FastQCFastQC Report
Sat 18 Jun 2016
SRR3551466_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3551466_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences488846
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29410.601620960384252No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC9440.19310784991592445No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9050.18512987730287248No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCC7700.15751381825769262No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC6380.13051144941351672No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5760.11782851859276745No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT5640.11537375778875147Illumina Single End Adapter 1 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5340.10923685577871149No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG5320.10882772897804216No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC253.8875038E-545.00000418
ACGTGCG253.8875038E-545.0000041
CAACGCG207.029304E-445.01
TCGTAAG207.029304E-445.01
ATCGGTT207.029304E-445.034
CGCGTAA207.029304E-445.031
TCGCGTA207.029304E-445.030
GCGTAAC207.029304E-445.032
TCTAGCG302.162933E-644.9999961
GCTACGA1250.044.99999610
CGAATAT1400.043.39285714
TACGAAT1350.043.33333212
CTACGAA1400.041.78571311
CATATTA556.002665E-1140.90909228
CGTTTTT13600.040.533091
AAGCTAC1500.039.08
GGATCGC356.2409254E-638.5714268
GACGCAT356.2409254E-638.57142625
CGCGACC1000.038.25000410
AGCTACG1500.037.59