##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3551468_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	591964
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28227392206283	33.0	31.0	34.0	30.0	34.0
2	32.41836158955612	34.0	31.0	34.0	30.0	34.0
3	32.33400341912684	34.0	31.0	34.0	30.0	34.0
4	35.935810285760624	37.0	35.0	37.0	35.0	37.0
5	36.00905798325574	37.0	35.0	37.0	35.0	37.0
6	35.99888844591901	37.0	35.0	37.0	35.0	37.0
7	36.18244859484698	37.0	36.0	37.0	35.0	37.0
8	36.01103952267368	37.0	36.0	37.0	35.0	37.0
9	37.92686379577137	39.0	38.0	39.0	35.0	39.0
10	37.577317201721726	39.0	37.0	39.0	35.0	39.0
11	37.58124987330311	39.0	37.0	39.0	35.0	39.0
12	37.56045637910413	39.0	37.0	39.0	35.0	39.0
13	37.57567520997898	39.0	37.0	39.0	35.0	39.0
14	38.88720428945003	40.0	38.0	41.0	35.0	41.0
15	38.94100316911163	40.0	38.0	41.0	35.0	41.0
16	38.91356906838929	40.0	38.0	41.0	35.0	41.0
17	38.871073240940326	40.0	38.0	41.0	35.0	41.0
18	38.62643505348298	40.0	38.0	41.0	35.0	41.0
19	38.36369610314141	40.0	37.0	41.0	35.0	41.0
20	38.11110472934165	40.0	35.0	41.0	34.0	41.0
21	38.01274063963349	40.0	35.0	41.0	34.0	41.0
22	38.03146137265104	40.0	35.0	41.0	34.0	41.0
23	37.99636464379591	40.0	35.0	41.0	34.0	41.0
24	37.90390294004365	40.0	35.0	41.0	34.0	41.0
25	37.7528633497983	40.0	35.0	41.0	34.0	41.0
26	37.781012021001274	40.0	35.0	41.0	34.0	41.0
27	37.764178227054344	40.0	35.0	41.0	34.0	41.0
28	37.67171145542634	40.0	35.0	41.0	34.0	41.0
29	37.58814218432202	40.0	35.0	41.0	33.0	41.0
30	37.35280354886446	40.0	35.0	41.0	33.0	41.0
31	37.170888770263055	40.0	35.0	41.0	33.0	41.0
32	36.82216992925245	40.0	35.0	41.0	32.0	41.0
33	36.3963061942956	40.0	35.0	41.0	30.0	41.0
34	36.0302214323844	40.0	35.0	41.0	27.0	41.0
35	35.785257211587194	39.0	35.0	41.0	25.0	41.0
36	35.620507328148335	39.0	35.0	41.0	24.0	41.0
37	35.56021649965201	39.0	35.0	41.0	23.0	41.0
38	35.45136528572684	39.0	35.0	41.0	23.0	41.0
39	35.40487090431175	39.0	35.0	41.0	23.0	41.0
40	35.336577900007434	39.0	35.0	41.0	23.0	41.0
41	35.23374732247231	39.0	35.0	41.0	23.0	41.0
42	35.08712523058835	39.0	35.0	41.0	22.0	41.0
43	34.97532789156097	39.0	35.0	41.0	21.0	41.0
44	35.00391746795413	39.0	35.0	41.0	21.0	41.0
45	34.97638538830064	39.0	35.0	41.0	22.0	41.0
46	34.90556013541364	39.0	35.0	41.0	22.0	41.0
47	34.85758424498787	38.0	35.0	41.0	22.0	41.0
48	34.80537836760344	38.0	35.0	41.0	22.0	41.0
49	34.75171125271132	38.0	35.0	41.0	20.0	41.0
50	34.66371096891027	38.0	35.0	41.0	20.0	41.0
51	33.901657871086755	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	13.0
11	10.0
12	10.0
13	19.0
14	21.0
15	23.0
16	62.0
17	111.0
18	182.0
19	377.0
20	666.0
21	1034.0
22	1595.0
23	2407.0
24	4053.0
25	7530.0
26	11744.0
27	13412.0
28	12415.0
29	10523.0
30	9901.0
31	10451.0
32	12230.0
33	15602.0
34	27280.0
35	41240.0
36	44123.0
37	55708.0
38	100681.0
39	208249.0
40	280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.98698569507605	19.361988229013928	22.78905473981526	21.86197133609476
2	41.552695772040195	22.42382982748951	20.937252941057228	15.086221459413071
3	28.074511287848587	21.715002939367935	34.63572109114744	15.574764681636044
