.999996 1 CGTTAGG 80 0.0 42.1875 2 CGTTTTT 1755 0.0 41.153847 1 GGCTACG 50 1.0786607E-9 40.500004 1 ATGGGCG 135 0.0 40.0 5 TCGGCGT 80 0.0 39.375 4 ACGTTAG 35 6.2426097E-6 38.571426 1 CGTGTTA 35 6.2426097E-6 38.571426 37 ATTAGTC 65 9.094947E-12 38.07692 31 GCTACGA 95 0.0 37.894737 10 ATTAGCG 30 1.13918206E-4 37.499996 1 TAAACGG 30 1.13918206E-4 37.499996 2 CCGTAGG 30 1.13918206E-4 37.499996 1 ACGCTAG 30 1.13918206E-4 37.499996 1 CGATGTC 30 1.13918206E-4 37.499996 10 CATATCA 85 0.0 37.058823 29 >>END_MODULE