FastQCFastQC Report
Sat 18 Jun 2016
SRR3551468_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551468_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences591964
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182333.0800859511727063No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC15930.26910420228257126No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC15770.26640133521633075No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG11810.19950537532687798No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC10690.18058530586319438No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT9380.15845558175835017No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC8750.14781304268502815No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC7480.12635903534674406No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG7220.12196687636410322No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT6600.11149326648242124No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC6440.10879039941618072No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG6140.10372252366697973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAACG253.8885046E-545.0000041
ATAGACG207.030511E-445.01
GCCGCTA207.030511E-445.044
TACGTGC207.030511E-445.037
CTAACGG302.1637115E-644.9999962
CGTTTTT75150.044.1017951
CCCCGAT1000.042.75000440
CCCTCGT556.002665E-1140.90909214
TGCAATC1050.040.71428734
TTTAGCG501.0804797E-940.5000041
TCGTTCG501.0804797E-940.50000445
TGTTGCG501.0804797E-940.5000041
GTTATAG501.0804797E-940.5000041
TCGTTCA950.040.26315716
GCGTAAG451.9255822E-840.01
CGTAAGG800.039.3752
TACCGGT403.4544246E-739.37540
TAACCCG356.2431573E-638.57142637
CCGACGG356.2431573E-638.5714262
TGATGCG356.2431573E-638.5714261