4	25.458811684494325	22.26402281219804	34.955504050922016	17.321661452385616
5	22.771992891459618	26.31190410227649	32.79202789358812	18.124075112675772
6	22.668101438600996	31.475393773945715	32.53762053097823	13.318884256475055
7	69.69376516139495	5.6821022900041225	20.16186795142948	4.462264597171449
8	70.07740335560946	6.001716320587063	19.292389402058234	4.628490921745241
9	63.9723023697387	7.685940361238183	21.333222966261463	7.008534302761654
10	33.812022352710635	21.926840145684533	29.27154353980985	14.989593961794975
11	26.445696022055394	22.63448452946463	32.56127061780784	18.358548830672134
12	22.91896128818644	20.140076085707914	37.6232338453014	19.317728780804238
13	21.63290335223088	21.401301430492396	39.033961524687314	17.931833692589414
14	19.28546330520099	24.298437067118947	37.31155948672554	19.10454014095452
15	18.611097972173983	23.2264124169713	39.198667486536344	18.963822124318373
16	20.2801183855775	22.364535681223856	37.7852369400842	19.570108993114445
17	20.522024988006027	23.342297842436363	35.2183578731139	20.917319296443704
18	20.615273901791326	23.365947929265968	36.107432208715394	19.91134596022731
19	20.880154874282898	24.918407200437866	34.659371177977036	19.5420667473022
20	21.173922738544913	24.338642214729276	35.25754944557439	19.229885601151423
21	21.021041820110682	25.132271557054146	34.93371218520045	18.912974437634723
22	19.979593353649886	23.318140968031837	34.740795048347536	21.96147062997074
23	19.74241676858728	24.534600077031712	34.82306356467623	20.89991958970478
24	19.464021460764506	24.31600570304951	35.7023400071626	20.517632829023384
25	20.26474582913826	25.62081477927712	33.857126446878524	20.257312944706097
26	19.232081680642743	24.63325472494949	34.70734706840281	21.42731652600496
27	20.680480569764377	24.026292139386854	34.725591421099935	20.567635869748834
28	19.340027434100723	24.517031441101146	34.96040299747958	21.182538127318555
29	19.673831516781426	23.73691643410748	34.09531660709097	22.49393544202012
30	19.722652053165397	25.018582211080403	34.251069321783085	21.007696413971118
31	20.545337216452353	25.660513139312524	32.31699900669635	21.47715063753877
32	19.836679257522416	25.932151279469696	31.733517578771682	22.497651884236202
33	19.386989749376653	26.756694663864693	31.40157171719902	22.454743869559636
34	18.459230628889596	26.72730098451933	32.02779223060862	22.785676155982458
35	19.195424046056857	25.58263678196647	31.394476691150135	23.827462480826537
36	19.52230203188032	28.893480008919457	30.15386070774574	21.43035725145448
37	19.183936861025334	28.556297342405955	31.023677115500263	21.236088681068445
38	18.7972579413613	28.964768127791558	30.00402051476103	22.233953416086113
39	19.95999756741964	27.212127764526223	30.476515463778203	22.351359204275937
40	19.728395645681157	26.114594806440934	32.547756282476634	21.609253265401275
41	18.94642241757945	26.66293896250448	31.354440472731447	23.036198147184624
42	18.80638011770986	27.42751248386726	31.326567156110848	22.439540242312034
43	19.677547958997508	26.243487779662278	31.643985107202464	22.434979154137753
44	20.549222587860072	25.985363974836307	30.849680048111033	22.615733389192584
45	20.344649336783995	25.391240007838316	30.51131487725605	23.752795778121644
46	20.414079234548048	26.552121412788615	31.028744991249468	22.00505436141387
47	19.544600685176803	25.631457318350442	32.919400504084706	21.90454149238805
48	18.817867302741384	25.883668601469008	31.98150563209925	23.31695846369036
49	19.27127325310323	25.27282064449865	32.90115615138758	22.554749951010532
50	18.85063956591955	24.244717584177415	33.263509267455454	23.641133582447583
51	18.777155367556137	24.672108439026697	31.653276212742664	24.8974599806745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	375.0
1	716.5
2	1058.0
3	13213.0
4	25368.0
5	15897.0
6	6426.0
7	6370.5
8	6315.0
9	6334.5
10	6354.0
11	6379.0
12	6404.0
13	6257.5
14	6111.0
15	5887.5
16	5664.0
17	5216.0
18	4768.0
19	4506.5
20	4245.0
21	4098.5
22	3952.0
23	3770.5
24	3589.0
25	4169.0
26	5301.5
27	5854.0
28	6611.5
29	7369.0
30	8471.5
31	9574.0
32	9963.0
33	10352.0
34	11385.5
35	12419.0
36	14008.0
37	15597.0
38	17122.0
39	18647.0
40	21312.5
41	23978.0
42	27235.5
43	30493.0
44	38359.0
45	46225.0
46	48713.5
47	51202.0
48	50492.5
49	49783.0
50	44764.0
51	39745.0
52	36431.0
53	33117.0
54	30443.0
55	27769.0
56	26298.5
57	24828.0
58	23713.5
59	22599.0
60	21085.5
61	19572.0
62	18329.0
63	17086.0
64	14959.0
65	12832.0
66	10836.5
67	8841.0
68	7477.5
69	6114.0
70	5459.5
71	4805.0
72	4058.5
73	3312.0
74	2685.0
75	1581.0
76	1104.0
77	903.5
78	703.0
79	544.5
80	386.0
81	258.0
82	130.0
83	93.5
84	57.0
85	37.5
86	18.0
87	14.5
88	11.0
89	7.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	591964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.757105523077776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.67359538439169	29.328895216235594
2	8.533642362047322	6.6147855504414945
3	3.4655187491343615	4.029404275572148
4	2.059083827754122	3.1921651677251766
5	1.4214118045892838	2.7544903651107644
6	1.137779957335201	2.6458234721089986
7	0.9200838285564306	2.4961850223407285
8	0.7684945017489861	2.3827697998552426
9	0.6266976323733168	2.1860087642060075
>10	5.299877860824783	34.97713524544424
>50	0.052606032477284084	1.3998456141027782
>100	0.03507068831257476	2.891779627276283
>500	0.003945452435164661	1.0819537406871547
>1k	0.0017535344156287382	0.9208839941150092
>5k	0.0	0.0
>10k+	4.3838360390718455E-4	3.097874144778406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18233	3.0800859511727063	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCC	1593	0.26910420228257126	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	1577	0.26640133521633075	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	1181	0.19950537532687798	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1069	0.18058530586319438	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT	938	0.15845558175835017	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC	875	0.14781304268502815	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	748	0.12635903534674406	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	722	0.12196687636410322	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT	660	0.11149326648242124	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGC	644	0.10879039941618072	No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG	614	0.10372252366697973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3785838328006433E-4	0.0	0.0	0.10118858579237927	0.0
2	3.3785838328006433E-4	0.0	0.0	0.47283280740045003	0.0
3	3.3785838328006433E-4	0.0	0.0	0.7875478914258299	0.0
4	3.3785838328006433E-4	0.0	0.0	1.2639282118507207	0.0
5	3.3785838328006433E-4	0.0	0.0	2.5058956287882372	0.0
6	3.3785838328006433E-4	0.0	0.0	3.672689555445939	0.0
7	3.3785838328006433E-4	0.0	0.0	4.5512902811657465	0.0
8	3.3785838328006433E-4	0.0	0.0	5.751194329384895	0.0
9	3.3785838328006433E-4	0.0	0.0	6.442452581575907	0.0
10	3.3785838328006433E-4	0.0	0.0	7.697765404652985	0.0
11	3.3785838328006433E-4	0.0	0.0	8.938550317249021	0.0
12	3.3785838328006433E-4	0.0	0.0	9.9811812880513	0.0
13	3.3785838328006433E-4	0.0	0.0	10.417863248440783	0.0
14	3.3785838328006433E-4	0.0	0.0	10.624632579008184	0.0
15	3.3785838328006433E-4	0.0	0.0	10.897960011081755	0.0
16	3.3785838328006433E-4	0.0	0.0	11.39292254258705	0.0
17	3.3785838328006433E-4	0.0	0.0	11.942111344608794	0.0
18	3.3785838328006433E-4	0.0	0.0	12.589616936165037	0.0
19	3.3785838328006433E-4	0.0	0.0	12.987107324094033	0.0
20	3.3785838328006433E-4	0.0	0.0	13.353514740761263	0.0
21	3.3785838328006433E-4	0.0	0.0	13.779047374502504	0.0
22	3.3785838328006433E-4	0.0	0.0	14.23498726273895	0.0
23	5.067875749200965E-4	0.0	0.0	14.687210708759316	0.0
24	5.067875749200965E-4	0.0	0.0	15.058010284409187	0.0
25	5.067875749200965E-4	0.0	0.0	15.411241224128494	0.0
26	5.067875749200965E-4	0.0	0.0	15.720381644829754	0.0
27	5.067875749200965E-4	0.0	0.0	16.041853896520735	0.0
28	5.067875749200965E-4	0.0	0.0	16.368056165577638	0.0
29	5.067875749200965E-4	0.0	0.0	16.70794169915738	0.0
30	5.067875749200965E-4	0.0	0.0	17.11675034292626	0.0
31	5.067875749200965E-4	0.0	0.0	17.48231311363529	0.0
32	5.067875749200965E-4	0.0	0.0	17.822029718023394	0.0
33	5.067875749200965E-4	0.0	0.0	18.157523092620497	0.0
34	5.067875749200965E-4	0.0	0.0	18.49115824610956	0.0
35	5.067875749200965E-4	0.0	0.0	18.84793669885331	0.0
36	5.067875749200965E-4	0.0	0.0	19.199140488272935	0.0
37	5.067875749200965E-4	0.0	0.0	19.549499631734363	0.0
38	5.067875749200965E-4	0.0	0.0	19.90712273043631	0.0
39	5.067875749200965E-4	0.0	0.0	20.25883330743086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTAACG	25	3.8885046E-5	45.000004	1
ATAGACG	20	7.030511E-4	45.0	1
GCCGCTA	20	7.030511E-4	45.0	44
TACGTGC	20	7.030511E-4	45.0	37
CTAACGG	30	2.1637115E-6	44.999996	2
CGTTTTT	7515	0.0	44.101795	1
CCCCGAT	100	0.0	42.750004	40
CCCTCGT	55	6.002665E-11	40.909092	14
TGCAATC	105	0.0	40.714287	34
TTTAGCG	50	1.0804797E-9	40.500004	1
TCGTTCG	50	1.0804797E-9	40.500004	45
TGTTGCG	50	1.0804797E-9	40.500004	1
GTTATAG	50	1.0804797E-9	40.500004	1
TCGTTCA	95	0.0	40.263157	16
GCGTAAG	45	1.9255822E-8	40.0	1
CGTAAGG	80	0.0	39.375	2
TACCGGT	40	3.4544246E-7	39.375	40
TAACCCG	35	6.2431573E-6	38.571426	37
CCGACGG	35	6.2431573E-6	38.571426	2
TGATGCG	35	6.2431573E-6	38.571426	1
>>END_MODULE